• Title/Summary/Keyword: Germination Ratio

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Enhancement of Seed Dehiscence by Seed Treatment with Talaromyces flavus GG01 and GG04 in Ginseng (Panax ginseng)

  • Kim, Min-Jeong;Shim, Chang-Ki;Kim, Yong-Ki;Hong, Sung-Jun;Park, Jong-Ho;Han, Eun-Jung;Kim, Seok-Cheol
    • The Plant Pathology Journal
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    • v.33 no.1
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    • pp.1-8
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    • 2017
  • Seed dehiscence of ginseng (Panax ginseng C. A. Mayer) is affected by moisture, temperature, storage conditions and microbes. Several microbes were isolated from completely dehisced seed coat of ginseng cultivars, Chunpoong and Younpoong at Gumsan, Korea. We investigated the potential of five Talaromyces flavus isolates from the dehiscence of ginseng seed in four traditional stratification facilities. The isolates showed antagonistic activities against fungal plant pathogens, such as Cylindrocarpon destructans, Fusarium oxysporum, Rhizoctonia solani, Sclerotinia nivalis, Botrytis cinerea, and Phytophthora capsici. The dehiscence ratios of ginseng seed increased more than 33% by treatment of T. flavus GG01, GG02, GG04, GG12, and GG23 in comparison to control (28%). Among the treatments, the reformulating treatment of T. flavus isolates GG01 and GG04 showed the highest of stratification ratio of ginseng seed. After 16 weeks, the reformulating treatment of T. flavus isolates GG01 and GG04 significantly enhanced dehiscence of ginseng seed by about 81% compared to the untreated control. The candidate's treatment of T. flavus GG01 and GG04 showed the highest decreasing rate of 93% in seed coat hardness for 112 days in dehiscence period. The results suggested that the pre-inoculation of T. flavus GG01 and GG04 found to be very effective applications in improving dehiscence and germination of ginseng seed.

Seed Production Method for Waxy Corn Hybrid, Chalok 2 (찰옥수수 신품종 '찰옥2호'의 안전채종 재배법)

  • 류시환;민황기;차선우;박기진;박종열;허남기
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.6
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    • pp.424-428
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    • 2003
  • This study was conducted to find out the optimum cultural method for hybrid seed production of Chalok 2. Higher seed yield was obtained when KW3 was used as a seed parent (female). KW7 was shown Higher barren stalk occurrence which caused significant seed yield reduction when it was used as a seed parent (female). The ratio of female (KW3) to male (KW7) rows with 4 to 1 had higher seed yield than that of 2:1 or 3:1. In case of using KW3 toy a seed parent, KW3 was susceptible to stem and ear rotting disease. The 35th day after silking was optimal period to harvest due to higher 100-grain weight, lower rotten grain, and higher germination rate.

Biological Characteristics of Anodic Electrolyzed Water (산성전리수의 생물학적 특성)

  • 김윤경;민병술;민중기;이종권;이윤배;류근걸;이미영
    • Korean Journal of Environmental Biology
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    • v.22 no.2
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    • pp.265-272
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    • 2004
  • Biological characteristics of anodic electrolyzed water were investigated in this study. Linear DNAs which were incubated at $4^\circ{C}$ and $25^\circ{C}$ for 10 mins in the anodic electrolyzed water were degraded about 40% and 50%, respectively. But the DNA was amplified pretty well without any degradation through polymerase chain reaction in the presence of anodic electrolyzed water. Protein degradation hardly occurred in the distilled water during entire incubation time of 7 days, while protein began to be degraded from 4 days in the anodic electrolyzed water. Rice seeds could germinate in the distilled water and anodic electrolyzed water with the same germination ratio, however, the anodic electrolyzed water inhibited the growth of roots and total length of rice seedlings in the soil. Anodic electrolyzed water did not affect the growth curve and cell number of marine alga significantly. The anodic electrolyzed water inhibited the browning of potato by inactivating 50% of polyphenol oxidase activity.

