• Title/Summary/Keyword: Gap 측정

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Construction of an Expression Vector System with the GAP Promoter in Saccharomyces cerevisiae (효모, Saccharomyces cervisiae의 GAP 유전자를 이용한 발현 벡터계의 개발)

  • 황요일;서애란;심상국;정동효
    • Microbiology and Biotechnology Letters
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    • v.19 no.6
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    • pp.568-574
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    • 1991
  • The cloned glyceraldehyde-3-phosphate dehydrogenase (GAP) gene of Saccharomyces cereviszae (Holland et al., 1983) has been characterized. Based on the communication, we have also cloned 2.1 kb CAP DNA fragment and modified this fragment as a portable promoter. Two yeast expression vectors, one is YCp type vector being maintained at low copy number (1 or 2) and the other is YEp type vector at high copy number, have been constructed with the GAP promoter and the PH05' gene as a reporter. Our plasrnids were introduc,ed into S. cerevisiae HY-1, which has been improved. The $Trp^+$ transformants expressed APase activity efficiently and showed high level of PH05' transcripts.

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MEMS Capacitive Gap Sensor for Measuring Abrasion Depth of Gun Barrel Rifling (포신 강선의 마모 깊이 측정을 위한 정전용량 방식의 MEMS 간극센서)

  • Lee, Seok-Chan;Lee, Seung-Seob;Lee, Chang-Hwa
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.33 no.9
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    • pp.976-981
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    • 2009
  • MEMS capacitive gap sensor is developed for measuring abrasion depth of gun barrel rifling. Measuring abrasion depth of gun barrel rifling is very important because it is related with exactness of firing and life of arms. The method using a gap sensor is not to hurt rifling. And it can measure abrasion depth through minimum shooting, because the developed gap sensor can measure from $1{\mu}m{\sim}12{\mu}m$ using Polydimethylsiloxane(PDMS) material and making a stretchable electrode on PDMS. And it's resolution is 1 ${\mu}m$ using capacitive method and MEMS technology.

Effects of Aging Process in Kinetic Movement of Charged Paticles (에이징 공정이 대전입자 운동에 미치는 영향)

  • Kim, In-Ho;Kim, Young-Cho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.6
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    • pp.1175-1179
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    • 2009
  • We analyze voltage characteristics of charged particle type display according to particle layers and cell gap between two electrodes and ascertain the aging effects by measuring the response time of particles with and without aging process. The threshold/driving/breakdown voltage is proportional to layers of charged particles and cell gap and the response time at driving voltage is faster than that of threshold and breakdown voltage because of different q/m of color and black particles. The analysis of response time is a method of estimation of optical characteristics, driving voltage and particle lumping and these results are promoted by aging process. We use the laser and photodiode to measure response time and optical properties. It has not been studied and reported to analyze the relationship of response time, threshold/driving/breakdown voltage, lumping phenomena, cell gap, and aging process for charged particle type display.

Evaluation of Dosimetric Leaf Gap (DLG) at Different Depths for Dynamic IMRT (동적 세기조절방사선치료에서 깊이에 따른 DLG변화 분석)

  • Chang, Kyung Hwan;Kwak, Jungwon;Cho, Byungchul;Jeong, Chiyoung;Bae, Jae Beom;Yoon, Sang Min;Lee, Sang-wook
    • Progress in Medical Physics
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    • v.26 no.3
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    • pp.153-159
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    • 2015
  • This study is to evaluate thedosiemtric leaf gap (DLG) at different depths for dynamic intensity-modulated radiation therapy (IMRT) in order to evaluate the absolute dose and dose distribution according to the different positions of tumors and compare the measured and planned the multileaf collimator (MLC) transmission factor (T.F.) and DLG values. We used the 6 MV and 15 MV photon beam from linear accelerator with a Millenium 120 MLC system. After the import the DICOM RT files, we measured the absolute dose at different depths (2 cm, 5 cm, 10 cm, and 15 cm) to calculate the MLC T. F. and DLG. For 6 MV photon beam, the measured both MLC T. F. and DLG were increased with the increase the measured depths. When applying to treatment planning systemas fixed transmission factor with its value measured under the reference condition at depth of 5 cm, although the difference fixed and varied transmission factor is not significant, the dosiemtric effect could be presented according to the depth that the tumor is placed. Therefore, we are planning to investigate the treatment planning system whichthe T. F. and DLG factor according to at the different depths can be applied in the patient-specific treatment plan.

Development of Ultrasonic Magnetostrictive Sensors System to Measure in Very High Temperatures (초고온 온도 측정을 위한 초음파 자왜 센서 시스템 개발)

  • 구길모;김상백;박치승;최종호;고덕영
    • The Journal of the Acoustical Society of Korea
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    • v.20 no.6
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    • pp.66-74
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    • 2001
  • The temperature measurement of yen high temperature core melt is of importance in LAVA (Lower-plenum Arrested Vessel Attack) experiment in which gap formation between core melt and the reactor lower head, and the effect of the gap on thermal behavior are to be measured. The delay time of ultrasonic wavelets due to high temperature is suggested. As a first stage, a molten material temperature was measured up to 2300℃. Also, the optimization design of the ultrasonic temperature sensor with persistence at the high temperature was suggested in this paper. And the utilization of the theory suggested in the reference〔1〕and the efficiency of the developed system are certified by performing experiments. This sensor welded magnetostrictive element and tungsten element will be able to measure a temperature range of 3000℃ hereafter.

