• Journal of Plant Biology
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• 제32권4호
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• pp.255-264
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• 1989

Ginseng zygotic embryos, seedlings, and exised cotyledonary nodes were cultured on Murashinge and Skoog's(MS) medium, supplemented with 6-benzyladenine(BA) and gibberellic acid(GA3) to induce flower buds. As the concenteration of nitrogen compounds in MS medium was reduced to half of its strength, the flowering frequency of zygotic embryos increased up to 90%. The optimum concentration of sucrose in the medium for flowering of seedlings was 30-60 g/1. In all cases flower buds were formed on elongated axillary branches from the cotyledonary nodes, while the apices remained vegetative. When zygotic embryos and excised cotyledonary nodes were cultured on the medium, supplemented with all possible combinations of BA, GA3, and abscisic acid(ABA) of 5 $\mu$M indole-3-acetic acid(IAA) in the above combinations did not affect flowering. These results suggest that cytokinins, gibberellins, and inhibitors play primary, permissive, and preventive roles, respectively, in the induction of flowering of ginseng.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2006년도 제47회 학술심포지움 및 추계국제학술대회
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• pp.116.1-116.1
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• 2006

• Applied Biological Chemistry
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• 제28권2호
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• pp.82-87
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• 1985

GC-SIM분석법에 의한 GA의 동정 및 정량분석에 대한 정확성과 정출감도의 향상을 목적으로, 29종의 GA를 Me-TMS와 TMS-TMS유도체를 만들고, GC-MS에 의해 이들 유도체의 mass spectra를 측정하였으며, GC-SIM분석에 있어서 Me-TMS유도체와 TMS-TMS유도체의 장단점에 대해 검토하였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2001년도 춘계 학술대회지
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• pp.50-51
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• 2001

• The Plant Pathology Journal
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• 제40권2호
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• pp.99-105
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• 2024

Land plants produce glucose (C₆H₁₂O₂) through photosynthesis by utilizing carbon dioxide (CO₂), water (H₂O), and light energy. Glucose can be stored in various polysaccharide forms for later use (e.g., sucrose in fruit, amylose in plastids), used to create cellulose, the primary structural component of cell walls, and immediately metabolized to generate cellular energy, adenosine triphosphate, through a series of respiratory pathways including glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation. Additionally, plants must metabolize glucose into amino acids, nucleotides, and various plant hormones, which are crucial for regulating many aspects of plant physiology. This review will summarize the biosynthesis of different plant hormones, such as auxin, salicylic acid, gibberellins, cytokinins, ethylene, and abscisic acid, in relation to glucose metabolism.

• Journal of Microbiology and Biotechnology
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• 제25권9호
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• pp.1467-1475
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• 2015

The use of microbial extracts containing plant hormones is a promising technique to improve crop growth. Little is known about the effect of bacterial cell-free extracts on plant growth promotion. This study, based on phytohormonal analyses, aimed at exploring the potential mechanisms by which Enterococcus faecium LKE12 enhances plant growth in oriental melon. A bacterial strain, LKE12, was isolated from soil, and further identified as E. faecium by 16S rDNA sequencing and phylogenetic analysis. The plant growth-promoting ability of an LKE12 bacterial culture was tested in a gibberellin (GA)-deficient rice dwarf mutant (waito-C) and a normal GA biosynthesis rice cultivar (Hwayongbyeo). E. faecium LKE12 significantly improved the length and biomass of rice shoots in both normal and dwarf cultivars through the secretion of an array of gibberellins (GA₁, GA₃, GA₇, GA₈, GA₉, GA₁₂, GA₁₉, GA₂₀, GA₂₄, and GA₅₃), as well as indole-3-acetic acid (IAA). To the best of our knowledge, this is the first study indicating that E. faecium can produce GAs. Increases in shoot and root lengths, plant fresh weight, and chlorophyll content promoted by E. faecium LKE12 and its cell-free extract inoculated in oriental melon plants revealed a favorable interaction of E. faecium LKE12 with plants. Higher plant growth rates and nutrient contents of magnesium, calcium, sodium, iron, manganese, silicon, zinc, and nitrogen were found in cell-free extract-treated plants than in control plants. The results of the current study suggest that E. faecium LKE12 promotes plant growth by producing GAs and IAA; interestingly, the exogenous application of its cell-free culture extract can be a potential strategy to accelerate plant growth.

