• 한국육종학회지
• /
• 제42권2호
• /
• pp.188-194
• /
• 2010

시클라멘의 꽃 및 꽃눈의 색, 잎의 모양과 무늬 등의 질적 형질에 대한 유전 분석을 위하여 이들의 특성이 다른 계통을 교잡한 후, $F_1$, $F_2$ 및 여교잡 세대에서 분리비를 조사하였다. 화색은 $F_1$이 양친의 중간색을 나타냈고, $F_2$는 자방친 화색: 중간 색($F_1$과 동일): 화분친 화색이 1: 2 : 1로 분리되어 한 쌍의 대립유전자가 불완전우성으로 유전하는 것으로 나타났다. 꽃눈 색은 흰색 ${\times}$ 빨간색 계통의 조합에서 $F_1$이 빨간색으로 나타났고, $F_2$에서 빨간색 3: 흰색 1로 분리되어 한 쌍의 대립유전자가 우성으로 작용하는 것으로 밝혀졌다. 모인 꽃잎 형태는 흐트러진 꽃잎 형태에 대하여 완전우성으로 유전하는 것으로 나타났다. 잎 형질에 있어서는 넓은 은색 무늬 잎이 선형 무늬 잎에 대하여, 그리고 피침형 잎 끝이 타원형 잎 끝에 대하여 각각 우성으로 작용하는 한 쌍의 대립유전자가 관여하는 것으로 나타났다. 이와 같이 시클라멘의 질적형질의 발현에는 한 쌍의 대립 유전자가 작용하며 꽃의 색은 불완전우성으로, 꽃눈의 색, 잎의 형태 및 무늬는 완전우성으로 유전되는 것으로 밝혀졌다.

• Fisheries and Aquatic Sciences
• /
• 제18권1호
• /
• pp.99-107
• /
• 2015

A natural hybrid of a probable intergeneric mating between the striped shiner Pungtungia herzi and the stone morocco Pseudorasbora parva (Cypriniformes: Cyprinidae) was captured in the Geumho River, a tributary of the Nakdong River basin in Korea. Morphological characters and DNA sequences were analyzed to verify its hybrid state and identify the parentage of its parent species. The hybrid exhibited a phenotypic intermediacy between the two parent species in the number of vertebrae and the mouth shape. Out of 1,488 base pair (bp) positions of the nuclear recombination activating gene 1 gene (rag1), which has a biparental mode of inheritance, 41-bp substitutions were detected between the two parent species, whereas an electropherogram of the hybrid displayed polymorphic double peaks at all of the base positions, along with one additional one, strongly indicating its hybrid state. Meanwhile, sequence comparison of the mitochondrial cytochrome b gene (mt-cyb) (1,140 bp), which has a maternal mode of inheritance, showed only 5-22-bp differences (97.6-99.5% identities) between the hybrid and Pu. herzi, but as many as 158-168-bp differences (85.2-86.1% identities) between the hybrid and Ps. parva, clearly indicating Pu. herzi as the maternal species. Thus, combined analyses of independent data sets (i.e., morphology and nuclear and mitochondrial DNA sequences) offered convincing evidence for the hybrid state of a naturally occurring hybrid resulting from intergeneric mating between a female Pu. herzi and a male Ps. parva.

• 한국연초학회지
• /
• 제9권1호
• /
• pp.45-55
• /
• 1987

For some basic information in tobacco breeding, the modes of inheritance and heritabilities for the twelve agronomic and chemical characters were estimated in the study of eight varieties partial diallel set. Additive gene actions were significant for all characters except total nitrogen content and dominance gene effects were also significant for all characters. Yield, number of leaves per plant, leaf length and leaf shape index were inherited as partial dominance, and overdominance was detected for plant height, stem diameter, internode length, total alkaloid, total nitrogen and total sugar content. Dominant gene showed increasing effects in yield, plant height, stem diameter, internode length, leaf width and total sugar content, but showed decreasing effects in the other characters. The broad heritability was very high for all characters. and the narrow heritability was high in days to flower and yield, but low in internode length, total alkaloid, total nitrogen and total sugar content.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제2권1호
• /
• pp.79-81
• /
• 2001

A study was conducted to establish the degree of genetic divergence between different hybrid forms and rearing conditions through estimation of the minimum number of genes (allelic pairs) differentiating parents in terms of specific quantitative traits. It was established that the minimum gene numbers differentiating parental lines in the inheritance of cocoon was 1, of cocoon shell weight- between 1 and 2, and of silk filament length- between 2 and 3. The variability in the specific genetic parameter could be explained by the reliability of the statistical-and-genetic method used and the expression of genes affecting the formation of each of the characters tested. Gene expression, in its turns is conditioned both by the gene interaction within the genotypes and the different genotype response to environmental change. To go deep in the problem, experiments should be conducted under strictly controlled conditions, reducing the mathematical-and-genetic analysis to a physiological levels and hence to analyse the genetic nature of the specific quantitative character formation and its genetic control.

