The Transactions of the Korean Institute of Electrical Engineers C (대한전기학회논문지:전기물성ㆍ응용부문C)

The Korean Institute of Electrical Engineers (대한전기학회)
권호 표지

Electrochemical Detection of Single Nucleotide Polymorphism (SNP) Using Microelectrode Array on a DNA Chip

미소전극어레이형 DNA칩을 이용한 유전자다형의 전기화학적 검출

  • 최용성 (원광대학교 전기전자 및 정보공학부) ;
  • 권영수 (동아대학교 전기전자 및 컴퓨터 공학부) ;
  • 박대희 (원광대학교 전기전자 및 정보공학부)
  • Published : 2004.05.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, an integrated microelectrode array was fabricated on glass slide using microfabrication technology. Probe DNAs consisting of mercaptohexyl moiety at their 5-end were spotted on the gold electrode using micropipette or DNA arrayer utilizing the affinity between gold and sulfur. Cyclic voltammetry in 5mM ferricyanide/ferrocyanide solution at 100 ㎷/s confirmed the immobilization of probe DNA on the gold electrodes. When several DNAs were detected electrochemically, there was a difference between target DNA and control DNA in the anodic peak current values. It was derived from specific binding of Hoechst 33258 to the double stranded DNA due to hybridization of target DNA. It suggested that this DNA chip could recognize the sequence specific genes. It suggested that multichannel electrochemical DNA microarray is useful to develop a portable device for clinical gene diagnostic System.

Keywords

References

  1. 古莊敏行 他, 「臨床DNA診斷法」, 金原出版, 1995
  2. 江刺正善 他, 「マイクロマシ一ニソグとマイクロメカトロニクス」, 培風館, 1992
  3. T. Livache, B. Fouque, A. Roget, J. Marchand, G. Bidan, R. Teoule, and G. Mathis, 'DNS Chip on a Silicon Device : Example of Hepatitis C Virus Genotyping', Analytical Biochemistry, 255, pp.188-194, 1998 https://doi.org/10.1006/abio.1997.2462
  4. D.Y. Guschin, B.K. Mobarry, D. Proudnikov, D.A. Stahl, B.E. Rittmann, and A.D. Mirzabekov, 'Oligonucleotide Microchips as Genosensors for Determinative and Environmental Studies in Microbiology', Appl Environ Microbiol., 63(6), pp.2397-2402, 1997
  5. S.P.A. Fodor, J.L. Read, M.C. Pirrung, L. Stryer, A.T. Lu, D. Solas, 'Light-directed, spatially addressable parallel chemical synthesis', Science, 251, pp.767-773, 1991 https://doi.org/10.1126/science.1990438
  6. S.P.A. Fodor, R.P. Rava, X.C. Juang, A.C. Pease, C.P. Holmes, C.L. Adams, 'Multiplexed biochemical assays with biological chips', Nature, 364, pp.555-556, 1993 https://doi.org/10.1038/364555a0
  7. M. Schena, D. Shalon, R.W. Davis, P.O. Brown, 'Quantitative monitoring of gene expression patterns with a complementary DNA microarray', Science, 270, pp.467-470, 1995 https://doi.org/10.1126/science.270.5235.467
  8. G. Marrazza, I. Chianclla, M. Mascini, 'Disposable DNA electrochemical sensor for hybridization', detection Biosensors & Bioelectronics, 14, pp.43, 1999 https://doi.org/10.1016/S0956-5663(98)00107-9
  9. S.O. Kelly, E.m. Boon, J.K. Barton, N.M. Kackson and M.G. Hill, 'Single-base mismatch detection based on change transduction through DNA', Nucleic Acids Research, 27, pp.4830-4837, 1999 https://doi.org/10.1093/nar/27.24.4830
  10. E. Palecek, M. Fojta, M. Tomschik, J. Wang, 'Electrochemical biosensors for DNA hybridzation and DNA damage', Biosens & Bioelectron, 13, PP.621-628, 1998 https://doi.org/10.1016/S0956-5663(98)00017-7
  11. J. Wang, X. Cai, G. Rivas, H. Shiraishi, P.A.M. Farias, and N. Dontha, 'DNA Electrochemical Biosensor for the Detection of Short DNA Sequences Related to the Human Immunodeficiency Virus', Anal. Chem., 68, pp.2629, 1996 https://doi.org/10.1021/ac9602433
  12. D.H. Johnson, K.C. Glasgow, and H.H. Thorp, 'Electrochemical Measurement of the Solvent Accessibility of Nucleobases Using Electron Transfer between DNA and Metal Complexes', J. Am. Chem. Soc., 117, pp.8933-8938, 1995 https://doi.org/10.1021/ja00140a006
  13. P.A. Ropp and H.H. Thorp, 'Site-selective electron transfer from purines to electrocatalysts : voltammetric detection of a biologically relevant deletion in hybridized DNA duplexes', Chem Biol., 6, pp.599-605, 1999 https://doi.org/10.1016/S1074-5521(99)80111-2
  14. A.B. Steel, T.M. Herne, and M.J. Tarlov, 'Electrochemical Quantification of DNA Immobilized on Gold', Anal. Chem., 70, pp.4670-4677, 1998 https://doi.org/10.1021/ac980037q
  15. P.N. Gilles, D.J. Wu, C.B. Foster, P.J. Dillon, and S.J. Chanock, 'Single nucleotide polymorphic discrimination by an electronic dot blot assay on semiconductor microchips', Nature Biotechnology, 17, pp.365-370, 1999 https://doi.org/10.1038/7921