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Chromosomal band pattern of black-striped field mouse (Apodemus agrarius)  

Oh, Seung-hyun (Laboratory of Departmental Biology and Genomics, College of Veterinary Medicine, Seoul National University)
Yoon, Yeo-sung (Laboratory of Histology, College of Veterinary Medicine, Seoul National University)
Jin, Hee-Kyung (Laboratory of Laboratory Animal Medicine, College of Veterinary Medicine, Kyungpook National University)
Seong, Je-kyung (Laboratory of Departmental Biology and Genomics, College of Veterinary Medicine, Seoul National University)
Publication Information
Korean Journal of Veterinary Research / v.44, no.2, 2004 , pp. 159-162 More about this Journal
Abstract
We investigated the cytogenetic characteristics of male black-striped field mouse (Apodemus agrarium) in Korea. Chromosome slides were obtained from blood cell cultures which were synchronized with thymidine blocking or not. In the chromosome slide which synchronization with thymidine blocking was employed on, the GTG(G bands by trypsin using Giemsa)-bands of high resolution were observed. The male black-striped field mouse has 48 chromosomes composed 46 autosomes and XY sex chromosomes. The centromeric regions of autosomes were positive to GTG-banding. According to this investigation, thymidine blocking in cell culture process was useful to get lengthened chromosomes. It may be necessary to employ RBG-banding technique to investigate complementary band patterns between R- and G-banding in black-striped field mouse.
Keywords
black-striped field mouse; karyotype; GTG-bands;
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1 Koh, H. S. G- and C- banding pattern analysis of Korean rodents. Korean J. Zool. 1982, 25, 81-92
2 Lee, H. W., Lee, P. W. and Johnson, K. M. Isolation of the etiologic agent of Korean hemorrhagic fever. J. Infect. Dis. 1978, 137, 298-308
3 김윤희, 김희수, 최영현, 이원호. 한국산 등줄쥐의 체세포 및 생식세포 분열에 의한 염색체 분석. 한국실험동물학회지. 2001, 17, 17-26
4 조정식, 채갑용, 김지희. 한국에서 서식하는 야생들줄쥐의 실험동물화에 관한 연구(II). 과학기술처, 1992
5 조정식, 채갑용, 김지희. 한국에서 서식하는 야생등줄쥐(Apodemus agrarius Pallas)의 실험동물화에 관한 연구(III). 과학기술처, 1993
6 김재연, 조정식, 채갑용. 한국에 서식하는 야생등줄쥐의 실험동물화에 관한 연구(I). 과학기술처, 1991
7 오승현, 윤영민, 윤여성, 이준섭, 이흥식, 성제경. 한국재래산양의 핵형분석. 대한수의학회지. 1999, 39, 908-920
8 土屋公幸. 新しく實驗動物として開發された野生齧齒類の維持方法の開發. 九州實驗動物研究會會報, 1991, 7, 3-9
9 Oh, S. W., Chae, C. and Jang, D. Spontaneous gastric carcinoid tumors in the striped field mouse (Apodemus agrarius). J. Vet. Med. Sci. 1997, 59, 703-706
10 Takagi, N. A simple technique to demonstrate the centromeric 'heterochromatin' in the mouse and other animals. Jpn. J. Genetics. 1971, 46, 361-363
11 Latt, S. A. Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes.Proc. Natl. Acad. Sci. 1973, 70, 3395-3399
12 Di, Berardino. D., Ronne, M., Burguete, I., Lioi, M. B., Taibi, L. and Matassino, D. R-banding pattern of the prometaphase chromosomes of the goat. Heredity.1987, 78, 225-230
13 Perry, P. and Wolff, S. New Giemsa method for differential staining of sister chromatids. Nature. 1974, 261, 156-158
14 Lemieux, N., Drouin, R. and Richer, C. L. Highresolution dynamic and morphological G-bandings (GBG and GTG): a comparative study. Hum. Genet.1990, 85, 261-266