• 제목/요약/키워드: General Component Analysis

검색결과 479건 처리시간 0.031초

Comparative Analysis of the Bufonis Venenum by Using TLC, HPLC, and LC-MS for Different Extraction Methods

  • Lee, Hyo-Jae;Koung, Fan-Pei;Kwon, Ki-Rok;Kang, Dae-In;Cohen, Lorenzo;Yang, Pei-Ying;Yoo, Hwa-Seung
    • 대한약침학회지
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    • 제15권4호
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    • pp.52-65
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    • 2012
  • Objectives: Toad venom, called Chan-Su, is a traditional Oriental medicine secreted from the auricular and the skin glands of the Bufo bufo gargarizanz Cantor or B. melanosticus Schneider and has been widely used in China, Korea and other parts of Asia for the treatment of pain, heart conditions, and cancer. We examined the concentrations of the main chemical constituents within a commercially available toad venom product and compared the levels for different extraction methods. Methods: Toad venom was extracted using either cold or hot water, ethanol (EtOH), methanol (MeOH), or ethyl acetate (EtOAc), was fractionated using precipitation or reflux, and was then analyzed using thin layer chromatography (TLC), high-performance liquid chromatography (HTLC), and liquid chroma-tography - mass spectrometry (LC-MS). Individual components were identified by comparisons of the retention times, the ultraviolet spectra, and mass spectras and differences in chemical constituents for different solvents and extraction methods are presented. Results: Components with authentic standards, including serotonin and bufodienolides (cinobufagen, bufalin, cinobufalin, and resibufogenin), were detected. The water extract of toad venom contained the greatest amount of serotonin ($75.7{\pm}0.1$ mg/g), but very small amounts of bufodienolides ($3.8{\pm}0.0$ mg/g). In contrast, the use of MeOH or EtOH extraction solutions resulted in 5-26 times higher concentrations of bufodienolides, with only trace amounts of serotonin. The relative and the absolute concentrations of the component also varied based on the extraction method; i.e., EtOH extracts yielded the greatest total amounts of bufodienolides, and EtOAc precipitation had the lowest amounts of bufodienolides. Conclusions: Toad venom consists of serotonin and several bufodienolides, and the choice of solvent to extract chemical the constituents is important as a way to enrich the purported active components for treating different conditions.

일부 다빈도 입원 및 수술례의 진단과 치료과정에 대한 연구 (An Analysis and Assessment of Diagnostic and Therapeutic Process in Some Frequent Admissions and Operations)

  • 김창엽;김윤;권영대;김용익;신영수;안형식
    • Journal of Preventive Medicine and Public Health
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    • 제26권3호
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    • pp.400-411
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    • 1993
  • The aim of this study is to analyze the variations among hospitals and hospital groups in resource use and procedures of diagnostic and therapeutic process, such as laboratory tests, radiologic examinations, tissue diagnosis, timing of surgery after admission, the time required for operation. The study was performed for five procedures including cesarean section (C/S), appendectomy, cholecystectomy, cataract extraction, and pediatric pneumonia. The 2,316 subjects were selected from medical insurance claims list, and from this list 413 cases were sampled for medical record review. The patterns of resource utilization and process of treatment were described according to hospitals and characteristics of hospital groups. The major results were as follows : 1. The numbers of laboratory and radiologic tests showed significant difference among hospitals and hospital groups. In case of hospital groups, we could find tendencies of more tests with increasing hospital bed size. 2. In general, the proportion of operative cases evaluated by tissue diagnosis postoperatively among all operations ranged from 28.3% to 100%. The proportion varied among hospital groups, of which general hospital A group(more than 15 specialty) showed the highest proportion. 3. Post-admission delay until operation and the time required for operative procedure were not invariable among hospitals and hospital groups. The duration of operation in tertiary hospitals was slightly shorter than general hospitals, with varying statistical significance. We could find that probably there were differences of quality among hospitals in some components of procedures, which suggested that the implementation of quality assurance activities would be mandatory. In this study, we simply described the patterns of resource utilization and some features of clinical process, with institution of the need for advanced studies with in-depth analyses for each component of diagnosis and treatment procedures.

