• Journal of Ginseng Research
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• v.40 no.1
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• pp.68-75
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• 2016

Background: The study of phenolic compounds profiles and antioxidative activity in ginseng fruit, leaves, and roots with respect to cultivation years, and has been little reported to date. Hence, this study examined the phenolic compounds profiles and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free-radical-scavenging activities in the fruit, leaves, and roots of Korean ginseng (Panax ginseng Meyer) as a function of cultivation year. Methods: Profiling of 23 phenolic compounds in ginseng fruit, leaves, and roots was investigated using ultra-high performance liquid chromatography with the external calibration method. Antioxidative activity of ginseng fruit, leaves, and roots were evaluated using the method of DPPH free-radical-scavenging activity. Results: The total phenol content in ginseng fruit and leaves was higher than in ginseng roots (p < 0.05), and the phenol content in the ginseng samples was significantly correlated to the DPPH free-radical-scavenging activity ($r=0.928^{****}$). In particular, p-coumaric acid ($r=0.847^{****}$) and ferulic acid ($r=0.742^{****}$) greatly affected the DPPH activity. Among the 23 phenolic compounds studied, phenolic acids were more abundant in ginseng fruit, leaves, and roots than the flavonoids and other compounds (p < 0.05). In particular, chlorogenic acid, gentisic acid, p- and m-coumaric acid, and rutin were the major phenolic compounds in 3e6-yr-old ginseng fruit, leaves, and roots. Conclusion: This study provides basic information about the antioxidative activity and phenolic compounds profiles in fruit, leaves, and roots of Korean ginseng with cultivation years. This information is potentially useful to ginseng growers and industries involved in the production of high-quality and nutritional ginseng products.

• Journal of Ginseng Research
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• v.36 no.4
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• pp.425-429
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• 2012

In this study, hydroponically-cultivated ginseng leaves, fruits, and roots were respectively extracted with ethanol. The contents of 12 ginsenosides and three phenolics in the extracts were quantitatively analyzed and the free radical scavenging activities were measured and compared. Hydroponically-cultivated ginseng leaves contained higher levels of gensenosides (Rg1, Rg2+Rh1, Rd, and Rg3) and p-coumaric acid than the other parts of the ginseng plants. The 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid radical scavenging activities of leaves were also the highest. Accordingly, hydroponically-grown ginseng leaves were shown to hold promise for use as an environmentally-friendly natural anti-oxidant.

• Preventive Nutrition and Food Science
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• v.16 no.4
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• pp.321-327
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• 2011

Water, methanol and ethanol extracts of ginseng leaves were assayed for total phenolics and flavonoids, ascorbic acid, cupric and ferrous ion chelating activities, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP) assay and ABTS radical cation decolourization (TEAC) assay for their antioxidant properties. The ethanol extract of ginseng leaves contained significantly (p<0.05) higher amounts of total phenolics and flavonoids (600.57 and 1701 mg/100 g) than methanol (374.43 and 1512.64 mg/100 g) and water extracts (248.30 and 680.05 mg/100 g). Among solvent extracts of ginseng leaves, the ethanol extract showed the most powerful antioxidant activities. However, the ferrous ion chelating activity of ginseng leaf extracts were lower than the cupric ion chelating ability. These differences in concentrations of key antioxidants among various solvent extracts seemed to be responsible for their differences in antioxidant activities. These results suggest that ethanol extract of ginseng leaves has the most effective antioxidant capacity compared to the methanol and water extracts tested in the present study. Thus, it can be applied for the effective extraction of functional material from ginseng leaves for the usage of pharmaceutical and/or food industries.

• Journal of Ginseng Research
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• v.34 no.1
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• pp.68-75
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• 2010

This study sought to optimize the extraction and enzymatic treatment conditions of Panax ginseng leaves, stems, and roots for the production of fermented ginseng. The optimization enhanced the extraction of total saccharide, a nutrient and growth-activating factor for Lactobacillus bacteria. The hydrolysis of ginseng leaves, stems, and roots was tested with eight enzymes (Pentopan, Promozyme, Celluclast, Ultraflo, Pectinex, Ceremix, Viscozyme, and Tunicase). The enzymatic hydrolysis conditions were statistically optimized by the experimental design. Optimal particle size of ginseng raw material was <0.15 mm, and optimal hydrolysis occurred at a pH of 5.0-5.5, a reaction temperature of 55-$60^{\circ}C$, a Ceremix concentration of 1%, and a reaction time of 2 hr. Ceremix produced the highest dry matter yield and total saccharide extraction. Ginseng leaves were found to be the most suitable raw material for the production of fermented ginseng because they have higher carbohydrate and crude saponin contents than ginseng roots.

• Journal of Ginseng Research
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• v.15 no.2
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• pp.120-123
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• 1991

Flavor components of panax ginseng cultured with pine tree leaves mulch instead of traditional rice straw were examined. The growth of two year old ginsengs grown with two different kinds of mulchs no difference, however, the flavor components of ginseng with pine tree leaves mulch 84 constituents detected showed significantly enhanced contents than those of ginseng with rice straw mulch.

