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http://dx.doi.org/10.5851/kosfa.2015.35.5.604

The Synergic Anti-inflammatory Impact of Gleditsia sinensis Lam. and Lactobacillus brevis KY21 on Intestinal Epithelial Cells in a DSS-induced Colitis Model  

Kim, Younghoon (BK21 Plus Graduate Program, Department of Animal Science and Institute Agricultural Science & Technology, Chonbuk National University)
Koh, Ji Hoon (Division of Food Bioscience and Technology, Korea University)
Ahn, Young Jun (Division of Food Bioscience and Technology, Korea University)
Oh, Sejong (Division of Animal Science, Chonnam National University)
Kim, Sea Hun (Division of Food Bioscience and Technology, Korea University)
Publication Information
Food Science of Animal Resources / v.35, no.5, 2015 , pp. 604-610 More about this Journal
Abstract
We investigated the synergic anti-inflammatory activity of Gleditsia sinensis Lam. (GS) extract and Lactobacillus brevis KY21 both in vitro and in vivo. Western blot analysis and immunostaining showed that AKT phosphorylation that increased by the exposure of LPS were significantly decreased by the presence of either GS extract or L. brevis KY21. In addition, p65 intracellular transport was critically inhibited by GS extract and L. brevis KY21. We further studied these effects using an in vivo dextran sulfate sodium (DSS)-induced mouse model. Body weight, food intake, and clinical scores were dramatically decreased after treatment with DSS, whereas these effects were palliated by the addition of GS extract and L. brevis KY21. Importantly, transcription of genes encoding pro-inflammatory cytokines including IL-1β, TNF-α, and IFN-γ in mesenteric lymph nodes (MLN) and the spleen were increased by DSS treatment, whereas they were inhibited by the presence of GS extract and L. brevis KY21.
Keywords
anti-inflammatory activity; herbal extract; probiotic bacteria; DSS;
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1 Bamias, G., Corridoni, D., Pizarro, T. T., and Cominelli, F. (2012) New insights into the dichotomous role of innate cytokines in gut homeostasis and inflammation. Cytokine 59, 451-459.   DOI
2 Bedaiwi, M. K. and Inman, R. D. (2014) Microbiome and probiotics: Link to arthritis. Curr. Opin. Rheumatol. 26, 410-415.   DOI
3 Howarth, G. S., Xian, C. J., and Read, L. C. (1998) Insulin-like growth factor-I partially attenuates colonic damage in rats with experimental colitis induced by oral dextran sulphate sodium. Scand. J. Gastroenterol. 33, 180-190.   DOI
4 Jamaluddin, M., Casola, A., Garofalo, R. P., Han, Y., Elliott, T., Ogra, P. L., and Brasier, A. R. (1998) The major component of IκBα proteolysis occurs independently of the proteasome pathway in respiratory syncytial virus-infected pulmonary epithelial cells. J. Virol. 72, 4849-4857.
5 Abreu-Martin, M. T., Vidrich, A., Lynch, D. H., and Targan, S. R. (1995) Divergent induction of apoptosis and IL-8 secretion in HT-29 cells in response to TNF-alpha and ligation of Fas antigen. J. Immunol. 155, 4147-4154.
6 Baggiolini, M., Walz, A., and Kunkel, S. L. (1989) Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J. Clin. Invest. 84, 1045-1049.   DOI
7 Christensen, H., Bisgaard, M., Angen, O., and Olsen, J. E. (2002) Final classification of Bisgaard taxon 9 as Actinobacillus arthritidis sp. nov. and recognition of a novel genomo-species for equine strains of Actinobacillus lignieresii. Int. J. Syst. Evol. Microbiol. 52, 1239-1246.