Effect of Gelatinase and Chitinase Producing Microorganism on the Growth of Soybean and Control of Stink Bug in Field

  • Lee, Yong-Seong;Jeon, Hyeon-Deok;Kim, Yun-Tae;Monkhung, Sararat;Kim, Kil-Yong
    • Korean Journal of Soil Science and Fertilizer
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    • v.50 no.2
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    • pp.71-80
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    • 2017
  • This study was conducted to investigate the application effect of Lysobacter antibioticus HS124 (gelatinase and chitinase producing microorganism; GCM) for the improvement of soybean yield and control of stink bug. Our results showed that the yield of GCM-treated soybean increased by 17.0, 20.3, 19.0, and 25.6% in the experimental field sites of Muan-gun, Sunchang-gun, Gwangju 1, and Gwangju 2, respectively, compared to the yield of the soybean obtained by conventional practice treatment (CPT); however in the Gimjae-si, the yield decreased by 10.6%. Results in both pods and seeds $plant^{-1}$ were significantly increased in Gwangju 2 by the GCM cultured broth treatment (GCMT). Ratio of 3 seeds $pod^{-1}$ in Sunchang-gun was statistically significant between GCMT and CPT, however, the result in the other field showed no significance. Germination rate was only statistically improved by GCMT in Gwangju 2 field site. GCMT reduced the appearance of stink bug in all experimental field sites except in Gimjae-si. The soybean seed damage by stink bug was no significance in all of treatments. Therefore, GCMT could improve the productivity of soybean and also control the infestation of stink bug.

Development of efficient protocol for screening of rice genotypes using physiological traits for salt tolerance

  • Kim, Sung-Mi;Reddy, Inja Naga Bheema Lingeswar;Yoon, In Sun;Kim, Beom-Gi;Kwon, Taek-Ryoun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.189-189
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    • 2017
  • Salinity is one of the major abiotic stresses that severely affect crop production throughout the world; especially rice plant which is generally categorized as a typical glycophyte as it cannot grow in the presence of salinity. Phenotypic resistance of salinity is expressed as the ability to survive and grow in a salinity condition. Salinity resistance has, at least implicitly, been treated as a single trait. Physiological studies of rice suggest that a range of characteristics (such as low shoot sodium concentration, compartmentation of salt in older rather than younger leaves, high potassium concentration, high $K^+/Na^+$ ratio, high biomass and plant vigour) would increase the ability of the plant to cope with salinity. Criteria for evaluating and screening salinity tolerance in crop plants vary depending on the level and duration of salt stress and the plant developmental stage. Plant growth responses to salinity vary with plant life cycle; critical stages sensitive to salinity are germination, seedling establishment and flowering. We have established a standard protocol to evaluate large rice germplasms for overall performance based on specific physiological traits for salt tolerance at seedling stage. This protocol will help in identifying germplasms which can perform better in the presence of different salinity treatments based on single trait and also combination of different physiological traits. The salt tolerant germplasm can be taken forward into developing better varieties by conventional breeding and exploring genes for salt tolerance.

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The Fertility and Some Agronomic Characteristics of Morus tiliaefolia Makino Growing Wild in the Korean Peninsula (韓半島에 자생하는 돌뽕나무(Morus tiliaefolia Makino)의 稔性 및 재배학적 특성)

  • 박광준
    • Journal of Sericultural and Entomological Science
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    • v.44 no.1
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    • pp.9-12
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    • 2002
  • The agronomic characteristics and fertility of Morus tiliaefolia growing wild in Korean peninsula are as follows. The possibility of seed formation is 46∼80% and that of germination is 19∼28%. The height period of making flower is April 4-May 3, it means that is later than Morus bombycis, faster than Kaeryangppong. Green tip sprouting stage in spring is April 21∼24. This is later than Morus bombycis and faster than Kaeryangppong. The leaf has very big width and the ratio of leaf length to leaf width is 1.05. The thickness of leaf is thicker than Morous bombycis. There are a lot of matter of soluble nitrogen free, crude ash, and are little crude protein, crude fat and crude fiber in chemical contents of maturation leaf. The attitude of low cutting is spread out type and the branch is long type. The internord length is long. It is weak against cold and die-back.

Screening Method for Non-viable Seeds in Brassicaceae Vegetable Crops by Sinapine Leakage (Sinapine누출을 이용한 십자화과 채소의 퇴화종자 선별법)

  • 민태기
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.5
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    • pp.473-479
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    • 1994
  • A seed coating system was developed to screen non-viable seeds in the Brassicaceae. The crops studied were radish, chinese cabbage, broccoli, cauliflower and brussel sprout. Sinapine leaked more from artificially deteriorated seeds than non-deteriorated seeds. Seed coating with cellulose was to trap the sinapine leakage in the non-viable Brassicaceae vegetable seeds. The seeds were first hydrated, then coated with cellulose powder to capture the leakage. Coated seeds were dried, then sorted two fractions-fluorescent seeds and non-fluorescent seeds under the UV light. The ratio of the fluorescent seeds were higher in bad seedlot than good one. The germination rate were increased 3∼35% by eliminating the fluorescent seeds in tested Brassica vegetable seeds. Sowing non-fluorescent seeds resulted in a greater percent normal seedling than non-coated seeds in all seedlots. The fluorescent seeds contained a high percentage of the dead and abnormal seedlings.

