• Applied Biological Chemistry
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• 제34권4호
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• pp.318-326
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• 1991

사염화탄소(carbon tetrachloride, 이하 $CCl_4$라함)에 의한 간 중독시 flavonoids가 미치는 영향을 보기 위하여 $CCl_4$를 투여한 뒤 rutin, hesperidin을 주사하여 경시적으로 간 microsome과 혈청중 total cholesterol, transaminase(GOT, GPT) 및 alkaline phosphatase(Alk. P) 활성도를 측정 비교하여 다음과 같은 결론을 얻었다. (1) 간장중 total cholesterol 함량은 rutin과 hesperidin 1회 투여후 1일에 대조군과 비교할 때 감소하다가 2일에 45%까지 증가되었으나 4일 후에는 정상으로 완전히 회복 되었다. 이와 같은 현상은 혈청중 cholesterol 함량에도 직접적으로 영향을 주어 시간이 경과함에 따라 어느정도 저하됨을 알 수 있었지만 혈청중에서 positive한 결과를 얻기는 어려웠다. (2) 간의 microsome 분획의 Alk. P 활성도는 대조군의 활성치에 비하여 2일에 급격히 증가를 보인 후 4일만에 대조군보다 낮은 활성을 보이다가 6일까지 완만하게 증가하였다. 그러나 본 연구에서 Alk. P 활성도는 $CCl_4$ 투여군과 비교할 때 flavonoids 투여후 별다른 변동은 없었다. 그러나 혈청중 Alk. P 활성도는 hesperidin 투여로 인하여 저하 효과를 나타냈다. (3) GPT 활성도를 측정한 결과 간의 microsome 분획에서는 hesperidin 2회 투여에서, 그리고 혈청중 GPT 활성은 rutin과 hesperidin 두군 모두에서 아주 낮은 활성을 보여 flavonoids가 $CCl_4$ 투여로 인한 간독성을 해독하는 작용이 있음을 보여 주었다. (4) $CCl_4$독성을 일으킨 흰쥐의 간 microsome내 GOT 활성은 rutin 투여로 인하여 불규칙한 변동을 보여 rutin이 GOT활성에 별다른 영향을 미치지 않는 것으로 나타났다. 또한 hesperidin 투여군에서는 증감의 변동없이 대조군치와 거의 비슷한 수준을 유지하였지만 $CCl_4$ 투여군과 비교할 때 낮은 활성을 보였다. 혈청중에서 GOT 활성은 flavonoids 1회 투여군에서는 4일만에, 2회 투여군에서는 6일만에 서서히 영향을 미침을 알 수 있었다. 이상과 같이 $CCl_4$에 의하여 상승되는 total cholesterol, GPT, GOT, Alk. P의 활성도는 flavonoids중 rutin과 hesperidin 성분이 감소시키는 작용이 있는 것으로 보이며, 특히 hesperidin 성분은 $CCl_4$ 중독을 억제 또는 방어하고 치료에 효과가 있는 것으로 본다.

• Journal of Nutrition and Health
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• 제7권2호
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• pp.37-51
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• 1974

This Study was carried out to observe the effect of nutritional condition on the change of protein metabolism in the animal body by feeding on imbalanced protein diet. A total 242 growing male albino rats, weighing $115{\sim}120$ gm, were used for the experimental animals. The rats were fed on the standard diet(st), protein flee diet(pf) and imbalanced protein diet(ib) for twelve weeks respectively. Hemoglobin, packed cell volume in blood, and total nitrogen, amino acid nitrogen, urea-nitrogen, creatinine, transaminases(GPT, GOT) in liver and serum, and total nitrogen in small intestine, and total nitrogen, urea-nitrogen In small intestine, and total nitrogen, urea-nitrogen, creatinine, urea-nitrogen/creatinine ratio in urine were measured. The results obtained are as follows; 1. The gained body weight were lower in pf group and ib group than those of st group. The gained body weight fed for 12 weeks, were 80% lower in pf group than those of st group, and the body weight of pf group for $50{\sim}75$ days feeding were $40{\sim}60%$ decreased, compared with the stating weight, and then all of them died. 2. The change of the brain, liver, kidney, spleen and small intestine by feeding on imbalanced diet for 12 weeks were no remarkable difference with the starting weight, but those of protein free diet group were half or more decrease and those were significantly lower in spleen and small intestine especially than the other organ 3. The contents of hemoglobin in pf group for 8 weeks feeding, and the packed cell volume in pf group for 8 weeks feeding and in ib group for 12 weeks feeding were decreased. but those of the other feeding group were almost same value. 4. The total nitrogen in the liver, small intestine and serum of each diet group were no remarkable difference respectively. The contents of amino acid nitrogen in pf group for 2 and 6 weeks feeding were increased. 5. On transaminases: a) The cycle of increase and decrease of GPT activities were come periodically and the interval of cycle were fast in the early stage of feeding and slow there-after. b) The GPT activities were decreased gradually in pf group after feeding and those were increased in ib group for 6 weeks feeding but decreased there-after. The frequency of cycle were more GPT than GOT and specially those of GPT in early stage of feeding were two or three times while GOT was one. c) The interval of increase and decrease in GOT and amino acid nitrogen cycle were similar tendency. 6. The contents of total nitrogen, creatinine and urea-nitrogen of pf group in urine were decreased very sharply from sharting feeding to one week but increased dully from six weeks to eight weeks feeding. The contents of urea-nitrogen of ib group were increased dully by feeding on ten weeks but decreased by feeding on twelve weeks. From the above results, it is concluded that the trend of the metabolic change is maintained equally by homeostatic mechanism using the endogenous protein source during a certain period by imbalanced protein diet feeding. The homeostatic mechanism is come peridically, very fast in early stage of feeing and than slow there-after.

