• Title/Summary/Keyword: Garlic shoot

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Systematic Propagation of High Quality Garlic (Allium sativum L.) Through Shoot Apical Meristem Culture 1. Organogenesis from in Vitro Cultured Shoot-tips (생장점배양에 의한 우량마늘 체계적 증식 1.생장점배 양으로부터 기관형성)

  • Lee, Eun-Mo;Lee, Young-Bok
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.3
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    • pp.161-166
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    • 1994
  • Since garlics (Allium sativum L.) are propagated through cloves, infection by virus or other pathogens may become severe problem if not using high quality seed bulbs every year resulting in the reduction of yield and bulb quality, In order to solve this problem, the establishment of virus-free bulb production and its supply system have been required because no chemicals were found to eliminate viruses from seed bulbs. This experiment was conducted to develop an effective production technique of high quality seed bulbs using shoot-tip culture. Over 90% of shoot-tips explanted on January L 1990 were survived at constant temperature of either 20, 24 or 28$^{\circ}C$, wheres 88% at alternate temperature (28/20$^{\circ}C$). The growth of shoot and root was most vigorous at constant 24$^{\circ}C$, and least at alternate temperature (28/20$^{\circ}C$) condition. When shoot-tips were explanted June 21 to August 1,1991, survival and growth of shoot-tips was most vigorous on MS medium supplymented with 0.1 mg/L NAA and 2 mg/L kinetin and least 1 mg/L Gh$_3$. The shoot-tips taken from the seed bulbs stored at 4$^{\circ}C$ for 15 to 60 days were placed on MS medium, shoot growth and in vitro bulblet formation increased slightly as affected by the increase of told treatment period at 4$^{\circ}C$.

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Growth environment and nutritional evaluation of native Allium victorialis var. platyphyllum in Ulrung island (야생 산마늘의 생육 환경과 영양 평가)

  • Choi, Sang-Tai;Lee, Joon-Tak;Park, Woo-Churl
    • Applied Biological Chemistry
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    • v.36 no.6
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    • pp.502-509
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    • 1993
  • In Ulrung floral community the growing environments and nutritive values of wild garlic was investigated. The wild garlic, grown for $3{\sim}4\;years$ in community area, were able to develope bulbs. The bulbs with 2 leaves had the highest ratio of propotion as 75.1% of share among the distribution of bulbs with completely developed leaves and 1 leaf-bulbs and 3 leaves-bulbs had 19.6% and 5.3%, respectively. Also the 2 or 3 leaves-bulbs indicated the better growth states in the part of shoot and bulbs than 1 leaf-bulbs, and there was a significant defferences in each growth between 2 leaves-and 3 leaves-bulbs. On the wild garlics we cannot find out insect injuries. However, there was deseases with symptoms of leaf-spots and lesion from May to June. At first it made a small spots on the leaves, followed by senescence at arrounding area of spots, and then it expanded to whole leaf. This pathogenic bacterium was supposed to be a member of Aristastoma. The contents of crude protein, fat, and carbohydrate in each portion of wild garlics decresed in the order of shoot, expanded leaves and bulbs. On the contrary, crude fibers increased in the same order and so it is not suitable for human consumption nutritional value. However, because the shoots of wild garlics have more than the other Allium sp., the shoots will be a good quality food stuff.

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Assessment of High Temperature Impacts on Early Growth of Garlic Plant (Allium sativum L.) through Monitoring of Photosystem II Activities (광계II 활성 분석을 통한 마늘의 생육초기 고온 스트레스의 영향 평가)

  • Oh, Soonja;Moon, Kyung Hwan;Koh, Seok Chan
    • Horticultural Science & Technology
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    • v.33 no.6
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    • pp.829-838
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    • 2015
  • Garlic (Allium sativum L.), one of the oldest cultivated crops, is the most widely used Allium species belonging to the family Lilliaceae. In this study, growth characteristics, photosystem II activity, and antioxidative enzyme activity were investigated in five temperatures ($10-30^{\circ}C$) during early growth stage of garlic to determine the optimum temperature for cultivation and assess the effects of high temperature on early growth of garlic. Vegetative growth (e.g., shoot height, number of leaves) of garlic plants was greater in the temperature ranges of $15-25^{\circ}C$. However, dry weight (of shoot, bulb, and total plant) of garlic was significantly greater at $20^{\circ}C$, compared to either below or above $20^{\circ}C$. $F_v/F_o$ and $F_v/F_m$ values were highest at $15-20^{\circ}C$, and decreased above $25^{\circ}C$. The chlorophyll a fluorescence induction OKJIP transient was also considerably affected by high temperature; the fluorescence yields $F_i$ and $F_P$ decreased considerably above $25^{\circ}C$, with the increase of $F_k$ and $W_k$. Activities of catalase and superoxide dismutase in leaves and peroxidase in roots were high in $20-25^{\circ}C$, and decreased significantly in $30^{\circ}C$. These results indicate that a growth temperature of $30^{\circ}C$ inhibits early growth of garlic and that it is desirable to culture garlic plants near $20^{\circ}C$. Fluorescence parameters such a $F_v/F_o$, $F_v/F_m$, $F_k$, $ET_o/CS_m$, and $PI_{abs}$ were significantly correlated with dry weight of whole garlic plants (p < 0.01), indicating that these fluorescence parameters can be used for early assessment of high temperature effects even though the damage to the plant is not very severe.

