• Journal of Microbiology and Biotechnology
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• v.28 no.3
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• pp.409-417
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• 2018

Biologically produced 2,3-butanediol (2,3-BDO) has diverse industrial applications. In this study, schematic isolation and screening procedures were designed to obtain generally regarded as safe (GRAS) and efficient 2,3-BDO producers. Over 4,000 candidate strains were isolated by pretreatment and enrichment, and the isolated Bacillus strains were further screened by morphological, biochemical, and genomic analyses. The screened strains were then used to test the utilization of the most common carbon (glucose, xylose, fructose, sucrose) and nitrogen (yeast extract, corn steep liquor) sources for the economical production of 2,3-BDO. Two-stage fed-batch fermentation was finally carried out to enhance 2,3-BDO production. In consequence, a newly isolated Bacillus licheniformis GSC3102 strain produced 92.0 g/l of total 2,3-BDO with an overall productivity and yield of 1.40 g/l/h and 0.423 g/g glucose, respectively, using a cheap and abundant nitrogen source. These results strongly suggest that B. licheniformis, which is found widely in nature, can be used as a host strain for the industrial fermentative production of 2,3-BDO.

• Journal of Pharmaceutical Investigation
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• v.35 no.2
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• pp.75-80
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• 2005

A promising application of Lactococcus lactis is its use as live vehicles for production and delivery of heterologous proteins of vaccines and therapeutic substances. Because L. lactis has GRAS ('generally regarded as safe') status, we tested whether L. lactis could function as the carrier of the Ll protein of human papillomavirus (HPV) type 16. The RNA level expression of Ll gene was detected in L. Lactis. The Ll protein was expressed in L. lactis with Ll gene. The growth of strains L. lactis with an empty plasmid (pAMJ328) and L. lactis with Ll-encoding plasmid (pAMJ328-Ll) was slightly decreased in comparison with the growth of strains L. lactis (wild type). However, all the three strains of L. lactis maintained the ability to ferment sugars primarily into lactic acid, indicating that Ll protein did not affect the biochemical property of L. lactis. These results suggest that L. lactis, capable of carrying Ll protein, might be further developed as a biocompatible oral protein delivery system.

• Proceedings of the Korea Water Resources Association Conference
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• 2008.05a
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• pp.254-258
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• 2008

본 연구는 격자기반 운동파 강우유출모형 KIMSTORM(grid-based KIneMatic wave STOrm Runoff Model)의 기능을 개선하고 적용성을 평가하는 것이다. KIMSTORM은 김성준(1998)이 개발한 분포형 강우유출모형으로 포화상태의 지표흐름 및 토양수분상태의 시공간적인 분포를 파악할 수 있다. UNIX C++ 언어로 개발되었으며, GRASS 형태의 ASCII Grid를 입출력하도록 구성되어 있는 모형으로 UNIX 운영체제에서 구동이 가능하다. 그러나 UNIX와 GRASS는 최근에 많이 이용되지 않는 추세로 KIMSTORM 모형을 이용한 홍수유출해석이 적극적으로 활용되는데 주요 제약사항이 되어 왔다. 본 연구에서는 KIMSTORM을 윈도우즈 환경에서 운영될 수 있도록 FORTRAN 90을 이용하여 재개발하였으며 주요개선 사항으로, ESRI ASCII Grid 형태의 GIS(geographic information system) 자료 입력, 물리적 침투모의 방법인 GAML (Green-Ampt and Mein- Larson) 적용, 공간강우 입력가능, 정렬 알고리즘을 이용한 계산속도의 개선, 모형 자료입력 등 전처리 기능개선, 계산결과의 자동평가 및 분포도출력 등 후처리 방식개선으로 요약할 수 있다. 개선된 모형 GAML에 의한 침투방법을 적용하여, 남강댐유역($2,293\;km^2$)의 6개 강우사상을 대상으로 결정계수, Nash & Sutcliffe 모형효율계수, 용적편차, 첨두유량의 상대오차, 첨두시간의 절대오차를 이용하여 적용성을 평가하였으며, 민감도분석결과 초기토양수분조건과 하천조도계수가 가장 큰 민감도를 나타내었다.

• Korean journal of applied entomology
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• v.28 no.1
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• pp.10-15
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• 1989

This studies were carried out to selected high pathogenic nuclear polyhedrosis viruses(NPVs) against Pseudaletia(=Leucania) separata for the introduction of microbiol control of the insect NPV in Korea. Among 21 NPVs, Sesamia inferens and 4 P. separata NPV strains were highly pathogenic against P. separata when fed orchard grass leaves smeared virus suspension on the 2nd instar larvae. Three NPV strains (LsNPV-F, LsNPV-G, LsNPV-Y) were more susceptible to the younger instar than the older instar P. separata larvae when fed artificial diet mixed with the virus to the insect.

• Spatial Information Research
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• v.7 no.1
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• pp.39-48
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• 1999

A GRId-based Soil MOsture Routing Model(GRISMORM) which predicts temporal variation and spatial distribution of water balance on a daily time step for each grid element of the watershed was developed. The model was programmed by C-language which aims for high flexibility to any kind of GIS softwares. The model uses ASCII-formatted map data supported by the irregular gridded map of the GRASS(Geographic Resources Analysis Support System)-GIS and generates daily or monthly spatial distribution map of water balance components within the watershed. The model was applied to Ipyunggyo watershed(75.6$km^2$) ; the part of Bocheongchun watershed. Predicted streamflows resulting from two years(95 and 96) daily data were compared with those observed at the watershed outlet. The results of temporal variation and spatial distribution of soil moisture are also presented by using GRASS.

