• Food Science and Preservation
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• v.20 no.5
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• pp.608-613
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• 2013

The physicochemical characteristics and antioxidant capacities of 15 red wines (Cabernet Sauvignon) from the US (5) and Chile (10) were investigated. The contents of soluble solid, reducing sugar, titratable acidity, and $SO_2$ were measured. Antioxidant capacities were examined by DPPH, ORAC assay, and total phenolic contents. In addition, polyphenols composition were analyzed by HPLC. The contents of soluble solid, reducing sugar, and acidities were 7.03~8.6 $^{\circ}Brix$, 2.7~6.7 g/L, and 0.7~0.8%, respectively, and showed no differences between wines from the two countries (p<0.05). The $SO_2$ content of Chile wines was 50% higher than that of US wines (p<0.05). Antioxidant activities by DPPH assay ranged 5.58~9.80 mM and 6.77~9.48 mM in the US and Chile wines, respectively. The ORAC values of the US and Chile wines ranged 2.17~18.08 mM and 4.55~33.77 mM, respectively. The total phenolic content ranged from 1,315 to 2,651 mg/L among the US red wines, and from 1,653 to 2,493 mg/L among Chile red wines. Gallic acid, catechin, syringic acid, p-coumaric acid, quercetin, and kaempferol were identified by HPLC. The polyphenol contents of the Chile red wines were higher than those of the US red wines(p<0.05). There were no differences in the physicochemical characteristics and the antioxidant capacities of the wines from US and Chile, but only in their $SO_2$ and polyphenol contents (p<0.05).

• The Korean Journal of Mycology
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• v.24 no.3 s.78
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• pp.214-222
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• 1996

This study was carried out to obtain the basic data on artificial culture of Phellinus linteus. The optimum condition for the mycelial growth was $25{\sim}30^{\circ}C$ and pH $6.0{\sim}7.0$. The carbon sources such as D-glucose, D-mannose and Dextrose were favorable to mycelial growth. As nitrogen sources, peptone, cassamino acid and glutamic acid appeared to be favorable. The optimum C/N ratio was about 20:1, when 2% of glucose was provided as a carbon source. The better organic acids and vitamin among tested ones were gallic, silicic acids and biotin. The mineral nutrients of $KH_2PO_4,\;FeSO_4{\cdot}7H_2O,\; MgSO_4{\cdot}7H_2O,\;ZnSO_4{\cdot}7H_2O$ were effective and the optimum concentrations were 0.05, 0.001, 0.02 and 0.003%, respectively.

• Journal of Korean Society of Forest Science
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• v.85 no.2
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• pp.244-250
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• 1996

The objectives of this paper were to study the existence of resistant substance to pine gall midge from needles of Pinus densiflora, P. thunbergii, P. virginiana, and P. thunbergii${\times}$P. virginiana, and also to investigat whether terpenoids, fragrant substance contained in those species seduce or evade imago of pine gall midge to oviposit. Also, terpenoids and phenolic compounds were bioassayed on larva. The results are follows. Adults of pine gall midge oviposited indiscriminately all investigated pine species, while gall formation rate by pine gall midge showed 0% in Pinus virginiana, 9% in P. thunbergii${\times}$P. virginiana, 22% in P. thunbergii. It is suggested that young larvae is necrotized by resistant substance in the needles of P. virginiana. This results might mean that fragrant substance, terpenoids, extracted from pine species is not seducible or evadable substance. Larvae of pine gall midge placed on terpenoid and resin were not necrotized. The necrosis rate of larvae of pine gall midge cultivated in the solution of phenolic compounds extracted from needles of P. virginiana, salicylic acid and chitinase showed 89, 92, and 86% respectively. And necrosis rate was 56 and 59% in phenolic compounds extracted from P. densiflora and gallic acid respectively. So, it is postulated that phenolic compounds contained in needles of P. virginiana, salicylic acid and chitinase are resistant substance to pine gall midge.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.11
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• pp.1343-1349
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• 2017

This study was carried out to determine the simultaneous extraction conditions of functional components (lutein, ${\beta}$-carotene, total polyphenol, flavonoids, and phenolic compounds) from sweet potato leaves and to evaluate the antioxidant activities. Extraction conditions included different ethanol concentrations (1st extraction: 99.9% ethanol; 2nd extraction: 50~90% ethanol) and times (30, 60, and 90 min). The highest values of lutein and ${\beta}$-carotene content were obtained by the 2nd extraction at an ethanol concentration of 90%. The extraction yields of lutein and ${\beta}$-carotene decreased with increasing extraction time. The maximum polyphenol, flavonoid, and total phenolic acid contents and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activities were 32.3 mg gallic acid equivalent/g, 17.0 mg catechin equivalent/g, 2,842.6 mg/100 g, 17.0 mg ascorbic acid equivalent/g, and 1.94 mg/mL ($IC_{50}$) at the 2nd extraction with an ethanol concentration of 60%. The optimum extraction conditions were as follows; ethanol concentrations of the extraction solvent were 99.9% (1st extraction) and 60% (2nd extraction), and extraction time was 30 min.

