• 대한미생물학회지
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• 제34권6호
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• pp.519-532
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• 1999

Helicobacter pylori is a causative agent of type B gastritis and plays a central role in the pathogenesis of gastroduodenal ulcer and gastric cancer. To elucidate the host-parasite relationship of the H. pylori infection on the basis of molecular biology, we tried to evaluate the genomic diversity of H. pylori. An ordered overlapping bacterial artificial chromosome (BAC) library of a Korean isolate, H. pylori 51 was constructed to set up a genomic map. A circular physical map was constructed by aligning ApaI, NotI and SfiI-digested chromosomal DNA. When the physical map of H. pylori 51 was compared to that of unrelated strain, H. pylori 26695, completely different restriction patterns were shown. Fifteen known genes were mapped on the chromosome of H. pylori 51 and the genetic map was compared with those of strain 26695 and J99, of which the entire genomic sequences were reported. There were some variability in the gene location as well as gene order among three strains. For further analysis on the genomic diversity of H. pylori, when comparing the genomic structure of 150 H. pylori Korean isolates with one another, genomic macrodiversity of H. pylori was characterized by several features: whether or not susceptible to restriction digestion of the chromsome, variation in chromosomal restriction fingerprint and/or high frequency of gene rearrangement. We also examined the extent of allelic variation in nucleotide or deduced amino acid sequences at the individual gene level. fucT, cagA and vacA were confirmed to carry regions of high variation in nucleotide sequence among strains. The plasticity zone and strain-specific genes of H. pylori 51 were analyzed and compared with the former two genomic sequences. It should be noted that the H. pylori 51-specific sequences were dispersed on the chromosome, not congregated in the plasticity zone unlike J99- or 26695-specific genes, suggesting the high frequency of gene rearrangement in H. pylori genome. The genome of H. pylori 51 shows differences in the overall genomic organization, gene order, and even in the nucleotide sequences among the H. pylori strains, which are far greater than the differences reported on the genomic diversity of H. pylori.

• 한국식품과학회지
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• 제39권5호
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• pp.580-583
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• 2007

본 연구에서는 국내 콩 자원의 lutein 함량 변이를 분석함으로써 우수 품종을 선발하고 신품종 육성연구와 기능성 식품 개발을 위한 우수 품종을 선발하고자 하였다. 본 연구에서는 장려품종 23종과 육성품종 140종(장콩품종 56종, 나물콩품종 44종, 지역재래품종 40종)을 lutein 분석에 사용하였다. 검화방법을 이용하여 lutein을 추출하였고 역상 HPLC를 이용하여 분석하였다. 연구 결과 장려품 23종의 평균 lutein 함량은 $240.27{\pm}90.74{\mu}g/100g$으로 IT No. 24634가 $402.48{\mu}g/100g$으로 가장 높은 함량을 나타내었다. 장콩은 $777.73{\mu}g/100g$으로 IT No. 153398이, 나물콩은 $588.88{\mu}g/100g$으로 IT No. 25490이, 지역재래종은 $861.07{\mu}g/100g$으로 IT No. 22564가 높은 lutein 함량을 나타내었다. 장려품종과 육성품종간의 그 평균치는 통계학적으로 유의적인 차이를 나타내지 않았으나 장려품종 보다는 육성품종에서 상대적으로 높은 lutein 함량을 나타내는 품종이 많은 것으로 나타났다. 본 연구결과는 국내 콩 유전자원의 품종개량 및 고 부가가치 기능성 식품의 개발에 대한 기초 자료가 될 것으로 생각된다.