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首都圈地域 小賣業 經營의 空間的 變容 (Spatial Changes in the Business Organization of Retailing in the Seoul Metropolitan Area)

  • 한주성
    • 대한지리학회지
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    • 제31권1호
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    • pp.19-37
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    • 1996
  • 대도시권내 소매업의 지역구조 변화를 이해하기 위하여 우리나라에서 소매업의 규모가 가장 큰 수도권지역 소매업 경영의 지역적 변화형태를 고찰한 결과 다음과 같은 점이 밝혀졌다. 1979~1991년 사이에 연간판매액에 의한 소매업의 업종구성 변화는 <종합 소매업>과 <개인 운수장비 소매업 및 주유소 운영업>의 구성비가 매우 높아졌다. 그리고 소매업의 경영상의 변화는 개인 경영조직이 탁월해지고, 종사자의 常傭化가 많이 나타났다. 주성분 분석에 의한 수도권지역 소매업의 주된 경영성분은 1. 소매업의 대규모화와, 2. <섬유, 의복, 신발 및 의복 악세사리 소매업>, <가구, 철물 및 가정용품 소매업(가전제품 포함)>, <장신구 및 시계 소매업>의 법인 조직화와 상용 조사자의 고용화이다. 소매업 경영의 유형변화를 보면, 1979년에는 섬유, 의복, 신박 및 의복 악세사리 소매업, 가구, 철물 및 가정용품 소매업(가전제품 포함), 장신구 및 시계 소매업의 법인 조직화와 상용 종사자의 고용화, 달리 분류되지 않은 일반 소매업의 대규모 소매업의 법인 조직화가 다소 이루어졌으나, 1991년에는 소매업의 대규모화가 이루어진 것이다. 이러한 경영변화를 나타내는 지구는 서울시와 그 위성도시 및 경기도의 남동부지역이다. 또 1979년에 섬유, 의복, 신발 및 의복 악세사리 소매업, 장신구 및 시계 소매업, 달리 분류되지 않은 일반 소매업의 대규모 소매업의 법인 조직화가 다소 이루어진 것에서, 1991년에는 개인 운수장비 소매업 및 주유소 운영업의 상용 종사자의 고용화가 이루어졌다. 이러한 변화를 나타내는 지구는 경기도의 동부와 북부지역이다. 따라서 우리나라 수도권지역 소매업 경영의 변화형태는 1979년에 일부 소매업이 법인 조직화와 상용 종사자의 고용화가, 1991년에는 소매업의 대규모화와 개인 운수장비 소매업과 주유소 운영업의 종사자 고용화가 나타난 점이다.

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한강하류지형면의 분류와 지형발달에 대한 연구 (양수리에서 능곡까지)