• Journal of Nutrition and Health
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• v.40 no.sup
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• pp.50-65
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• 2007

감미료는 감각을 만족시키는 기능을 가지며, 에너지를 공급하는 영양적 감미료와 에너지를 공급하지 않는 비 영양적 감미료로 분류 될 수 있다. 영양적 감미료들은(설탕, 과당 등) 미국 식약청에서는 일반적으로 안전하다고 인식(generally recognized as safe, GRAS) 되고 있다. 그러나 감미료 섭취는 적절한 영양섭취와 건강과 관련하여 아직 많은 우려를 가지고 있다. 첨가당으로부터의 에너지 섭취가 총 에너지 섭취의 25% 이상이면 식사의 질(quality)이 낮아진다. 지난 수 십 년 간, 당 섭취가 행동적인 면에 부정적으로 작용한다는 많은 이론들이 대두되었다. 그러나 포도당은 뇌의 주요 에너지원이다. 포도당의 기억력 향상기능은 모든 연령에서 보고되었으며, 특히 젊은 층보다는 노인에서 더 효과적이었다. 특히 적당량으로 섭취하였을 때 포도당의 섭취는 단어기억, 이야기 기억, 24시간 전에 학습한 정보에 대한 기억, 경계(vigilance)의 향상, 반응시간의 단축, 정신적 계산 능력의 향상, 시뮬레이터 조절에서의 실수감소, 스투르프효과(Stroop effect)의 감소, 지능검사의 향상, 실제사건에 대한 기억력 검사와 웩슬러 척도(Wechsler Scale)에서의 향상과 같은 여러 인지능력 검사에서 좋은 영향을 미친다고 한다. 포도당 용액과 간식의 섭취는 기억력을 개선한다. 아침식사를 거르는 것은 아동들에서 낮은 학습능력을 보인다. 인지능력 변화와 관련 있는 식이는 뇌에 포도당을 공급하는 것과 관련 있다. 과당도 동물실험에서 기억력을 향상시키는 기능이 있다는 것을 보여주고 있다. 본 논문은 당 섭취가 인지능력에 미치는 영향에 대한 과학적인 연구들을 종합하여 정리한 것이다.

• Korean journal of applied entomology
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• v.9 no.1
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• pp.49-51
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• 1970

1. Claviceps species or Graminaceae were investigated from Kyonggi-Do Korea including Kwang-neung, Anseung, Pyongtack, Chingpyong Yichun and Suwon from June to November, 1969. 2. Sclerotia of Claviceps species were collected from 16 species of Graminaceae consisted of 11 genera. 3. New hosts of Claviceps species including 15 species in 10 genera were reported in Korea, 4. Claviceps pucilla Ces. on Themeda riandra FORSK. var. japonica M. was recorded in addition to Claviceps purpurea (Fr.) Tul. 5. Claviceps Purpurea (FR.) Tul. on Miscanthus Sinensis ANDERS occured at 449m above sea level near Suwon.

• Bulletin of the Korean Chemical Society
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• v.35 no.10
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• pp.3071-3076
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• 2014

Proteomic analyses of the excretory/secretory proteins from the RH strain of Toxoplasma gondii have been performed to understand their functions in the host-parasite interaction. A total of 34 proteins were identified from LC/MS/MS analysis and their abundance was estimated by spectral counting methods. Among them, 8 species of micronemal proteins (MICs), 2 species of rhoptry proteins (ROPs), and 6 species of dense granular proteins (GRAs) were confirmed. Besides these, 18 species of protein were newly identified, and their cellular functions were estimated from sequence analysis. The three most abundant of the 34 identified extractor/secretory proteins-GRA1, GRA7 and GRA2-were confirmed to be highly expressed in T. gondii using the spectral count method. This phenomenon is another demonstration of the importance of GRA proteins for the penetration and survival of T. gondii.

• Water for future
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• v.29 no.4
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• pp.199-208
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• 1996

The multiple flow direction algorithm to calculate the spatial variation of the saturation tendency, i.e. topographic index, is integrated into the Geogrphic Information System, GRASS. A procedure is suggested to consider the effect of a tile system on calculating the topographic index. A small agricultural subwatershed (3.4$\textrm{km}^2$) is used for this study. The impact of a tile system on the groundwater table can be effectively considered by the Laplace's equation to the DEM. The analysis shows that a tile system has a high degree of saturation compared to the case without tile drainage, and the predicted riparian area is well fitted to the actual watershed condition. A procedure is suggested to consider the effect of tile system on calculating the topographic index.

• Proceedings of the Korea Water Resources Association Conference
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• 1997.05a
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• pp.192-198
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• 1997

The spatial variation of saturation tendency can be calculated from the Digital Elevation Model (DEM) employing the multiple flow direction algorithm on the platform of Geographic Resources Support Analysis System (GRASS). It is expected that a bettter understanding of dynamical runoff processes in hillslope hydrological scale is obtained through tracing various runoff path such as infiltration excess overland flow component, strutation excess overland flow component and subsurface runoff component. A procedure is suggested to consider the effect of a tile system on calculating the topographic index. A small agricultural subwatershed (3.4 km2) is used for this study.