• Clinical and Experimental Pediatrics
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• 제56권9호
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• pp.383-388
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• 2013

Purpose: Viral etiology is common in cases of children with acute diarrhea, and antibiotic therapy is usually not required. Therefore, it is important to determine the distribution of common viruses among children hospitalized with acute diarrhea. Methods: We included 186 children who suffered from acute diarrhea and were hospitalized at the Wonkwang University Hospital Pediatric ward from December 1, 2010 to June 30, 2011 in this study. Stool samples were collected and multiplex reverse transcriptase polymerase chain reaction (multiplex RT-PCR) was used to simultaneously determine the viral etiology such as rotavirus, norovirus, astrovirus, or adenovirus. Results: Causative viruses were detected in 72 of the 186 cases (38.7%). The mean age of the virus-positive cases was 1 year and 9 months (range, 1 month to 11 years). Rotavirus was detected in 50/186 (26.9%); norovirus, in 18/186 (9.7%); and astrovirus, in 3/186 cases (1.6%). Adenovirus was not detected in any of the cases. Proportions of norovirus genogroups I and II were 21.1% and 78.9%, respectively. Four of the 51 rotavirus-positive cases (7.8%) had received rotavirus vaccination at least once. The mean duration of diarrhea was 2.8 days (range, 1 to 10 days) and vomiting occurred in 39 of the 72 cases (54.2%). Conclusion: Viral etiology was confirmed in about one-third of the children with acute diarrhea, and the most common viral agent was rotavirus, followed by norovirus.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제4권2호
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• pp.238-242
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• 2001

저자들은 우유 단백질에 대한 FPIES의 1례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.

• Clinical and Experimental Pediatrics
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• 제46권6호
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• pp.602-605
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• 2003

저자들은 salmonella group D로 인한 장염 및 패혈증이 동반된 음낭내 농양을 진단하여 항생제와 절개 및 배농으로 치료한 1례를 경험하였기에 보고하는 바이다.

• 한국미생물·생명공학회지
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• 제47권2호
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• pp.289-295
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• 2019

Acute diarrhea is a global health problem that causes high morbidity and mortality in children. Notably, enteric pathogen co-infections have been suggested to play an important role in gastroenteritis. In this study, we analyzed 1150 stool specimens of patients who visited the pediatric ward of Dongguk University Hospital in Gyeongju province from January 2011 to December 2017. The average isolation rate of potential stool pathogens over 7 years was 37.3% (429/1150), and coinfections were observed in 51 patients (51/429; 11.9%). In the 51 co-infection cases, the most frequent type of co-infection was found to be that of virus-bacteria (33/51). The most frequently detected bacterial pathogen among the co-infected cases was Clostridium spp. (22/51), out of which Clostridium perfringens was found to be the main pathogen (16/22; 72.7%). Escherichia coli spp. were the second most common bacterial pathogens found in 12 cases (12/51; 23.5%), with 10 cases of E. coli EPEC. Furthermore, the most frequently implicated viral pathogen among the co-infected cases was norovirus (16/51), followed by rotavirus (12/51).

• Clinical and Experimental Pediatrics
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• 제48권10호
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• pp.1121-1125
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• 2005