• Journal of Life Science
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• v.28 no.12
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• pp.1461-1468
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• 2018

The Alaska Pollock (Gadus chalcogrammus) is distributed in an arc across the North Pacific Ocean. Distilled water extracts (DWE) and ethanol extracts (ETE) of 1.0 mg/ml concentrations of raw Alaska Pollock roe, premium Gochujang Pollock roe, and premium fermented Pollock roe seasoning were evaluated for estimated 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), ferric reducing antioxidant potential (FRAP), and total phenolic content. The ABTS scavenging activity of the raw Alaska roe DWE and ETE were evaluated at 50.1% and 53.1%, respectively. The ABTS scavenging activity of the Gochujang roe DWE was 68.7% and of the ETE was 70.4%; for the fermented seasoning it was 71.3% and 71.6% for the DWE and ETE, respectively. The ABTS $EC_{50}$ values of the raw roe DWE and ETE were 12.49 ug/ml and 12.21 ug/ml, respectively. The FRAP $EC_{50}$ values of the Gochujang roe DWE and ETE were 10.67 ug/ml and 10.56 ug/ml, respectively, and the $EC_{50}$ values for total phenolic content for the fermented seasoning DWE and ETE were 10.45 ug/ml and 10.31 ug/ml, respectively. When Gallic was acid used as a control, the relative total phenolic content scavenging activity in each ETE was 52.0% (raw Alaska roe), 61.1% (Gochujang roe), and 63.6% (fermented seasoning). In the present study, higher ABTS, FRAP, and total phenolic content were observed in the Gochujang Pollock roe and fermented roe seasoning than in the Alaska Pollock roe.

• Journal of the Korean Wood Science and Technology
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• v.34 no.2
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• pp.95-100
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• 2006

This study is to isolate bio-active compounds from Alnus firma and evaluate their antioxidant activity. Dried wood powder of A. firma was extracted by organic solvents and fractionated in the sequential extraction steps. The isolated compounds were characterized by EI-MS, $^{13}C-$ and $^1H-NMR$ including COSY, DEFT, HMQC, and HMBC. Antioxidant activities of the isolated compounds were evaluated by DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging effect. From the wood of A. firma, three kinds of diarylheptanoids, alnusodiol (1), alnusonol (2) and alnusone (3), and gallic acid (4) were isolated. Among these four compounds, compound 1, 2, and 3 are isolated from A. firma for the first time. The antioxidant activity of gallic acid was 93.5% at the concentration of 100 ppm. This compound showed stronger antioxidant activity than those of other isolated compounds and the reference BHT (butylated hydroxytoluene).

• Preventive Nutrition and Food Science
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• v.2 no.4
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• pp.285-290
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• 1997

Previously, a 50% aqueous methanol extract of Galla rhois was shown to be the most potent tyrosinase inhibition activity with an {TEX}$IC_{50}${/TEX}(the concentration causing 50% inhibition of tyrosinase activity) of 0.2mg/ml of 205 crude drug extracts. To isolate tyrosinase inhibitors, the methanol extract was evaporated to a small volume in vacuo, and then partitioned stepwise with benzene and ethyl acetate(EtOAc). the EtOAc fraction was solubilized in 10% MeOH solution, and then fractionated successively by Diaion HP-20 and Sephadex LH-20 column chromatography, and preparative HPLC. Three phenolic compounds were isolated, and characterized as gallic acid(GA), methyl gallate(MG) and 1,2,3,4,6-penta-O-galloyl-$\beta$-D-glucose(PGG) by UV, IR, {TEX}${1}^H${/TEX}-&{TEX}${13}^C${/TEX}-NMR, and FAB-MS spectroscopy, PGG({TEX}$IC_{50}${/TEX}=50$\mu\textrm{g}$/ml) showed a considerable inhibitory effect against mushroom tyrosinase, while GA({TEX}$IC_{50}${/TEX}=1.6mg/ml) and MG({TEX}$IC_{50}${/TEX}=234$\mu\textrm{g}$/ml) did not show an appreciable effect. Meanwhile, MG inhibited greatly melanogenesis in a murine melanocyte cell line, Mel-Ab. MG and PGG showed typical noncompetitive inhibition patterns against mushroom tyrosinase. These results suggest that PGG and MG may be potentially useful as either anti-browning or anti-melanogenic agents in foods and cosmetics.