• Reproductive and Developmental Biology
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• 제36권3호
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• pp.183-188
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• 2012

The bovine fatty acid binding protein 4 and 5 (FABP4 and 5) is a major positional and physiological candidate gene for the bovine marbling and carcass weight. The aim of this study was to evaluate the association between economic traits of Korean cattle (Hanwoo) and genetic variation in fatty acid binding protein 4 and 5 (FABP4 and 5) genes within carcass/meat quality traits and the before/after of fatting in breed Hanwoo. Here, we characterized the nucleotide polymorphism of FABP4 and 5 in 86 cattle. We were detected the variability of three types (GG, AG, and AA) by PCR, and economic traits were analyzed by the mixed regression model implemented in the ASReml program. As the result of statistical and supersonic analysis, FABP4 gene was highly showed significant effect (p<0.006) on marbling score (MS), in contrast FABP5 gene was lowed (p<0.084) on MS before fatting. But, FABP4 gene was highly showed significant effect (p<0.0054) on MS, in contrast FABP5 gene lowest (p<0.0899) on MS in the after of fatting. Compare to supersonic result before fatting in FABP4 gene, it was detected type GG: (p<7.18), AG: (p<8.50), and AA: (p<10.50) (n=50), showed type GG: (p<4.88), AG: (p<2.33), and AA: (p<0.00) after weed out (n=20). Futhermore, it was detected type GG: (p<9.30), AG: (p<7.95), and AA: (p<7.40) (n=50) before fatting in the FABP5 gene. It was shown type GG: (p<2.67), AG: (p<3.50), and AA: (p<5.00) after weed out (n=50). Our results indicate that FABP4 and 5 gene transcription is regulated by the environment of feeding and management, and suggest that feeding and management could be potential key in determining FABP4 and 5 genes transcription for carcass/meat quality traits in breed Hanwoo.

• 한국수산과학회지
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• 제30권1호
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• pp.88-94
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• 1997

황해 서식 대하(P. chinenesis) 각 개체군의 유전 구조를 파악하기 위하여 미토콘드리아 DNA(mtDNA)를 추출한 후 BamHI등 15종의 제한효소를 이용한 제한효소 절편 다형 현상 (Restriction Fragment Length Polymer-phisms; RFLPs)을 분석하였으며 보리새우 (P. japonicus)와의 염기 치환 정도를 비교하기 위하여 일본 나가사키 1 집단의 절편 양상도 함께 분석하였다. 한국의 대천, 진도, 나로도 집단과 중국의 발해, 청도 집단 등 5개 집단을 분석한 결과, 대하에서는 3종의 haplotype이 발견되었으나 5개 집단 모두가 하나의 haplotype에 의해 지배되고 있는 것으로 나타났다. 나타난 변이는 2종류로 Cla I과 Pvu II 인식부위가 부가된 변이가 청도와 나로도 집단에서 출현하였을 뿐 전 집단에 대한 나머지 13종의 제한 효소 절편 양상은 모두 동일한 것으로 나타났다. 이와 같은 결과에서 보면 황해산 대하의 각 집단 사이에는 활발한 유전자 교류가 있는 것으로 판단되며, 3개 계군에 대한 지금까지의 구분은 재검토되어야 할 것으로 판단된다. 대하와 보리새우의 mtDNA 공통절편을 이용한 p 값에 의한 염기치환은 $13.7\%$ 정도인 것으로 나타났으며, 제한 효소별 절편의 크기를 비교한 결과 대하의 mtDNA의 크기는 평균 16.44kb였고 보리새우는 약 16.31kb였다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권9호
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• pp.1303-1308
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• 2003