  • 박노식
    • 동굴
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    • 제68호
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    • pp.23-73
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    • 2005
  • Purpose of study; The purpose of this study is specifically classified as two parts. The one is to attempt the chronological annals of Quaternary topographic surface through the study over the formation process of alluvial surfaces in our country, setting forth the alluvial surfaces lower-parts of Han River area, as the basic deposit, and comparing it to the marginal landform surfaces. The other is to attempt the classification of micro morphology based on the and condition premising the land use as a link for the regional development in the lower-parts of Han river area. Reasons why selected the Lower-parts of Han river area as study objects: 1. The change of river course in this area is very serve both in vertical and horizontal sides. With a situation it is very easy to know about the old geography related to the formation process of topography. 2. The component materials of gravel, sand, silt and clay are deposited in this area. Making it the available data, it is possible to consider about not oかy the formation process of topography but alsoon the development history to some extent. 3. The earthen vessel, a fossil shell fish, bone, cnarcoal and sea-weed are included in the alluvial deposition in this area. These can be also valuable data related to the chronological annals. 4. The bottom set conglometate beds is also included in the alluvial deposits. This can be also valuable data related to the research of geomorphological development. 5. Around of this area the medium landform surface, lower landform surface, pediment and basin, are existed, and these enable the comparison between the erosion surfaces and the alluvial surfaces. Approach : 1. Referring to the change of river beds, I have calculated the vertical and horizontal differences comparing the topographic map published in 1916 with that published in 1966 and through the field work 2. In classifying the landform, I have applied the method of micro morphological classification in accordance with the synthetic index based upon the land conditions, and furthermore used the classification method comparing the topographic map published in 1916 and in that of 1966. 3. I have accorded this classification with the classification by mapping through appliying the method of classification in the development history for the field work making the component materials as the available data. 4. I have used the component materials, which were picked up form the outcrop of 10 places and bored at 5 places, as the available data. 5. I have referred to Hydrological survey data of the ministry of Construction (since 1916) on the overflow of Han-river, and used geologic map of Seoul metropolitan area. Survey Data, and general map published in 1916 by the Japanese Army Survbey Dept., and map published in 1966 by the Construction Research Laboratory and ROK Army Survey Dept., respectively. Conclusion: 1. Classification of Morphology: I have added the historical consideration for development, making the component materials and fossil as the data, to the typical consideration in accordance with the map of summit level, reliefe and slope distribution. In connection with the erosion surface, I have divided into three classification such as high, medium and low-,level landform surfaces which were classified as high and low level landform surfaces in past. furthermore I have divided the low level landform surface two parts, namely upper-parts(200-300m) and bellow-parts(${\pm}100m$). Accordingly, we can recognize the three-parts of erosion surface including the medium level landform surface (500-600m) in this area. (see table 22). In condition with the alluvial surfaces I have classified as two landform surfaces (old and new) which was regarded as one face in past. Meamwhile, under the premise of land use, the synthetic, micro morphological classification based upon the land condition is as per the draw No. 19-1. This is the quite new method of classification which was at first attempted in this country. 2. I have learned that the change of river was most severe at seeing the river meandering rate from Dangjung-ni to Nanjido. As you seee the table and the vertical and horizontal change of river beds is justly proportionable to the river meandering rate. 3. It can be learned at seeing the analysis of component materials of alluvial deposits that the component from each other by areas, however, in the deposits relationship upper stream, and between upper parts and below parts I couldn't always find out the regular ones. 4. Having earthern vessel, shell bone, fossil charcoal and and seaweeds includen in the component materials such as gravel, clay, sand and silt in Dukso and Songpa deposits area. I have become to attempt the compilation of chronicle as yon see in the table 22. 5. In according to hearing of basemen excavation, the bottom set conglomerate beds of Dukso beds of Dukso-beds is 7m and Songpa-beds is 10m. In according to information of dredger it is approx. 20m in the down stream. 6. Making these two beds as the standard beds, I have compared it to other beds. 7 The coarse sand beds which is covering the clay-beds of Dukso-beds and Nanjidobeds is shown the existence of so-called erosion period which formed the gap among the alluvial deposits of stratum. The former has been proved by the sorting, bedding and roundness which was supplied by the main stream and later by the branch stream, respectively. 8. If the clay-beds of Dukeo-bed and Songpa-bed is called as being transgressive overlap, by the Eustatic movement after glacial age, the bottom set conglomerate beds shall be called as being regressive overlap at the holocene. This has the closest relationship with the basin formation movement of Seoul besides the Eustatic movement. 9. The silt-beds which is the main component of deposits of flood plain, is regarded as being deposited at the Holocene in the comb ceramic and plain pottery ages. This has the closest relationship with the change of river course and river beds.

가죽나무(Ailanthus altissima)의 부위별 성분 분석 (Analysis of Components in the Different Parts of Ailanthus altissima)