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Estimation of Discharge Current Waveshapes in Short Gap Discharge by Radiated Electromagnetic Fields (방사 전자파에 의한 미소 간극 방전원의 방전 전류파형 추정)

  • 김기채;이광식;이동인
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.11 no.2
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    • pp.259-267
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    • 2000
  • This paper presents the method for an estimation of discharge current waveshapes in short gap discharge by radiated electromagnetic fields. The method of current waveform estimation described is using the one antenna method(single field method) and two antennal method(complex field method) with a measured electric or magnetic fields at given field point by a time domain antenna. In order to verify the availability of the estimation theory, the discharge current waveshape estimation was performed by one and two antenna methods using the measured electric fields of Wilson & Ma and compared with experiments.

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Optical characterization of doped ZnO thin films

  • Kim, Jin-Su;Jo, Seong-Hun;Seong, Tae-Yeon;Kim, Won-Mok
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.06a
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    • pp.426-426
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    • 2008
  • ZnO 박막과 Al이 도핑된 ZnO 다결정질 박막을 rf magnetron sputtering 방법을 이용하여 Si(100) 기판과 코닝글라스 기판에 증착하여 박막의 광학적 특성을 Spectro-scopic Ellipsometry (SE, Woollam사)와 UV-VIR-NIR Sphectrophotometry (SP, Varian사)를 사용하여 분석하였다. SE 측정은 입사각도 55도에서 75도까지 5도 간격으로 파장범위 250 - 1700 nm 에서 3 nm 간격으로 측정하였으며, SP 측정은 수직입사로 250-3000 nm 파장범위에서 1 nm 간격으로 투과도와 반사도를 측정하였다. 측정된 데이터들은 Lorentz Oscillator 모델과 Drude free electron 모델이 결합된 분산관계식을 사용하여 전산 맞춤을 하여 분석하였다. ZnO 박막의 optical band gap energy 는 3.3 eV로 측정되었으며, Al 도핑에 따른 자유전하농도가 증가에 의하여 Burstein-Moss 효과에 따르는 optical band gap energy의 증가 거동을 보였다. 또한 자유전하농도 증가에 따라 band edge 부근에서 나타나는 excitonic transition 에 기인하는 유전함수 피크의 broadening이 관찰되었으며, high frequency dielectric constant는 자유 전하농도에 관계없이 3.689${\pm}$0.05 eV 의 값을 가졌다. Drude free electron 모델을 사용하여 plasma frequency를 구하고 이로부터 얻어진 optical mobility 와 Hall mobility를 비교하여 ZnO계 다결정질 박막에서의 결정립계가 이동도에 미치는 영향을 고찰하고자 한다.

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A Comparative Study of a Variable Overlap-area Type and Conventional Types in the Inductive Precision Position Measurement System (자속경로 단면적 및 공극변화를 이용한 인던턴스형 초정밀 변위측정 시스템)

  • Choe, Dong-Jun;Choe, In-Muk;Kim, Su-Hyeon
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.26 no.2
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    • pp.254-259
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    • 2002
  • A variable air-gap type system is widely used for inductive precision position measurement systems. This type transducer has high sensitivity but lacks a linear measurement range due to structural nonlinearity. Furthermore, as measurement range increases, linearity error is also increased. The alternative is a variable overlap-area type system. The sensitivity of this type is determined by the initial air-gap dimension, keeps the original value and does not deteriorate linearity in spite of the variations of the measuring range.

A Study on the Selection of Measuring Mode in the Permittivity Measurement Using a Circular Cylindrical Cavity (원통형 공진기를 이용한 유전율 측정방법에서 측정모드 선택에 관한 연구)

  • Lee, Won-Hui;Kang, Soon-Kuk;Choi, Hong-Ju;Hur, Jung;Lee, Sang-Young
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.10 no.2
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    • pp.218-226
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    • 1999
  • This paper describes resonant mode selection with which the relative permittivity can be measured exactly. To measure the relative permittivity, a circular cylindrical cavity filled with dielectric material is used. When the circular cylindrical cavity is filled with the dielectric material, the air gap occurs on account of machining error. Accurate relative permittivity can be obtained by using less sensitive mode in resonant frequency variation by the air gap. As a result, Average 0.009% resonant frequency variation in the vertical and the radial direction appears at $TE_{011}$ mode. It is interesting that the frequency variation by the air gap at $TE_{011}$ mode turns out to be the least sensitive.

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A Study on the Correlation of MESG and Explosion Pressure (최대실험안전틈새(MESG)와 폭발압력의 상관관계에 대한 연구)

  • Hwang, Kyungyong;Shin, Woonchul;Lee, Taeck-Kie
    • Journal of the Korean Institute of Gas
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    • v.20 no.1
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    • pp.29-39
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    • 2016
  • Electrical apparatuses for use in the presence of explosive gas atmospheres have to be special designed to prevent them from igniting the explosive gas. Flameproof design implies that electrical components producing electrical sparks are contained in enclosures and withstand the maximum pressure of internal gas or vapours. In addition, any gaps in the enclosure wall have to designed in such a way that they will not transmit a gas explosion inside the enclosure to an explosive gas or vapours atmosphere outside it. In this study, we explained some of the most important physical mechanism of Maximum Experimental Safe Gap(MESG) that the jet of combustion products ejected through the flame gap to the external surroundings do not have an energy and temperature large enough to initiate an ignition of external gas or vapours. We measured the MESG and maximum explosion pressure of propane and acetylene by the test method and procedure of IEC 60079-20-1:2010.When the minimum MESG is measured, the concentration of propane, acetylene in the air is higher than the stoichiometric point and their explosion pressure is the highest value.