Samples of eleven brown midrib (ICSU 96031, ICSU 93046, ICSU 96082, ICSU 96078, ICSU 96075, ICSU 95101, ICSU 96034, ICSU 96063, ICSU 45116, ICSA 93-3 and ICSA 3845 X 3816) and nine white midrib genotypes (ICSU 96050, ICSU 96030, ISU 95082, SSG 59-3, FSHI 93-1, FSHI 2219A X 3211, HC 171, ICSA 93-2 and ICSA 93-1) based on their phenotypic appearance were collected at 50 per cent flowering from the sorghum germplasm grown at Research farm of IGFRI, Jhansi. These genotypes were evaluated with respect to crude protein, fiber composition, in-sacco dry matter, OM, cell wall components disappearance/digestibility besides the fodder yield, total phenolic and availability index values. Brown midrib genotypes were lower (p<0.05) in NDF, ADF, cellulose and acid detergent lignin concentration than white midrib genotypes. Mean NDF, ADF, cellulose and lignin contents were 69.4, 42.1, 35.4 and 5.7% in brown mid rib vis-a vis 75.8, 47.5, 39.6 and 7.3% in white mid rib genotypes. Nonsignificant (p>0.05) differences were observed in dry matter, crude protein and organic matter contents between brown midrib and white midrib genotypes. Phenolic contents were significantly (p<0.05) lower in browm mid rib (0.2) than white mid rib (0.3%) sorghum. Brown midrib genotypes exhibited significantly (p<0.05) higher in-sacco DM, OM and CP disappearance than normal (white midrib) genotypes. The mean degradability of DM, OM and CP was 64.1, 62.6 and 79.6% in brown mid rib and 53.1, 54.0 and 76.6% in white mid rib genotypes, respectively. There were no significant (p>0.05) differences between genotypes in extent of fiber fraction degradability though in-sacco NDF and ADF degradability was more by 5 and 4 units, respectively in brown midrib genotypes vis-a-vis white midrib genotypes. Average fodder yield (green and dry g/plant) and availability index (%) values were significantly (p<0.05) higher for brown midrib (474.2, 129.8 and 80.4) genotypes than white midrib (375.0, 104.8 and 69.2) genotypes. Lignin contents had significant negative correlation with DM, OM, NDF and ADF degradability. The results of the study revealed that brown midrib genotypes are superior not only with regard to chemical entities and disappearance of DM and fiber fractions but also better in respect of fodder yield and availability index values. Thus, brown midrib sorghum strains may be useful in increasing digestibility, intake, feed efficiency and animal performance.

• Asian-Australasian Journal of Animal Sciences
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• 제17권1호
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• pp.1-6
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• 2004

The 490 and 380 performance records of Karan Fries and Karan Swiss cows developed through crossbreeding followed by inter-se mating were evaluated for production, reproduction performance and disposal rate. Duration of study (1982-92) was grouped into five periods (1982-83; 1984-85; 1986-87; 1988-89; 1990-92) and each year was divided into four seasons (Winter: Dec.-Jan.; Spring: Feb.-March; Summer: April-June; Rainy: July-Sept.; Autumn: Oct.-Nov). Data were also classified according to sire and level of inbreeding. The least squares means of FLY (first lactation yield -305 days), FLL (first lactation length), FSP (first service period), FDP (first dry period), FCI (first calving interval ), MY/FLL (milk yield per day of first lactation length), MY/FCI (milk yield per day of first calving interval), EBV (expected breeding value) and EBE (expected breeding efficiency) were $3,173{\pm}82$ kg, $34611{\pm}$days, $143{\pm}11$ days, $75{\pm}6$ days, $423{\pm}11$ days, $10.6{\pm}0.2$ kg, $8.9{\pm}0.2$ kg, $3,380{\pm}26$ kg, and $88.2{\pm}1.3$% respectively in Karan Fries. Corresponding estimates in Karan Swiss cows were $2,616{\pm}82$ kg, $328{\pm}8$ days, $148{\pm}12$ days, $103{\pm}9$ days, $435{\pm}13$ days, $8.9{\pm}0.2$ kg, $7.2{\pm}0.3$ kg, $2,924{\pm38}$ kg and $86.0{\pm}3.8$% respectively. The effect of sire was significant on FLY, MY/FLL, MY/FCI and EBV in both the herd (p<0.01). The 10 and 15% sires showed superiority (FLY) over herd average of 11 and 7% in Karan Fries and 32 and 21% in Karan Swiss cattle respectively. Inbreeding has adversely and significantly (p<0.05) affected the FLY, MY/FLL, MY/FCI and survivability of Karan Fries females; FDP and disposal through culling of Karan Swiss heifers. The Karan Fries heifers with inbreeding above 12.5% performed 16% lower FLY to herd average. The effect of season of calving was significant on FLL, FSP and FCI (p<0.05) in Karan Fries. Summer calvers ($361{\pm}12$) were have higher lactation length and autumn calvers ($329{\pm}14$) had minimum. Period of calving significantly influenced the FSP, FCI, MY/FLL and MY/FCI in Karan Fries and FLY, MY/FLL and MY/FCI in Karan Swiss. Production efficiency traits in Karan Fries herd witnessed higher yield in the last two periods whereas, Karan Swiss heifers showed fluctuating performance. The findings suggested judicious use of available genetic variability, keeping of inbreeding under safer level and managemental interventions for the consistent improvement of both herds.