목 적 : 로타바이러스 위장관염은 3개월에서 24개월 사이의 영아들에게 가장 흔하게 발생하는 질환으로 1개월 미만의 신생아에서는 모체에서 태반을 통해 넘어간 IgG의 영향으로 바이러스에 잘 이환되지 않는 것으로 알려져 있다. 그러나 신생아에서 로타바이러스 위장관염에 이환시 괴사성 장염 등의 합병증도 보고된 예가 있다. 이에 저자들은 로타바이러스 위장관염에 이환된 1개월 미만 신생아군이 나타내는 다양한 임상 양상 및 질병의 심각도를 알아보고자 본 연구를 계획하였다. 방 법 : 2002년 6월부터 2004년 7월까지 포천중문의과대학교 분당 차병원에 로타바이러스 위장관염으로 외래를 통해 입원한 1개월 미만 환아 62명을 대상으로 임상 기록지를 후향적으로 분석하였다. 로타바이러스 검사는 stool latex 응집법을 채택하였다. 결 과 : 2년간 로타바이러스 위장관염으로 입원한 환아는 전체 688명이었으며 1개월 미만 환아는 62명(9%)에 해당하였다. 대상 환아의 성별비는 각각 남아 38명(61.3%), 여아 24명(38.7%)이었다. 발생 빈도는 비교적 계절에 관계없이 일정한 발생을 보였으며 특히 3-4월(17명, 27.4%)에 가장 많은 분포를 보였다. 내원 당시 주증상은 원인을 알 수 없는 발열이 가장 많았으며(29명, 49%) 그 다음으로 설사(28명, 45%)와 구토(19명, 30%) 소견을 보였다. 소수의 환아에서는 복부 팽만(4명), 청색증(2명), 경련(2명)을 주소로 내원하였다. 혈액 검사 소견에서는 각각 10명(17%)의 환아들에서 백혈구 수치의 증가와 CRP 양성 소견을 보였으나 이들 환아들에서 더 심한 임상 증세를 보이지는 않았다. 한 명의 환아에서 대변 잠혈 검사 양성 반응을 보였으나 임상적으로 괴사성 장염 증세를 보인 환아는 없었으며 간기능 이상 소견이 한 명에서 보였으나 간비대 소견은 없었다. 입원 환아의 평균 재원 일수는 평균 $5.8{\pm}2.5$일이었다. 환아들의 수유형태는 39명(62.9%)에서 모유 수유였다. 결 론 : 로타바이러스 위장관염에 이환된 환아 중 1개월 미만 신생아는 9%를 차지하며 동반되는 상기도 질환이나 구토없이 발열과 설사만을 주증상으로 하는 경우가 많고 대부분의 경우 보존적인 치료만으로도 증세가 호전된다. 그러나 호발 연령인 3개월에서 24개월 사이의 영아와는 달리 수유량의 감소나 복부 팽만 등 비특이적인 증상만을 보이는 경우가 있으므로 이에 대한 더욱 세심한 관찰과 치료가 필요하다. 또한 분변-경구 경로로 바이러스가 전파되기 때문에 신생아 집단 발병의 위험에 대한 철저한 위생 관리 또한 필요할 것으로 사료된다.

• 대한한방소아과학회지
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• 제23권3호
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• pp.121-131
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• 2009

Objectives This study is to report a case that has an important meaning as a result of treating functional dyspepsia with oriental treatment. We investigated functional dyspepsia in 6 years child who had to continue abdominal distention for a month after gastroenteritis. The child has recovered from all dyspepsia symptoms after treating with oriental medicine. Methods The patient had dyspepsia symptoms all day, especially repeated abdominal distention. He sometimes had nausea, vomiting, diarrhea, abdominal pain. For those symptoms, we treated him with herbal medicine and acupuncture. The aim of treatment was to improve functional dyspepsia without western treatment. We measured patient's abdomen circumference for change of abdominal distention. Results The symptoms of functional dyspepsia were vanished and the patient maintains his condition with oriental medicine treatment without western treatment. After herb medicine treatment and acupuncture treatment the patient's dyspepsia symptoms (nausea, vomiting, diarrhea, abdominal pain, and abdominal distention) were vanished and maintained his condition for two months after complete the treatment. Conclusions This study showed that oriental medicine can elevate the functional dyspepsia children's quality of life with continuous health care and treatment. For more accurate studies, further studies would be needed with more cases.

• Journal of Microbiology and Biotechnology
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• 제30권4호
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• pp.515-525
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• 2020

Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. Lactobacillus plantarum (L. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV), cause high morbidity and mortality in piglets resulting in economic loss. Co-infection by these two viruses is becoming increasingly frequent. Therefore, it is particularly important to develop a new drug to prevent diarrhea infected with mixed viruses in piglets. In this study, we first constructed an anchored expression vector with CWA (C-terminal cell wall anchor) on L. plantarum. Second, we constructed two recombinant L. plantarum strains that anchored IFN-λ3 via pgsA (N-terminal transmembrane anchor) and CWA. Third, we demonstrated that both recombinant strains possess strong antiviral effects against coronavirus infection in the intestinal porcine epithelial cell line J2 (IPEC-J2). However, recombinant L. plantarum with the CWA anchor exhibited a more powerful antiviral effect than recombinant L. plantarum with pgsA. Consistent with this finding, Lb.plantarum-pSIP-409-IFN-λ3-CWA enhanced the expression levels of IFN-stimulated genes (ISGs) (ISG15, OASL, and Mx1) in IPEC-J2 cells more than did recombinant Lb.plantarum-pSIP-409-pgsA'-IFN-λ3. Our study verifies that recombinant L. plantarum inhibits PEDV and TGEV infection in IPEC-J2 cells, which may offer great potential for use as a novel oral antiviral agent in therapeutic applications for combating porcine epidemic diarrhea and transmissible gastroenteritis. This study is the first to show that recombinant L. plantarum suppresses PEDV and TGEV infection of IPEC-J2 cells.

