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Studies on Gene Expression of baicalin treated in HL-60 cell line using High-throughput Gene Expression Analysis Techniques  

Kang Bong Joo (Department of Medical Research, KIOM)
Cha Min Ho (Department of Medical Research, KIOM)
Jeon Byung Hun (College of Oriental Medicine, Wonkwang University)
Yun Yong Gab (College of Oriental Medicine, Wonkwang University)
Yoon Yoo Sik (Department of Medical Research, KIOM)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.5, 2004 , pp. 1291-1300 More about this Journal
Abstract
Baicalin, a biologically active flavonoid form the roots of Scutallaria baicalensis (Skullcap), have been reported to not only function as anti-oxidants but also cause anticancer effect. We investigated the mechanism of baicalin-induced cytotoxicity and the macro scale gene expression analysis in leukemia cell line, HL-60 cells. Baicalin (10 μM) were used to treat the cells for 6h, 12h, 24h, 48h and 72h. In a human cDNAchip study of 65,000 genes evaluated 6, 12, 24, 48. 72 hours after treated with Baicalin in HL-60 cells. Hierarchical cluster against the genes which showed expression changes by more than two fold. One hundred one genes were grouped into 6 clusters according to their profile of expression by a hierarchical clustering algorithm. For genes differentially expressed in response to baicalin treatment, we tested functional classes based on Gene Ontology (GO) terms. This study provides the most comprehensive available survey of gene expression changes in response to baicalin treatment in HL-60 cell line.
Keywords
Yukmijihwang-tang; DNA chip; gene expression; pathway analysis;
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  • Reference
1 Apoptosis of HIV-infected cells following treatment with Sho-Saiko-to and its components /
[ Wu X;Akatsu H;Okada H. ] / Jpn J Med Sci Biol.
2 Baicalin 함유생약의 제제화 및 생체이용률 (제1보) : 황금 및 황련 공침물의 확인 및 물리화학적 성질 /
[ 양재헌;김동수;박현구;이남희 ] / J. Kor. Pham. Sci.
3 Baicalin induces apoptosis via mitochondrial pathway as prooxidant /
[ Ueda S;Nakamura H;Masutani H;Sasada T;Takabayashi A;Yamaoka Y;Yodoi J. ] / Mol Immunol.   ScienceOn
4 Protective activity of adult T cell leukemia-derived factor (ADF) against tumor necrosis factor-dependent cytotoxicity on U937 cells /
[ Matsuda M;Masutani H;Nakamura H;Miyajima S;Yamauchi A;Yonehara S;Uchida A;Irimajiri K;Horiuchi A;Yodoi J. ] / J Immunol.
5 Anticancer, antiradical and antioxidative actions of novel Antoksyd S and its major components, baicalin and baicalein /
[ Ciesielska E;Gwardys A;Metodiewa D. ] / Anticancer Res.
6 Antitumor effects of saikosaponins, baicalin and baicalein on human hepatoma cell lines /
[ Motoo Y;Sawabu N. ] / Cancer Lett.   ScienceOn
7 Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling /
[ Alizadeh AA;Eisen MB;Davis RE;Ma C;Lossos IS;Rosenwald A;Boldrick JC; Sabet H;Tran T;Yu X;Powell JI;Yang L;Marti GE;Moore T;Hudson J Jr;Lu L;Lewis DB;Tibshirani R;Sherlock G;Chan WC;Greiner TC;Weisenburger DD;Armitage JO;Warnke R;Levy R;Wilson W;Grever MR;Byrd JC;Botstein D;Brown PO;Staudt LM. ] / Nature.
8 The transcriptional program in the response of human fibroblasts to serum /
[ Iyer VR;Eisen MB;Ross DT;Schuler G;Moore T;Lee JC;Trent JM;Staudt LM; Hudson J Jr;Boguski MS;Lashkari D;Shalon D;Botstein D;Brown PO. ] / Science
9 Lipoxygenase inhibition induced apoptosis, morphological changes, and carbonic anhydrase expression in human pancreatic cancer cells /
[ Ding XZ;Kuszynski CA;El-Metwally TH;Adrian TE. ] / Biochem Biophys Res Commun.   ScienceOn
10 Baicalin is a major component of PC-SPES which inhibits the proliferation of human cancer cells via apoptosis and cell cycle arrest /
[ Ikezoe T;Chen SS;Heber D;Taguchi H;Koeffler HP. ] / Prostate   ScienceOn
11 DRAKs, novel serine/ threonine kinases related to death-associated protein kinase that trigger apoptosis /
[ Sanjo H.;Kawai T.;Akira S. ] / J. Biol. Chem.
12 The herbal medicine sho-saiko-to inhibits proliferation of cancer cell lines by inducing apoptosis and arrest at the G0/G1 phase /
[ Yano H;Mizoguchi A;Fukuda K;Haramaki M;Ogasawara S;Momosaki S;Kojiro M. ] / Cancer Res.
13 Distinctive gene expression patterns in human mammary epithelial cells and breast cancers /
[ Perou CM;Jeffrey SS;van de Rijn M;Rees CA;Eisen MB;Ross DT; Pergamenschikov A;Williams CF;Zhu SX;Lee JC;Lashkari D;Shalon D;Brown PO;Botstein D ] / Proc Natl Acad Sci U S A.
14 Molecular classification of human carcinomas by use of gene expression signatures /
[ Su AI;Welsh JB;Sapinoso LM;Kern SG;Dimitrov P;Lapp H;Schultz PG;Powell SM;Moskaluk CA;Frierson HF Jr;Hampton GM. ] / Cancer Res.
15 Hogenesch JB.Large-scale analysis of the human and mouse transcriptomes /
[ Su AI;Cooke MP;Ching KA;Hakak Y;Walker JR;Wiltshire T;Orth AP;Vega RG;Sapinoso LM;Moqrich A;Patapoutian A;Hampton GM;Schultz PG ] / Proc Natl Acad Sci U S A.
16 Baicalein and baicalin are potent inhibitors of angiogenesis: Inhibition of endothelial cell proliferation, migration and differentiation /
[ Liu JJ;Huang TS;Cheng WF;Lu FJ. ] / Int J Cancer   ScienceOn
17 Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2 /
[ Iwabuchi K.;Li B.;Massa H.F.;Trask B.J.;Date T.;Fields S. ] / J. Biol. Chem.
18 Induction of apoptosis in prostate cancer cell lines by a flavonoid, baicalin /
[ Chan FL;Choi HL;Chen ZY;Chan PS;Huang Y. ] / Cancer Lett.   ScienceOn
19 Effects of the flavonoid baicalin and its metabolite baicalein on androgen receptor expression, cell cycle progression and apoptosis of prostate cancer cell lines /
[ Chen S;Ruan Q;Bedner E;Deptala A;Wang X;Hsieh TC;Traganos F;Darzynkiewicz Z. ] / Cell Prolif.   ScienceOn
20 Molecular classification of cancer: class discovery and class prediction by gene expression monitoring /
[ Golub TR;Slonim DK;Tamayo P;Huard C;Gaasenbeek M;Mesirov JP;Coller H; Loh ML;Downing JR;Caligiuri MA;Bloomfield CD;Lander ES. ] / Science
21 Systematic variation in gene expression patterns in human cancer cell lines /
[ Ross DT;Scherf U;Eisen MB;Perou CM;Rees C;Spellman P;Iyer V;Jeffrey SS; Van de Rijn M;Waltham M;Pergamenschikov A;Lee JC;Lashkari D;Shalon D;Myers TG;Weinstein JN;Botstein D;Brown PO. ] / Nat Genet.   ScienceOn