• 한국농림기상학회지
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• 제14권2호
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• pp.71-78
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• 2012

본 연구에서는 Eupatilin이 고함유된 약쑥의 생육환경을 구명하고자 하였다. 이를 위해서 먼저 선행연구를 통해 Eupatilin이 고함유된다고 알려진 강화지역의 기상학적 특성을 살펴보고, 재배지역별, 수확시기별 Eupatilin 함유량과 기상요소와의 관계를 분석 하였다. 또한 강화지역의 해양, 대기질, 토양 그리고 약쑥 생육지 분포 자료를 이용하여 환경학적 조건도 분석 하였다. 결과적으로 일조시간이 길고 일교차가 큰 지역이 유효 성분이 많이 함유된 약쑥 생산에 유리한 환경이며, 강화도는 주변 지역에 비해서 일조시간이 길며 일교차가 크기 때문에 Eupatilin 함량이 많은 약쑥의 재배 및 서식처로써 적합한 지역으로 사료되었다. 또한 강화는 약한 해풍과 주변 해역의 낮은 염분도에 의해 유입되는 염분의 양이 다른 해안지방에 비하여 낮으며 다른 지역과 비교해볼 때 청정한 대기환경을 가지고 있는 것으로 분석되었다. 이와 더불어, 강화지역은 산지 주변으로 배수가 양호한 사양 질 내지 식양 질이 많이 분포하는데 이러한 토양 분포는 약쑥 생육지 분포와 일치하였다. 즉, 적절한 염분의 유입과 청정한 대기 그리고 배수가 양호한 토양이 Eupatilin 함유에 유리한 환경이 되었을 것으로 사료되었다.

• 한국농림기상학회지
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• 제10권3호
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• pp.75-81
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• 2008

인공산성비 처리가 백합나무 묘목의 잎에 미치는 영향을 분석하기 위하여 인공적으로 pH 5.6, 4.9, 3.9, 2.9로 구분된 산성비를 일정기간 처리하여 엽록소 함량, 엽피해율 및 가시적 피해증상을 조사한 결과, 엽록소 함량의 경우, pH 수준 간 통계적으로 유의미한 차이가 나타났으나 pH 수준별 순차적 감소는 나타나지 않았고 인공산성비 처리가 지속됨에 따른 엽록소함량의 감소를 보였다. 또한, 엽록소 함량은 세 가지 토양에서 모두 pH 2.9처리구에서 가장 큰 폭으로 감소하였다. 엽피해율은 인공산성비 처리 초기시점에 급격한 피해를 보이다가 지속적으로 피해가 나타났다. 또한, pH 수준이 낮아짐으로써 엽피해율도 차이를 나타내었으며, 가시적 피해증상으로는 기형잎, 갈변현상, 괴사반점 형태로 나타났다. 본 연구의 결과를 전반적으로 고려할 때, 인공산성비 처리는 백합나무 묘목의 잎에서 일어나는 광합성작용 및 물리적 피해를 준 것으로 나타났다. 따라서 최근 가로수 및 용재수로 보급 중에 있는 백합나무에 대하여 산성비에 의해 예상되는 내외부 생리적 반응 및 생장에 미치는 영향 등에 관한 체계적이고 부가적인 연구가 요구된다.

• 한국산업보건학회지
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• 제21권2호
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• pp.116-122
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• 2011

Human occupational exposure to n-hexane has been associated with neurobehavioral symptoms such as depression, irritablity, acute irritation symptom, concentration disturbance and fatigue. Effects of monoamine oxidase (MAO) B and serotonin transporter receptor (5-HTTR) polymorphisms on the neurobehavioral symptoms were investigated in 70 male workers from TV and computer monitor manufacturing plants exposed to n-hexane. Neurobehavioral symptoms were assessed through a self-reported questionnaire and ambient level of n-hexane was measured by NIOSH method. Blood and urine were collected from each workers to determine the MAO(B), 5-HTTR and urinary 2,5-hexanedione(2,5-HD). The mean concentration of volatile n-hexane was $18.8{\pm}28.8ppm$ and that of urinary 2,5-HD was $1.07{\pm}1.47mg/g$ creatinine. Statistically significant associations with sexual disturbance were age and smoking. The frequencies of MAO(B) AA, AG and GG were 18.6%, 45.7% and 35.7%, respectively, and the frequencies of 5-HTTR ll, ls and ss genotype were 82.9%, 15.7% and 1.4%, respectively. MAO (B) gene polymorphisms had susceptibility to the neurobehavioral symptoms such as fatigue, concentration disturbance, irritability and acute irritation symptom and 5-HTTR gene polymorphism had susceptibility to the sleep disturbance and acute irritation symptom. On multiple logistic regression analysis for the neurobehavioral symptoms, memory disturbance was significantly associated with smoking(OR=6.752, 95% CI=37.46) and drinking(OR=4.033, 95% CI=1.252-12.98), emotional lability was MAO(B) genotype(OR=0.412, 95% CI=0.170-0.996), fatigue (OR=1.011, 95% CI=1.000-1.021) and acute irritation(OR=0.990, 95% CI=0.981-1.000) were working duration and sexual disturbance were significantly associated with age(OR=1.208, 95% CI=1.042-1.399), ambient n-hexane(OR=1.077, 95% CI=1.005-1.154) and 2,5-HD(OR=0.186, 95% CI=0.041-0.841). This finding implies that the MAO (B) and 5-HTTR polymorphisms may affect susceptibility for specific neurobehavioral symptoms associated with n-hexane exposure in workers.

