Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Cytogenetic Characteristics of Chinese Hamster Ovarian Cell CHO-K1

  • Sohn, Sea-Hwan (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Cho, Eun-Jung (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Jang, In-Surk (Department of Animal Science and Biotechnology, Jinju National University)
  • Published : 2006.12.31
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Abstract

The Chinese Hamster Ovarian cells CHO-K1 are one of the most extensively used cells for the evaluation of gene expression and toxicology. However, these cells are frequently used for biomedical research without consideration of their cytogenetic characteristics. Therefore, we carried out to investigate the karyologic profiles, the frequency and type of chromosome aberration, and the distribution of telomeric DNA on chromosomes of the CHO-K1 cells. The GTG banding and fluorescence in situ hybridization on CHO-K1 cells were performed to characterize the karyotype and the distribution of telomeric DNA The present study revealed that the chromosome modal number of CHO-K1 cells was 2n=20; eight chromosomes appeared to be identical with those of the normal Chinese hamster, whereas the remaining 12 chromosomes were shown to be translocated, deleted, inversed, or rearranged from Chinese hamster chromosomes. The telomeric DNA on CHO-K1 chromosomes was intensively distributed at the centromeres rather than the ends of chromosomes. In addition, three chromosomes had interstitial telomeres and one marker chromosome entirely consisted of telomeric DNAs. The frequency and type of chromosome aberrations in CHO-K1 cells were examined. Of the 822 metaphase spreads, 68 (8.3%) cells resulted in chromosome aberrations of which the chromosome breakage was the most frequently occurred.

