Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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Oligosaccharide Production by Leuconostoc lactis CCK940 Which Has Glucansucrase Activity

Leuconostoc lactis CCK940의 Glucansucrase 활성에 의한 올리고당 생산 최적화

  • Lee, Sulhee (Department of Food Science and Biotechnology, Gachon University) ;
  • Park, Young-Seo (Department of Food Science and Biotechnology, Gachon University)
  • 이설희 (가천대학교 식품생물공학과) ;
  • 박영서 (가천대학교 식품생물공학과)
  • Received : 2017.10.31
  • Accepted : 2017.11.14
  • Published : 2017.11.30
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Abstract

Glucansucrase is an enzyme classified as a glycoside hydrolase (GH) 70 family, which catalyzes the synthesis of glucooligosaccharides with a low molecular weight using sucrose as a donor of D-glucopyranose and maltose as a carbohydrate acceptor. In this study, glucansucrase-producing lactic acid bacteria strain was isolated from the fermented foods collected in traditional markets, and the optimum conditions for the oligosaccharide production were investigated. The strain CCK940 isolated from Chinese cabbage kimchi was selected as an oligosaccharide-producing strain due to its high glucansucrase activity, with 918.2 mU/mL, and identified as Leuconostoc lactis. The optimum conditions for the production of oligosaccharides using Leu. lactis CCK940 were to adjust the initial pH to 6.0, add 5% (w/v) sucrose and 10% (w/v) maltose as a donor and acceptor molecules, respectively, and feed 5% (w/v) sucrose at 4 and 8 h of cultivation. When Leu. lactis CCK940 was cultured for 12 h at optimum conditions, at least four oligosaccharides with a polymerization degree of 2-4 were produced.

국내 재래시장에서 수집한 발효식품 등에서 우수한 glucansucrase 활성을 나타내는 유산균주를 분리한 후 이 균주를 이용한 올리고당 생성의 최적 조건을 조사하였다. 배추김치로부터 분리된 유산균주 CCK940은 glucansucrase 활성이 918.2 mU/mL로 가장 높아 본 올리고당 생산을 위한 균주로 선정하였고, Leu. lactis CCK940로 동정 및 명명되었다. 선정된 Leu. lactis CCK940는 배지의 pH를 6.0으로 조정하고 공여체인 sucrose와 수용체인 maltose의 초기 농도를 각각 5% (w/v)와 10% (w/v)로 첨가한 후 배양 4시간과 8시간째에 sucrose를 5% (w/v) 첨가하는 것이 최적인 것으로 확인되었다. 최적 조건에 12시간 배양 시 Leu. lactis CCK940는 중합도가 2-4인 올리고당을 최소 4종류 생성하였다. 본 균주는 수용체 분자로서 fructose와 melibiose를 사용할 수 없었다.

Keywords

Acknowledgement

Supported by : 농림축산식품부

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