• 대한전기학회논문지:시스템및제어부문D
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• 제53권8호
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• pp.578-584
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• 2004

The CSVM(Current Support Vector Machine) that is a digital architecture performing all phases of recognition process including kernel computing, learning, and recall of SVM(Support Vector Machine) on a chip is proposed. Concurrent operation by parallel architecture of elements generates high speed and throughput. The classification problems of bio data having high dimension are solved fast and easily using the CSVM. Quadratic programming in original SVM learning algorithm is not suitable for hardware implementation, due to its complexity and large memory consumption. Hardware-friendly SVM learning algorithms, kernel adatron and kernel perceptron, are embedded on a chip. Experiments on fixed-point algorithm having quantization error are performed and their results are compared with floating-point algorithm. CSVM implemented on FPGA chip generates fast and accurate results on high dimensional cancer data.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제53권5호
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• pp.286-292
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• 2004

In this study, an integrated microelectrode array was fabricated on glass slide using microfabrication technology. Probe DNAs consisting of mercaptohexyl moiety at their 5-end were spotted on the gold electrode using micropipette or DNA arrayer utilizing the affinity between gold and sulfur. Cyclic voltammetry in 5mM ferricyanide/ferrocyanide solution at 100 ㎷/s confirmed the immobilization of probe DNA on the gold electrodes. When several DNAs were detected electrochemically, there was a difference between target DNA and control DNA in the anodic peak current values. It was derived from specific binding of Hoechst 33258 to the double stranded DNA due to hybridization of target DNA. It suggested that this DNA chip could recognize the sequence specific genes. It suggested that multichannel electrochemical DNA microarray is useful to develop a portable device for clinical gene diagnostic System.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 추계학술대회
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• pp.155-156
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• 2003

RNA was isolated from mucosal tissue of the duodenum, jejunum, ileum and colon after perfusion. The gene expression profiles were measured using Affymetrix GeneChip(R) analysis. Prodrug valacyclovir permeability was 5-fold and 2-fold higher than the parent drug acyclovir in the jejunum and ileum, respectively.(omitted)

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2001년도 가을 학술발표논문집 Vol.28 No.2 (1)
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• pp.598-600
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• 2001

DNA chip technology enables us to monitor thousands of gene expressions per sample simultaneously. Typically, DNA microarray data has at least several thousands of variables (genes) wish relatively smal1 number of samples. Thus feature (gene) selection by dimensionality reduction is necessary for efficient data analysis. In this paper we employ the partial least squares (PLS) method for gene selection and the principal component analysis (PCA) method for classification. The useful behavior of the PLS is verified by computer simulations.

• 한국환경과학회지
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• 제24권5호
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• pp.623-631
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• 2015

Microarray technology provides a unique tool for the determination of gene expression at the level of messenger RNA (mRNA). This study, the mRNA expression profiles provide insight into the mechanism of action of cadmium in Fleshy shrimp (Fenneropenaeus chinensis). The ability of genomic technologies was contributed decisively to development of new molecular biomarkers and to the determination of new possible gene targets. Also, it can be approach for monitoring of trace metal using oligo-chip microarray-based in potential model marine user level organisms. 15K oligo-chip for F. chinensis that include mostly unique sets of genes from cDNA sequences was developed. A total of 13,971 spots (1,181 mRNAs up- regulated and 996 down regulated) were identified to be significantly expressed on microarray by hierarchical clustering of genes after exposure to cadmium for different conditions (Cd24-5000 and Cd48-1000). Most of the changes of mRNA expression were observed at the long time and low concentration exposure of Cd48-1000. But, gene ontology analysis (GO annotation) were no significant different between experiments groups. It was observed that mRNA expression of main genes involved in metabolism, cell component, molecular binding and catalytic function. It was suggested that cadmium inhibited metabolism and growth of F. chinensis.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2003년도 추계학술대회초록집
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• pp.33-33
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• 2003

There prevalence of $\beta$-lactamases bacteria in animals has been increased since 1990s [1]. The resistance in E coli which is mediated by $\beta$-lactamases hydrolyze the $\beta$-lactam ring eventually inactivate the antibiotics [2]. Generally, $\beta$-lactamases can be classified into four main groups and eight subgroups according to their functional and structural characteristics [3]. The detection of $\beta$-lactam antibiotic-resistant bacteria by DNA chip has been described [4]. The chip has a specific probe DNAs that contained the $\beta$-lactam antibiotic-resistant genes which was labeled by multiplex PCR reaction with a mixture of primer sets that were designed to amplify specific gene. Here we report the susceptibility of enteropathogenic E. coli isolated from pigs in Korea using the DNA chip in detecting $\beta$-lactam antibiotic-resistant genes. (omitted)

