• Title/Summary/Keyword: Genetic stability

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Fuzzy Modelling and Control of Nonlinear Systems Using a Genetic Algorithm (유전알고리즘을 이용한 비선형시스템의 퍼지 모델링 및 제어)

  • Lee, Hyun-Sik;Jin, Gang-Gyoo
    • Proceedings of the KIEE Conference
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    • 1998.07b
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    • pp.581-584
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    • 1998
  • This paper presents a scheme for fuzzy modelling and control of continuous-time nonlinear systems using a genetic algorithm. A fuzzy model is characterized by fuzzy "if-then" rules whose consequence part has a linear dynamic equation as subsystem of the system. The parameters of the fuzzy model are adjusted by a genetic algorithm. Then a tracking controller which guarantees stability of the overall system is designed. The simulation result demonstrates the effectiveness of the proposed method.

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Genetic Stability of Magnaporthe oryzae during Successive Passages through Rice Plants and on Artificial Medium

  • Park, Sook-Young;Chi, Myoung-Hwan;Milgroom, Michael G.;Kim, Hyo-Jung;Han, Seong-Sook;Kang, Seog-Chan;Lee, Yong-Hwan
    • The Plant Pathology Journal
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    • v.26 no.4
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    • pp.313-320
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    • 2010
  • Genetic instability of the rice blast fungus Magnaporthe oryzae has been suggested as a major factor underlying the rapid breakdown of host resistance in the field. However, little information is available on the mechanism of genetic instability. In this study, we assessed the stability of repetitive DNA elements and several key phenotypic traits important for pathogenesis after serially transferring two isolates though rice plants and an artificial medium. Using isolate 70-15, we obtained a total of 176 single-spore isolates from 10 successive rounds of culturing on artificial medium. Another 20 isolates were obtained from germ tubes formed at the basal and apical cells of 10 three-celled conidia. Additionally, 60 isolates were obtained from isolate KJ201 after serial transfers through rice plants and an artificial medium. No apparent differences in phenotypes, including mycelial growth, conidial morphologies, conidiation, conidial germination, appressorium formation, and virulence, or in DNA fingerprints using MGR586, MAGGY, Pot2, LINE, MG-SINE and PWL2 as probes were observed among isolates from the same parent isolate. Southern hybridization and sequence analysis of two avirulence genes, AVR-Pita1 and AVR-Pikm, showed that both genes were also maintained stably during 10 successive generations on medium and plants. However, one reversible loss of restriction fragments was found in the telomere-linked helicase gene (TLH1) family, suggesting some telomere regions may be more unstable than the rest of the genome. Taken together, our results suggest that phenotype and genotype of M. oryzae isolates do not noticeably change, at least up to 10 successive generations on a cultural medium and in host plants.

An Intelligent Control of Mobile Robot Using Genetic Algorithm (유전자 알고리즘을 이용한 이동로봇의 지능제어)

  • 한성현
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.13 no.3
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    • pp.126-132
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    • 2004
  • This paper proposed trajectory tracking control based on genetic algorithm. Trajectory tracking control scheme are real coding genetic algorithm(RCGA) and back-propagation algorithm(BPA). Control scheme ability experience proposed simulation. Stable tracking control problem of mobile robots have been studied in recent years. These studies have guaranteed stability of controller, but the performance of transient state has not been guaranteed. In some situations, constant gain controller shows overshoots and oscillations. So we introduce better control scheme using real coding genetic algorithm and neural network. Using RCGA, we can find proper gains in several situations and these gains are generalized by neural network. The generalization power of neural network will give proper gain in untrained situation. Performance of proposed controller will verity numerical simulations and the results show better performance than constant gain controller.

A Design of Model Following Optimal Multivariable BOiler-Turbine H_\infty Control System using Genetic Algorithm (유전 알고리즘을 이용한 모델 추종형 최적 다변수 보일러-터빈 H_\infty제어 시스템의 세계)

  • Hwang, Hyeon-Jun;Kim, Dong-Wan;Park, Jun-Ho;Hwang, Chang-Seon
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.48 no.2
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    • pp.127-135
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    • 1999
  • Multivarialbe Boiler-Turbine H_\infty Control System Genetic Algorithm Weighting Functions $W_1$(s), $W_2$(s), and design parameter $\gamma$ that are given by Glover-Doyle algorithm, to optimally follow the output of reference model. The first method to do this is that the gains of weighting functions $W_1$(s), $W_2$(s), and design parameter are optimized simultaneously by genetic algorithm with the tournament method that can search more diversely, in the search domain which guarantees the robust stability of system. And the second method is that not only by genetic algorithm with the roulette-wheel method that can search more fast, in that search domain. The boiler-turbine H_\infty control system designed by theabove second method has not only the robust stability to a modeling error but also the the better command tracking preformance than those of the H_\infty control system designed by trial-and-error method and the above first method. Also, this boiler-turbine H_\infty control system has the better performance than that of the LQG/LTR contro lsystem. The effectiveness of this boiler-turbineH_\infty control system is verified by computer simulation.

