• 제목/요약/키워드: Ginseng leaves

검색결과 356건 처리시간 0.019초

편평세포암 동물 모델에서 고려인삼잎-다당체(MB40)의 항암치료효과 (The Anti-tumor Effect of Polysaccharide from the Leaves of Panax Ginseng C.A. Meyer(MB40) in a Murine Squamous Cell Carcinoma Model)

  • 주은정;최준;정은재;홍석진;조재구;백승국;우정수;정광윤;권순영
    • 대한두경부종양학회지
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    • 제25권1호
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    • pp.3-7
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    • 2009
  • Background and Objectives : Panax ginseng C.A. Meyer is a medical plant that has been widely utilized as a tonic and nutritional agent since ancient times in Korea. Ginseng has anti-metastatic property of cancer and immunomodulating activity. The novel acidic polysaccharide compound(MB40) was isolated from the leaves of Panax ginseng C.A. Meyer. To determine immunomodulating activities of MB40, we evaluate anti-cancer and anti-metastatic effects of MB40 in tumor bearing immune competent mice. Material and Methods : C3H mice were divided into three equal groups(Cisplatin treatment group, MB40 treat-ment group, Cisplatin and MB40 treatment group) and were transplanted SCC(Squamous Cell Carcinoma) cells(2${\times}$106) to the lateral side of abdomen. From day 4 after transplantation, MB40 was administrated at dose of 10mg/kg, respectively, every other day by intratumoral injection. Cisplatin was systemically administrated at doses of 1mg/kg, respectively, every week by intraperitoneal injection. Results : 5 days after administration, tumors can be palpated in every mice group. After 13 days of administration, the mice group to which MB40 were administrated exhibited reduction in tumor size respectively, compared to cisplatin group. Overall status of mice such as body weight and activity were superior in MB40 group than cisplatin group. Conclusion : The result of this study indicates MB40 may have significant therapeutic effect and decreases complications induced by systemic chemotheraphy. MB40 may be developed as a novel and potent immunotropics to improve the cell immune system and anti-cancer drug for the treatment of cancer patients in head and neck squamous cell carcinoma.

Cosmetic Potential of Enzymatic Treated Ginseng Leaf

  • Lee, Hyun-Sun;Lee, Hyun-Jung;Cho, Hye-Jin;Park, Sung-Sun;Kim, Jin-Man;Suh, Hyung-Joo
    • Journal of Ginseng Research
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    • 제34권3호
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    • pp.227-236
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    • 2010
  • The objective of this study was to evaluate the potential use of ginseng leaf as a cosmetic material. In this research, we employed enzymatic treated ginseng leaf by using Ultraflo L to improve the recovery of ginsenosides from the ginseng leaf and studied the biological activities and skin safety of the enzymatic treated ginseng leaf for use as a cosmetic material. The total ginsenoside contents of the non-enzymatic treated ginseng leaf (NEGL) and Ultraflo L treated ginseng leaf (UTGL) were 271 and 406 mg/g, respectively. The level of metabolite ginsenosides (sum of Rg2, Rg3, Rg5, Rk1, compound K, Rh1, Rh2, and F2) was higher in UTGL (93.1 mg) compared to NEGL (62.4 mg) in one gram ginseng leaf extract. The increase in amounts of ginsenoside types in UTGL compared to NEGL was generally 140% to 157%. UTGL exhibited relatively higher 2,2-diphenyl-2-picrylhydrazyl hydrate ($IC_{50}$, 2.8 mg/mL) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt ($IC_{50}$, 1.6 mg/mL) radical scavenging activities compared to NEGL (4.8 mg/mL and 2.2 mg/mL). The UTGL group showed normalized hydrogen peroxide, lipid peroxidation and visual wrinkling grade induced-UVB exposure. The UTGL did not induce any adverse reactions such as erythema and edema on intact skin sites; however, some guinea pigs treated with UTGL on abraded skin sites showed very slight erythema. The primary irritation index (PII) score of UTGL was 0.05 and it was classified as a practically non-irritating material (PII, 0 to 0.5). In skin sensitization tests with guinea pigs, UTGL had a positive rate of skin sensitization at 40%, and the mean evaluation score was 0.4.

Cytohistological study of the leaf structures of Panax ginseng Meyer and Panax quinquefolius L.