  • 이양숙
    • 한국식품저장유통학회지
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    • 제15권2호
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    • pp.261-268
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    • 2008
  • 본 연구는 뿌리를 저근백피라는 한방생약재로 사용하는 가죽나무(Ailanthus altissima)의 뿌리와 줄기 그리고 잎을 기능성 식품을 개발하기 위한 연구의 일환으로 영양정분과 생리활성 물질의 함유량을 측정하였다. 일반성분을 분석한 결과 뿌리에는 조회분(9.20%)과 조단백질(7.86%)이 많았으며, 잎에는 조단백질(11.36%) 그리고 줄기에서는 탄수화물(81.74%)의 함유량이 비교적 높았다. 수용성 단백질은 잎에서 9,839.52 mg%로 뿌리와 줄기보다 매우 많이 함유하였으며, 환윈당과 유리당은 뿌리에서 1,813.94 mg%와 1,140.20 mg%, 잎에서는 1,670.98 mg%와 1,190.42 mg%를 함유하였다. 유리아미노산은 뿌리에서 2,018.58 mg%로 잎(1,070.88 mg%)과 줄기(427.55 mg%)보다 많이 함유하였으며, 특히 뿌리에는 arginine과aspartic acid의 함유량이 매우 높아 유리아미노산의 약 85%를 차지하였다. 아미노산 유도체 함량은 잎에서 780.70 mg%로 가장 높았으며, 뿌리는 430.95 mg%을 함유하였다. 이중 뿌리에는 항산화 및 간기능 향상에 효과가 있는 것으로 알려진 taurine을 61.68 mg% 함유하였다. 가죽나무의 부위별 무기질을 분석한 결과 모든 부위에서 Ca와 K 그리고 Mg의 함유량이 매우 높아 무기질 총량의 $83{\sim}98%$를 차지하였으며, 뿌리에는 Fe와 Al의 함량도 비교적 높았다. 가죽나무의 부위별 시료와 추출물에 대한 폴리페놀과 플라보노이드 화합물을 측정한 결과, 잎에는 821.58 mg%의 폴리페놀과 2,501.67 mg%의 플라보노이드를 함유하였으며, 추출물 또한 잎의 물 추출물에서 8,040.35 mg%의 폴리페놀과 에탄을 추출물에서 13,592.20mg%의 플라보노이드를 함유하였다. 이상의 결과 한방생약재로 사용되고 있는 가죽나무의 뿌리를 포함하여 잎에서도 영양성분과 생리활성 물질이 다량 함유된 것으로 분석되었다.

한국에서 재배되는 상품종별 영양가치 분석 (The nutritive value analysis of the mulberry cultured in Korea)

  • 최병희;남중희
    • 한국잠사곤충학회지
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    • 제2권
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    • pp.73-81
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    • 1962
  • This treatise is to analyze the chemical components in the mulberry varieties which are cultured in Korea and to estimate the nutritive values for them. Some of these varieties were developed lately in this country just by the general breeding aspect of mulberry leaf producing and silkworm raising experimentation without analysing the nutritive values of them which may be differed by various culturing condition. This work will be helpful for the improvement of mulberry varieties and the correct judgement of them. The varieties used for the analysis are as followings; A. Morus bombycis Koridz species. 1.Ichi-Hei. 2. Shimano-Uchi B. Morus alba L. species. 3. Kairyo-Nezumigaeshi. 4. Suwon-Daeyop. 5. Suwon No. 3. 6. Suwon No. 4. 7. Yongchon-Chuwoo. C. Morus Ihou (Ser.) Koidz species. 8. Ro-Soh. The specimens were eventually taken under the air and soil dry season because of the rain shortage during the sampling period and the results are found as a rather different from the normal specimen under the normal climate. Therefore, this treatise will be an important report on a special culturing condition and the nutritive values by the mulberry varieties, and still comparable because they were grown under the same condition. The individual conclusions are; 1. Yongchon-Chuwoo which was originated in this country, was found as an important variety for this country from the aspect of mulberry nutritive value and leaf producing amount, and it is believed that this is the best suitable variety for the soil of Korea. 2. Ichi-Hei occupies the better situation as well as Yongchon-Chuwoo from the nutritive criticizing even though it was originated in Japan. 3. The lately developed Suwon No.3 and No.4 are best from the leaf producing point of view, but they are not found such a good varieties from the nutritive aspect. The result may be happened as poor because they were cut before spring sprout started. 4. Ro-Soh which was normally recognized as a poor variety, was also found as a poor nutritive and leaf producing variety. 5. The crude protein and crude fat components in the mulberry leaves decreased as the leaf maturity was progressed, but the crude fiber and crude ash components increased reversively in general view. It was also found that there were some specific changing nature by the mulberry varieties and could not rule them by just one conclusion. 6. During the air and soil dry season, the ash component increases but the hydrocarbon and moisture contents decrease considerably, and which resulted to the increase of mulberry hardness ratio and the decrease of sugar-protein ratio, eventually it causes the decrease of the nutritive value. It was also found that Ichi-Hei, Ro-Soh, and Suwon No. 3 had a strong recovering nature to their normal nutritive condition after raining. 7. Mulberry is of course a rich calorie diet, and is calculated as 50 to 60 Cal. per gram.

