Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Inheritance and Expression of Antisense Polygalacturonase Gene in Transgenic Tomato

Antisense Polygalacturonase 유전자 형질전환 토마토의 후대 발현 분석

  • 김영미 (농촌진흥청 농업과학기술원 생물자원부 생화학과) ;
  • 한장호 (농촌진흥청 농업과학기술원 생물자원부 생화학과) ;
  • 김용환 (농촌진흥청 농업과학기술원 생물자원부 생화학과) ;
  • 이성곤 (농촌진흥청 농업과학기술원 생물자원부 생화학과) ;
  • 황영수 (농촌진흥청 농업과학기술원 생물자원부 생화학과)
  • Published : 1998.03.01
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Abstract

$\textrm{T}_{5}$ progeny of one transgenic tomato line (To9) carrying antisense polygalacturonase (PG) cDNA was generated by selfing. Five $\textrm{T}_{5}$ plants were used to analyse in detail. The PG antisense gene was stably inherited through fifth generations. In all five $\textrm{T}_{5}$ plants, expression of the antisense transcripts were detected. In consequence, it led to a reduction of the PG enzyme activity in ripe fruit to between 37% and 65% that of normal. In two plants the expression of endogenous PG gene was inhibited in ripe fruit.

Antisense PG 유전자 형질전판 토마토로부터 자식시켜 얻은 종자를 kanamycin 내성을 이용하여 5세대까지 분리, 육성하여 antisense PG 유전자가 안정적으로 고정된 식물체를 얻었다. 이들 식물체에는 genomic DNA gel blot 분석으로 antisense PG 유전자가 안정적으로 유전되며 northern blot 분석을 통하여 antisense RNA가 발현됨을 확인하였고, 또한 antisense RNA에 의한 endogenous PG 유전자의 발현 저해를 분석하였다. Antisense PG 유전자 형질전환 성숙 토마토내 PG 효소 활성이 비형질전환 성숙 토마토에 비하여 37-65% 수준으로 저하되었다.

Keywords

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