Extraction of Ginseng Saponin by the Treatment of Microbial Macerating Enzyme

미생물이 생성한 식물조직부양효소를 이용한 인삼 Saponin의 추출

The purpose of this study was to extract saponin efficiently from ginseng leaves and peelings by macerating them with microbial enzyme. To begin with, we selected G-211 strain having the highest macerating activity among several rotting molds of fresh ginseng. Crude macerating enzyme was prepared from this G-2l1 strain by ammonium sulfate precipitation, and was applied to macerating leaves and peelings of ginseng. The optimal pH of the enzyme for maceration was 5.0 in both leaves and peelings of ginsen g. The optimal pH for the extraction of soluble matters and saponins was 4.5 and 5.5 in ginseng leaves and ginseng peelings, respectively. When this enzyme was treated together with crude cellulase from Trichoderma viride (To4), the extract content of saponin was 3.45% for ginseng leaves and 3.90% for ginseng peelings. Their yields were 39.8 % and 39.3 % of total saponin amounts in ginseng leaves and ginseng peelings, respectively. The ginsenoside patterns of saponins extracted with the treatment of enzymes were also studied by HPLC technics.

인삼엽 및 인삼피를 식물조직부양효소 (macerating enzyme)로 분리시킴으로써 인삼의 유효성분인 saponin을 효율적으로 추출하고자 인삼부패균중 조직부양력이 강력한 1종의 G-211 균주를 선정하여 조제한 조효소로써 실험하였다. 조직부양작용의 최적 pH 는 인삼엽, 인삼피 공히 pH 5.0이었으며 가용성물질추출율 및 saponin추출율은 인삼엽이 pH4.5, 인삼피가 pH5.5에서 가장 효과가 컸다. 조직부양효소와 조 cellulase을 qudydd처리하므로써 인삼엽, 인삼피 각각 3.45%, 3.90%의 saponin을 추출할 수 있었는데 이 추출율은 전체 saponin 함량의 39.8%, 39.3%에 해당되는 수율이었다. 또한 추출된 saponin을 HPLC를 이용해서 각 ginsenoside별 pattern도 조사하였다.