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성인지적 교수전략을 활용한 수업실태 분석 및 효과 연구 (Studies of the Analysis of Class Condition and the Effects of Instruction Using Gender-Sensitive Teaching Strategies)

  • 홍경선;김동익;구수연;안진경
    • 공학교육연구
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    • 제13권3호
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    • pp.34-52
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    • 2010
  • 본 논문에서는 공과대학에서 성인지적 교수전략을 활용한 수업실태를 분석하였고, 성인지적 교수전략을 활용한 수업이 공과대학 학생의 수업 만족도와 자기주도성에 미치는 효과를 검증하였다. 성인지적 교수전략을 활용한 수업실태를 분석한 결과, 거의 모든 항목에서 실험집단의 평균이 통제집단의 평균보다 높은 편이었다. 성인지적 교수전략을 활용한 수업의 효과를 검증한 결과, 성 인지적 교수전략을 활용한 수업은 전체 실험집단 공대학생의 수업 만족도에 통계적으로 긍정적인 영향을 미치지않는 것으로 나타났지만, 실험집단 가운데 B1 실험집단의 수업 만족도에 통계적으로 긍정적인 영향을 미치는 것으로 나타났다. 한편, 성인지적 교수전략을 활용한 수업은 실험집단 공대학생의 자기주도성 하위요인인 학습전략적 측면과 실험집단 공대남학생의 자기주도성 총점에 긍정적인 영향을 미치는 것으로 나타났다. 특히, 실험집단인 B1집단 공대학생과 공대남학생의 자기주도성에 긍정적인 영향을 미쳤다.

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남성 소비자의 미용실 이용 실태와 서비스 품질이 만족도 및 충성도에 미치는 영향 (A Study on the Actual Condition and Service Quality of Men's Consumers' Use of Hairdressing Room)

  • 리순화;유선희;정다운
    • 한국응용과학기술학회지
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    • 제36권1호
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    • pp.90-101
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    • 2019
  • 본 연구는 남성 시술자를 통해 관리 받은 남성 고객을 대상으로 일반 미용실과 남성 미용실에 대한 이용 실태 비교와 서비스 품질 인식이 만족도 및 충성도에 미치는 영향을 확인하고자 하였다. 총 405명의 설문지를 분석 자료로 사용 하였으며, 측정 도구의 신뢰도 및 타당성을 검증하기 위해 주성 분석을 이용하였다. 서비스 품질 인식의 하위요인으로는 전문성, 친화성, 신뢰성, 지속성을 도출하였고, 만족도 및 충성도의 타당성 및 신뢰도 검증을 실시하였다. 연구 결과, 일반 미용실과 남성 전문 미용실의 헤어 관여도, 서비스 품질 인식, 만족도 및 충성도에 대한 차이가 확인되었으며, 서비스 품질 인식의 하위요인별 전문성, 친화성, 신뢰성, 지속성은 만족도, 충성도와 통계적으로 유의미한 정(+)의 상관관계가 확인되었다(p<0.01). 또한, 남성 전문 미용실은 서비스 품질 인식이 만족도와 충성도에 정(+)의 영향을 미치며, 서비스 품질 만족도는 충성도에 정(+)을 미치는 것으로 확인되었다. 본 연구를 통하여 남성들의 뷰티 시장에 중요한 마케팅 기초 자료로써 활용 가능성이 있음이 사료되어 진다.

