• Title/Summary/Keyword: Glucomannan

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Protective Effect of Modified Glucomannans against Changes in Antioxidant Systems of Quail Egg and Embryo due to Aurofusarin Consumption

  • Dvorska, J.E.;Surai, P.F.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.3
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    • pp.434-440
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    • 2004
  • The aim of this study was to evaluate effects of modified glucomannan ($Mycosorb^{TM}$) on the antioxidant profile of egg yolk and tissues of newly hatched quail after aurofusarin inclusion in the maternal diet. Fifty-four 45 day-old Japanese quail were divided into three groups and were fed a corn-soya diet balanced in all nutrients ad libitum. The diet of the experimental quail was supplemented with aurofusarin at the level of 26.4 mg/kg feed in the form of Fusarium graminearum culture enriched with aurofusarin or with aurofusarin plus $Mycosorb^{TM}$ at 1 g/kg feed. Eggs obtained after 8 weeks of feeding were analysed and incubated in standard conditions of $37.5^{\circ}C$/55% RH. Samples of quail tissues were collected from newly hatched quail. The main carotenoids, retinol, retinyl esters and malondialdehyde were analysed by HPLC-based methods. Inclusion of aurofusarin in the maternal diet was associated with decreased carotenoid and vitamin A concentrations in egg yolk and liver of newly-hatched quail. Furthermore, lipid peroxidation in quail tissues was enhanced. Inclusion of modified glucomannan ($Mycosorb^{TM}$) in the toxin-contaminated diet provided a significant protective effect against changes in antioxidant composition in the egg yolk and liver. It is suggested that a combination of mycotoxin adsorbents and natural antioxidants could be the next step in counteracting mycotoxins in animal feed.

Extraction and Characterization of Aloe Glucomannan: Assessing Its Flocculation Capability

  • Hye Mi Kwon;Shin Young Lee;Won Hur
    • Applied Chemistry for Engineering
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    • v.34 no.6
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    • pp.626-632
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    • 2023
  • Acemannan, a highly acetylated glucomannan, was extracted from fresh Aloe vera leaves by ethanol fractionation, resulting in a concentration increase of more than threefold. The presence of acemannan was confirmed using FTIR and 1H NMR analysis, revealing an average molecular weight of 780 kDa. The flocculating activity of the fractionated aloe gel polysaccharide was assessed through settling tests in a 1% (w/v) bentonite suspension. The results demonstrated that the aloe polysaccharide exhibited remarkable stability within a temperature range of 20~70 ℃. The maximal flocculation rate at different pH levels ranged from 93% to 97%, with an optimal dose for maximum flocculation rate between 0.25 mg/mL. Notably, the minimum dose required for flocculation was achieved at a pH of 3, attributed to the compression of electrostatic repulsion on the surface of bentonite particles. However, the flocs obtained under acidic conditions were less dense and compact, exhibiting lower sedimentation velocity compared to those formed under neutral and alkaline pH conditions. Additionally, the addition of salt showed a slight synergistic effect on flocculation, significantly enhancing the sedimentation velocity. This investigation highlights the potential of Aloe vera polysaccharide as a natural and edible flocculant, offering promising applications in various industries.

Efficacy of Glucomannan-containing Yeast Product (Mycosorb®) and Hydrated Sodium Calcium Aluminosilicate in Preventing the Individual and Combined Toxicity of Aflatoxin and T-2 Toxin in Commercial Broilers