• Journal of Ginseng Research
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• v.12 no.1
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• pp.11-29
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• 1988

This study was conducted to investigate the effect of light intensity and temperature on the photosynthesis and respiration of ginseng plant. Highly significant, second degree curvilinear regressions were recognized among the photosynthesis of ginseng leaves, light intensity and temperature. And an interaction between the effects of light intensity and temperature on the photosynthesis of ginseng leaves was found to be highly significant. The increasing rate of photosynthesis with the increase of light intensity was markedly decreased with increasing temperature. The light compensation point of ginseng leaves was significantly varied with temperature, and the average point was approximately 600 lux. The light saturation point of Korean ginseng was 11,000 lux at $15^{\circ}C$ and $20^{\circ}C$ and around 9,500 lux at above $25^{\circ}C$. The decreasing rate of photosynthesis with the increase of temperature significantly increased with increasing light intensity. The optimum temperature for the photosynthesis of ginseng leaves was about 15 to $22^{\circ}C$ and markedly decreased with increasing light intensity. The highest photosynthesis occurred in ginseng leaves grown with the shade of 15% transmittance. The respiration of ginseng leaves increased with the shade of 5% and/or 30% transmittance. High temperature stimulated the respiration of ginseng leaves. Percent respiration to photosynthesis of ginseng leaves grown with the shade was increased at high temperature and decreased with increasing light Intensity. It was also increased with increasing transmittance. The maximum $CO_2$ absorption of ginseng leaves grown with the shade of 5Ps and ISVS transmittance accurred at 9 o'clock a.m., whereas that of 20% transmittance occurred at 7-9 o'clock a.m. The duration of $CO_2$ absorption was distinctively long with the shade of high transmittance. The $CO_2$ compensation point in the photosynthesis of ginseng leaves was 130 ppm.

• Journal of Ginseng Research
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• v.45 no.1
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• pp.163-175
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• 2021

Background: Ginsenosides, which have strong biological activities, can be divided into polar or less-polar ginsenosides. Methods: This study evaluated the phytochemical diversity of the saponins in Panax ginseng (PG) root, American ginseng (AG) root, and Panax notoginseng (NG) root; the stem-leaves from Panax ginseng (SPG) root, American ginseng (SAG) root, and Panax notoginseng (SNG) root as well as the saponins obtained following heating and acidification [transformed Panax ginseng (TPG), transformed American ginseng (TAG), transformed Panax notoginseng (TNG), transformed stem-leaves from Panax ginseng (TSPG), transformed stem-leaves from American ginseng (TSAG), and transformed stem-leaves from Panax notoginseng (TSNG)]. The diversity was determined through the simultaneous quantification of the 16 major ginsenosides. Results: The content of ginsenosides in NG was found to be higher than those in AG and PG, and the content in SPG was greater than those in SNG and SAG. After transformation, the contents of polar ginsenosides in the raw saponins decreased, and contents of less-polar compounds increased. TNG had the highest levels of ginsenosides, which is consistent with the transformation of ginseng root. The contents of saponins in the stem-leaves were higher than those in the roots. The transformation rate of SNG was higher than those of the other samples, and the loss ratios of total ginsenosides from NG (6%) and SNG (4%) were the lowest among the tested materials. In addition to the conversion temperature, time, and pH, the crude protein content also affects the conversion to rare saponins. The proteins in Panax notoginseng allowed the highest conversion rate. Conclusion: Thus, the industrial preparation of less-polar ginsenosides from SNG is more efficient and cheaper.

• Journal of Ginseng Research
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• v.14 no.1
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• pp.6-9
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• 1990

Specific activities of ADC and ODC from 2-4 year old ones were higher than that from seedlings whereas those activities were not changed significantly from 2 to 4 years. Generally, activity of ADC was predorminant compared to that of ODC. Free arginine content in roots was much higher than that of leaves. And arginase specific activity from roots was higher than that of leaves. Cumulative results suggest that putrscine formation from ornithine in roots may be more effective than leaves and contribute to putrescine biosynthesis to some extract.

• Journal of Ginseng Research
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• v.16 no.1
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• pp.1-6
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• 1992

Chemical composition was determined to evaluate the quality of Panel ginseng-leaf tea over green teas. Ginseng-leaf tea was shown to contain higher contents of soluble matter, ascorbic acid and lower contents of tannins, as compared to tea leaves. The profiles of ginsenoside and sugar of ginseng-leaf tea were noticeably different from those of ginseng roots and the sample maintained high levels of these components under the manufacturing process. Total unsaturated fatty acids and free amino acids were estimated to be decreased in ginseng-leaf tea as compared to those of ginseng leaves. The compositions of amino acids and minerals in ginseng-leaf tea were similar to those of tea leaves and glutamic acid, aspartic acid, leucine, calcium, potassium, sodium, and copper were found to be major components.

• Microbiology and Biotechnology Letters
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• v.9 no.3
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• pp.129-137
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• 1981

The purpose of this study was to extract saponin efficiently from ginseng leaves and peelings by macerating them with microbial enzyme. To begin with, we selected G-211 strain having the highest macerating activity among several rotting molds of fresh ginseng. Crude macerating enzyme was prepared from this G-2l1 strain by ammonium sulfate precipitation, and was applied to macerating leaves and peelings of ginseng. The optimal pH of the enzyme for maceration was 5.0 in both leaves and peelings of ginsen g. The optimal pH for the extraction of soluble matters and saponins was 4.5 and 5.5 in ginseng leaves and ginseng peelings, respectively. When this enzyme was treated together with crude cellulase from Trichoderma viride (To4), the extract content of saponin was 3.45% for ginseng leaves and 3.90% for ginseng peelings. Their yields were 39.8 % and 39.3 % of total saponin amounts in ginseng leaves and ginseng peelings, respectively. The ginsenoside patterns of saponins extracted with the treatment of enzymes were also studied by HPLC technics.