• IMMUNE NETWORK
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• v.3 no.2
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• pp.136-144
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• 2003

Oral administration of antigen has long been considered as a promising alternative for the treatment of chronic autoimmune diseases including rheumatoid arthritis (RA), and oral application of type II collagen (CII) has been proven to improve pathogenic symptoms in RA patients without problematic side effects. To further current understandings about the immune suppression mechanisms mediated by orally administered antigens, we examined the changes in IgG subtypes, T-cell proliferative response, and proportion of interleukin (IL)-10 producing Th subsets in a time course study of collagen induced arthritis (CIA) animal models. We found that joint inflammation in CIA mouse peaked at 5 weeks after first immunization with CII, which was significantly subdued in mice pre-treated by repeated oral administration of CII. Orally tolerized mice also showed increase in their serum level of IgG1, while the level of IgG2a was decreased. T-cell proliferation upon CII stimulation was also suppressed in lymph nodes of mice given oral administration of CII compared to non-tolerized controls. When cultured in vitro in the presence of CII, T-cells isolated from orally tolerized mice presented higher proportion of $CD4^+IL-10^+$ subsets compared to non-tolerized controls. Interestingly, such increase in IL-10 producing cells were obvious first in Peyer's patch, then by 5 weeks after immunization, in mesenteric lymph node and spleen instead. This result indicates that a particular subset of T-cells with immune suppressive functions might have migrated from the original contact site with CII to inflamed joints via peripheral blood after 5 weeks post immunization.

• The Korean Journal of Nuclear Medicine
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• v.32 no.1
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• pp.89-98
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• 1998

The search for tumor-avid agents for use in nuclear medicine imaging is an ongoing field of importance. The purpose of this study was to determine the affinity for radio labeled vitamin $B_{12}$ by a wide histologic variety of tumor types in mice. Seventeen different types of tumor were grown subcutaneously in female Balb/C or Balb nu/nu(nude) mice. When the tumors reached about 1 cm in diameter, mice were injected intraperitoneally with $^{57}Co$-vitamin $B_{12}$. Twenty-four hours later, the mice were sacrificed. Organs and tissues were removed, weighed, and activity per mg determined by gamma counter. Values represented cpm/mg tissue that was normalized to 20 grams body weight for each mouse. A wide variety of tumor types showed significant uptake and concentration of $^{57}Co$-vitamin $B_{12}$, as evidenced by tumor:tissue activity ratios. For many tissues of great importance in terms of background(bone, muscle, blood), the tumor:tissue activity ratios of uptake were high. These data strongly suggest that further efforts to evaluate the utility of radio labeled adducts of vitamin $B_{12}$ for clinical use in oncologic imaging are warranted.

• Journal of Physiology & Pathology in Korean Medicine
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• v.26 no.5
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• pp.724-731
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• 2012

This study was designed to elucidate whether combination with Korean red ginseng and Morus alba L. (MPM), traditional treatment for diabetes, ameliorates on high fructose-induced steatohepatitis and vascular inflammation. Animals were divided into four groups; Control receiving tap water, fructose-fed, rosiglitazone-treated fructose-fed rats, and MPM-treated fructose-fed rats both receiving supplemented with 60% fructose (n=10). The MPM or rosiglitazone groups initially received a high-fructose diet alone for 8 weeks, with supplementation with MPM or rosiglitazone, peroxisome proliferators-activated receptor gamma ($PPAR{\gamma}$) agonist, occurring during the final 6 weeks. Treatment with MPM significantly prevented the increase in c-reactive protein (CRP) levels in the high fructose group. MPM suppressed high fructose diet-induced vascular inflammation marker expression such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. MPM also reduced intima/media thickness of thoracic aorta. Histologic observation and oil red O staining demonstrated hepatic tissue damage and lipid accumulation were severe in high fructose group. Treatment with MPM ameliorated hepatic tissue morphology with minimized steatosis. In addition, MPM attenuated hepatitis by inhibition of monocyte chemoattractant protein-1 (MCP-1) expression. MPM-fed group showed lower serum GOT and GPT levels comparing with high fructose group. MPM and rosiglitazone (positive control) significantly decreased the size of epididymal adipocytes. Taken together, the administration of MPM inhibited high fructose-induced steatohepatitis and vascular inflammation. These results suggested that MPM is useful in the prevention or treatment of metabolic syndrome-related disorders such as fatty acid metabolism and vascular homeostasis.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.3
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• pp.349-354
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• 2014