• Biomolecules & Therapeutics
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• 제25권2호
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• pp.112-121
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• 2017

Drug-induced liver injury (DILI) is the serious and fatal drug-associated adverse effect, but its incidence is very low and individual variation in severity is substantial. Acetaminophen (APAP)-induced liver injury accounts for >50% of reported DILI cases but little is known for the cause of individual variations in the severity. Intrinsic genetic variation is considered a key element but the identity of the genes was not well-established. Here, pre-biopsy method and microarray technique was applied to uncover the key genes for APAP-induced liver injury in mice, and a cause and effect experiment employing quantitative real-time PCR was conducted to confirm the correlation between the uncovered genes and APAP-induced hepatotoxicity. We identified the innately and differentially expressed genes of mice susceptible to APAP-induced hepatotoxicity in the pre-biopsied liver tissue before APAP treatment through microarray analysis of the global gene expression profiles (Affymetrix $GeneChip^{(R)}$ Mouse Gene 1.0 ST for 28,853 genes). Expression of 16 genes including Gdap10, Lpl, Gabra3 and Ccrn4l were significantly different (t-test: FDR <10%) more than 1.5 fold in the susceptible animals than resistant. To confirm the association with the susceptibility to APAP-induced hepatotoxicity, another set of animals were measured for the expression level of selected 4 genes (higher two and lower two genes) in the liver pre-biopsy and their sensitivity to APAP-induced hepatotoxicity was evaluated by post hoc. Notably, the expressions of Gabra3 and Lpl were significantly correlated with the severity of liver injury (p<0.05) demonstrating that these genes may be linked to the susceptibility to APAP-induced hepatotoxicity.

• 식물조직배양학회지
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• 제25권4호
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• pp.245-250
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• 1998

Tobacco mosaic virus(TMV) 외피단백질 유전자를 연초(Nicotiana tabacm cv. NC82)에 형질전환하고 형질전환 식물체 후세대에서 TMV 저항성인 연초를 선발하여, 선발된 TMV 저항성 제5세대 형질전환 식물체의 도입된 유전자발현 및 고온에서의 특성 등을 조사하였다. TMV 저항성 식물체의 염색체 DNA에 TMV 외피 단백질 유전자가 안정되게 존재하고 있음을 genomic PCR을 수행하여 확인하였다. 또한 형질전환 식물체내에서 TMV 외피 단백질 발현은 Immunoblot hybridization 방법으로 확인하였다. TMV 저항성 형질전환 연초식물체에서 발현된 단백질의 양은 매우 적었으며 특히 본엽에는 병징이 나타나지 않았으나 수확기 마지막 액아에 TMV의 반점이 나타난 병징발현 지연형의 형질전환 식물체의 경우에도 발현된 단백질의 양은 정상 NC 82에 TMV가 감염되었을 때와 비교하여 현저히 적었다. TMV 저항성 형질전환 식물체 내에서 발현되는 TMV 외피단백질의 양은 총 단백질에 대비하여 0.01% 이하이였다. TMV 병징 발현 지연형인 형질전환 식물체에 TMV를 인공접종한 후 고온처리상태에서 외피 단백질 유전자의 전사 및 발현을 RT-PCR과 Immune blot hybridization 통하여 확인하였으며, 이때 TMV의 증식도 억제되었으므로 개량멀칭시 나타나는 고온조건하에서도 저항성이 안정적으로 발현될 수 있음을 알 수 있었다.