• 한국동물위생학회지
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• 제35권2호
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• pp.83-90
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• 2012

Porcine rotaviruses are the most common causes of viral gastroenteritis in piglets around the world. The major G genotypes of porcine rotaviruses causing diarrhea were G4, G5 and G11 genotypes. Recently, G9 genotype rotaviruses were problemed at swine farms and frequently recognized from diarrheic piglets. In this study, a porcine rotavirus (PoRV-1) was isolated from piglet showing diarrhea using MA104 cells and confirmed as rotavirus by electron microscopy, genomic RNA electropherotyping and indirect immunofluorescence antibody tests. The nucleotide sequence of the VP7 gene of PoRV-1 was determined and compared with those of other genotype rotavirus strains from other parts of the world. Also, the nucleotide sequences of VP4, VP6 and NSP4 genes of PoRV-1 were determined and compared with those of other rotavirus strains from other countries. The results showed that the PoRV-1 isolate belonged to the G9 genotype and the P, I and E genotypes of PoRV-1 were P[23], I5 and E1, respectively. The Korean G9 PoRV-1 isolate and its nucleotide sequence data would be usefully used for the development of porcine rotavirus vaccines in near future.

• Pediatric Infection and Vaccine
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• 제19권1호
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• pp.28-36
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• 2012

목 적 : 이 연구는 경기서부지역의 감염성 설사의 원인 바이러스의 역학 및 지역적 유병율에 대한 정보 제공을 위해 실시되었다. 방 법 : 2009년 1월부터 12월까지 가톨릭대학교 부천성모병원에 급성 설사로 입원한 10세 미만 환아를 대상으로 흔한 세균성 병원체가 없는 310개의 대변 검체에서 rotavirus, parechovirus, adenovirus, astrovirus, enterovirus, norovirus의 유무를 PCR과 RT-PCR을 이용하여 확인하였다. 결 과 : parechovirus (16%)가 가장 흔한 것으로 나타났으며, adenovirus (15%), astrovirus (14%), rotavirus (13%), enterovirus (5%)의 순으로 검출되었다. 단일 감염은 55.8%에서, 중복 감염은 3.2%에서 나타났다. 바이러스성 장염은 전체적으로 두 차례의 유행 시기를 보였으며, 84.6%는 2세 이하에서 발생하였다. 결 론 : parechovirus, adenovirus, astrovirus, enterovirus는 기존에 시행 중인 진단방법으로는 과소평가되어 있으나 소아 설사의 중요한 원인이다.

• 한국동물위생학회지
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• 제31권1호
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• pp.101-111
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• 2008

The purpose of this study was to investigate the protective effects against porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) in suckling piglets by oral administration of IgY. Twenty piglets were divided into two groups with the same number: group I (treated with IgY) and group II (not treated). Group I was administerd orally with IgY for three days from one-day-old and experimentally challenged with PEDV and TGEV at four-day-old. The other was administered with saline solution and challenged with PEDV and TGEV at four-day-old. Serum antibody titers against PEDV and TGEV were examined by enzyme-linked immunosorbent assay (ELISA) and the detection of PEDV or TGEV antigen from feces and small intestines was performed by reverse transcription-polymerase chain reaction (RT-PCR) and indirect immunofluorescence (IFA). The antibody titers of the group I was higher than that of the other, and lasted at the end of experiment. In the detection tests of both virus from feces and small intestine, the rate of the group I was lower. Based on these results, oral administration of IgY may be effective to prevent the diarrhea caused by PEDV and TGEV.