  • Girish, C.K.;Devegowda, G.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.6
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    • pp.877-883
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    • 2006
  • A feeding trial was conducted on commercial broilers for a period of 35 days to determine the individual and combined effects of aflatoxin (AF) and T-2 toxin (T-2) on performance, organ weights and immune status. The efficacy of dietary glucomannan-containing yeast product (GYP) ($Mycosorb^{(R)}$) and hydrated sodium calcium aluminosilicate (HSCAS) in preventing the adverse effects of aflatoxin and T-2 toxin was also evaluated. Twelve dietary treatments ($4{\times}3$ factorial) comprising two dietary levels each of AF (0 and 2 mg/kg), T-2 toxin (0 and 1 mg/kg), GYP (0 and 1 kg/ton) and HSCAS (0 and 10 kg/ton) were tested on 720 commercial broiler chickens divided at random into 36 replicates of 20 chicks each (10 males and 10 females). Weight gain and feed intake were recorded weekly. Organ morphology and antibody titers for Newcastle disease (ND) and infectious bursal disease (IBD) were measured on the $35^{th}$ day. AF and T-2 toxin individually decreased weight gain and increased feed conversion ratio (FCR) (p<0.05). AF alone (p<0.05) increased weights of liver, kidney, gizzard and spleen and reduced thymus and bursal weights. T-2 toxin (p<0.05) increased liver and gizzard weights and decreased thymus weight. Both AF and T-2 toxin when fed individually affected ND and IBD titers in a significant manner. Significant interactions between AF and T-2 toxin were observed for their additive effects on weight gain, FCR, organ weights and antibody titers. Addition of GYP (p<0.05) improved weight gain, feed conversion efficiency and restored the organ weights. Antibody titers against ND and IBD were significantly improved with the supplementation of GYP. Supplementation of HSCAS (p<0.05) resulted in improvement in weight gain and restored organ weights in the groups fed AF alone, but not in T-2 toxin fed groups. HSCAS inclusion did not influence FCR in toxin fed groups. Addition of HSCAS (p<0.05) improved the antibody titers against ND and IBD only in AF fed groups. Thus, the results indicate that addition of GYP is effective in averting the individual and combined toxicity of aflatoxin and T-2 toxin in commercial broilers, while HSCAS is effective only against aflatoxin.

Several Functional Properties of Freeze-dried Powder of Pre-concentrated Aloe vera gel (농축 전처리된 Aloe vera gel의 동결건조분말의 기능성)

  • Lee, Nam Jae;Lee, Seung Ju
    • Food Engineering Progress
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    • v.13 no.3
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    • pp.216-220
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    • 2009
  • The several functional properties of freeze-dried powder of Aloe vera gel was examined as influenced by pre-concentration degrees of the gel solution. As a pre-treatment prior to freeze drying, the gel solution was vacuum-concentrated by three levels (unit: g-water/g-solids) - high (H), 76; medium (M), 119; low (L), 159. In FT-IR spectra, the sample H showed the highest absorbance in the range of 1600-1550 $cm^{-1}$ and 1450-1400 $cm^{-1}$, which corresponded to level of the acetyl group in glucomannan. The sample H of freeze-dried powder was the highest in polysaccharide content, jack bean urease inhibition rate, and FAC (fat adsorption capacity). It could be inferred that the sample H had an effect on Helicobacter pylori inhibition and fat adsorption suppression according to urease inhibition rate and FAC, respectively.

Konjac Glucomannan Derived Carbon Aerogels for Multifunctional Applications

  • Lian, Jie;Li, Jiwei;Wang, Liang;Cheng, Ru;Tian, Xiuquan;Li, Xue;Zhou, Jian;Duan, Tao;Zhu, Wenkun
    • Nano
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    • v.13 no.10
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    • pp.1850113.1-1850113.11
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    • 2018
  • Environmental and energy issues have always been a hot topic of global research. Oil leakage has caused great damage to the environment, affecting a wide area and it is difficult to clean up. In most cases, carbon-based adsorbents are typically utilized to remove oil spills because of their economic benefits and high adsorbent efficiency. At the same time, its excellent material properties can also be used for the preparation of supercapacitors. In this paper, the carbon aerogels were prepared by the one-step method. The prepared materials endowed a 3D network structure with a huge number of micropores and mesoporous, and the material is light-weight, stable, hydrophobic and has affinity for oil (17.02 g/g) to the KGM carbon aerogel. Through the physicchemical characterization, the KGM carbon aerogel shows specific surface area is $689m^2/g$, high water contact angle ($136.64^{\circ}$) and excellent reusability (more than 15 cycle times). In addition, we also discussed the electrochemical properties of the material and obtained the specific electrical capacity of 139 F/g under the condition of 1 A/g.

Medications for Child with Chronic Constipation (소아 변비 치료에서 약물사용)

  • Bae, Sun Hwan
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.12 no.sup1
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    • pp.111-117
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    • 2009
  • Constipation is one of the most common symptoms that a child visits pediatrician. The general approach to the child with functional constipation includes the following steps; disimpaction, maintenance and withdrawal of medication. There are many drugs which can be applied to children; osmotic agents (lactulose, sorbitol, magnesium hydroxide/citrate, polyethylene glycol with/without electrolytes, sodium phosphate, glycerin), stimulants (senna, bisacodyl, caster oil), lubricant (mineral oil), bulking agent (psyllium, cellulose, glucomannan). At each stage of treatment, one or some of these drug can be applied to the purpose. The author tries to summarize recent studies on drugs for constipation in child, and finally introduces new dugs for constipation which is under investigation.