• Journal of Microbiology and Biotechnology
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• 제24권1호
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• pp.106-112
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• 2014

Very few plant growth-promoting rhizobacteria (PGPR) are known to produce gibberellins (GAs). The current study aimed to isolate a phytohormone-producing PGP rhizobacterium from soil and assess its potential to enhance plant growth. The newly isolated bacterium was identified as Leifsonia soli sp. SE134 on the basis of partial 16S ribosomal RNA gene sequence. Application of L. soli culture filtrate significantly increased the biomass, hypocotyl, and root lengths of cucumber seeds as compared with non-inoculated sole medium and distilled water treated controls. Furthermore, the PGPR culture was applied to the GA-deficient mutant rice cultivar Waito-C. Treatment with L. soli SE134 significantly increased the growth of Waito-C rice seedlings as compared with controls. Upon chromatographic analysis of L. soli culture, we isolated, detected and quantified different GAs; namely, $GA_1$ ($0.61{\pm}0.15$), $GA_4$ ($1.58{\pm}0.26$), $GA_7$ ($0.54{\pm}0.18$), $GA_8$ ($0.98{\pm}0.15$), $GA_9$ ($0.45{\pm}0.17$), $GA_{12}$ ($0.64{\pm}0.21$), $GA_{19}$ ($0.18{\pm}0.09$), $GA_{20}$ ($0.78{\pm}0.15$), $GA_{24}$ ($0.38{\pm}0.09$), $GA_{34}$ ($0.35{\pm}0.10$), and $GA_{53}$ ($0.17{\pm}0.05$). Plant growth promotion in cucumber, tomato, and young radish plants further evidenced the potential of this strain as a PGP bacterium. The results suggest that GA secretion by L. soli SE134 might prove advantageous for its ameliorative role in crop growth. These findings can be extended for improving the productivity of different crops under diverse environmental conditions.

• 한국식품저장유통학회지
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• 제18권4호
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• pp.497-501
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• 2011

본 연구는 '신고'배의 재배과정에서 만개 후 35일에 GA 도포제를 처리하여 발생하는 과대지의 변화와 과실특성 및 품질변화의 양상을 구명하고자 수행하였다. 이상발육 과대지의 발생률은 GA 도포제 무처리군 27.5%, 25 mg 처리군 52.5%로 유의한 차이를 나타냈고, 과대지수와 꽃 수는 이상발육 과대지에서 감소하였다. GA 도포제 처리 후 배생육기간 동안 GA 도포제 25 mg 처리군의 과실비대량이 무처리군에 비해 유의적으로 증가하였다. 또한 수확기 과실의 당도, 산도, 과피색은 두 군간에 차이가 없었으나 경도는 감소하였으며 과중과 바람들이 발생률은 무처리에 비하여 GA 도포제 25 mg 처리군이 유의적으로 증가하였다. 수확 후 저장기간 동안 GA 도포제 25mg 처리 과실의 경도는 무처리보다 유의적으로 낮아졌으며 중량 감모율은 저장 후 60일에 유의적으로 증가하였다.

• 한국산림과학회지
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• 제85권3호
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• pp.532-538
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• 1996

Indoor seed orchard의 가능성을 시험하기 위해 온실의 화분에서 자라는 낙엽송 접목묘와 야외에서 자라는 실생묘에 Gibberellins(GA4/7) 살포, 주입 및 몇가지 보조 처리를 사용하여 조기개화를 유도하였다. 온실의 화분에 지라는 접목묘에 대해서는 GA4/7 반복 살포 단독처리가 가장 효과적이었으나 보조 처리로서 사용된 root pruning은 상승효과를 보이 지 못했다. GA4/7 주입은 접목표의 치사를 초래하였기 때문에 유용한 방법이 아니었다. 10년생 실생묘를 이용한 야외실험에서는 GA4/7 살포와 보조처리로서 root pruning 또는 plastic mulching의 사용이 개화의 유도에 가장 효과적이었다.

• Journal of Microbiology and Biotechnology
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• 제23권5호
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• pp.623-629
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• 2013

Gibberellins (GAs) are a group of phytohormones that control many developmental processes in higher plants. We report the cloning and expression pattern of gibberellin biosynthesis genes from a new GA-producing fungus, Fusarium proliferatum (strain KGL0401). These genes sequences are deposited in the National Center for Biotechnology Information (NCBI) under accession numbers EF119831, EF119832, DQ313173, DQ313174, DQ313175, DQ313176, and DQ313177. The expression level of these genes was maximal at a 0.5 M : 0.17 M carbon : nitrogen ratio, and minimal at a 0.25 M : 0.47 M carbon : nitrogen ratio.