• Journal of Microbiology and Biotechnology
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• 제16권2호
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• pp.325-329
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• 2006

Leuconostoc mesenteroides B-742 fermentations with maltose as an acceptor were tested for glucooligosaccharides and mannitol co-production. Leuconostoc oligosaccharides were produced that were oligomers with a size range of DP 2 to 7 and were primarily DP 3, 4, 5, and 6, containing mainly ${\alpha}-1,4$ and ${\alpha}-1,6$ linkages. Maltose was linked to the reducing end of the isomaltosyl residues. The $Ca^{2+}$ form of cation-exchange column could separate glucooligosaccharides from byproducts.

• Journal of Microbiology and Biotechnology
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• 제28권9호
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• pp.1433-1442
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• 2018

To identify and quantify the effects of a combination of dietary $1{\times}10^8CFU/g$ Lactococcus lactis subsp. lactis I2 ($LI_2$) and 0.1% ${\beta}$-glucooligosaccharides (BGO) on the growth and immunity of olive flounder (Paralichthys olivaceus), a feeding experiment was conducted. Flounder ($14{\pm}0.5g$) were divided into two groups and fed control and synbiotic feeds for 8 weeks. Investigations were carried out on growth and feed utilization, innate immunity, serum biochemical parameters, intestinal lactic acid bacterial (LAB) viability, microvillus length, and changes in the expression levels of genes encoding pro-inflammatory cytokines (tumor necrosis factor $[TNF]-{\alpha}$, interleukin $[IL]-1{\beta}$, and IL-6). Results demonstrated the synbiotic diet had significantly better (p < 0.05) responses in terms of weight gain and specific growth rate, three innate immune parameters (respiratory burst, serum lysozyme, and superoxide dismutase), intestinal LAB viability, and the relative $TNF-{\alpha}$ expression level (p < 0.05). Moreover, after challenge with Streptococcus iniae ($1{\times}10^8CFU/ml$), the synbiotically fed group exhibited significantly higher (p < 0.05) protection against streptococcosis, validating the observed changes in immune parameters and induction of the cytokine-encoding gene. Therefore, according to the results of the present study, synbiotic feed ($LI_2+BGO$) increased growth, modulated innate immune parameters and protected olive flounder against streptococcosis.

• Bulletin of the Korean Chemical Society
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• 제30권11호
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• pp.2535-2541
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• 2009

Prebiotic isomaltooligosaccharide preparations contain $\alpha$-D-glucooligosaccharides comprising isomaltooligosaccharides (IMOs) and non-prebiotic maltooligosaccharides (MOs). They are both glucose oligosaccharides characterized by their degree of polymerization (DP) value (from 2 to $\sim$10), linkages types and positions (IMOs: $\alpha$-(1$\rightarrow$2, 3, 6 and in a lower proportion internal 1$\rightarrow$4) linkages, MOs: α-(1$\rightarrow$4) linkages). Their structure is the key factor for their prebiotic potential. In order to determine and elucidate the exact structure of unknown IMOs and MOs, unambiguous assignments of $^{13}C$ and $^1H$ chemical shifts of commercial standards, representative of IMOs and MOs diversity, have been determined using optimized standard one and two-dimensional experiments such as $^1H$ NMR, $^{13}C$ NMR, APT and ${^1}H-{^1}H$ COSY, TOCSY, NOESY and <$^1H-{^{13}}C$ heteronuclear HSQC, HSQC-TOCSY, and HMBC. Here we point out the differential effect of substitution by a glucose residue at different positions on chemical shifts of anomeric as well as ring carbons together with the effect of the reducing end configuration for low DP oligosaccharides and diasteroisotopic effect for H-6 protons. From this study, structural $^{13}C$ specific spectral features can be identified as tools for structural analysis of isomaltooligosaccharides.

• Journal of Microbiology and Biotechnology
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• 제11권4호
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• pp.700-703
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• 2001

The influence of process conditions on highly branched glucooligosaccharides production by mixed culture of Leuconostoc mesenteroides ATCC 13146 and Lipomyces starkeyi ATCC 74054 was studied. We divided the batch culture fermentations into two groups according to inoculation method. One-point inoculation was performed by coinoculation of L. mesenteroides and L. starkeyi at the ration of 10 to 1, and two-point inoculation by L. mesenteroides inoculation first and L. starkeyi inoculation after L. mesenteroides grew to the end of the log phase of growth. Two-point inoculation improved the yield of oligosaccharide by 1.5 to 20 fold more than one-point inoculation. In this process, the highest yield of oligosaccharides (48% of theoretical yield) and productivity (0.85 g/l/h) were obtained with starch as an initial substrate for L. starkeyi growth. The estimated composition of the end product consisted of 31.5% oligosaccharides, 17.6% dextran, and 46.5% mannitol.

• 한국식품과학회지
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• 제25권6호
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• pp.649-654
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• 1993

효소를 이용하여 전분을 수식하여 묵의 텍스쳐 특성을 개선하고 아울러 기능성 탄수화물을 첨가시켜 불성을 개선하고 건강식품화하고자 하였다. 전분의 분자수식을 위하여 maltogenic amylase를 이용하였으며 고구마 전분의 구조를 modification시켜 묵을 제조하고 또한 분지 글루코올리고당과 도토리 전분을 혼합하여 10% 전분겔을 제조한 후 Instron을 사용한 기계적 검사와 관능검사를 병행하여 텍스쳐 특성을 시험하였다. 고구마 전분에 maltogenic amylase를 0.02% 첨가하였을 때 텍스쳐 개선효과가 가장 켰으며 묵의 품질이 우수하였다. 이는 효소에 의해 긴 아밀로오스와 아밀로펙틴이 묵이 형성될 수 있는 길이로 잘라졌기 때문으로 생각된다. Bacillus licheniformis maltogenic amylase(BLMA)를 이용하여 제조한 분지글루코올리고당을 첨가한 경우, 12.5%를 첨가하였을 때 텍스쳐 특성이 가장 향상되었다. 도토리 전분을 혼합하여 전분겔을 제조한 경우에는 50%를 혼합하였을 때 가장 텍스쳐 특성이 향상되었고 100% 도토리만으로 제조한 전분겔보다 수응력이 우수하였다.

• 산업식품공학
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• 제21권4호
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• pp.383-390
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• 2017

국내 재래시장에서 수집한 발효식품 등에서 우수한 glucansucrase 활성을 나타내는 유산균주를 분리한 후 이 균주를 이용한 올리고당 생성의 최적 조건을 조사하였다. 배추김치로부터 분리된 유산균주 CCK940은 glucansucrase 활성이 918.2 mU/mL로 가장 높아 본 올리고당 생산을 위한 균주로 선정하였고, Leu. lactis CCK940로 동정 및 명명되었다. 선정된 Leu. lactis CCK940는 배지의 pH를 6.0으로 조정하고 공여체인 sucrose와 수용체인 maltose의 초기 농도를 각각 5% (w/v)와 10% (w/v)로 첨가한 후 배양 4시간과 8시간째에 sucrose를 5% (w/v) 첨가하는 것이 최적인 것으로 확인되었다. 최적 조건에 12시간 배양 시 Leu. lactis CCK940는 중합도가 2-4인 올리고당을 최소 4종류 생성하였다. 본 균주는 수용체 분자로서 fructose와 melibiose를 사용할 수 없었다.