• 생명과학회지
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• 제21권2호
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• pp.194-201
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• 2011

Caudal homeodomain 단백질은 초파리에서 사람에 이르기까지 보존되어 있으며, 장조직의 발생과 유지에 필수적인 역할을 한다. CDX1과 CDX2의 발현 감소 혹은 소실이 대장암과 연관되어 있음이 잘 알려져 있다. 대장암 발생은 산화성 스트레스와 밀접한 관련이 있으며, 대장암 조직에서 항산화효소인 catalase의 발현이 감소되어 있다. 하지만 그 분자적 기전은 잘 밝혀져 있지 않다. 본 연구에서는 초파리와 사람의 caudal homeodomain 단백질들이 catalase의 발현을 조절하는 것을 보여준다. 초파리 caudal heterozygous 돌연변이체의 대장조직에서 catalase의 발현이 감소되어 있고 ROS 생성이 증가되어 있음을 관찰하였다. 그리고 caudal 유전자의 과발현에 의해 catalase promoter의 활성과 mRNA 수준이 각각 증가함을 확인하였다. 또한 사람의 대장암 세포주인 HCT116 세포에서 CDX1과 CDX2가 catalase promoter의 활성과 단백질 수준에서 catalase를 상향 조절함을 관찰하였다. 이러한 결과들은 CDX1과 CDX2가 catalase 발현 조절을 통하여 장의 항상성 유지와 암발생 과정에 관여함을 시사한다.

• Journal of Genetic Medicine
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• 제14권1호
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• pp.8-17
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• 2017

Purpose: Pulse wave velocity (PWV) is an indicator of arterial stiffness, and is considered a marker of vascular damage. However, a genome-wide association study analyzing single nucleotide polymorphisms (SNPs) associated with brachial-ankle PWV (baPWV) has not been conducted in healthy populations. We performed this study to identify SNPs associated with baPWV in healthy populations in Korea. Materials and Methods: Genomic SNPs data for 2,407 individuals from three sites were analyzed as part of the Korean Genomic Epidemiologic Study. Without replication samples, we performed multivariable analysis as a post hoc analysis to verify the findings in site adjusted analysis. Healthy subjects aged between 40 and 70 years without self-reported history or diagnosis of hypertension, diabetes, hyperlipidemia, heart disease, cerebrovascular disease and cancer were included. We excluded subjects with a creatinine level >1.4 mg/dL (men) and 1.2 mg/dL (women). Results: In the site-adjusted association analysis, significant associations (P<$5{\times}10^{-8}$) with baPWV were detected for only 5 SNPs with low minor allele frequency. In multivariable analysis adjusted by age, sex, height, body mass index, mean arterial pressure, site, smoking, alcohol, and exercise, 11 SNPs were found to be associated (P<$5{\times}10^{-8}$) with baPWV. The 5 SNPs (P<$5{\times}10^{-8}$) linked to three genes (OPCML, PRR35 and RAB40C) were common between site-adjusted analysis and multivariable analysis. However, meta-analysis of the result from three sites for the 11 SNPs showed no significant associations. Conclusion: Using the recent standard for genome-wide association study, we did not find any evidence of significant association signals with baPWV.

• 한국응급구조학회지
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• 제12권1호
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• pp.53-67
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• 2008

Purpose : This study is descriptive correlation research to provide basic materials for developing program which can give practical assistance in preventing and managing obesity through more comprehensive approaches of influential factors on obesity found in existing literatures. Methods : Data were collected from the 4th, 5th and 6th graders at 2 elementary schools in Gwangju Metropolitan City and 4 elementary schools in Jeollanamdo province using questionnaire and students' physical examination results from June 15 to July 27, 2006 and data obtained from 1,866 students were used for analysis. Data collected were analyzed with technical statistics and correlation analysis using SPSS/PC(version 12.0). Results : 1. Obesity level of elementary school students was examined as follows; 33.2% had low weight, 44.3% normal, 10.8% overweight and 11.7% obese. 14.8% of boys and 8.6% of girls, 13.5% of the 4th graders, 12.1% of the 5th graders and 9.2% of the 6th graders, and 13.7% of urban students and 9.6% of rural students were obese. 2. According to general characteristics and obesity of the subjects, there were significant differences by sex(${\chi}^2=29.55$, p= .000), grade(${\chi}^2=12.84$, p= .046), region(${\chi}^2=41.96$, p= .000) and pocket money(${\chi}^2=33.43$, p= .001). 3. As genetic factors, all variables including sibling's obesity r= .128(p= .000), mother's obesity r= .098(p= .000) and father's obesity r= .081(p= .001) had influence on children's obesity. 4. Concerning environmental factors, there was positive correlation between eating habits r=- .052(p= .02) and school record r=- .048(p= .04) and there was negative correlation between internet using time r= .050(p= .03) and conflict with parents r= .048(p= .04). 5. Self-respect as psychological factor had negative correlation as r=- .048(p= .04). Conclusion : Consequently, the influential factors on obesity of elementary school students were as follows. Obesity was higher in boys than in girls, in urban areas than in rural areas, in lower grade than in higher grade, in children with obese siblings, obese father and mother, irregular and wrong eating habits, frequent internet uses, parents in conflict and lower school record and self-respect. Therefore, since it was found that these variables were more exact causes to increase children's obesity, development of various obesity mediation programs considering these variables at home, school and society is urgently required.