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Inheritance of Agronomic Traits and Their Interrelationship in Mungbean(Vigna radiata(L.) Wilczek)

  • Sriphadet, Sukhumaporn;Lambrides, Christopher J.;Srinives, Peerasak
    • Journal of Crop Science and Biotechnology
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    • v.10 no.4
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    • pp.249-256
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    • 2007
  • A study was conducted to observe the variation and inheritance of agronomic traits and their interrelationship in mungbean. The objective of the study was to compare agronomic traits and hardseed percentage of 268 recombinant inbred lines(RILs) developed from the cross between wild Vigna subspecies sublobata "ACC 41" with the mungbean cultivar "Berken". The RIL population and their parents were evaluated under controlled conditions in a glass house at the University of Queensland, Brisbane, Australia. The results showed significant differences among the RILs and among the parents in all traits under study. Berken had a longer flowering date and a higher seed weight per plant, but less total leaf number and pod number per plant than ACC 41. A germination test between papers revealed that ACC 41 was 100% hard-seeded and did not germinate at all, while Berken germinated up to 100%. Their RILs distributed well between 0 to 100% hardseed. Upon scarification, all hardseed germinated within seven days. Narrowsense heritability estimates of total leave number, hardseedness, pod length, and pod width were highly heritable at 89.9, 98.9, 93.7, and 93.2%, respectively. The heritability of seed weight per plant and number of seeds per plant were lower at 63.1 and 58.4%, respectively. Seed weight per plant showed positive transgressive segregation when compared with ACC 41 and a positive correlation with 100 seed weight. While the number of seeds per pod showed a negative transgressive segregation when compared with Berken and a negative correlation with pod length and pod width. The RILs gave a 1:1 segregation ratio in leaflet shape, growth habit, and growth pattern, indicating that these traits were controlled by a single dominant gene.

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Composting Characteristics of Food Waste - Poultry Manure Mixture Inoculated with Effective Microorganisms (유용미생물처리 음식물쓰레기와 계분 혼합물 퇴비화 특성)

  • Hong, Ji-Hyung;Park, Keum-Joo
    • Journal of Animal Environmental Science
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    • v.15 no.1
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    • pp.59-68
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    • 2009
  • This study investigated the evaluation of maturity, stability, nutrient and heavy metal from rotating drum composter of food waste amended with poultry manure composting inoculated with effective microorganisms(EM). Composting were performed for the first, drying reactor($15m^3$) 3 hours and the second, composting reactor($30m^3$) 24 hours, and parameters monitored this period included moisture content, NaCl, pH, electrical conductivity(EC), C/N ratio, organic matter(OM), nutrient content and heavy metal. Changes in compost temperature during composting were maintained constantly in the range of $60{\sim}80^{\circ}C$ using firewood boiler(450 MJ/h). We examined physicochemical parameters and heavy metals in order to assess their effectiveness as stability and maturity, nutrient and harmful indicators such as seed germination rate<60%, potassium 1>%, dm and NaCl>1%, dm at the end of the final compost. The finished compost obtained after decomposition phase at the end of the 2nd composter could not be utilized for land improvement or reclamation.

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Effect of Timing of Ethephon Treatment on the Formation of Female Flowers and Seeds from Male Plant of Hemp (Cannabis sativa L.)

  • Moon, Youn-Ho;Lee, Yoon Jeong;Koo, Sung Cheol;Hur, Mok;Huh, Yun Chan;Chang, Jae-Ki;Park, Woo Tae
    • Korean Journal of Plant Resources
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    • v.33 no.6
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    • pp.682-688
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    • 2020
  • Hemp (Cannabis sativa L.) is a dioecious plant, although monoecious plants are bred in some cultivars for fiber or seed production. Recently, hemp has received attention for medicinal use with some cannabinoids, including cannabidiol. Self-fertilization for breeding inbred lines is difficult because of dioeciousness and anemophily in hemp. This experiment was conducted to develop a self-fertilization method by forming female flowers and seeds from male plants of dioecious hemp. To induce the formation of female flowers on male plants, 500 mg L-1 of ethephon was sprayed on plants at soon, seven and fourteen days after primordia formation. The plant ratio of female flowers formation and the number of harvested seeds were increased by ethephon treatment. Female flowers of male plants have 5 stigmas in contrast to the dual stigma of female 1plants. Male plant seeds were lighter and smaller than those from female plants. Although the germination rate was lower than that of normal seeds from female plants, the seeds from male plants germinated to grow seedlings. Thus, we suggest that hemp plants should be treated with ethephon at soon after primordia formation to induce the formation of more female flowers on the male plants.