8 Osawa, Y., Nagaki, M., Banno, Y., Brenner, D. A., Asano, T., Nozawa, Y., Moriwaki, H., and Nakashima, S. (2002) Tumor necrosis factor alpha-induced interleukin-8 production via NF-kappaB and phosphatidylinositol 3-kinase/Akt pathways inhibits cell apoptosis in human hepatocytes. Infect. Immun. 70, 6294-6301.   DOI
9 Podolsky, D. K. (2005) Inflammatory bowel disease. N. Engl. J. Med. 358, 1965-1968.
10 Chow, L. M., Chui, C. H., Tang, J. C., Teo, I. T., Lau, F. Y., Cheng, G. Y., Wong, R. S., Leung, T. W., Lai, K. B., Yau, M. Y., Gou, D., and Chan, A. S. (2003) Gleditsia sinensis fruit extract is a potential chemotherapeutic agent in chronic and acute myelogenous leukemia. Oncol. Rep. 10, 1601-1607.
11 Okayasu, I., Hatakeyama, S., Yamada, M., Ohkusa, T., Inagaki, Y., and Nakaya, R. (1990) A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterol. 98, 694-702.   DOI
12 Dieleman, L. A., Palmen, M. J., Akol, H., Bloemena, E., Peña, A. S., Meuwissen, S. G., and Van Rees, E. P. (1998) Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines. Clin. Exp. Immunol. 114, 385-391.   DOI
13 FAO/WHO (2002) Joint FAO/WHO Working Group Report on Drafting Guidelines for the evaluation of Probiotics in Food.
14 Fujimori, S., Tatsuguchi, A., and Gudis, K. (2007) High dose probiotic and prebiotic cotherapy for remission induction of active Crohn's disease. J. Gastroenterol. Hepatol. 22, 1199-1204.   DOI
15 Lee, Y. S., Han, O. K., Park, C. W., Yang, C. H., Jeon, T. W., Yoo, W. K., Kim, S. H., and Kim, H. J. (2005) Pro-inflammatory cytokine gene expression and nitric oxide regulation of aqueous extracted Astragali radix in RAW 264.7 macrophage cells. J. Ethnopharmacol. 100, 289-294.   DOI
16 Li, W. H., Zhang, X. M., Tian, R. R., Zheng, Y. T., Zhao, W. M., and Qiu, M. H. (2007) A new anti-HIV lupane acid from Gleditsia sinensis Lam. J. Asian Nat. Prod. Res. 9, 551-555.   DOI
17 Madsen, K. A. C., Soper, P., McKaigney, C. H. J., Yachimec, C., Doyle, J., Jewell, L., and De Simone, C. (2001) Probiotic bacteria enhance murine and human intestinal epithelial barrier function. Gastroenterol. 121, 580-591.   DOI
18 Mantovani, A., Bussolino, F., and Dejana, E. (1992) Cytokine regulation of endothelial cell function. FASEB J. 6, 2591-2599.   DOI
19 Rumi, G., Tsubouchi, R., Okayama, M., Kato, S., Mózsik, G., and Takeuchi, K. (2004) Protective effect of lactulose on dextran sulfate sodium-induced colonic inflammation in rats. Dig. Dis. Sci. 49, 1466-1472.   DOI
20 Mizoguchi, A. and Mizoguchi, E. (2008) Inflammatory bowel disease, past, present and future: Lessons from animal models. J. Gastroenterol. 43, 1-17.   DOI
21 Takahashi, A., Wada, A., Ogushi, K., Maeda, K., Kawahara, T., Mawatari, K., Kurazono, H., Moss, J., Hirayama, T., and Nakaya, Y. (2001) Production of beta-defensin-2 by human colonic epithelial cells induced by Salmonella enteritidis flagella filament structural protein. FEBS Lett. 508, 484-488.   DOI
22 Weber, C. K., Liptay, S., Wirth, T., Adler, G., and Schmid, R. M. (2000) Suppression of NF-kB activity by sulfasalazine is mediated by direct inhibition of IkB kinases alpha and beta. Gastroenterol. 119, 1209-1218.   DOI
23 Yi, J. M., Kim, J., Park, J. S., Lee, J., Lee, Y. J., Hong, J. T., Bang, O. S., and Kim, N. S. (2015) In vivo anti-tumor effects of the ethanol extract of Gleditsia sinensis thorns and its active constituent, cytochalasin H. Biol. Pharm. Bull. 38, 909-912.   DOI