  • Lee, Ok Ran;Nguyen, Ngoc Quy;Lee, Kwang Ho;Kim, Young Chang;Seo, Jiho
    • Journal of Ginseng Research
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    • 제41권4호
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    • pp.463-468
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    • 2017
  • Background: Both Panax ginseng Meyer and Panax quinquefolius are obligate shade-loving plants whose natural habitats are broadleaved forests of Eastern Asia and North America. Panax species are easily damaged by photoinhibition when they are exposed to high temperatures or insufficient shade. In this study, a cytohistological study of the leaf structures of two of the most well-known Panax species was performed to better understand the physiological processes that limit photosynthesis. Methods: Leaves of ginseng plants grown in soil and hydroponic culture were sectioned for analysis. Leaf structures of both Panax species were observed using a light microscope, scanning electron microscope, and transmission electron microscope. Results: The mesostructure of both P. ginseng and P. quinquefolius frequently had one layer of non-cylindrical palisade cells and three or four layers of spongy parenchymal cells. P. quinquefolius contained a similar number of stomata in the abaxial leaf surface but more tightly appressed enlarged grana stacks than P. ginseng contained. The adaxial surface of the epidermis in P. quinquefolius showed cuticle ridges with a pattern similar to that of P. ginseng. Conclusion: The anatomical leaf structure of both P. ginseng and P. quinquefolius shows that they are typical shade-loving sciophytes. Slight differences in chloroplast structure suggests that the two different species can be authenticated using transmission electron microscopy images, and light-resistant cultivar breeding can be performed via controlling photosynthesis efficiency.

The Panax ginseng Flowering Locus T Shows Age Specific Expression Pattern in Ginseng and Increases Root Length in Transgenic Arabidopsis

  • Mohanan, Padmanaban;Myagmarav, Davajargal;Zhang, Dabing;Kim, Yu-Jin;Yang, Deok-Chun
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2018년도 춘계학술발표회
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    • pp.17-17
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    • 2018
  • Panax ginseng Meyer is a perennial medicinal plant, the roots of which has been used in the traditional formulations in Oriental countries. To understand its floral transition, we isolated Flowering Locus T (FT) from ginseng, the bioinformatics analysis of PgFT has revealed a considerable homology to the higher plants, with the essential amino acids for FT function are conserved. The phylogenetic analysis has shown that the PgFT is belonged to the shrub classes of plants and closest kin to Jatropha curcas FT. The expression profiling from juvenile (2-year-old) were abundant in leaves as well as in root and was concentrated in the secondary leaflet and stem bottom in adult (4-year-old) ginseng plant tissues, moreover PgFT transcript displayed photoperiod dependent oscillation. The ectopic expression of PgFT in Arabidopsis thaliana, exhibit precocious flowering and several floral pathway integrators were up-regulated, interestingly their root length was increased in the transgenic seedlings. Therefore, we could conclude that PgFT encodes a florigen that acts as a key regulator in the flowering pathway in ginseng and hypothesize that, it might involve in the underground organ development as well. We believe our finding could provoke future studies on the physiology and development in P. ginseng.

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고온(高溫)과 재배광도(栽培光度)가 인삼(人蔘) 잎의 지방산(脂肪酸) 조성(組成)에 미치는 영향(影響) (Effect of High Temperature and Growth Light Intensity on Fatty Acid Composition of Panax ginseng leaf)

  • 박훈;박현석;홍종욱
    • Applied Biological Chemistry
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    • 제29권4호
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    • pp.366-371
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    • 1986
  • 광도(光度)가 다른 포장조건에서 자란 6년근 인삼잎과 온도별 20시간 처리된 관행재배 6년근 절제엽중(切除葉中)의 지방산을 gas chromatography로 분석하였다. 재배광도(온도)가 높을수록 지방산 함량이 감소하였다. Linoleic, linolenic, palmitic과 palmitoleic acid가 다량(多量)지방산으로 80%였다. 지방산 조성의 유사도는 광도 20%까지는 차이가 크지 않았으나 30%에서 차이를 보여 20%가 재배 한계 광도인 것 같다. 재배광도가 높을수록 불포화 지방산비율이나 불포화대(不飽和帶)의 비율이 적어져서 고온에 대한 적응 기작으로 보였다. 30% 수광율과 수확기잎이 지방산특성이 유사하였으며 고온적응과 노화(老化)의 관계가 불분명(不分明)하였다. 단시간(短時間)의 고온처리 ($25^{\circ}$$35^{\circ}C$)는 지방산함량, 불포화산비율 및 불포화도의 비율을 증대시켰고 지방산 조성이 유사하여 장기 온도효과와는 반대의 결과였다.

