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Inhibition of Gap Junctional Intercellular Communication in Rat Liver Epithelial Cells Induced by BHT and Propyl Gallate  

Kim, Ji-Sun (Korea Food Research Institute)
Kim, Sung-Ran (Korea Food Research Institute)
Ahn, Ji-Yun (Korea Food Research Institute)
Ha, Tae-Youl (Korea Food Research Institute)
Kang, Kyoung-Sun (Department of Veterinary Public Health, Seoul National University)
Kim, Sun-A (Korea Food Research Institute)
Publication Information
Korean Journal of Food Science and Technology / v.39, no.5, 2007 , pp. 558-563 More about this Journal
Abstract
This study was conducted to analyze the cytotoxic effects of butylated hydroxytoluene (BHT) and propyl gallate (PG) in WB-F344 rat liver epithelial cells. Here we measured the inhibition level of gap junctional intercellular communication (GJIC) and elucidated the relationships between GJIC and mitogen-activated protein kinases (MAPKs) such as ERK, JNK, and p38. The cytotoxicities of BHT and PG appeared at concentrations of 1.0mM and 0.25mM, respectively, in the WB-F344 cells; and GJIC inhibition, which was analyzed by a scrape-loading/dye transfer assay and Western blotting analysis, appeared at 0.6mM for BHT and 0.1mM for PG, respectively. Also, the phosphorylations of Cx43, ERK, JNK, and p38 increased in dose-dependent manners. This suggests that BHT and PG treatments inhibited GJIC by the phosphorylation of MAPKs prior to cell damage.
Keywords
gap junctional intercellular communication; butylated hydroxy toluene; propyl gallate; MAPKs;
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Times Cited By KSCI : 1  (Citation Analysis)
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