• Proceedings of the Korean Nuclear Society Conference
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• 1999.10a
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• pp.194-194
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• 1999

• Journal of the korean academy of Pediatric Dentistry
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• v.26 no.2
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• pp.399-415
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• 1999

From bacteria to mammalian cells, one of the most important mediators of intracellular signal transduction mechanisms which regulate a variety of intracellular processes is free calcium. In salivary acinar cells, elevation of intracellular calcium concentration ($[Ca^{2+}]_i$) is essential for the salivary secretion induced by parasympathetic stimulation. However, in addition to $[Ca^{2+}]_i$, gap junctions which couple individual cells electrically and chemically have also been reported to regulate enzyme secretion in pancreatic acinar cells. Since the plasma membrane of salivary acinar cells has a high density of gap junctions, and these cells are electrically and chemically coupled with each other, gap junctions may modulate the secretory function of salivary glands. In this respect, I planned to investigate the role of gap junctions in the modulation of salivary secretion and $[Ca^{2+}]_i$, using mandibular salivary glands of rats. In order to measure the salivary flow rate, fluid was collected from the cannulated duct of the isolated perfused rat mandibular glands at 2 min intervals. $[Ca^{2+}]_i$, was measured from the cells loaded with fura-2 by spectrofluorometry. The results obtained were as follows: 1. CCh-induced salivary secretion was reversibly inhibited by 1 mM octanol, a gap junction blocker. 2. CCh-induced increase in $[Ca^{2+}]_i$, was also reversed by the application of 1 mM octanol. 3. Octanol did not block the initial increase in $[Ca^{2+}]_i$ caused by CCh, which suggested that the reduction of $[Ca^{2+}]_i$, caused by gap junction blockade was not resulted from the inhibition of $Ca^{2+}$ release from intracellular $Ca^{2+}$ stores. 4. Addition of octanol during stimulation with $1{\mu}M$ thapsigargin, a potent microsomal ATPase inhibitor, reduced $[Ca^{2+}]_i$, to the basal level. This suggested that inhibition of gap junction permeability closed plasma membrane $Ca^{2+}$ channels. 5. 2,5-di-tert-butyl-1,4-benzohydroquinone (TBQ) generated $[Ca^{2+}]_i$ oscillations resulting from periodic influx of $Ca^{2+}$ via plasma membrane. The TBQ-induced $[Ca^{2+}]_i$ oscillations were stopped by the application of 1mM octanol which implicated that gap junctions modulate the permeability of plasma membrane $Ca^{2+}$ channels. 6. Glycyrrhetinic acid, another well known gap junction blocker, also inhibited CCh-induced salivary secretion from rat mandibular glands. These results suggested that gap junctions play an important role in the modulation of fluid secretion from the rat mandibular glands and this was probably due to the inhibition of $Ca^{2+}$ influx through the plasma membrane $Ca^{2+}$ channels.

• Nuclear Engineering and Technology
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• v.19 no.1
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• pp.22-33
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• 1987

The confirmed integrity of nuclear fuel cladding materials is an important object during steady state and transient operations at nuclear power plant. In this context, the clad material yielding behavior is especially important because of pellet-clad gap expansion. During the steep power excursion, the in-pile irradiation behavior differences between uranium-dioxide fuel pellet and zircaloy clad induce the contact pressure between them. If this pressure reaches the zircaloy clad yield pressure, the zircaloy clad will be plastically deformed. After the reactor power resumed to normal state, this plastic permanent expansion of clad tube give rise to the pellet-clad gap expansion. In this paper, the simple mandrel expansion test method which utilizes thermal expansion difference between copper mandrel and zircaloy tube was adopted to simulate this phenomenon. That is, copper mandrel which has approximately three times of thermal expansion coefficient of zircaloy-4 (PWR fuel cladding material) were used in this experiment at the temperature range from 400C to 700C. The measured plastic expansion of zircaloy outer radius and derived mathematical relations give the yield pressure, yield stress of zircaloy-4 clad at the various clad wall temperatures, the activation energy of zircaloy tube yielding, and pellet-clad gap expansion. The obtained results are in good agreement with previous experimental results. The mathematical analysis and simple test method prove to be a reliable and simple technique to assess the yielding behavior and gap expansion measurement between zircaloy-4 tube and uranium-dioxide fuel pellet under biaxial stress conditions.

• Korean Journal of Food Science and Technology
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• v.39 no.5
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• pp.558-563
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• 2007

This study was conducted to analyze the cytotoxic effects of butylated hydroxytoluene (BHT) and propyl gallate (PG) in WB-F344 rat liver epithelial cells. Here we measured the inhibition level of gap junctional intercellular communication (GJIC) and elucidated the relationships between GJIC and mitogen-activated protein kinases (MAPKs) such as ERK, JNK, and p38. The cytotoxicities of BHT and PG appeared at concentrations of 1.0mM and 0.25mM, respectively, in the WB-F344 cells; and GJIC inhibition, which was analyzed by a scrape-loading/dye transfer assay and Western blotting analysis, appeared at 0.6mM for BHT and 0.1mM for PG, respectively. Also, the phosphorylations of Cx43, ERK, JNK, and p38 increased in dose-dependent manners. This suggests that BHT and PG treatments inhibited GJIC by the phosphorylation of MAPKs prior to cell damage.

