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http://dx.doi.org/10.5012/bkcs.2014.35.10.3071

Comprehensive Proteome Analysis of the Excretory/Secretory Proteins of Toxoplasma gondii  

Lee, Won-Kyu (Department of Chemistry, College of Natural Sciences, Kookmin University)
Ahn, Hye-Jin (Department of Parasitology and the Catholic Institute of Parasitic Diseases, College of Medicine, Catholic University of Korea)
Baek, Je-Hyun (Center of Biomedical Mass Spectrometry (CBMS), Diatech Korea Co., Ltd.)
Lee, Chong-Heon (Department of Oral Pathology, College of Dentistry, Dankook University)
Yu, Yeon Gyu (Department of Chemistry, College of Natural Sciences, Kookmin University)
Nam, Ho-Woo (Department of Parasitology and the Catholic Institute of Parasitic Diseases, College of Medicine, Catholic University of Korea)
Publication Information
Bulletin of the Korean Chemical Society / v.35, no.10, 2014 , pp. 3071-3076 More about this Journal
Abstract
Proteomic analyses of the excretory/secretory proteins from the RH strain of Toxoplasma gondii have been performed to understand their functions in the host-parasite interaction. A total of 34 proteins were identified from LC/MS/MS analysis and their abundance was estimated by spectral counting methods. Among them, 8 species of micronemal proteins (MICs), 2 species of rhoptry proteins (ROPs), and 6 species of dense granular proteins (GRAs) were confirmed. Besides these, 18 species of protein were newly identified, and their cellular functions were estimated from sequence analysis. The three most abundant of the 34 identified extractor/secretory proteins-GRA1, GRA7 and GRA2-were confirmed to be highly expressed in T. gondii using the spectral count method. This phenomenon is another demonstration of the importance of GRA proteins for the penetration and survival of T. gondii.
Keywords
Excretory/secretory protein; Toxoplasma gondii; Micronemal protein; Rhoptry protein; Dense granule protein;
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