• Parasites, Hosts and Diseases
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• v.59 no.2
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• pp.167-171
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• 2021

Haemonchosis remains a significant problem in small ruminants. In this study, the assay of recombinase polymerase amplification (RPA) combined with the lateral flow strip (LFS-RPA) was established for the rapid detection of Haemonchus contortus in goat feces. The assay used primers and a probe targeting a specific sequence in the ITS-2 gene. We compared the performance of the LFS-RPA assay to a PCR assay. The LFS-RPA had a detection limit of 10 fg DNA, which was 10 times less compared to the lowest detection limit obtained by PCR. Out of 24 goat fecal samples, LFS-RPA assay detected H. contortus DNA with 95.8% sensitivity, compared to PCR, 79.1% sensitivity. LFS-RPA assay did not detect DNA from other related helminth species and demonstrated an adequate tolerance to inhibitors present in the goat feces. Taken together, our results suggest that LFS-RPA assay had a high diagnostic accuracy for the rapid detection of H. contortus and merits further evaluation.

• Proceedings of the Korea Water Resources Association Conference
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• 2001.05a
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• pp.292-297
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• 2001

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.4
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• pp.1517-1520
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• 2014

Aim: To investigate effects of sulforaphane on the BIU87 cell line and underlying mechanisms involving IGFBP-3. Methods: Both BIU87 and IGFBP-3-silenced BIU87 cells were treated with sulforaphane. Cell proliferation was detected by MTT assay. Cell cycle and apoptosis were determined via flow cytometry. Quantitative polymerase chain reaction and Western blotting were applied to analyze the expression of IGFBP-3 and NF-${\kappa}B$ at both mRNA and protein levels. Results: Sulforaphane (80 ${\mu}M$) treatment could inhibit cell proliferation, inducing apoptosis and cell cycle arrest at G2/M phase. All these effects could be antagonized by IGFBP-3 silencing. Furthermore, sulforaphane (80 ${\mu}M$) could down-regulate NF-${\kappa}B$ expression while elevating that of IGFBP-3. Conclusions: Sulforaphane could suppress the proliferation of BIU87 cells via enhancing IGFBP-3 expression, which negatively regulating the NF-${\kappa}B$ signaling pathway.

• Parasites, Hosts and Diseases
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• v.58 no.6
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• pp.681-687
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• 2020

Giardia lamblia is a common enteric pathogen associated with diarrheal diseases. There are some reports of G. lamblia infection among different breeds of cattle in recent years worldwide. However, it is yet to know whether cattle in Jiangxi province, southeastern China is infected with G. lamblia. The objectives of the present study were to investigate the prevalence and examine the multilocus genotypes of G. lamblia in cattle in Jiangxi province. A total of 556 fecal samples were collected from 3 cattle breeds (dairy cattle, beef cattle, and buffalo) in Jiangxi province, and the prevalence and genotypes of G. lamblia were determined by the nested PCR amplification of the beta-giardin (bg) gene. A total of 52 samples (9.2%) were positive for G. lamblia. The highest prevalence of G. lamblia was detected in dairy cattle (20.0%), followed by that in beef cattle (6.4%), and meat buffalo (0.9%). Multilocus sequence typing of G. lamblia was performed based on sequences of the bg, triose phosphate isomerase and glutamate dehydrogenase loci, and 22, 42, and 52 samples were amplifiable, respectively, forming 15 MLGs. Moreover, one mixed G. lamblia infection (assemblages A and E) was found in the present study. Altogether, 6 novel assemblage E subtypes (E41*-E46*) were identified for the first time. These results not only provided baseline data for the control of G. lamblia infection in cattle in this southeastern province of China, but also enriched the molecular epidemiological data and genetic diversity of G. lamblia in cattle.

• Earthquakes and Structures
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• v.15 no.2
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• pp.133-144
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• 2018

To study the effectiveness of sliding isolation in a CRLSS (concrete rectangular liquid-storage structure) and develop a reasonable limiting-device method, dynamic responses of non-isolation, sliding isolation with spring limiting-devices and sliding isolation with steel bar limiting-devices are comparatively studied by shaking table test. The seismic response reduction advantage of sliding isolation for concrete liquid-storage structures is discussed, and the effect of the limiting-device type on system dynamic responses is analyzed. The results show that the dynamic responses of sliding isolation CRLSS with steel bar-limiting devices are significantly smaller than that of sliding isolation CRLSS with spring-limiting devices. The structure acceleration and liquid sloshing wave height are greatly influenced by spring-limiting devices. The acceleration of the structure in this case is close to or greater than that of a non-isolated structure. Liquid sloshing shows stronger nonlinear characteristics. On the other hand, sliding isolation with steel bar-limiting devices has a good control effect on the structural dynamic response and the liquid sloshing height simultaneously. Thus, a limiting device is an important factor affecting the seismic response reduction effect of sliding isolation. To take full advantage of sliding isolation in a concrete liquid-storage structure, a reasonable design of the limiting device is particularly important.