• Journal of Nutrition and Health
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• 제35권8호
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• pp.870-879
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• 2002

The purpose of this study was to investigate the obesity and state of dietary intake of 216 young Korean females, and the influence of $\beta$-II, III Adrenergic receptor (AR) gene polymorphism upon obesity and dietary intake. The average weight, height and BMI of the subjects were 160 cm, 54 kg, and 20.9 kg/$m^2$, respectively. The average triceps skinfold thickness, waist circumference, hip circumference and WHR were 21.7mm, 73.1cm, 93.3cm and 0.78, respectively. The results of body composition measurement using bioimpedance method, average body fluid, body protein, mineral mass and body fat were 29.271, 7.22 kg, 6.79 kg and 19.16 kg, respectively. A dietary survey was conducted using 24-hour recall method. Average calorie intake was 1621 ㎉, which is 81% of Korean RDA. We detected 182 (84.3%) Gln27 (QQ) homozygotes and 34 (15.7%) Gln27Glu (QE) heterozygotes for $\beta$-II AR polymorphism. For $\beta$-III AR polymorphism, we detected 163 (75.5%) Trp64 (WW) and 53 (24.5%) Trp 64Arg (WR). The results of comparing of obesity by $\beta$-II AR gene polymorphism, obesity index and BMI of QE type were slightly higher than those of the QQ type. For $\beta$-III AR gene polymorphism, the mean BMI, obesity index, fat mass and percent body fat (%) of the WR type were significantly higher than those of the WW type (p < 0.05). These findings suggest that genetic variability in the human $\beta$-III AR is associated with obesity among young Korean females. We also evaluated the effect of the simultaneous presence of the $\beta$-II AR and $\beta$-III AR polymorphism on obesity. We found that the BMI and obesity index of the mutant type in both $\beta$-II AR and $\beta$-III AR were significantly higher than those of the type that has only one gene mutation or has no mutation (p < 0.05), indicating a synergistic effect of $\beta$-II AR and $\beta$-III AR polymorphism on obesity. No association was found between $\beta$-II Ad or $\beta$-III AR polymorphism and dietary intake.

• Asian-Australasian Journal of Animal Sciences
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• 제31권8호
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• pp.1119-1126
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• 2018

Objective: Present investigation was aimed to study the Single Nucleotide Variants of the luteinizing hormone beta ($LH{\beta}$) gene and to analyze their association with the semen quality (fresh and post-thawed frozen semen) and luteinizing hormone (LH) concentrations in Murrah buffalo bulls. Methods: Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and Sanger sequencing method is used to study genetic variability in $LH{\beta}$ gene. LH assay was carried out using enzyme-linked immunosorbent assay method. A fixed general linear model was used to analyze association of single nucleotide polymorphism (SNP) of $LH{\beta}$ gene with semen quality in 109 and LH concentrations in 80 Murrah bulls. Results: $LH{\beta}$ gene was found to be polymorphic. Total six SNPs were identified in $LH{\beta}$ gene g C356090A, g C356113T, g A356701G, g G355869A, g G356330C, and g G356606T. Single Stranded Conformational Polymorphism variants of pattern 2 of exon 1+pattern 2 of exon 2+pattern 1 of exon 3 had highly significant (p<0.01) effect on sperm concentration (million/mL), percent mass motility, acrosome integrity and membrane integrity in fresh and frozen semen whereas significant (p<0.05) effect was observed on percent live spermatozoa. SSCP variants of pattern 2 of exon 1+pattern 2 of exon 2+pattern 1 of exon 3 had highly significant (p<0.01) effect on luteinizing hormone concentrations too. Conclusion: The observed association between SSCP variants of $LH{\beta}$ gene with semen quality parameters and LH concentrations indicated the possibilities of using $LH{\beta}$ as a candidate gene for identification of markers for semen quality traits and LH concentrations in Murrah buffaloes.