• Journal of Applied Biological Chemistry
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• v.60 no.2
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• pp.185-190
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• 2017

Antioxidative ability of 50% ethanol extract from leaves of Leucaena leucocephala (Lam.) de Wit was investigated. The extraction yield, total polyphenols and total flavonoids of the ethanol extract were $18.3{\pm}0.40%$, $121.3{\pm}11.04{\mu}g$ gallic acid equivalents/mg and $105.2{\pm}2.77{\mu}g$ quercetin equivalents/mg, respectively. The ethanol extract possessed the antioxidative activities such as free radical, cation radical, and nitrite scavenging activities as well as reducing power and lipid peroxidation inhibitory activity. These antioxidative activities of the ethanol extract increased in proportion to the extract concentration. $EC_{50}$ values for free radical scavenging, cation radical scavenging nitrite scavenging, and reducing power were $133.51{\mu}g/mL$, $667.22{\mu}g/mL$, 2.58 mg/mL, and $250.00{\mu}g/mL$, respectively. Polyphenols and flavonoids are suggested to be the key contributors for the antioxidative ability of the ethanol extract from Leucaena leucocephala leaves.

• Mycobiology
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• v.36 no.1
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• pp.60-65
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• 2008

The effect of methanolic extract of cashewnut (Anacardium occidentale) shell extract was seen on conidial germination of Erysiphe pisi and powdery mildew development in pea (Pisum sativum). Maximum conidial germination inhibition of E. pisi on glass slides was observed at 300 ppm. Similar effect on floated pea leaves was observed after 48 h at the same concentration. Conidial germination on intact untreated pea leaves was also assessed on II and IV nodal leaves while IV and II nodal leaves were treated with the extract and vice versa. There was tremendous reduction in conidial germination on all the nodal leaves. The disease intensity of pea powdery mildew was significantly reduced by methanolic extract of cashewnut shells. Maximum reduction was observed with 200 ppm where 39% disease intensity was recorded in comparison to 96.53% in the control. The phenolic acid content of pea leaves following treatments with this extract varied and no defmite pattern was observed. Out of several phenolic compounds, namely, gallic, ferulic, chlorogenic, and cinnamic acids, only gallic acid was found to be present consistently in all the treatments with varied amounts.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.143.2-144
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• 2003

품종별 감잎의 주요성분과 항산화 활성을 비교하여 고기능성 감잎차의 우수성을 알리는 기초자료로 활용하고자 이 실험을 수행하였다. 상주둥시, 갑주백목, 월하시, 청도반시, 녹차(대조구)의 생엽을 7월 초순에 채엽 후 건조시켜 시료로 사용하였고, 비타민C, 카페인, 카테친, Gallic acid, 향기성분, 유리당, 유리아미노산, 칼슘, 항산화 활성을 조사하였다. 비타민C는 월하시 0.089%, 청도반시 0.079%, 갑주배목 0.032%, 상주둥시 0.015%, 녹차 0.01%로 비타민C 함량이 가장 많은 월하시가 녹차에 비해 8배정도 높았고, 카페인은 감잎 4품종 모두 발견되지 않았고, 녹차는 6.63%이었다. 카테친은 청도반시 0.35%, 갑주백목 0.34%, 월하시 0.24%, 상주둥시 0.18%, 녹차(대조구) 0.07%이였고, Gallic acid는 상주 둥시 0.32%, 갑주백옥 0.2%, 월하시 0.05%, 청도반시 0.03%, 녹차(대조구) 1.41%이었다. 칼슘은 청도반시 9516.1PPM, 영동월하시 6863.5PPM, 봉옥 6563.5PPM, 상주둥시 5420.1PPM, 녹차(대조구) 2349.7PPM이였고, 유리당은 감품종간에는 큰 차이는 없었으나 녹차에 비해 Xylose, fructose, Glucose, Sucrose의 함량이 높았고, Maltose의 함량에 일어서는 녹차가 높았다. 유리아미노산은 31항목을 조사하였고, 전체 함량은 상주둥시 60.40, 봉옥 53.21, 월하시 52.29, 청도반시 47.58, 녹차(대조구) 114.72nmo1/${\mu}\ell$이었다. 시료의 향기성분은 생엽을 건조시켜 전자코를 이용하여 분석하였고, 감잎 품종간에는 향기 패턴이 비슷한 경향이였으나, 녹차의 패턴과는 차이가 있었다. 감잎의 DPPH radical 소거활성은 상주둥시 RC$_{50}$($\mu\textrm{g}$)=64.5 청도반시 64.0, 월하시 42.0, 갑주백목 47.0, 녹차(대조구) 19.0으로 김잎품종중 월하시의 항산화 활성이 높았고, 녹차의 항산화 활성이 감잎에 비해 높았다.