• 아시안잔디학회지
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• 제23권2호
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• pp.307-316
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• 2009

크리핑벤트그래스는 골프코스의 퍼팅그린에서 주로 사용하고 있는 한지형잔디 초종이다. 크리핑벤트그래스 품종들은 형태적인 특성이나 유전적인 다양성이 협소하여 품종간 구분이 매우 어렵다. 따라서 이들 품종간 유전적인 다양성이나 골프코스 그린의 인터씨딩율에 대한 조사를 위해 SCAR marker를 개발하고자 본 연구를 수행하였다. penncross, penn A-4, crenshaw, L-93, CY-2, T-1 공시품종들에 대한 SCAR marker 개발을 위해 5개 RAPD primer에 대한 품종 간 구분이 가능한 특이적 band를 선발하였다. 선발한 특이적 band의 염기서열을 분석하여 품종별 SCAR primer를 제작하였다. 각 품종의 SCAR primer별로 특이적 band가 나타나는지에 대한 여부를 검정한 결과, penncross, penn A-4, crenshaw 품종의 SCAR primer는 6개 공시품종에서 동일한 크기의 band가 나타나 SCAR marker로써 활용이 어려웠다. 하지만 CY-2의 CY850F/R primer는 850bp, T-1의 T700F/R primer는 700bp, L-93의 L2900F/R는 2.9kb에서 특이적 band가 나타나 3개 품종별 SCAR marker로서 활용이 가능하였다. 따라서 본 연구에서 개발한 3개 품종별 SCAR marker를 활용하여 크리핑벤트그래스의 품종별 유전적 다양 및 골프코스 그린의 인터씨딩율 조사에 활용이 가능할 것으로 기대된다.

• 한국가정과학회지
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• 제9권4호
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• pp.83-93
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• 2006

This study was done for finding out if eating habits, eating behaviors were different between non-obese and obese elementary school students in Jeonju Area. Total 2568 students of 1364 male and 1204 female of the 4th, 5th, and 6th year in 5 elementary schools were surveyed and the statistics of the result was analyzed by SPSS program. The results are summarized as follows: 1. Obesity was defined as Body Mass Index(BMI) that exceeded 85th and Obesity Index(OI) that exceeded 110. First, subjects were divided into 4 groups : lean, normal, overweight and obese. Second subjects were reclassified into non-obese(lean and normal) and obese(overweight and obese) groups. Average height of male and female students were 142.5cm, 143.1cm and weight of those were 36.4kg and 37.9kg respectively. 2. As results of obesity computation, obese male students were 19.6%(overweight 11.3%, obese 8.3%) in BMI and obese male students were 25.0%(overweight 12.5.%, obese 12.5%) in OI. Especially Obesity percent rate of male student were significantly higher on that of female student in OI method. 3. Examining obesity between male and female, there were statistically different between male students and female students in OI, but there were not statistically different in BMI. With regard to grade level(4th, 5th, 6th), there were statistically different among grade levels. 4. Examining correlation between eating habits(eating behaviors) and obesity, there were statistically significant in some cases. For example, there were statistically significant correlation between fast eating habit and obesity. And the relation analysis of general environments and obesity showed that there were statistically significant in some cases. These results suggest that the number of overweight students can be increased due to the amount and kinds of food children have as well as the general causes of overweight such as genetic, environmental and psychological reason. Surveying about children's eating habits, eating behaviors this study methodically. Working with parents is necessary and comparison of eating habits, eating behaviors and nutrition knowledge between the past and their presents are also needed in a future.