Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Production of Amylase from Intergeneric Hybrids between 'Aspergillus oryzae var oryzae and Penicillium Verruculosum F-3 by Nuclear Transfer

핵전이에 대한 Aspergillus oryzae var oryzae와 Penicillium verruculosum F-3의 잡종으로부터 Amylase 생산

  • Yang, Young-Ki (Department of Genetic Engineering, Chosun University) ;
  • Park, Yeol (Department of Genetic Engineering, Chosun University) ;
  • Kim, sung-Joon (Department of Genetic Engineering, Chosun University) ;
  • Moon, Myung-Nim (Department of Genetic Engineering, Chosun University) ;
  • Park, Hyung-Nam (Department of Genetic Engineering, Chosun University) ;
  • Lim, Chae-Young (Department of Genetic Engineering, Chosun University) ;
  • Rhee, Young-Ha (Department of Microbiology, Chungnam National University)
  • 양영기 (조선대학교 자연과학대학 유전공학과) ;
  • 박열 (조선대학교 자연과학대학 유전공학과) ;
  • 김성준 (조선대학교 자연과학대학 유전공학과) ;
  • 문명님 (조선대학교 자연과학대학 유전공학과) ;
  • 박형남 (조선대학교 자연과학대학 유전공학과) ;
  • 임채영 (조선대학교 자연과학대학 유전공학과) ;
  • 이영하 (충남대학교 자연과학대학 미생물학과)
  • Published : 1994.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

Intergeneric hybrids formed between Aspergillus oryzae var oryzae and Penicillium verruculosum F-3 were obtained by nuclear transfer technique. Several auxotrophic mutants isolated fromconidio-spores of the two strains mutagenized with ultraviolet and N-methyl-N-nitrosoguanidine. Optimal conditions for formation of intergeneric hybrids were investigated. Frequencies of hybrid formation by nuclear transfer were 3$\times $10$^{-5}$ $~$1$\times $10$^{-5}$. From observation of genetic stability, conidial size, DNA content, nuclear stain, it was suggested that their karyptypes are aneuploid. The hybrid posses the 1.3$~$2.2 fold higher amylase activities than those of parental strains. It was also revealed that some hybids had different isozyme patterns compared to those of parental strains by amylase activity assays.

Keywords

References

  1. Nature v.262 Somatic hybridization of Penicillium roquefortii with P. chrysogenum after protoplast fusion Anne,J.;H.Eyssen;P.Desomer
  2. Anal. Biochem. Detection of cellulase activity in polyacrylamide gels using congo red-stained agar replicas Beguin,P.
  3. Can. J. Microbiol. v.27 Isolation of protoplasts from Aspergillus nidulans conidiospores Bos,C.J.;S.M.Slakhort
  4. Biotechnology(2nd ed.) Crueger,W.;A.Crueger
  5. Ann. N.Y. Acad. Sci. v.121 Disc electrophoresis-Ⅱ Davis,B.J.
  6. Curr. Microbiol. v.7 Transfer of nuclei into protoplasts of Saccharomyces cerevisiae Ferenczy,L.;M.Pesti
  7. Arch. Microbiol. v.115 Recombination after protoplast fusion in the yeast Candida tropicalis Fournier,P.;A.Provost;C.Bourguibnon;H.Heslet
  8. Nature v.206 An improved diphenylamine method for estimation of deoxyribonucleic acid Giles,K.;W.Myers
  9. Enzyme Microb. Technol. v.3 Efficient protoplast isolation from fungi using commercial enzymes Hamlyn,P.F.;R.E.Bradshaw
  10. Mut. Res. v.48 Genetic damage induced by ethyl alcohol in Aspergillus nidulans Harsangyi,Z.;I.A.Graneck;D.W.R.Mackenzie
  11. Kor. J. Microbiol. v.22 The conidial protoplast fusion of cellulolytic fungus Trichoderma koningii Hong,S.W.;Y.C.Hah;H.M.Park
  12. M. S. thesis. Chunnam National University The protoplast fusion from conidiospore of Penicillium verruculosum Kim,J.H.
  13. Nature v.227 Cleavage of structual proteins during the assembly of the head of bacteriophage T4 Laemmli,U.K.
  14. Kor. J. Appl. Microbiol. Bioeng. v.12 Strain development of Zymomonas mobilis for ethanol production Lee,K.J.
  15. Trans. Br. Mycol. Soc. v.89 Virus transmission through intraspecific protoplast fusion in Aspergillus Liang,P.L.;C.Kaiying
  16. Theor. Appl. Genet. v.53 Investigation on the transfer of isolated nuclei into protoplasts Lorz,H.;I.Potrycus
  17. M.S. thesis. Chungnam National University Study on interspecific hybrid formation by nuclear transfer in Aspergillus sp. Rho,H.S.
  18. Appl. Environ. Microbiol. Transfer of isolated nuclei into protoplast of Trichoderma harzianum Sivan,A.;G.E.Harman;T.E.Starz
  19. J. Biol. Chem. v.195 Notes on sugar determination Somogyi,M.
  20. Bacterial and mold amylase: the enzyme Takagi,T.;H.Toda;T.Isoemura;P.D. Boyer, N.Y.(ed.)
  21. Appl. Environ. Microbiol. v.47 Protoplast fusion of Trichoderma reesei using immature conidia Toyama,H.;K.Yamaguchi;A.Shinmyo;H.Okada
  22. Kor. J. Microbiol. v.17 Study on formation of Aspergillus nidulans hybrids by protoplast fusion and nuclear transfer Yang,Y.K.;Y.Park;Y.H.Rhee;P.J.Maeng