• 한국연초학회지
• /
• 제4권1호
• /
• pp.15-20
• /
• 1982

This experiment was carried out to investigate the degrees and directions of dominance, and gene distributions by analysing diallel crosses of oriental varieties. The analysis of Wr-Vr indicated non-allelic gene interaction for days to (lowering and number of leaves. Five Plant characteristics showed different degrees of dominance : incomplete dominance for Plant height and leaf shape, over dominance for , wield and sugar, and complete dominance for nicotine. It was shown that additive genetic variance was predominant for plant height, leaf shape, and total sugar. More number of dominant genes were present in Kavala and canthi for Plant height; Basma, Samsun and Izmir for leaf shape ; and Basma and Samsun (or nicotine. Their directions of dominance were tall height, narrow leaves, and low nicotine, respectively.

• 한국연초학회지
• /
• 제17권2호
• /
• pp.114-119
• /
• 1995

Cultivars of Nicotiana tabacum L. normally have pink flowers, but the flue-cured tobacco mutant line, BU 8832-85, had white flower. The mutant line was crossed with five normal varieties of KF 109, NC 82, TC 499, NC 567 and Coker 176. All Fl plants showed pink flower. The progenies of F2 generations were segregated with the phenotypic ratio 9 : 3 : 4 with pink, varigated(a recombinant type) and white flower, respectively. Test-cross populations showed 1 : 1 : 2 ratios. These results showed that the white flower character was controlled by two recessive genes. The genes were designated as FFCC for pink and ffcc for white flower. The recessive gene ff was epistatic to C and c. Therefore, white flower had a recessive epistasis gene.

• 식물조직배양학회지
• /
• 제25권2호
• /
• pp.131-134
• /
• 1998

Antisense PG 유전자 형질전판 토마토로부터 자식시켜 얻은 종자를 kanamycin 내성을 이용하여 5세대까지 분리, 육성하여 antisense PG 유전자가 안정적으로 고정된 식물체를 얻었다. 이들 식물체에는 genomic DNA gel blot 분석으로 antisense PG 유전자가 안정적으로 유전되며 northern blot 분석을 통하여 antisense RNA가 발현됨을 확인하였고, 또한 antisense RNA에 의한 endogenous PG 유전자의 발현 저해를 분석하였다. Antisense PG 유전자 형질전환 성숙 토마토내 PG 효소 활성이 비형질전환 성숙 토마토에 비하여 37-65% 수준으로 저하되었다.

• Journal of Genetic Medicine
• /
• 제15권2호
• /
• pp.97-101
• /
• 2018

Tricho-rhino-phalangeal syndrome (TRPS) is a hereditary disorder characterized by craniofacial and skeletal abnormalities. A mutation of the TRPS1 gene leads to TRPS type I or type III. A 20-year-old male patient visited our neurologic department with chronic fatigue. He presented with short stature, sparse hair, pear-shaped nose, and brachydactyly. Radiologic study showed short metacarpals, metatarsals with cone-shaped epiphyses, hypoplastic femur and hip joint. Panel sequencing for OMIM (Online Mendelian Inheritance in Man) listed genes revealed a de novo heterozygous frameshift mutation of c.1801_1802delGA (p.Arg601Lysfs*3) of exon 4 of the TRPS1 gene. The diagnosis of TRPS can be challenging due to the rarity and variable phenotype of the disease, clinicians should be aware of its characteristic clinical features that will lead a higher rate of diagnosis.

• Applied Biological Chemistry
• /
• 제41권2호
• /
• pp.137-140
• /
• 1998

딱정 벌레목 유충에 살충성을 나타내는 형질 전환 토마토 식물체를 4세대까지 분자 육종을 하였다. 분자 육종된 4세대 식물체에서 살충성 유전자인 B.t.t. 독소 유전자와 유전자가 발현되는 것을 확인하였다. 이 4세대 형질 전환 식물체는 독소 유전자 발현에 의해 딱정 벌레 유충에 살충성을 나타내고 있음이 확인 되었고, 4세대 형질 전환 식물체는 염색체는 2 배수체로 확인되어 정상적인 토마토 식물체임이 증명 되었다. 이 결과들은 형질 전환에 사용된 독소 유전자가 다음 세대로 안정되게 유전되고 있음을 나타내고 있다는 증거이다. 이러한 형질 전환된 식물체의 분자 육종은 농업에 있어서 현재의 일시적인 해충 방제에 비해 장기적인 해충 구제에 대한 새로운 방법의 가능성을 제시하고 있다고 하겠다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2000년도 추계 학술대회지
• /
• pp.202-203
• /
• 2000