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Production of Amylase from Intergeneric Hybrids between 'Aspergillus oryzae var oryzae and Penicillium Verruculosum F-3 by Nuclear Transfer (핵전이에 대한 Aspergillus oryzae var oryzae와 Penicillium verruculosum F-3의 잡종으로부터 Amylase 생산)

  • Yang, Young-Ki;Park, Yeol;Kim, sung-Joon;Moon, Myung-Nim;Park, Hyung-Nam;Lim, Chae-Young;Rhee, Young-Ha
    • Microbiology and Biotechnology Letters
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    • v.22 no.1
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    • pp.23-30
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    • 1994
  • Intergeneric hybrids formed between Aspergillus oryzae var oryzae and Penicillium verruculosum F-3 were obtained by nuclear transfer technique. Several auxotrophic mutants isolated fromconidio-spores of the two strains mutagenized with ultraviolet and N-methyl-N-nitrosoguanidine. Optimal conditions for formation of intergeneric hybrids were investigated. Frequencies of hybrid formation by nuclear transfer were 3$\times $10$^{-5}$ $~$1$\times $10$^{-5}$. From observation of genetic stability, conidial size, DNA content, nuclear stain, it was suggested that their karyptypes are aneuploid. The hybrid posses the 1.3$~$2.2 fold higher amylase activities than those of parental strains. It was also revealed that some hybids had different isozyme patterns compared to those of parental strains by amylase activity assays.

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Genetic Stability of the Integrated Structural Gene of Guamerin in Recombinant Pichia pastoris

  • Lim, Hyung-Kwon;Kim, Kyeong-Yeon;Lee, Kong-Ju;Park, Doo-Hong;Chung, Soo-Il;Jung, Kyung-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.10 no.4
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    • pp.470-475
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    • 2000
  • Genetic chracterstics of the structural gene of guamerin (a novel elastase inhibitor from Korean leech), integrated into the HIS4 locus of chromosomal DNA of Pichia pastoris along with the $\alpha$-factor leader sequence, were investigated. In the selected clone from candidates, two copies of the integration cassette including the structural gene copies of the integration cassette including the structural gene of guamerin were found in the integration site of the chromosomal DNA of P.pastoris. It was demonstrated that the integrated structural gene of guamerin was stable up to about 70 generations in the relay flask culture. Then, a high-cell-density culture could be fulfilled easily by DO-stat fed-batch culture, in which the cell growth and the recombinant guamerin production reached about 250 of OD600nm and 260 mg/l, respectively. Finally, it was revealed that the DNA sequence of the integrated structural gene of guamerin in P. pastoris was maintained correctly in the end of production cells of relay flask culture and high-cell-density culture.

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Evaluation of Immobilization Methods for Cyclodextrin Glucanotransferase and Characterization of its Enzymatic Properties

  • Lee, Sang-Ho;Shin, Hyun-Dong;Lee, Yong-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.1 no.1
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    • pp.54-62
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    • 1991
  • Cyclodextrin glucanotransferase(CGTase) derived from Bacillus macerans was immobilized by (1) covalent linkage on chitosan and chitin with glutaraldehyde, (2) adsorption on DEAE-cellulose and Amberite IRA 900 after succinylation, and (3) entrapment on alginate and polyacrylamide by cross linking. Adsorption on Amberite IRA 900 and covalent linking on chitosan were identified to be the most suitable immobilization methods considering the yield of activity and stability of immobilized CGTase. The enzymatic properties of immobilized CGTase were investigated and compared with those of the soluble CGTase. Thermal stability of CGTase immobilized on chitosan was increased from 50 to $55^{\circ}C$, and the optimum temperature of CGTase immobilized on Amberite IRA 900 was shifted from 55 to $50^{\circ}C$. The effect of molecular size of soluble starch (substrate) on immobilized CGTase investigated using partially liquefied substrates with different dextrose equivalent(DE). Cyclodextrin(CD) conversion yield augmented according to the increase of DE level for immobilized CGTase on Amberite IRA 900. CD conversion yield of partially cyclized starch with soluble CGTase was higher compared with liquefied one with ${\alpha}-amylase$.

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A Design of Optimal PI Controller of SVC System using Genetic Algorithms (유전 알고리즘을 이용한 SVC 계통의 최적 PI 제어기 설계)

  • Jeong, Hyeong-Hwan;Heo, Dong-Ryeol;Wang, Yong-Pil;Han, Gil-Man;Kim, Hae-Jae
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.49 no.5
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    • pp.212-219
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    • 2000
  • This paper deals with a systematic approach to GA-PI controller design for static VAR compensator(SVC) using genetic algorithms(GAs) which are search algorithms based on the mechanics of natural of natural selection and natural genetics, to improve system stability. A SVC, one of the Flexible AC Transmission System(FACTS), constructed by a fixed capacitor(FC) and a thyristor controlled reactor(TCR), is designed and implemented to improve the damping of a synchronous generator, as well as controlling the system voltage. To verify the robustness of the proposed method, considered dynamic response of generator used deviation and generator terminal voltage by applying a power fluctuation and three-phase fault at heavy load, normal load and light load. Thus, we proved usefulness of GA-PI controller design to improve the stability of single machine-infinite bus with SVC system.

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Optimum Design of a Composite T-tail Configuration for Maximum Flutter Speed Using Genetic Algorithm (유전자 알고리즘을 이용한 T-형 복합재료 날개의 플러터 속도 최적설계)

  • Alexander, Boby;Oh, Se-Won;Kim, Dong-Hyun
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2005.11a
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    • pp.173-178
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    • 2005
  • In this paper, an efficient and robust analysis system for the flutter optimization of laminated composite wings has been developed using the coupled computational method based on the genetic algorithm. General three-dimensional doublet-lattice method is efficiently used to compute generalized aerodynamic forces of T-tail configuration in the frequency domain. Structural dynamic analyses of laminated composite T-tail models are conducted using finite clement method. The classical P-k flutter analysis technique is applied to effectively solve the aeroelastic governing equations in the frequency domain. Optimum design studies using genetic algorithm have been conducted in order to obtain maximum flutter stability of a composite T-tail configuration. The results show that flutter stability can be significantly increased using composite materials with proper optimum design concepts even for the same weight and shape condition. In the view point of engineering design, it is also importantly shown that the optimization of the vertical wing part is highly effective comparing to the optimization of horizontal wing part.

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