• Title/Summary/Keyword: GS1

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High Molecular Weight Glutenin Subunit in Common Wheat (Triticum aestivum L.) (밀의 고분자 글루테닌 단백질)

  • Lee, Jong-Yeol;Kim, Yeong-Tae;Kang, Chon-Sik;Lim, Sun-Hyung;Ha, Sun-Hwa;Ahn, Sang-Nag;Kim, Young-Mi
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.479-489
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    • 2011
  • Gluten is the main functional component of wheat, and is the main source of the viscoelastic properties in a dough. One of the gluten group is glutenin, which is composed of high molecular weight (HMW) and low molecular weight (LMW) subunits. The HMW glutenin subunits (HMW-GS) have been shown to play a crucial role in determining the processing properties of the grain. They are encoded by the Glu-1 loci located on the long arms of homeologous group one chromosomes, with each locus comprising two genes encoding x- and y-type subunits. The presence of certain HMW subunits is positively correlated with good bread-making quality. The highly conserved N- and C- terminal contaning cystein residues which form interand intra-chain disulphide bonds. This inter chain disulphide bonds stabilize the glutenin polymers. In contrast, the repetitive domains that comprise the central part of the HMW-GS are responsible for the elastic properties due to extensive arrays of interchain hydrogen bonds. In this review, we discuss HMW-GS, HMW-GS structure and gluten elasticity, relationship between HMW-GS and bread wheat quality and genetic engineering of the HMW-GS.

The Effect of Dual-Task Exercise on an Unstable Surface on Involuntary Arm and Leg Movement and Balance in Stroke Patients (불안정한 지지면에서 과제운동이 뇌졸중 환자의 팔과 다리의 불수의적 움직임과 균형에 미치는 영향)

  • Kang, Jeong-Il;Baek, Seung-Yun;Jeong, Dae-Keun
    • Journal of the Korean Society of Physical Medicine
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    • v.17 no.1
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    • pp.85-92
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    • 2022
  • PURPOSE: This study aimed to analyze the effects of task exercise on an unstable surface on the involuntary arm and leg movement and balance in stroke patients. METHODS: 20 stroke patients were clinically sampled and randomly assigned to two groups of 10 patients each. Experimental group I received task exercise intervention on an unstable surface and experimental group II received task exercise intervention. The interventions were carried out 3 times a week for 4 weeks (30 minutes per session). The global synkinesis (GS) and Berg Balance Scale (BBS) of the patients were evaluated as pre-tests prior to intervention, and then the post-tests were re-measured in the same manner as the pre-tests after a 4-week intervention period. RESULTS: In the experimental group I, there were statistically significant differences in the changes in the arm and leg GS (p < .01) and in the BBS (p < .05). On the other hand, in the experimental group II, there were statistically significant differences in the changes only in the arm GS (p < .01) and in the BBS (p < .05). Also, in the comparison of the changes between the two groups, there was a statistically significant difference in the changes in the leg GS only (p < .05). CONCLUSION: In the experimental group I, the arm and leg GS were statistically significantly reduced, but the balance ability was statistically significantly improved. The reason was that in the experimental group I, similar movements of the paralyzed arm were promoted due to the double-task exercise on the unstable surface, and sensory feedback and posture strategies were well utilized in the legs, which was found to be effective in reducing the GS and improving the balance ability. Therefore, based on these results, the double-task exercise on an unstable surface should be fully utilized for the rapid rehabilitation of stroke patients.