Rapid micropropagation of wild garlic (Allium victorialis var. platyphyllum) by the scooping method

  • Jeong, Mi Jin;Yong, Seong Hyeon;Kim, Do Hyeon;Park, Kwan Been;Kim, Hak Gon;Choi, Pil Son;Choi, Myung Suk
    • Journal of Plant Biotechnology
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    • v.49 no.3
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    • pp.213-221
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    • 2022
  • Wild garlic (Allium victorialis var. platyphyllum, AVVP) is a nontimber forest product used as an edible and medicinal vegetable. AVVP is usually propagated form offspring bulbs but it takes a long time to harvest. Using tissue culture technology could overcome this problem. This study investigated the optimal conditions for shoot multiplication, root growth, and plant growth by scooping AVVP bulbs. AVVP bulbs harvested from Ulleung Island, Korea, the main producer of AVVP, were surface-sterilized and used for in vitro propagation. Shoot multiplication was performed by the scooping method. More than five multiple shoots were induced from scooped tissue in Quoirin and Lepoivre (QL) medium containing plant growth regulators (PGRs); the maximum number of multiple shoots were induced from scooped tissue in QL medium containing 0.45 μM thidiazuron (TDZ) after 16 weeks of culture. Roots were induced directly at the base of the shoots in all treatments. In vitro rooting depended on the type of PGRs, and the best root-inducing treatment was QL medium containing 9.84 μM indole-3-butyric acid (IBA). Plants with in vitro roots were transferred to pots containing artificial soil and successfully acclimatized for 4 weeks. The acclimatized plants showed a survival rate of 80% after 20 weeks and gradually promoted growth depending on the acclimatization period. The results of this study will be of great help to AVVP dissemination through sustainable mass propagation.

Effect of Soaking in KNO3 or K3PO4 Solution and Sowing Date of Bulbils on the Seed Bulbs Production of Garlic (Allium sativum L.) (마늘주아(珠芽)에 대한 KNO3및 K3PO4 처리(處理)와 파종시기(播種時期)가 종구(種球) 생산(生産)에 미치는 영향(影響))

  • Cho, Yong-Cho;Jeong, Byoung-Ryong
    • Horticultural Science & Technology
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    • v.16 no.2
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    • pp.219-221
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    • 1998
  • This study was carried out to investigate seed bulbs production of 'Namdo' garlic (Allium sativum L.) by treating with 200 mM $K_3PO_4$ and 200 mM $KNO_3$ solution to bulbils. The growth characteristics of garlic plants derived from bulbils sown on 3 different dates were compared to those of plants derived from non-treated bulbils. Time required for 50% shoot emergence ($T_{50}$) was shortened as the sowing dates delayed. However, the final emergence rate was increased in the early sowing dates, and was high when treated with $K_3PO_4$ and sown on Oct. 10. The % clove differentiation of bulb was not significantly affected by sowing date and chemicals treatment of the bulbils. In all treatments, % clove differentiation was greater than 85%. The average bulb weight was increased with earlier sowing. Bulbils treated with $K_3PO_4$ and sown on Oct. 10 exhibited the best growth and yield. which was 27% higher compared to the non-treated bulbils.