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Comparison of Lines From Anther and Maternally-derived Dihaploids in Flue-cured Tobacco(Nicotiana tabacum L.)

  • Chung, Yun-Hwa;Keum, Wan-Soo;Choi, Sang-Ju;Cho, Myung-Cho
    • 한국연초학회지
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    • 제21권1호
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    • pp.82-88
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    • 1999
  • The present study was conducted to compare the relative efficiency of two different haploid breeding methods in tobacco varietal development. A single FI hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L., Bright Yellow4(BY4) and NC95, was used to develop the 30 anther derived dihaploid (ADH) lines and maternally-derived dihaploid (MDH) lines utilizing Nicotiana africana, respectively. As compared with mid-parent, mean of ADH lines showed increase in number of leaves, delay in days to flower and narrow in width of leaf. However, no significant differences in the other investigated characters were recognized. MDH lines also showed narrow width of leaf, while no significant differences in the other characters were observed. The variations of the investigated characters were generally greater in ADH than MDH lines. MDH lines had higher height of plant and shorter days to flower than ADH lines, while the other characters did not show remarkable differences. The degree of heritability for each of the characters observed between ADH and MDH was almost the same. The characters showing high heritability value were height of plant, number of leaves, days to flower, and yield, while those showing relatively low value were length of leaf, width of leaf, and total alkaloid content. Predicted gains from selection for increased yield were calculated for both populations(ADH, MDH) and correlated responses associated with selection for yield were estimated. Height of plant, width of leaf, days to flower, and concentration of reduceing sugar would be expected to improve with selection for yield much faster in the MDH population than in the ADH.

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DNA marker를 이용한 풍기 재래인삼의 주요 특성 분석 (Analysis of Major Traits for Native Ginseng(Panax ginseng C.A. Meyer) Collected from Poonggi Area in Korea Using DNA Marker)

  • 임순영;최홍집;류태석;권태룡;최진국;손재근
    • 한국작물학회지
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    • 제55권3호
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    • pp.253-258
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    • 2010
  • 국내 수집 재래종 인삼과 기존육성품종과의 유전적 연관을 통해 풍기지역 고유의 재래인삼 특성을 구명하고 지리적 표시제 및 신품종 육성을 위한 자료로 활용하기 위하여 수행한 결과는 다음과 같다. 유전적 연관 분석에서 가장 먼 것으로 분류된 풍기지역 수집종 331002 등 5점과 금산지역 수집종 332009 등 5점간의 형태적 특성을 비교한바 풍기 재래종 인삼의 경수는 1~2개로 금산지역 수집종과 차이가 없었으나, 장엽수, 경색, 엽병색, 소엽 모양에서는 금산지역 수집종과 차이를 보였다. 즉 풍기 재래종은 장엽수가 5개이고 소엽 모양이 타원형이며, 경색과 엽병색이 자색, 연자색, 진자색 등 다양한 것이 특징적이었다. 특히 유전적 연관에서 풍기지역 수집종 중 331002, 331005, 331007 및 331026은 함께 grouping 되었지만 331002는 대비 품종인 천풍과 99.5%에서 함께 group지어져 있었다. 특히 331002 등 이 3개의 풍기 수집종은 기존 품종과 유전적 차이를 나타내보였다. 이를 통해 풍기 수집종 331002, 331005, 331007은 국내 재래종에서 순계분리법으로 선발된 기존육성품종과는 유전적 차이가 있음을 알 수 있었다. 특히 331007은 농가 조사에서 나타난 주요 특성을 모두 갖추고 있어 풍기 재래인삼의 고유 특성을 가진 것으로 추측 되었다. 본 연구를 통해 풍기지역 재래 인삼의 주요 형태적 특성을 확인하였고, 유전적인 면에서도 타 지역 수집종과 기존육성품종과도 구분되어짐을 확인하였다. 더불어 선발된 OPD05 등 9개의 prime는 경수 1개, 장엽수 5개, 소엽 모양은 타원형, 경색과 엽병색이 자색계열인 특성을 지닌 풍기 재래인삼을 구분하는데 유용하게 활용될 수 있어서 인삼 유전자원의 특성 구분 및 품종개량에 유익하게 활용될 수 있을 것으로 사료된다.