Studies on sterile filters in the preparation of N-13 ammonia injection (N-13 암모니아 주사액 제조 시 멸균필터의 흡착율 차이에 관한 비교 평가)

  • Oh, Chang Bum;Kim, Si Hwal;Cha, Min Jung;Shin, Jin;Ji, Yong Gi;Choi, Sung Ook
    • The Korean Journal of Nuclear Medicine Technology
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    • v.23 no.1
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    • pp.64-68
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    • 2019
  • Purpose In the preparation process for N-13 Ammonia injections, there were radioactive medicines adsorbed on filters remarkably. Hereby, we have compared the adsorption rate and quality test on Millex GS filter and Satorious Minisart filter, both representatively hydrophilic sterilizing filters, also evaluated which filter is more accommodative for N-13 Ammonia injection. Materials and Methods The filters used for sterilization of N-13 Ammonia injections were Millex GS filter($0.22{\mu}m$) mand Satorious Minisart filter ($0.2{\mu}m$), which are generally used to strain aqueous solutions. After the N-13 Ammonia passes through each sterilization filter, the adsorption rate of the filter (n=10) is determined by measuring not only the radioactivity through the filter also the amount of radioactivity remaining in it using a Dose Calibrator. The N-13 Ammonia injections after each filter is tested by the quality control test to conform to the Samsung Medical Center standard. Results The ratio of radioactivity passed through Millex GS indicated $29.0{\pm}17.6%$. Satorious Minisart filters output was $80.9{\pm}3.2%$, respectively. Each ratio of radioactivity adsorbed on the sterile filter was $71.0{\pm}17.6%$ for Millex GS and $19.1{\pm}3.2%$ for the Satorious Minisart filters, respectively. Furthermore, on the ratio of filtered radioactivity, Using Satorious Minisart filter showed about 2.8 times higher than using Millex GS filter. The quality testing of N-13 Ammonia injections through each filter met the Samsung Medical Center standard. Conclusion The Millex GS filter is composed of cellulose acetate and cellulose nitrate, whereas the Satorious Minisart filter if composed only of cellulose acetate. Therefore, the presence of cellulose nitrate in the membrane seems to have made differences. Therefore, the use of Satorious Minisart filter in the preparation of N-13 Ammonia injection solution minimized the loss of radioactive medicines due to filter adsorption, thereby improving the synthesis yield.

Purification and Characterization of Xylanase from Bacillus sp. GS (Bacillus sp. GS가 생산하는 Xylanase의 정제 및 특성)

  • An, Jun-Bae;Park, Heon-Guk;Lee, Gye-Ho
    • The Korean Journal of Food And Nutrition
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    • v.7 no.1
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    • pp.16-22
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    • 1994
  • Xylanase from Bacillus sp. GS was purified through acetone precipitation, DEAE-Sephadex A-50 ion exchange chromatography and Sephadex G-100 gel filtration. The optimum reaction temperature of purified xylanase was 50t . Its optimum pH was between pH 6.0 and pH 6.5. This enzyme was stable below 5$0^{\circ}C$ for several hours and stable at between pH 5.5 and pH 8.0. The enzyme activity of xylanase was remarkably increased by Co++ and Cu++ ions. According to the study of hydrolysis mode of this enzyme, it was turned out to be ends type xylanase that can produce xylooligosaccharides, known as bifidogenic factor, from xylan.

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Comparative Analysis of Image Fusion Methods According to Spectral Responses of High-Resolution Optical Sensors (고해상 광학센서의 스펙트럼 응답에 따른 영상융합 기법 비교분석)

  • Lee, Ha-Seong;Oh, Kwan-Young;Jung, Hyung-Sup
    • Korean Journal of Remote Sensing
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    • v.30 no.2
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    • pp.227-239
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    • 2014
  • This study aims to evaluate performance of various image fusion methods based on the spectral responses of high-resolution optical satellite sensors such as KOMPSAT-2, QuickBird and WorldView-2. The image fusion methods used in this study are GIHS, GIHSA, GS1 and AIHS. A quality evaluation of each image fusion method was performed with both quantitative and visual analysis. The quantitative analysis was carried out using spectral angle mapper index (SAM), relative global dimensional error (spectral ERGAS) and image quality index (Q4). The results indicates that the GIHSA method is slightly better than other methods for KOMPSAT-2 images. On the other hand, the GS1 method is suitable for Quickbird and WorldView-2 images.