• Earthquakes and Structures
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• v.18 no.4
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• pp.467-480
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• 2020

Concrete rectangular liquid storage tanks are widely used, but there are many cases of damage in previous earthquakes. Nonlinear fluid-structure interaction (FSI) is considered, Mooney-Rivlin material is used for rubber bearing, nonlinear contact is used for sliding bearing, numerical calculation models of no-isolation, rubber isolation, sliding isolation and hybrid isolation concrete rectangular liquid storage tanks are established; dynamic responses of different structures are compared to verify the effectiveness of isolation methods; and influences of earthquake amplitude, bidirectional earthquake and far-field long-period earthquake on dynamic responses are investigated. Results show that for liquid sloshing wave height, rubber isolation cause amplification effect, while sliding isolation and hybrid isolation have reduction effect; displacement of rubber isolation structure is much larger than that of sliding isolation with limiting-devices and hybrid isolation structure; when PGA is larger, wall cracking probability of no-isolation structure becomes larger, and probability of liquid sloshing wave height and structure displacement of rubber isolation structure exceeds the limit is also larger; under bidirectional earthquake, occurrence probabilities that liquid sloshing wave height and structure displacement of rubber isolation structure exceed the limit will be increased; besides, far-field long-period earthquake mainly influences structure displacement and liquid sloshing wave height. On the whole, control effect of sliding isolation is the best, followed by hybrid isolation, and rubber isolation is the worst.

• Geomechanics and Engineering
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• v.20 no.2
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• pp.155-164
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• 2020

In recent years, the stability, safety and comfort of trains has received increased attention. The mechanical characteristics and differential settlement of the foundation are the main problems studied in high-speed railway research. The mechanical characteristics and differential settlement of the foundation are greatly affected by the ground treatment. Additionally, the effects of train load and earthquakes have a great impact. The dynamic action of the train will increase the vibration acceleration of the foundation and increase the cumulative deformation, and the earthquake action will affect the stability of the substructure. Earthquakes have an important practical significance for the dynamic analysis of the railway operation stage; therefore, considering the impact of earthquakes on the railway substructure stability has engineering significance. In this paper, finite element model of the CFG (Cement Fly-ash Gravel) pile + cement-soil compacted pile about composite foundation is established, and manual numerical incentive method is selected as the simulation principle. The mechanical characteristics and differential settlement of CFG pile + cement-soil compacted pile about composite foundation under train load are studied. The results show: under the train load, the neutral point of the side friction about CFG pile is located at nearly 7/8 of the pile length; the vertical dynamic stress-time history curves of the cement-soil compacted pile, CFG pile and soil between piles are all regular serrated shape, the vertical dynamic stress of CFG pile changes greatly, but the vertical dynamic stress of cement-soil compacted pile and soil between piles does not change much; the vertical displacement of CFG pile, cement-soil compacted pile and soil between piles change very little.

• Structural Engineering and Mechanics
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• v.66 no.3
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• pp.411-421
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• 2018

Although a rubber isolation cushion can reduce the dynamic response of a structure itself, it has little influence on the height of a sloshing wave and even may induce magnification action. Vertical baffles are set into a base-isolated Concrete Rectangular Liquid Storage Structure (CRLSS), and baffles are opened as holes to increase the energy dissipation of the damping. Problems of liquid nonlinear motion caused by baffles are described using the Navier-Stokes equation, and the space model of CRLSS is established considering the Fluid-Solid Interaction (FSI) based on the Finite Element Method (FEM). The dynamic response of an isolated CRLSS with various baffles under an earthquake is analyzed, and the results are compared. The results show that when the baffle number is certain, the greater the number of holes in baffles, the worse the damping effects; when a single baffle with holes is set in juxtaposition and double baffles with holes are formed, although some of the dynamic response will slightly increase, the wallboard strain and the height of the sloshing wave evidently decrease. A configuration with fewer holes in the baffles and a greater number of baffles is more helpful to prevent the occurrence of two failure modes: wallboard leakage and excessive sloshing height.

• Parasites, Hosts and Diseases
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• v.61 no.1
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• pp.42-52
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• 2023

The genus Babesia includes parasites that can induce human and animal babesiosis, which are common in tropical and subtropical regions of the world. The gut microbiota has not been examined in hamsters infected by Babesia duncani. Red blood cells infected with B. duncani were injected into hamsters through intraperitoneal route. To evaluate the changes in gut microbiota, DNAs were extracted from small intestinal contents, acquired from hamsters during disease development. Then, the V4 region of the 16S rRNA gene of bacteria was sequenced using the Illumina sequencing platform. Gut microbiota alternation and composition were assessed according to the sequencing data, which were clustered with >97.0% sequence similarity to create amplicon sequence variants (ASVs). Bacteroidetes and Firmicutes were made up of the major components of the gut microbiota in all samples. The abundance of Bacteroidetes elevated after B. duncani infection than the B. duncani-free group, while Firmicutes and Desulfobacterota declined. Alpha diversity analysis demonstrated that the shown ASVs were substantially decreased in the highest parasitemia group than B. duncani-free and lower parasitemia groups. Potential biomarkers were discovered by Linear discriminant analysis Effect Size (LEfSe) analysis, which demonstrated that several bacterial families (including Muribaculaceae, Desulfovibrionaceae, Oscillospiraceae, Helicobacteraceae, Clostridia UGG014, Desulfovibrionaceae, and Lachnospiraceae) were potential biomarkers in B. duncani-infected hamsters. This research demonstrated that B. duncani infectious can modify the gut microbiota of hamsters.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.10
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• pp.5325-5325
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• 2012