• 식물분류학회지
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• 제45권4호
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• pp.353-361
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• 2015

난초 열매의 결실의 시 공간적 변화가 장기적으로 개체군의 생존에 영향을 끼칠 수 있으며 수 세대에 걸쳐 유전적 다양성에 영향을 미칠 것이다. 본 연구의 목적은 화밀을 분비하는 육상 난초인 닭의난초를 대상으로 자연 조건에서 시 공간의 결실 수준을 조사하고 교배계를 파악하는 데 있다. 본 연구자들은 전라남도 해남군 징의리 해안 1.5 km 걸쳐 위치한 4곳 집단에서 2년간의 조사 기간 동안 수분실험을 행하였다. 2년 동안 결실률이 집단 내 작은 슈트의 모임인 패치 내에서 유사했다. 대조적으로, 패치 간에는 결실룰이 유의 한 수준에서 차이를 보였다. 또한 큰 화서를 지닌 식물이 작은 화서를 지니는 식물보다 훨씬 더 많은 열매 생성을 보였다. 닭의난초의 결실률은 2 년의 조사 기간 동안 비슷하였으나(14.1%) 연구된 보상을 주는(rewarding) 난초84종의 평균 결실률(37.1%) 보다 낮았다. 인위적인 자가 수분(90.5-95.2%), 인위적인 인화(隣花)수분(geitonogamy: 94.7-95.0%), 그리고 인위적인 타가 수분(91.3-91.4%)등에 의해서는 결실률이 상당한 수준으로 증가되었다. 꽃피기 전 수술을 제거 한 경우에 열매가 전혀 맺히지 않았으며 꽃 스스로 자동으로 수분이 일어나지 않았다. 본 연구 결과에 의하면 닭의난초는 자가화합성이고, 열매를 생성하기 위해서는 수분매개체인 곤충류가 필요하며, 2년간 조사 기간 중 징의리 집단들의 환경이 비슷하였음을 암시하고 있다. 본 연구 결과는 결실률이 시간보다 공간적으로 더욱 뚜렷하다는 점을 강조하고 있다.

• 한국축산식품학회지
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• 제40권5호
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• pp.689-698
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• 2020

The aim of study was to scrutinize the physicochemical and protein profile of milk obtained from local Pakistani breeds of milch animals such as Nilli-Ravi buffalo, Sahiwal cow, Kajli sheep, Beetal goat and Brela camel. Physicochemical analysis unveiled maximum number of total solids and protein found in sheep and minimum in camel. Buffalo milk contains the highest level of fat (7.45%) while camel milk contains minimum (1.94%). Ash was found maximum in buffalo (0.81%) and sheep (0.80%) while minimum in cow's milk (0.71%). Casein and whey proteins were separated by subjecting milk to isoelectric pH and then analyzed through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed heterogeneity among these species. Different fractions including α_S1, α_S2, κ-casein, β-casein and β-lactoglobulen (β-Lg) were identified and quantitatively compared in all milk samples. Additionally, this electrophoretic method after examining the number and strength of different protein bands (α_S1, α_S2, β-CN, α-LAC, BSA, and β-Lg, etc.), was helpful to understand the properties of milk for different processing purposes and could be successfully applied in dairy industry. Results revealed that camel milk was best suitable for producing allergen free milk protein products. Furthermore, based on the variability of milk proteins, it is suggested to clarify the phylogenetic relationships between different cattle breeds and to gather the necessary data to preserve the genetic fund and biodiversity of the local breeds. Thus, the study of milk protein from different breed and species has a wide range of scope in producing diverse protein based dairy products like cheese.