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Effect of Aconiti Radix on the Toxicity Induced by Glucose Oxidase in Cultured Myocardial Cells  

Lee Jung Hun (School of Medicine, Wonkang University)
Lee Kang Chang (Graduate School of Oriental Medicine, Wonkang University)
Kim Sang Su (School of Medicine, Wonkang University)
Hong Gi Youn (School of Medicine, Wonkang University)
Oh Yeon Kyun (School of Medicine, Wonkang University)
Suk Seung Whan (School of Medicine, Wonkang University)
Lee Gap Sang (College of Life Science, Wonkang University)
Jin Bok Hee (Wonkwang Health Science College)
Shin Hong Chul (Wonkwang Health Science College)
Ryu Do Gon (College of Oriental Medicine, Wonkang University)
Park Seung Taeck (School of Medicine, Wonkang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.3, 2002 , pp. 495-498 More about this Journal
Abstract
Cytotoxicity of glucose oxidase(GO) and neuroprotective effect of Aconiti Radix(AKR) against GO-induced neurotoxicity were measured for elucidating the mechanism of cardiotoxicity on cultured mouse myocardial cells by MTT assay after myocardial cells were cultured for 24 hours at various concentrations of GO. GO was toxic in a time-and dose-dependent manner on cultured myocardial cells after myocardial cells were grown for 24 hours in media containing 5～40mU/ml GO. While, cultures were pretreated with 50 μg/ml AKR for 2 hours increased remarkably cell viability. From the above results, it is suggested that GO is toxic on cultured mouse myocardial cells by the decrease of cell viability, and herb medicine such as AKR is very effective in the prevention of myocardial toxicity induced by GO.
Keywords
Glucose oxidase; cultured myocardial cell; aconiti radix;
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