The characteristic of photosynthetic pigments distribution of the sediment in the shellfish farm (패류 양식장 퇴적물의 광합성색소 분포 특성)

  • Kim, Sook-Yang;Choi, Minkyu;Hwang, Dong-Woon;Lee, In-Seok
    • The Korean Journal of Malacology
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    • v.29 no.4
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    • pp.291-301
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    • 2013
  • This study researched and compared the sedimentation environment and photosynthetic pigments at Gomso (GS) -bay, Yoja (YJ) -bay and KangJin (KJ) -bay in May, 2012. It was shown that KJ-bay consist of C (clay) and M (mud), and GS-bay consists of Zs (silty sand) and Sz (sandy silt). Averagely, IL of YJ-bay was 4.98%, KJ-bay 6.10%, and GS-bay 1.45%. As for COD concentration, there were no places that exceeded Japanese sediment contamination standard 20 mg/g-dry. As for AVS concentration, in case of KJ-bay, two places exceeded Japanese sediment contamination standard 0.2 mg/g-dry. If we look into the average C/N ratio of bays, YJ-bay showed 8.50, KJ-bay 6.60, and GS-bay 5.52, thus all of them showed the characteristic of oceanic origin. As for the plankton make-up classified by photosynthetic pigments, diatom was dominant, and both ratios of pigment and C/chlorophyll. a showed the relatively lower distribution at GS-bay than at KJ-bay and YJ-bay. It is judged that this is related to the characteristic of flow-in, and it implies that predation process and decomposition by organic matters actively occurs at YJ-bay and KJ-bay.

Isolation and Evaluation of Bacillus Strains for Industrial Production of 2,3-Butanediol

  • Song, Chan Woo;Rathnasingh, Chelladurai;Park, Jong Myoung;Lee, Julia;Song, Hyohak
    • Journal of Microbiology and Biotechnology
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    • v.28 no.3
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    • pp.409-417
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    • 2018
  • Biologically produced 2,3-butanediol (2,3-BDO) has diverse industrial applications. In this study, schematic isolation and screening procedures were designed to obtain generally regarded as safe (GRAS) and efficient 2,3-BDO producers. Over 4,000 candidate strains were isolated by pretreatment and enrichment, and the isolated Bacillus strains were further screened by morphological, biochemical, and genomic analyses. The screened strains were then used to test the utilization of the most common carbon (glucose, xylose, fructose, sucrose) and nitrogen (yeast extract, corn steep liquor) sources for the economical production of 2,3-BDO. Two-stage fed-batch fermentation was finally carried out to enhance 2,3-BDO production. In consequence, a newly isolated Bacillus licheniformis GSC3102 strain produced 92.0 g/l of total 2,3-BDO with an overall productivity and yield of 1.40 g/l/h and 0.423 g/g glucose, respectively, using a cheap and abundant nitrogen source. These results strongly suggest that B. licheniformis, which is found widely in nature, can be used as a host strain for the industrial fermentative production of 2,3-BDO.

Effect of Gleditsiae Spina extract on immune cells and cytokines in OVA-induced asthmatic mice (급각자가 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향)

  • Song, Sang-Jin;Park, Yang-Chun
    • The Journal of Internal Korean Medicine
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    • v.26 no.1
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    • pp.143-155
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    • 2005
  • Objective : The purpose of this research is to examine the effects of Gleditsiae Spina (GS) extract on immune cells and cytokines in ovalbumin (OVA)-induced asthmatic mice. Methods : In vivo, C57BL/6 mice were sensitized and handicapped by OVA for 12 weeks. During this experiment, the one group was then treated with GS extract for the later 8 weeks (3 times per week) and analyzed by ELISA, flow cytometer and RT-PCR. Results : In vivo, there were significant decreases in eosinophils, IL-4, IL-5, IL-13, IgE in BALF (bronchoalveolar lavage fluid). However, $IFN-{\gamma}$ in BALF of GS group increased significantly, compared with that of control group. Additionally, the population of $CD3e^-/CCR3^+,\;CD69^+/CD3e^+,\;IgE^+/B220^+,\;CD11b^+/Gr-l^+$ cells in the GS group decreased. Conclusion : The results of this study support a role for GS as an effective treatment for asthma in its experimental success in significantly decreasing inflammation and asthma reactions, and in increasing $IFN-{\gamma}$, which helps prevent such reactions.

