Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
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Lactic Acid Bacteria as Oral Antigen Protein Carriers

유산균을 이용한 겸구용 항원 단백질 수송능 연구

  • Published : 2005.04.20
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Abstract

A promising application of Lactococcus lactis is its use as live vehicles for production and delivery of heterologous proteins of vaccines and therapeutic substances. Because L. lactis has GRAS ('generally regarded as safe') status, we tested whether L. lactis could function as the carrier of the Ll protein of human papillomavirus (HPV) type 16. The RNA level expression of Ll gene was detected in L. Lactis. The Ll protein was expressed in L. lactis with Ll gene. The growth of strains L. lactis with an empty plasmid (pAMJ328) and L. lactis with Ll-encoding plasmid (pAMJ328-Ll) was slightly decreased in comparison with the growth of strains L. lactis (wild type). However, all the three strains of L. lactis maintained the ability to ferment sugars primarily into lactic acid, indicating that Ll protein did not affect the biochemical property of L. lactis. These results suggest that L. lactis, capable of carrying Ll protein, might be further developed as a biocompatible oral protein delivery system.

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References

  1. Willem M de Vos, Gene expression systems for lactic acid bacteria, Curr. Opin. Microbiol., 2, 289-295 (1999) https://doi.org/10.1016/S1369-5274(99)80050-2
  2. S. Nouaille, L. A. Ribeiro, A. Miyoshi, D. Pontes, Y. Le Loir, S.C. Oliveira, P. Langella and V. Azevedo, Heterologuous protein production and delivery systems for Lactococcus lactis, Genet. Mol. Res., 2, 102-11 (2003)
  3. Y. Le Loir, A. Gruss, S. D. Ehrlich and P. Langella, A nine-residue synthetic propeptide enhances secretion efficiency of heterologous proteins in Lactococcus lactis, J. Bacteriol., 180, 1895-1903 (1998)
  4. R. Kirnbauer, F. Booy, N. Cheng, D. R. Lowy and J. T. Schiller, Papillomavirus L1 major capsid protein selfassembles into virus-like particles that are highly immunogenic, Proc. Natl. Acad. Sci. USA, 89, 12180-12184 (1992)
  5. J. A. Suzich, S. J. Ghim, F. J. Palmer-Hill, W I. White, J. K. Tamura, J. A. Bell, J. A. Newsome, A. B. Jenson and R. Schlegel, Systemic immunization with papillomavirus L1 protein completely prevents the development of viral mucosal papillomas, Proc. Natl. Acad. Sci. USA, 92, 11553-11557 (1995)
  6. D. Nardelli-Haefliger, R. B. Roden, J. Benyacoub, R. Sahli, J. P. Kraehenbuhl, J. T. Schiller, P. Lachat, A. Potts and P. De Grandi, Human papillomavirus type 16 virus-like particles expressed in attenuated Salmonella typhimurium elicit mucosal and systemic neutralizing antibodies in mice, Infect. Immun., 8, 3328-3336 (1997)
  7. S. Biemelt, U. Sonnewald, P. Galmbacher, L. Willmitzer and M. Muller, Production of human papillomavirus type 16 virus-like particles in transgenic plants, J. Virol., 77, 9211-9220 (2003) https://doi.org/10.1128/JVI.77.17.9211-9220.2003
  8. Y. K. Oh, T. Sohn, J. S. Park, M. J. Kang, H. G. Choi, J. A. Kim, W. K. Kim, J. J. Ko and C. K. Kim, Enhanced mucosal and systemic immunogenicity of human papillomavirus-like particles encapsidating interleukin-2 gene adjuvant, Virology, 328, 266-273 (2004) https://doi.org/10.1016/j.virol.2004.06.047
  9. S. Even, N. D. Lindley and M. Cocaign-Bousquet, Molecular physiology of sugar catabolism in Lactococcus lactis IL1403, J. Bacteriol., 183, 3817-3824 (2001) https://doi.org/10.1128/JB.183.13.3817-3824.2001
  10. S. M. Madsen, J. Arnau, A. Vrang, M. Givskov and H. Israelsen, Molecular characterization of the pH-inducible and growth phase-dependent promoter P170 of Lactococcus lactis, Mol. Microbiol., 32, 75-87 (1999) https://doi.org/10.1046/j.1365-2958.1999.01326.x