Elevation of intracellular calcium ($Ca^{{+}{+}}$) triggers degranulation of mast cells by bypassing receptor activation. Flos Sophora japonica L. has been used as a natural dying source and has been reported to have biological activities such as anti-inflammatory and anti-allergic effects through $Fc{\varepsilon}RI$ and IgE crosslinking. In the present investigation, we report the regulatory effect of ethanolic extract of Flos Sophora japonica L. (S.F) on allergic mediators produced by $Ca^{{+}{+}}$ ionophore activation in mast cells. S.F significantly inhibited calcium ionophore (A23187)-induced interleukin (IL)-4 and tumor necrosis factor (TNF)-${\alpha}$ production as well as mast cell degranulation. Furthermore, administration of S.F suppressed allergic reactions in a 2,4-dinitrofluorobenzene (DNFB)-induced allergic dermatitis mouse model. Both oral administration and ear painting using 50 mg/kg of S.F significantly reduced levels of cytokines such as IL-4, TNF, and interferon-${\gamma}$ in ear tissues compared to the DNFB alone-treated group. Serum IgE level in the S.F-treated group also decreased compared to the DNFB alone-treated group. Weights of spleens and lymph nodes in the S.F-treated groups also decreased compared to the control group. Considering the data, we conclude that S.F mediates its anti-allergic effects not only through $Fc{\varepsilon}RI$ stimulation but also $Ca^{{+}{+}}$ influx in mast cells.

• Nuclear Engineering and Technology
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• v.4 no.4
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• pp.340-344
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• 1972

Isomeric ratios were measured for the capture of thermal neutron by $^{79}$ Br, $^{80}$ Se, $^{103}$ Rh, $^{115}$ In and $^{133}$ Cs as well as those of epi-cadmium neutron by $^{79}$ Br, $^{80}$ Se and $^{l33}$Cs. The measurements were performed by analysing decay curves obtained by ${\gamma}$-ray spectrometry after irradiation. The counting efficiency curve was determined by using the calibrated standard sources with overall uncertainties of about 1%. Isomeric ratios, given in $\sigma$ high spin/($\sigma$ high spin + $\sigma$ low spin), of $^{80, 80m}$Br, $^{81,81m}$Se, $^{014, 104m}$Rh, $^{116,116m}$In and $^{134, 134m}$Cs produced by thermal neutron activation were found to be 0.21$\pm$0.01, 0.14$\pm$0.02, 0.12$\pm$0.02, 0.69$\pm$0.07 and 0.058$\pm$0.004, respectively, Those values of $^{80, 80m}$Br, $^{81,81m}$Se, and $^{134, 134m}$Cs Produced by epi-cadmium neutron were found to be 0.19$\pm$0.02, 0.29$\pm$0.02 and 0.074$\pm$0.011, respectively. The experimental values obtained were compared with the theoretical values deduced from the statistical model. There were the general agreements between the theory and the experiment.t.

• Korean Journal of Food Science and Technology
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• v.33 no.6
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• pp.693-699
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• 2001

ChlorophyII destruction by irradiation in linoleic acid in methanol system was studied. ChlorophyII b standard (3 ppm) was added into methanol solution containing 1% of linoleic acid, and the sample was irradiated up to 20 kGy with or without nitrogen gas-bubbling. The content of chlorophyII b was analyzed by HPLC, and Hunter color value and UV-visible spectra were analyzed during 6 hr of photooxidation. The content of chlorophyll was reduced by irradiation and completely destroyed at 2.517 kGy. The model system with nitrogen gas-bubbling did not develop the lipid oxidation after irradiation or photooxidation at 20 kGy UV-visible spectra showed decreasing values of optical density by increase of irradiation doses, supporting the destruction of chlorophyII b. The results indicate that irradiation technology can be applied to reduce or eliminate the residual chlorophyII and to prolong the shelf-life of oil products.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.4
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• pp.494-500
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• 2012