• 대한기계학회논문집A
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• 제30권10호
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• pp.1261-1268
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• 2006

This paper reports low-cost microreactor $(10{\mu}{\ell})$ biochip for the DNA PCR (polymerase chain reaction). The microbiochip $(20mm{\times}28mm)$ is a hybrid type which is composed of PDMS (polydimethylsiloxane) layer with serpentine micochannel $(360{\mu}m{\times}100{\mu}m)$ chamber and glass substrate integrated with microheater and thermal microsensor. Undesirable bubble is usually created during sample loading to PMDS-based microchip because of hydrophobic chip surface. Created bubbles interrupt stable biochemical reaction. We designed improved microreactor chamber using microfluidic simulation. The designed reactor has a coner-rounded serpentine channel architecture, which enables stable injection into hydrophobic surface using micropipette only. Reactor temperature needed to PCR reaction is controlled within ${\pm}0.5^{\circ}C$ by PID controller of LabVIEW software. It is experimentally confirmed that SRY gene PCR by the fabricated microreactor chip is performed for less than 54 min.

• Tuberculosis and Respiratory Diseases
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• 제55권1호
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• pp.41-58
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• 2003

연구배경 : 약제내성 결핵권의 조기 진단을 위해서, 최근 돌연변이 검출 및 질병의 진단 등에 새로운 기술로 대두되고 있는 올리고뉴콜레오티드 칩 기술을 이용하여 결핵균의 리팜핀, 아이소나아지드와 스트렙토마이신 내성과 관련된 rpoB, katG와 rpsL 유전자의 주요 돌연변이를 신속하고 정확하게 검출하고자 하였다. 방 법 : 리팜핀 내성 검출의 야생형 7개와 돌연변이형 13개, 아이소니아지드 내성 검출의 야생형 2개와 돌연변이형 3개, 그리고 스트렙토마이신 내성 검출을 위한 야생형과 돌연변이형 프로브 각 2종류를 고안한 후, 유리 슬라이드에 고정시켜 올리고뉴클레오티드 칩을 제작하였고, 약제내성을 가지고 있는 배양균주 55균주를 선택하여 PCR 증폭반응과 혼성화 반응을 실시한 후 비공초점 레이저 스케너를 이용하여 돌연변이를 검출하였다. 이를 염기서열방법으로 확인하여 돌연변이 다형성을 분석하였다. 결 과 : 리팜핀 내성은 코돈 531과 코돈 526에서 65%의 돌연변이를 검출하였고, 현재까지 보고되어 있지 않은 D516F의 새로운 돌연변이도 검출하였다. 아이소니아지드 내성은 S315T와 R463L 돌연변이가 45.2%로 검출되었고, 스트렙토마이신 내성은 K43R과 K88R 돌연변이가 78%로 검출되었다. 리팜핀 내성의 88%(35/40), 아이소니아지드 내성의 50%(20/42), 그리고 스트렙토마이신 내성의 78%(7/9)를 검출함으로써 현재까지 보고되어 있는 세가지 약제내성과 관련된 rpoB, katG와 rpsL 유전자의 주요 돌연변이는 대부분 검출할 수 있음을 확인할 수 있었고, 염기서열분석 결과와 비교하였을 때 모두 일치하는 결과를 얻음으로써 올리고뉴콜레오티드 칩의 유용성을 확인할 수 있었다. 결 론 : 따라서 본 연구에서 개발한 올라고뉴클레오티드 칩은 약재내성 결핵균의 조기 진단에 유용한 도구가 될 것으로 사료된다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.688-688
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• 2003

• 동의생리병리학회지
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• 제18권5호
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• pp.1291-1300
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• 2004

Baicalin, a biologically active flavonoid form the roots of Scutallaria baicalensis (Skullcap), have been reported to not only function as anti-oxidants but also cause anticancer effect. We investigated the mechanism of baicalin-induced cytotoxicity and the macro scale gene expression analysis in leukemia cell line, HL-60 cells. Baicalin (10 μM) were used to treat the cells for 6h, 12h, 24h, 48h and 72h. In a human cDNAchip study of 65,000 genes evaluated 6, 12, 24, 48. 72 hours after treated with Baicalin in HL-60 cells. Hierarchical cluster against the genes which showed expression changes by more than two fold. One hundred one genes were grouped into 6 clusters according to their profile of expression by a hierarchical clustering algorithm. For genes differentially expressed in response to baicalin treatment, we tested functional classes based on Gene Ontology (GO) terms. This study provides the most comprehensive available survey of gene expression changes in response to baicalin treatment in HL-60 cell line.