Yellowing of Chemical Pulp by Glucuronoxylan

  • Kim, Young-Seok;Yoon, Byung-Ho
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.38 no.3 s.116
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    • pp.23-28
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    • 2006
  • Xylan in hemicellulose has been reported as one of the reasons of the yellowing of chemical pulp and paper. But little relevant information is available in literature. In this study, we examined into the influence that glucuronoxylan or glucomannan and metallic ion($Cu^{2+},\;Fe^{2+},\;Fe^{3+},\;Mn^{2+}$) residued in pulp get each or when mixed at yellowing. Filter paper was treated with each metallic ion, glucuronoxylan and the mixture of glucuronoxylan and metal ions, and brightness and CIE $L^*a^*b^*$ was measured after accelerated aging. As the results of measurements, the filter paper processed by 10% glucuronoxylan was dropped $2{\sim}3%$ on the brightness after accelerated aging for 24 hours. Also, the filter paper treated with glucuronoxylan and $Fe^{2+}$ was dropped 7% on the brightness.

Yellowing of Chemical Pulp by Adding Glucuronoxylan

  • Yoon, Byung-Ho;Kim, Young-Seok
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2006.06b
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    • pp.361-364
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    • 2006
  • Generally, aging of paper is evaluated by folding endurance and degree of yellowing. It was known as that lignin, hemicellulose and metallic ion affect yellowing of mechanical or chemical pulps. Among these elements, especially, the study of hemicellulose that affect at yellowing of pulp is insufficient. In this study, we examined into the influence that glucuronoxylan or glucomannan and metallic ion($Cu^{2+},\;Fe^{2+},\;Fe^{3+},Mn^{2+}$) residued in pulp get each or when mixed at yellowing. Filter paper was treated with each metallic ion, glucuronoxylan and the mixture of glucuronoxylan and metal ions, and brightness and CIE $L^*a^*b^*$ was measured after accelerated aging. As the results of measurements, the filter paper processed by 10% glucuronoxylan was dropped 2-3% on the brightness after accelerated aging for 24 hours. Also, the filter paper treated with glucuronoxylan and $Fe^{2+}$ was dropped 7% on the brightness.

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Cloning a Mannanase 26AT Gene from Paenibacillus woosongensis and Characterization of the Gene Product (Paenibacillus woosongensis으로부터 Mannanase 26AT 유전자의 클로닝과 유전자 산물의 분석)

  • Yoon, Ki-Hong
    • Journal of Life Science
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    • v.27 no.9
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    • pp.1003-1010
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    • 2017
  • An open reading frame coding for mannanase predicted from the partial genomic sequence of Paenibacillus woosongensis was cloned into Escherichia coli by polymerase chain reaction amplification, and completely sequenced. This mannanase gene, designated man26AT, consisted of 3,162 nucleotides encoding a polypeptide of 1,053 amino acid residues. Based on the deduced amino acid sequence, Man26AT was identified as a modular enzyme, which included a catalytic domain belonging to the glycosyl hydrolase family 26 and two carbohydrate-binding modules, CBM27 and CBM11. The amino acid sequence of Man26AT was homologous to that of several putative mannanases, with identity of 81% for P. ihumii and identity of less than 57% for other strains of Paenibacillus. A cell-free extract of recombinant E. coli carrying the man26AT gene showed maximal mannanase activity at $55^{\circ}C$ and pH 5.5. The enzyme retained above 80% of maximal activity after preincubation for 1 h at $50^{\circ}C$. Man26AT was comparably active on locust bean gum (LBG), galactomanan, and kojac glucomannan, whereas it did not exhibit activity on carboxymethylcellulose, xylan, or para-nitrophenyl-${\beta}$-mannopyranoside. The common end products liberated from mannooligosaccharides, including mannotriose, mannotetraose, mannopentaose, and mannohexaose, or LBG by Man26AT were mannose, mannobiose, and mannotriose. Mannooligosacchrides larger than mannotriose were found in enzymatic hydrolyzates of LBG and guar gum, respectively. However, Man26AT was unable to hydrolyze mannobiose. Man26AT was intracellularly degraded into at least three active proteins with different molecular masses by zymogram.