새싹인삼 추출물의 항산화 활성 및 MMP-1 저해 활성 (Antioxidant activity and MMP-1 inhibitory activity of Panax Ginseng Sprout Extracts)

  • 김민정;양예진;양주혜;이원융;김우현;이재남;박광일
    • 대한한의학방제학회지
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    • 제32권1호
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    • pp.83-90
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    • 2024
  • Objectives : As a substitute for high-price ginseng, this study attempted to examine a possibility of the ferment extract of Panax ginseng sprout whether leaves and roots can be used together as a cosmetic ingredient with anti-oxidative and wrinkle-care effects. Methods : In terms of a test method, antioxidant activities were confirmed through total polyphenol contents, total flavonoid contents, DPPH radical scavenging activity and ABTS radical scavenging activity using the Panax ginseng sprout. In addition, to assess wrinkle-care effectiveness, the cytotoxicity of the extract was analyzed through MTT assay, and inhibition of collagenase activity in the cells was tested using the Panax ginseng sprout fermented by Saccharomyces cerevisiae. Resuits : The content of polyphenols and flavonoids in natural plants was highest in Panax Ginseng Sprout Extract at 100℃, which also demonstrated high DPPH, ABTS radical scavenging activity. MTT assay demonstrated that the Panax Ginseng Sprout Ferment Extract did not have a cytotoxic effect in CCD-986SK cell. Also, Panax Ginseng Sprout Ferment Extract was found to inhibit MMP-1 expression by 51.85±6.09% at a concentration of 10%. Conclusions : Therefore, this study has confirmed a possibility of Panax ginseng sprout ferment extract as a cosmetic ingredient with MMP-1-inhibitory effects.

한국 및 중국산 산양삼의 중금속 오염도 측정

  • 김종윤;김병우;권기록
    • 대한약침학회지
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    • 제10권1호통권22호
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    • pp.67-77
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    • 2007
  • Objectives : Heavy metal contents in cultivated wild ginseng from Korea and China were evaluated for the safety purpose. Methods : Arsenic(As), cadmium(Cd), lead(Pb), mercury(Hg), copper(Cu), chromium(Cr), and selenium(Se) from the root, stem, and leaves of the Korean cultivated wild ginseng and the root of the Chinese cultivated wild ginseng were analyzed for the existence of heavy metal contents. Results : Most of heavy metals weren't detected in all the samples. Copper and lead were found in very minute volume and didn't reach the toxic concentration level of 10mg/kg. Samples used in the test were grown in non-contaminated areas, free from heavy metal contamination. Conclusions : Based on above results, cultivated wild ginseng from Korea and China are relatively safe from the heavy metal exposure. But for more rigorous quality control, an epidemiological survey using the samples from more diverse areas should be conducted.

토양 염류 농도가 인삼 잎의 엽록소 형광반응 및 생리장해 발생에 미치는 영향 (Effects of Salt in Soil Condition on Chlorophyll Fluorescence and Physiological Disorder in Panax ginseng C. A. Meyer)

  • 김장욱;현동윤;김영창;이정우;조익현;김동휘;김기홍;손재근
    • 한국약용작물학회지
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    • 제23권6호
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    • pp.446-453
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    • 2015
  • Background : Excessively high concentration of sodium ion causednutrient deficiency and significantly decrease growth. This study was carried out to determine the limiting concentration range of sodium ion in the soil of ginseng field. Methods and Results : The growth of the ginseng cultivar Chunpoong reduced with increase in salinity, and the rate of growth reduction was higher in shoots than that of roots. Particularly, ginseng plants cultivated at high level of nitrate nitrogen or sodium may suffer delayed development and stunted growth. Chlorophyll damage occurred on the leaves of ginseng planted in relatively high levels (> $0.2cmol^+/kg$) of sodium ion, as determined by the fluorescence reaction. The incidence of physiological disorder in ginseng cultivated at 249 sites was correlated with the concentration of sodium ion in the soils. About 74% of ginseng fields in which physiological disorders occurred had concentrations of sodium ion in soil greater than $0.2cmol^+/kg$. In contrast, the concentration of sodium ions at 51 of 85 sites where no damage occurred was relatively ($0.05cmol^+/kg-0.15cmol^+/kg$). Conclusions : The concentration of sodium ion in soil of ginseng fields can be classified into three levels optimum (${\leq}0.15$), permissible allowance (0.15 - 0.2) and excessive (> 0.2).