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Effect of Crude Extracts and Chopped Shoot Application of Allium spp. on Rice Growth (파속 식물의 조추출물과 경엽 처리가 벼의 생육에 미치는 영향)

  • 최상태;안형근;장영득
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.6
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    • pp.625-633
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    • 1996
  • Crude extracts of 4 Allium SPP. such as welsh onion, onion, chinese chives and garlic were purified by paper chromatography and these activities were bio-assayed with rice seedlings. The stem and leaf slices of Allium spp. were treated and rice seedlings were planted in the soil to know the effect of its application on rice growth. The weak acidic fraction of Allium SPP. enhanced the growth and rooting of rice seedlings and had greater activity in promoting than in inhibiting the growth on rice seedling. Elongation of the second leaf sheath of the rice seedlings were not influenced by the extracts of Allium SPP. The stem and leaf application 10 days before transplanting, increased the number of effective tiller remarkably. Especially, the application of 50~400g welsh onion and 50~200g onions increased the number of spikelets per panicle compared to standard fertilization. But, in application of larger amounts, the-death rate of the rice seedlings after transplanting was higher in the stem and leaf application 10 days before transplanting than the one applied on the transplanting day. In particular, treatments of chinese chives and garlic showed higher death rate than those of welsh onion or onion. The stem and leaf application of Allium SPP. resulted in high yield than standard fertilization.

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Photosynthesis and Growth of Southern-type Garlic (Allium sativum L.) in Response to Elevated Temperatures in a Temperature Gradient Tunnel (온도구배터널 내 상승온도에 의한 난지형 마늘(Allium sativum L.)의 광합성 및 생육 특성의 변화)

  • Oh, Seo-Young;Moon, Kyung Hwan;Song, Eun Young;Shin, Minji;Koh, Seok Chan
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.21 no.4
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    • pp.250-260
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    • 2019
  • This study assessed clove germination, shoot growth, photosynthesis and bulb development of southern-type garlic (Allium sativum L.) in a temperature gradient tunnel (TGT), to examine the impacts of increases in temperature on the growth of garlic and find a way to minimize them. The temperatures in the middle and outlet of the TGT were 3.2℃ and 5.8℃ higher, respectively, than the ambient temperature at the tunnel inlet. The germination of garlic cloves was late at temperatures of ambient+3℃ (in the middle of the TGT) and ambient+6℃ (at the outlet) than at ambient temperature (at the inlet). However, bolting and the timing of maximum leaf number per plant were faster at ambient+3℃ or +6℃ than at ambient temperature. Shoot growth was generally greater at ambient temperature. Bulb growth did not significantly differ according to cultivation temperatures, but fresh and dry weights were slightly higher at ambient temperature and ambient+3℃ in the late growth stage. The photosynthesis rate (A), stomatal conductance (gs), and transpiration rate (E) were higher at ambient+3℃ than at ambient temperature. Furthermore, at ambient+3℃, the net photosynthetic rate (Amax) was high, while the dark respiration rate (Rd) was low. At ambient temperature and ambient+3℃, bulb development was healthier, resulting in better productivity and more commercial bulbs, while at ambient+6℃, the bulbs were small and secondary cloves developed, resulting in low commercial value. Therefore, at elevated temperatures caused by global warming, it is necessary to meet the low-temperature requirements before clove sowing, or to delay the sowing time, to improve germination rate and increase yield. The harvest should also be advanced to escape high-temperature stress in the bulb development stage.

A Bibliographical Study on Namul of Koryo and Chosun Dynasty (고려와 조선시대의 문헌으로 본 한국의 나물)

  • 강은주
    • The Korean Journal of Food And Nutrition
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    • v.6 no.1
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    • pp.16-24
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    • 1993
  • The "Namul" is a Korean common vegetable food composed of edible young leaves and soft stalks of wild plants or cultivating vegetables and treated with traditional condiments. It has been widely used as not only a regular diet but seasonal foods or special ceremonial foods. In this thesis, the kinds of edible plants for namul were philologically approached by books published in Korea from Koryo to Chosun dynasty. The first historical record about cultivated vegetable as food in Korea was a garlic and a gourd in $\ulcorner$Samkuksaki$\lrcorner$. According to numerous records, edible wild plants might have played an important part as food resources, since they have variable edible portions as famine relief foods. Four kinds of namul were first introduced in Koryo age, but in Chosun dynasty over eighty kinds were recorded. The root of ballonflower and white radish were most commonly used for raw namul and bamboo shoot and squash for boils ones. Most kinds of namul were introduced in $\ulcorner$Zeungtosanrimkwungiae$\lrcorner$ and $\ulcorner$Limwonsiprwukji$\lrcorner$ influenced by pragmatism in later half period of Chosun. The chronic state of famine in later half period of Chosun caused to introduce various edible wild vegetables, and had greatly influenced on the Korean people to acquire vegetarian food habits and to favor salty taste. It seems to need a further study on recent nutritional problems including dietary fiber.ary fiber.

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