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References

  1. Bertoni L, Attolini C, Simi S, Giulotto E (1992): Localization of the Chinese hamster CAD gene reveals homology between human chromosome 2p and Chinese hamster 7q. Genomics 16:779-781 https://doi.org/10.1006/geno.1993.1267
  2. Bertoni L, Attolini C, Tessera L, Mucciolo E, Giulotto E (1994): Telomeric and nontelomeric (TTAGGG)n sequences in gene amplification and chromosome stability. Genomics 24:53-62 https://doi.org/10.1006/geno.1994.1581
  3. Biondi O, Andreozzi L, Amoruso S, Motta S (2000): Phenolphthalein induces chromosome aberrations in human and Chinese hamster liver cells (CHEL) cultured in vitro. Teratog Carcinog Mutagen 20:209-217 https://doi.org/10.1002/1520-6866(2000)20:4<209::AID-TCM3>3.0.CO;2-U
  4. Burkholder GD, Duczek LL (1980): Proteins in chromosome banding. Chromosoma 79:29-41 https://doi.org/10.1007/BF00328470
  5. Comings DE (1978): Mechanisms of chromosome banding and implications for chromosome structure. Ann Rev Genet 12:25-46 https://doi.org/10.1146/annurev.ge.12.120178.000325
  6. Deaven LL, Petersen DF (1973): The chromosomes of CHO, an aneuploid Chinese hamster cell line: G-Band, C-Band, and autoradiographic analyses. Chromosoma 41:129-144 https://doi.org/10.1007/BF00319690
  7. Delany ME, Daniels LM, Swanberg SE, Taylor HA (2003): Telomeres in the chicken: Genome stability and chromosome ends. Poultry Sci 82:917-926 https://doi.org/10.1093/ps/82.6.917
  8. Engelhardt M, Martens UM (1998): The implication of telomerase activity and telomere stability for replicative aging and cellular immortality. Oncol Rep 5: 1043-1052
  9. Faravelli M, Azzalin CM, Bertoni L, Chernova O, Attolini C, Mondello C, Giulotto E (2002): Molecular organization of internal telomeric sequences in Chinese hamster chromsomes. Gene 283:11-16 https://doi.org/10.1016/S0378-1119(01)00877-0
  10. Grillari J, Fortschegger K, Grabherr RM, Hohenwarter O, Kunert R, Katinger H (2001): Analysis of alterations in gene expression after amplification of recombinant genes in CHO cells. J Biotechnol 87:59-65 https://doi.org/10.1016/S0168-1656(00)00431-4
  11. Holmquist GP, Comings DE (1976): Histones and Gbanding of chromosomes. Science 193:599-602 https://doi.org/10.1126/science.959822
  12. ISCN (1995): An International System for Human Cytogenetic Nomenclature. S. Karger Pub. Inc, Farmmington, CT
  13. Kang BC, Lee JS, Lee JW, Nam JS, Che JH, Lee SM, Yang JM, Lee HM, Park JH, Lee YS (1997): The chromosomal aberration test of YH1885 in Chinese hamster ovary cell in vitro. Korean J Lab Anim Sci 13:109-111
  14. Kao FT, Puck TT (1975): Mutagenesis and genetic analysis with Chinese hamster auxotrophic cell markers. Genetics 79(Suppl):343-252
  15. Kao FT, Puck TT (1970): Genetics of somatic mammalian cells: linkage studies with human-Chinese hamster cell hybrids. Nature 228:329-332 https://doi.org/10.1038/228329a0
  16. Kao FT, Puck TT (1969): Genetics of somatic mammalian cells. IX. Quantitation of mutagenesis by physical and chemical agents. J Cell Physiol 74:245-258 https://doi.org/10.1002/jcp.1040740305
  17. Kim SJ, Lee GM (1999): Cytogenetic analysis of chimeric antibody-producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnology and Bioengineering 64:741-749 https://doi.org/10.1002/(SICI)1097-0290(19990920)64:6<741::AID-BIT14>3.0.CO;2-X
  18. Krejci K, Koch J (1998): Improved detection and comparative sizing of human chromosomal telomere in situ. Chromosoma 107:198-203 https://doi.org/10.1007/s004120050297
  19. Lavoie J, Bronsard M, Lebel M, Drouin R (2003): Mouse telomere analysis using an optimized primed in situ (PRINS) labeling technique, Chromosoma 2003 Apr;111(7):438-44 https://doi.org/10.1007/s00412-002-0225-1
  20. Paris Conference (1972): Standardization in human cytogenetics. Cytogenetics 11:313-362 https://doi.org/10.1159/000130202
  21. Park SD, Lee BJ (1984): Effects of alkylating agents on G-banding patterns and stage sensitivity in chromosomes of Chinese hamster ovary cells in culture. Korean J Genetics 6:91-100
  22. Reading Conference (1980): Proceeding of the first international conference for the standardization of banded karyotypes of domestic animals. Hereditas 92:145-162 https://doi.org/10.1111/j.1601-5223.1980.tb01688.x
  23. Schlatter S, Stansfield SH, Dinnis DM, Racher AJ, Birch JR, James DC (2005): On the optimal ratio of heavy to light chain genes for efficient recombinant antibody production by CHO cells. Biotechnol Prog 21:122-133 https://doi.org/10.1021/bp049780w
  24. Shay JW, Wright WE (2001): Ageing and cancer: the telomere and telomerase connection. Novartis Found Symp 235:116-125
  25. Shibasaki Y, Ronne M (1988): Banding studies in Cricetulus griseus Milne-Edwards, 1867. I. High-resolution banded karyotypes from primary cultures. Cytogenet Cell Genet 49:282-284 https://doi.org/10.1159/000132678
  26. Slijepcevic P (2001): Telomere length measurement by Q-FISH. Methods Cell Sci 23:17-22 https://doi.org/10.1023/A:1013177128297
  27. Slijepcevic P, Bryant PE (1995): Absence of terminal telomeric FISH signals in chromosomes from immortal Chinese hamster cells. Cytogent. Cell Genet 69:87-89 https://doi.org/10.1159/000133944
  28. Sohn SH, Lee CY, Ryu EK, Han JY, Multani AS, Pathak S (2002a): Rapid sex identification of chicken by fluorescence in situ hybridization using a W chromosome-specific DNA Probe. Asian-Aust J Anim Sci 15:1531-1535 https://doi.org/10.5713/ajas.2002.1531
  29. Sohn SH, Multani AS, Gugnani PK, Pathak S (2002b): Telomere erosion-induced mitotic catastrophe in continuously grown Chinese hamster Don cells. Exp Cell Res 279:271-276 https://doi.org/10.1006/excr.2002.5614
  30. Thompson LH (1983): The use of DNA-repair-deficient mutants of Chinese hamster ovary cells in studying mutagenesis mechanisms and testing for environmental mutagens. Basic Life Sci 23:217-246
  31. Wang HC, Federoff S (1974): Trypsin technique to reveal G-bands: Tissue Culture Methods and Applications. Academic Press. New York