RNA 시퀀싱 기법으로 생성된 빅데이터 분석 (Big Data Analytics in RNA-sequencing)

  • 우성훈;정병출
    • 대한임상검사과학회지
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    • 제55권4호
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    • pp.235-243
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    • 2023
  • 차세대 염기서열 분석이 개발되고 널리 사용됨에 따라 RNA-시퀀싱(RNA-sequencing, RNA-seq)이 글로벌 전사체 프로파일링을 검증하기 위한 도구의 첫번째 선택으로 급부상하게 되었다. RNA-seq의 상당한 발전으로 다양한 유형의 RNA-seq가 생물정보학(bioinformatics) 발전과 함께 진화했으나, 다양한 RNA-seq 기법 및 생물정보학에 대한 전반적인 이해 없이는 RNA-seq의 복잡한 데이터를 해석하여 생물학적 의미를 도출하기는 어렵다. 이와 관련하여 본 리뷰에서는 RNA-seq의 두 가지 주요 섹션을 논의하고 있다. 첫째, Standard RNA-seq과 주요하게 자주 사용되는 두 가지 RNA-seq variant method를 비교하였다. 이 비교는 어떤 RNA-seq 방법이 연구 목적에 가장 적절한지에 대한 시사점을 제공한다. 둘째, 가장 널리 사용되는 RNA-seq에서 생성된 데이터 분석; (1) 탐색적 자료 분석 및 (2) enriched pathway 분석에 대해 논의하였다. 데이터 세트의 전반적인 추세를 제공할 수 있는 주 성분 분석, Heatmap 및 Volcano plot과 같이 RNA-seq에 대해 가장 널리 사용되는 탐색적 자료 분석을 소개하였다. Enriched pathway 분석 섹션에서는 3가지 세대의 enriched pathway 분석에 대해 소개하고 각 세대가 어떤 식으로 RNA-seq 데이터 세트로부터 enriched pathway를 도출하는지를 소개하였다.

인삼(人蔘)사포닌의 동물행동(動物行動)에 대(對)한 작용(作用) (The Effects of Ginseng Saponin on Animal Behavior)

  • 홍사악;박찬웅;김재훈;홍순근;장현갑;김명석
    • 대한약리학회지
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    • 제10권2호
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    • pp.1-11
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    • 1974
  • Results of an experiment on the behavior of rats and mice in order to explore the possible pharmacological actions of Panax ginseng upon the central nervous system can be summarized as follows: 1. Spontaneous motor activity. In the case of mice, those groups who were administered 2.5 mg and 5.0mg of ginseng saponin per kilogram of body weight were observed to have increased their activity compared with the control group, while the 50.0 mg and 100.0 mg per kilogram body weight groups demonstrated lower levels of activity, with the peak of activity appearing at 30 minutes after administration of drugs. In the case of rats, those groups of animals who were given injections in the dosage of 2.5 mg, 5.0 mg and 50.0 mg per kilogram body weight demonstrated higher activity than the control group, while the 100.0 mg per kilogram group appeared to have decreased in their activity, with the peak action appearing 30 minutes after the administration of ginseng saponin. The 50.0 mg per kilogram group demonstrated no significant differential. 2. General behavior analysis. In the case of mice, decrease in sleeping component of behavior and increase in the walking and roaring components, compared those with the control group, turned out to be a common phenomenon among the groups who were administered 2.5 mg, 5.0 mg and 50.0 mg of ginseng saponin per kilogram body weight, with the 5.0 mg per kilogram group standing out of all the other groups in terms of their reactions. In the case of rats, ginseng saponin appeared to reduce sleeping component with 2.5 mg, 5.0 mg and 50.0 mg per kilogram body weight groups, while increased the walking and rearing components. It was observed that administratoin of ginseng saponin in a dose of 2.5 mg per kilogram appeared to markedly increase the lying and grooming components of animal behavior. 3. Open-field exploratory behavior. Adminstration of ginseng saponin to mice in doses of 5.0 mg, 50.0 mg and 100.0 mg per kilogram body weight decreased activity, but increased their exploratory behavior. In the case of rats, however, administration of ginseng saponin in the doses of 2.5 mg and 5.0 mg per kilogram body weight markedly increased their activities, while decreased activities with the 50.0 mg per kilogram and 100.0 mg per kilogram groups. The exploratory behavior of rats appeared to have decreased, while grooming increased ramarkably. 4. The above findings from a series of experiment appear to suggest a stimulating effect on the central nervous system when ginseng saponin is administered in small doses, but that larger doses might result in an inhibitory effect, though differential results can be anticipated with modification of experimental conditions.

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