• Survey Research
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• v.10 no.2
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• pp.1-21
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• 2009

The purpose of this study is to compare the estimates of housework time by gender using two representative methods of time use study: Time Diary and Stylized Survey Questionnaire. Our analysis is based on the data from the Lifetime Use Survey(2004), which used time-diary questions, and the Korean Labor & Income Panel Study(KLIPS 2004), which used stylized questions on housework hours. The results show that men over-report their housework time in the stylized time use questions. In contrast, women under-report their housework time, which is unusual in the previous studies on response errors and reporting gap. Subgroup analysis shows that widowed/divorced men tend to over-report their contribution to housework more than other groups whereas among women, groups burdened with employed work, caring and housework underestimate their housework time. This reporting gap is explained by gendered norm and perception of time pressure. The theory to explain under-reporting of the housework time has been undeveloped in the previous studies. Our study suggests that perceptions of time pressure be an important factor to explain women's reporting gap of housework estimates.

• Journal of the Korean Society of Propulsion Engineers
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• v.8 no.2
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• pp.46-53
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• 2004

Ultrasonic testing method has been developed to evaluate flaw of adhesive layers in blast tube for the reliability of the rocket nozzle. The ultrasonic reflection from the interface between the steel sheet and the epoxy adhesive is measured with a high-frequency Pulse-echo setup in order to identify contact debonding and missing adhesive in epoxy layer between steel and FRP layers. The steel sheet is resonated by low-frequency ultrasound, and the gap size underneath the measuring location is estimated from the resonance responses. For practical application in industry an automated testing system has been developed where the proposed approach is implemented. The performance of the proposed approach has been verified by actual measurement of gap sizes from the cross-sections of cut specimens using an optical microscope.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2003.10a
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• pp.230-237
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• 2003

Ultrasonic testing method has been developed to evaluate adhesive layers in blast tube for the reliability of the rocket. The main objective of the present work was to find debonding and missing adhesive in epoxy layer between steel and FRP layers. In this approach, the ultrasonic reflection from the interface between the steel sheet and the epoxy adhesive is measured with a high-frequency pulse-echo setup in order to identify contact debonding and missing adhesive. Then, the steel sheet is excited to resonance by low-frequency ultrasound, and the gap size underneath the measuring location is estimated from the resonance responses. For practical application in industry an automated testing system has been developed where the proposed approach is implemented. The performance of the proposed approach has been verified by actual measurement of gap sizes from the cross-sections of cut specimens using an optical microscope.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.14 no.12
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• pp.1269-1275
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• 2003

In this paper, nonradiative dielectric(NRD) rotman lens with a gap-coupled unidirectional dielectric radiator(UDR) has been designed. Gap-coupled UDR is structurally suitable for NRD rotman lens. We have optimized NRD rotman lens for minimizing side-lobe, and calculated design parameters of UDR such as length of resonator and distance of gap using an equivalent circuit model of an evanescent NRD guide. Experimental prototype of UDR is fabricated and measured at the center frequency of 38 GHz. The simulated S-parameter and far-field radiation beam pattern of UDR show good agreements with measured data. Finally, total beam pattern of NRD rotman lens of multi-beam feed has been obtained using a measured pattern of UDR and array factor of NRD rotman lens. The obtained beam pattern shows remarkably suppressed side-lobe.

• Journal of the Society of Naval Architects of Korea
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• v.43 no.4 s.148
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• pp.422-430
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• 2006

The horn and movable parts around the gap of the conventional semi-spade rudder are visualized by high speed CCD camera with the frame rate of 4000 fps (frame per second) to study the unsteady cavity pattern on the rudder surface and gap. In addition, the pressure measurements are conducted on the rudder surface and inside the gap to find out the characteristics of the flow behavior. The rudder without propeller wake is tested at the range of $1.0{\leq}{\sigma}_v\;1.6$ and at the rudder deflection angle of $-8{\leq}{\theta}{\leq}10^{\circ}$. The time resolved cavity images are captured and show strong cavitation around the rudder gap in all deflection angles. As the deflection angle gets larger, the flow separated from the horn surface increases the strength of cavitation. The accelerated flow along the horn decreases its pressure and the separated flow from the horn increases the pressure abruptly. The pressure distribution inside the gap reveals the flow moving from the pressure to suction side. In the negative deflection angle, the turning area on the movable part initiates the flow separation and cavitation on it.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.11 no.6
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• pp.990-995
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• 2000

As the interest in the millimeter wave frequency (30 ~300 GHz) increases, Nonradiative Dielectric (NRD) guide is being more attractive due to its low loss characteristics. Most of millimeter wave components, which can be realized with waveguide, can also be realized with NRD guide since NRD guide has similar dispersion characteristics and field patterns to waveguide. Previously, Variational Method was applied to the gap discontinuity problem to design a gap-coupled NRD bandpass filter. In this paper, the design procedure was simplified by replacing the air gap region with an equivalent circuit model of an evanescent waveguide using the fact that the NRD guide has a similar structure with a dielectric-filled metal waveguide. Prior to applying this design method to the bandpass filter of millimeter wave frequency range, a bandpass filter of which center frequency is 10 GHz(3-Pole, 0.1 dB ripple, 2% fractional bandwidth) was designed and fabricated. The measured result agrees with one simulated with HFSS within an error range of a fabrication.