• 한국동물위생학회지
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• 제33권2호
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• pp.207-211
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• 2010

None of the numerous published canis idiogram and karyotypes has yet been generally accepted as a standard one because the dog has 76 acrocentric autosomes of similar size and shape. The karyotypes of DongGyeongi dog were analysed by conventional trypsin/Giemsa staining (GTG-banding techniques), and were compared with one another. There were no variations in karyotypes which were analysed by conventional GTG-banding techniques, but differences were observed in G-banding patterns with sapsaree (or canis familiaris strains). It is not clear that these disagreements in G-banding patterns between strains of dog were caused by chromosome polymorphism or a difference in interpretation.

• 생명과학회지
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• 제12권5호
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• pp.605-609
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• 2002

개의 염색체수는 2n=78이며 염색체의 형태를 보면 submetacentric chromosome metacentric chromosome인 X와 Y를 제외하고 모든 상염색체가 acrocentric chromosome이며 그 크기가 작고 크기의 차이가 나지 않는 염색체들이 많아 G-banding등에 의한 핵형의 표준화가 완전하게 이루어지고 있지 않다. 본 연구fl서는 G-banding high-resolution-banding NOR- banding등의 방법을 이용하여 개의 미소 염색체들에 대한 핵형 및 염색체 동정을 하고자 하였다. C-banding과 high-resolution banding에 의한 그 결과 아직 표준화가 되어 있지 않은 염색체 중 34번, 37번 등의 염색체에서 본 연구 결과가 차이가 있었으나 이는 해석상의 차이로 보이며 NOR-banding에 의한 분석결과 Y염색체와 3쌍의 상염색체에서 존재함을 확인 할 수 있었고 이는 개의 염색체 동정의 지표로 활용할 수 있으리라고 생각된다.

• 한국동물위생학회지
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• 제34권3호
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• pp.291-296
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• 2011

The karyotype of the domestic dog is widely accepted as one of the difficult mammalian karyotypes to work. In contrast to many other animals, knowledge about the canine karyotype is quite sparse. The dog has a total of 78 chromosomes; all 76 autosomes are acrocentric in morphology and show only a gradual decrease in length. But appear to be quite small and difficult to identify unambiguously. To purchased standardization of chromosome in Korea native dog, there were analyzed by conventional trypsin/Giemsa staining (GTG-banding techniques), and were compared with 4, 6, 8, 11, 13, 17 chromosome. There were no variations in karyotypes which were analyzed by conventional GTG-banding techniques, but differences were observed in G-banding patterns with Sapsaree, Jindo, Gyeongju DongGyeong dogs, Welshi-Corgi. It is not clear that these disagreements in G-banding patterns between strains of dog were caused by chromosome polymorphism or a difference in interpretation. Comparative analysis of the distribution patterns of conserved segments defined by dog paints in the genomes of the Korea native dogs demonstrates that their differences in the karyotypes of these three species could have resulted from acrocentric banding patterns.

• 대한수의학회지
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• 제44권2호
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• pp.159-162
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• 2004

We investigated the cytogenetic characteristics of male black-striped field mouse (Apodemus agrarium) in Korea. Chromosome slides were obtained from blood cell cultures which were synchronized with thymidine blocking or not. In the chromosome slide which synchronization with thymidine blocking was employed on, the GTG(G bands by trypsin using Giemsa)-bands of high resolution were observed. The male black-striped field mouse has 48 chromosomes composed 46 autosomes and XY sex chromosomes. The centromeric regions of autosomes were positive to GTG-banding. According to this investigation, thymidine blocking in cell culture process was useful to get lengthened chromosomes. It may be necessary to employ RBG-banding technique to investigate complementary band patterns between R- and G-banding in black-striped field mouse.

• Journal of Genetic Medicine
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• 제2권2호
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• pp.71-77
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• 1998

Comparative genomic hybridization (CGH) can now be applied to detect the origin of extra or missing chromosomal material in cases with common unbalanced aberrations and in prenatal investigations. This method has been used in 13 cases of fetal samples for this study; 3 for amniocytes, 2 for cord blood and 8 for abortus tissues. These samples were previously subjected to GTG-banding. Our study showed aneuploidy in 8 cases, and partial monosomy, partial trisomy or marker chromosome in the remaining 5. The CGH disclosed further small genetic imbalances in 4 of all 13 cases: a prenatal sample showing del(20)(q13) by GTG confirmed a loss of the segment 20p13-pter by CGH; a marker chromosome manifested normal CGH profile; chromosome der(?)(?;15) found in an abortus sample by GTG turned out to be a loss of 15pter-q14 (partial monosomy) and a gain of 10pter-q22 (partial trisomy); the der(15) shown by GTG represented partial trisomy of 3q24-qter. These findings show that CGH is very useful and efficient for cytogenetic investigations of clinical cases.