In order to determine the functional benefits of $Cordyceps$ $militaris$ in the immune system, we examined the immunomodulatory activities of $Cordyceps$ $militaris$ in an immunocompromised C57BL/6 mice model. Mice were injected intraperitoneally with an immunosuppressive drug, cyclophosphamide, and then administered orally with 3% hydroxypropylmethylcellulose or 30, 100, and 300 mg/kg of 50% ethanol extract of $Cordyceps$ $militaris$ (CM 30, CM 100, and CM 300, respectively) for 12 days. Mice treated with CM displayed significantly increased splenocyte proliferation and natural killer cell activity compared to immunosuppressed control mice (p<0.05). The spleen cells isolated from mice treated with CM also displayed increased production of Th1 cytokines, including IL-2, IL-12, IFN-${\gamma}$ and TNF-${\alpha}$, suggesting enhanced cellular immunity in response to CM. However, CM had no significant effect on the production of IL-4 and IL-10. These results indicate that $Cordyceps$ $militaris$ enhances immune function by promoting immune cell proliferation and Th1 cytokine production.

• Journal of the Korean Geotechnical Society
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• v.30 no.3
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• pp.59-72
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• 2014

For circular rigid footings with a rough base on sand, combined vertical - moment loading capacity was studied by three-dimensional numerical modelling. Mohr-Coulomb plasticity model with the associated flow-rule was used for the soil. After comparing the results of the swipe loading method, which can construct the interaction diagram with smaller number of analyses, and those of the probe loading method, which can simulate the load-paths in the conventional load tests, it was found that both loading methods give similar results. Conventional methods based on the effective width or area concept and the results by eccentricity factor ($e_{\gamma}$) were reviewed. The results by numerical modelling of this study were compared with those of previous studies. The combined loading capacity for vertical (V) - moment (M) loading was barely affected by the internal friction angle. It was found that the effective width concept expressed in the form of eccentricity factor can be applied to circular footings. The numerical results of this study were smaller than the previous experimental results and the differences between them increased with the eccentricity and moment load. Discussions are made on the reason of the disparities between the numerical and experimental results, and the areas for further researches are mentioned.

• Korean Chemical Engineering Research
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• v.45 no.6
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• pp.619-626
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• 2007

It is of special interest in our membrane separation technology due to its low energy consumption and cost, relatively simple equipment, low investment and operation cost, et al. Full scale utilization of such processes can be widely utilized to the various fields. Using the difference of permeability of gas molecules between the filter layers, it is able to separate effectually pure gases from the mixed gases. In this paper, the membranes of PDMS, ${\gamma}-radiated$ PDMS, PTFE, PTFE-X are chosen to develop the predictive model for the separation of pure gases such as oxygen, nitrogen, hydrogen, and other gases from mixed gases. By utilizing the thermodynamic gas properties($\sigma$, $\varepsilon/k$) and experimental data of gas transport characteristics for different polymer membranes, it is able to develop the predictive model equation under the influence of temperature, pressure and polymer characteristics. Predictive model developed in this research showed good agreement with experimental data of gas permeability characteristics for develop four different polymer membranes. The proposed model can also be extended to the general equation for predicting the separation of gases based on the properties of polymeric membranes.

• Journal of the Korean Geotechnical Society
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• v.28 no.12
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• pp.53-64
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• 2012

In this study, the modified cyclic triaxial tests with Joomunjin standard sand are performed for dynamic deformation characteristics, such as Young's moduli and damping ratio. The cyclic triaxial test is equipped with Local Displacement Transducer (LDT) on the outside of a cell which has a range from $10^{-4}$ to $10^{-1}$ of shear strains, ${\gamma}$ (%), instead of conventional cyclic triaxial test which has linear variable displacement transducer (LVDT) with low precision. With the small strain control, tests were carried out at various loading rates, void ratios, and effective confining pressures. Based on the test results, such as dynamic deformation characteristics, shear modulus, and damping ratio, it is found that the test can measure more range of medium strains (0.02-0.2%) than results obtained from conventional test (resonant column test). For the medium strain range, dynamic deformation characteristics investigated by the cyclic triaxial test are also different from those predicted by nonlinear model in conventional test.