Production of Amylase from Intergeneric Hybrids between 'Aspergillus oryzae var oryzae and Penicillium Verruculosum F-3 by Nuclear Transfer

핵전이에 대한 Aspergillus oryzae var oryzae와 Penicillium verruculosum F-3의 잡종으로부터 Amylase 생산

  • Yang, Young-Ki (Department of Genetic Engineering, Chosun University) ;
  • Park, Yeol (Department of Genetic Engineering, Chosun University) ;
  • Kim, sung-Joon (Department of Genetic Engineering, Chosun University) ;
  • Moon, Myung-Nim (Department of Genetic Engineering, Chosun University) ;
  • Park, Hyung-Nam (Department of Genetic Engineering, Chosun University) ;
  • Lim, Chae-Young (Department of Genetic Engineering, Chosun University) ;
  • Rhee, Young-Ha (Department of Microbiology, Chungnam National University)
  • 양영기 (조선대학교 자연과학대학 유전공학과) ;
  • 박열 (조선대학교 자연과학대학 유전공학과) ;
  • 김성준 (조선대학교 자연과학대학 유전공학과) ;
  • 문명님 (조선대학교 자연과학대학 유전공학과) ;
  • 박형남 (조선대학교 자연과학대학 유전공학과) ;
  • 임채영 (조선대학교 자연과학대학 유전공학과) ;
  • 이영하 (충남대학교 자연과학대학 미생물학과)
  • Published : 1994.02.01

Abstract

Intergeneric hybrids formed between Aspergillus oryzae var oryzae and Penicillium verruculosum F-3 were obtained by nuclear transfer technique. Several auxotrophic mutants isolated fromconidio-spores of the two strains mutagenized with ultraviolet and N-methyl-N-nitrosoguanidine. Optimal conditions for formation of intergeneric hybrids were investigated. Frequencies of hybrid formation by nuclear transfer were 3$\times $10$^{-5}$ $~$1$\times $10$^{-5}$. From observation of genetic stability, conidial size, DNA content, nuclear stain, it was suggested that their karyptypes are aneuploid. The hybrid posses the 1.3$~$2.2 fold higher amylase activities than those of parental strains. It was also revealed that some hybids had different isozyme patterns compared to those of parental strains by amylase activity assays.

Keywords

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