• Journal of Animal Science and Technology
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• 제45권1호
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• pp.1-12
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• 2003

본 연구에서는 국내 고유 재래 가축들의 유전정보와 유전학적 개량의 기초자료 제공을 목적으로 고분염 분석(high-resolution banding) 방법에 의한 한국재래계의 염색체 분염 표지를 제시하였다. 본 연구에 공시된 한국재래계로서는 축산기술연구소에서 계통화 시킨 황갈색 및 적갈색 계통으로 이들이 생산한 수정란의 초기 배자를 이용하여 염색체 분석을 수행하였으며 닭의 초기배자에 EtBr 및 colchicine을 처리함으로써 보다 양호한 고정도 염색체를 획득하였다. 한국재래계의 GTG-banding 결과 모든 상동염색체간에 뚜렷하고 특징적인 band 양상을 얻을 수 있었으며, Leghorn 및 국제표준핵형(ISSAK)과 비교시 염색체의 형태적 양상에서는 거의 차이가 없는 것으로 분석되었고 대표적 landmark간에도 거의 일치되는 양상을 보였다 그러나 대부분의 한국재래계의 대형염색체에서 더 많은 G-band의 분리 양상을 보이고 특히 1번 및 Z 염색체에서 특징적 분리 양상의 차이를 보였다. 한국재래계의 C-banding 분석에서는 세포별 heterochromatin의 다형성을 보이기는 하나 대부분의 염색체의 동원체와 말단부위에서 C- band가 나타났으며, Z 염색체 장완 말단부와 W 염색체 전체에서는 거의 모든 세포에서 C-band가 출현하였다. 또한 3번 염색체 동원체와 Z 염색체 장완 말단부에서 특징적 다형성을 나타내어 이들 염색체들에서는 상동염색체간 heterochromatin의 이형적 양상(heteromorphic)이 존재함을 밝혔다.

• Plant Resources
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• 제5권3호
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• pp.169-175
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• 2002

The soybean cyst nematode (Heterodera glycines Inchinoe; SCN) is a devastating pest of soybean and is responsible for significant losses in yield. The use of resistant cultivars is the effective method to reduce or eliminate SCN damage. The objective of this research is to identify AFLP markers linked to the SCN resistant genes. Bulked genomic DNA was made from resistant and susceptible genotypes to SCN and a total of 19 primer combinations were used. About 31 fragments were detected per primer combination. The banding patterns were readily distinguished in resistant and susceptible bulked genotypes. Polymorphic fragments were detected between resistant and susceptible bulked genotypes in the primer combination of CGT/GGC, CAG/GTG and CTC/GAG. In primer combinations of CGT/GGC and CAG/GTG, bulked resistant genotype produced a polymorphic bands. However, in primer of CTC/GAG, bulked susceptible genotype produced a polymorphic fragments. Three AFLP markers identified as a polymorphic fragments between bulked genomic DNA were mapped in 85 F2 population. Among them, only two markers, CGT/GGC and CTC/GAG, was linked and was mapped. Broad application of AFLP marker would be possible for improving resistant cultivars to SCN.

• Clinical and Experimental Pediatrics
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• 제45권6호
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• pp.804-808
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• 2002

저자들은 폐형성부전과 다발성 선천성 기형을 가지고 있는 생존 출생아에서 말초혈액의 염색체 검사 및 FISH로 확인된 trisomy 22 1례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.

• Reproductive and Developmental Biology
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• 제30권4호
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• pp.263-270
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• 2006

The Chinese Hamster Ovarian cells CHO-K1 are one of the most extensively used cells for the evaluation of gene expression and toxicology. However, these cells are frequently used for biomedical research without consideration of their cytogenetic characteristics. Therefore, we carried out to investigate the karyologic profiles, the frequency and type of chromosome aberration, and the distribution of telomeric DNA on chromosomes of the CHO-K1 cells. The GTG banding and fluorescence in situ hybridization on CHO-K1 cells were performed to characterize the karyotype and the distribution of telomeric DNA The present study revealed that the chromosome modal number of CHO-K1 cells was 2n=20; eight chromosomes appeared to be identical with those of the normal Chinese hamster, whereas the remaining 12 chromosomes were shown to be translocated, deleted, inversed, or rearranged from Chinese hamster chromosomes. The telomeric DNA on CHO-K1 chromosomes was intensively distributed at the centromeres rather than the ends of chromosomes. In addition, three chromosomes had interstitial telomeres and one marker chromosome entirely consisted of telomeric DNAs. The frequency and type of chromosome aberrations in CHO-K1 cells were examined. Of the 822 metaphase spreads, 68 (8.3%) cells resulted in chromosome aberrations of which the chromosome breakage was the most frequently occurred.

• Clinical and Experimental Reproductive Medicine
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• 제22권2호
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• pp.109-122
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• 1995

A technique is described for producing high resolution G- and R-banded chromosomes in human peripheral lymphocyte cultures. Cultured lymphocyte cells were exposed to ethidium bromide ($10{\mu}g/ml$) and colcemid ($0.02{\mu}g/ml$) each for 2.5h and 0.5h prior to harvest for high resolution G-banded chromosomes. High resolution R-band patterns were obtained by BrdU substitution which was revealed by the fluorochrome-photolysis-Giemsa staining technique. These methods are easy to perform and highly reliable. The data on relative length of chromosomes at the four mitotic stages are presented in units of percentage of haploid autosome length. The characteristic patterns of GTG-bands (G-bands after trypsin and Giemsa) and RBG bands (R-bands after BrdU and Giemsa) were analyzed.