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The Synergic Anti-inflammatory Impact of Gleditsia sinensis Lam. and Lactobacillus brevis KY21 on Intestinal Epithelial Cells in a DSS-induced Colitis Model

  • Kim, Younghoon;Koh, Ji Hoon;Ahn, Young Jun;Oh, Sejong;Kim, Sea Hun
    • Food Science of Animal Resources
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    • v.35 no.5
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    • pp.604-610
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    • 2015
  • We investigated the synergic anti-inflammatory activity of Gleditsia sinensis Lam. (GS) extract and Lactobacillus brevis KY21 both in vitro and in vivo. Western blot analysis and immunostaining showed that AKT phosphorylation that increased by the exposure of LPS were significantly decreased by the presence of either GS extract or L. brevis KY21. In addition, p65 intracellular transport was critically inhibited by GS extract and L. brevis KY21. We further studied these effects using an in vivo dextran sulfate sodium (DSS)-induced mouse model. Body weight, food intake, and clinical scores were dramatically decreased after treatment with DSS, whereas these effects were palliated by the addition of GS extract and L. brevis KY21. Importantly, transcription of genes encoding pro-inflammatory cytokines including IL-1β, TNF-α, and IFN-γ in mesenteric lymph nodes (MLN) and the spleen were increased by DSS treatment, whereas they were inhibited by the presence of GS extract and L. brevis KY21.

The Prediction Model Development for Water Supply Monitoring System based on Machine Learning (머신러닝을 고려한 상수도 모니터링 시스템 예측 모델 개발)

  • Shim, Kyu Dae;Choung, Joon Yeon;Kim, Chang Ryong;Kim, Dong Kyun
    • Proceedings of the Korea Water Resources Association Conference
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    • 2022.05a
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    • pp.395-395
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    • 2022
  • 본 연구는 머신러닝 기반의 상수도 모니터링 시스템의 예측 모델을 개발하고, 예측 모델의 적용이 가능성을 검토하였다. 상수도모니터링 시스템은 상수관망에 설치된 센서에서 수집된 자료를 모니터링 할 수 있어 운영자의 상수도 시설물의 관리 편의성을 높일 수 있다. 특히 수리학적 모델을 적용하여 계산된 값과 측정된 값을 비교해 이상치가 발생하면 운영자에게 이를 알려주므로 시스템내의 문제점을 빠르게 확인할 수 있다. 그러나 수리학적 모델은 입력자료가 증가됨에 따라 계산시간이 많이 소요되는 문제가 있고, 계산된 값의 정확도가 낮아지므로. 이러한 문제를 보완하기 위해 머신러닝 기반의 예측 모델을 개발하여 이를 해결하고자 하였다. 예측 모델은 GS 이니마 브라질(GS Inima Brazil)에서 운영중인 아라사투바(Aracatuba) 지역 주사라(Jussara) DMA(District Metered Area)의 2018년 1월에서 7월까지의 운영자료를 이용하였으며, 상수도 모니터링 시스템에서 상수관로 수압에 영향을 미치는 영향 인자들을 분석하고, 하이퍼파라미터 최적화를 통한 수압 예측 모델을 개선하였다. 금회 연구는 머신러닝 기반의 모델을 통하여 상수관망의 시간변화에 따른 장래 예측 수압을 검토할 수 있었다는데 큰 의의가 있다.

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