• 한국식품영양과학회지
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• 제25권5호
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• pp.839-845
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• 1996

대추 메탄을 추출물이 사염화탄소에 의한 휜쥐의 간손상에 미치는 영향을 알아보기 위하여 대조군, 대추메탄올 추출물 투여군, 사염화탄소 투여군 및 대추메탄올 추출물을 투여한 후 사염화탄소를 투여한 군으로 나눠, 2, 4주간 사육하여 혈청 중의 transaminase (GOT 및 GPT), 간 중의 lipid peroxide량, glutathione 함량, xanthine oxidase, SOD, catalase, GSH-Px 등의 활성도의 변화를 관찰하였다. 사염화탄소(50%, 1.0ml/kg) 투여군의 혈청 중 GOT및 GPT의 활성도, 간중TBA 반응성 산물량은 2, 4주에서 대조군에 비하여 현저히 증가되었으나, 대추 메탄올 추출물을 투여한 후 사염화탄소를 투여할 때 GOT의 경우 2, 4주에서, 간 중 TBA 반응성 산물량은 4주에서 사염화탄소 투여군에 비하여 유의성있는 감소효과를 보였다. 유리기 생성계 효소인 xanthine oxidase 활성도에서, 사염화탄소 투여군은 대조군에 비하여 현저한 증가를 보였으나, 대추 메탄올 추출물을 투여한 후 사염화탄소를 투여한 군에서는 감소되었다. 유리기 해독계 효소인 SOD, catalase 및 GSH-Px의 활성도도 사염화탄소 투여로 모두 증가되었으나, 대추 메탄올 추출물을 투여한 후 사염화탄소 투여로 SOD는 4주에서, GSH-Px는 2, 4주에서 대조군에 비하여 유의한 감소효과를 나타냈으며, catalase는 투여기간에 따라 감소는 되었으나 유의성은 보이지 않았다. 이상의 실험결과에서, 사염화탄소 투여로 혈청 및 간 중의 각종 효소 활성도 및 지질과산화물량이 증가되었는데 이는 사염화탄소가 간 중의 효소계 및 간세포의 microsome을 심하게 손상시켰음을 알 수 있으며, 대추 메탄올 추출물을 전처리하므로써 사염화탄소에 의하여 현저히 증가된 각종 효소활성도 및 지질과 산화물량이 감소되었는데, 이는 대추 메탄올 추출물이 간세포의 괴사와 효소의 유출을 저 해하고 간의 저항력 및 간기능을 유지시키므로써 간 보호작용을 할 수 있을 것으로 사료된다.

• Journal of Nutrition and Health
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• 제33권4호
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• pp.386-394
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• 2000

This study was conducted to investigate the effects of shellfish hydrolysate on lipid metabolism in rats fed high fat diet. Male Sprague-Dawley rate weighting approximately 110g were fed basal control diet, high fat diet and high fat diet plus 4 different shellfish hydrolyates for 4 weeks. The shellfish hydrolysates from the different sources, were oyster, hard-shelled mussel, little neck clam and march clam. After 4 weeks, serum GOT, GPT, ${\gamma}$-GTP, triglyceride and total cholesterol was significantly decreased in shellfish hydrolysates supplementation with high fat diet compared to basal control and high-fat group(p<0.05). The total lipid and cholesterol content in liver showed significant decrease(p, 0.05). There were no different in serum GPT, HDL-cholesterol, liver total cholesterol and lipid of rats between basal control diet and high-fat diet. The unsaturated fatty acids, specific components of shellfishes were a little components in shellfish hydrolysate as they were a low and not different among the groups and were most well reflected in liver and plasma. Considering digestive and absorptive process of in human body, it was assumed that the hypolipidemic effect of shellfish was not under the influence of unsaturated fatty acids but the other components, peptides, taurine and betaine and so on was detected in the process of hyperlipidemia induced by high-fat diet.

• 한국식생활문화학회지
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• 제5권2호
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• pp.253-257
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• 1990

The effect of water extract in fruits of Omija (Schizandra chinensis Ballion) on liver function in expermental liver injuries induced by $CCl_4$. The levels of GPT, GOT activities in serum were decreased apparently on the dosage of water extract in fruits of Omija correspond to raw Omija 1g compared to control group. The levels of Microsomal protein, glycogen, and pyruvate in liver and of Urea nitrogen, GPT, GOT, LDH in serum showed a trend toward restoration of normalization. No effective of levels of hepatic G6P DH activity and serum free fatty acid by water extract in fruits of Omija.

• 한국식품위생안전성학회지
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• 제2권4호
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• pp.201-208
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• 1987

The effects of ginseng on the blood biochemical components of heavy metals poisoning in mice were examined and following results were obtained. Albumin was decreased slightly in the heavy metal administration groups. But Serum Calcium, Glucose were almost the same as that of control group during the period. Heavy metal poisoning mice showed low levels of serum Total protein and A/G ratio. They also showed high levels of serum GOT, GPT, BUN, Cholesterol, Creatinine and Triglycerides. However, the administration of ginseng significantly inhibited the elevation of them.

• 대한약침학회지
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• 제8권3호
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• pp.21-29
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• 2005

In order to Experimental study effects of ${\ulcorner}Triglii\;Semen{\lrcorner}$ on the blood in Rats, we have made rats by administering Animal control chamber for two weeks, then have administered. The results is were summerized as follows: 1. The total cholesterol, triglyceride and Hct, Hb by generally rats oral ingestion by Triglii semen of the sample group were decreased as compared with those of the control group. 2. GOT, GPT by generally rats oral ingestion were increased as compared those of control group. According to the above results, Triglii Semen showed inhibition on decrease of serum total cholesterol and Hb, Hct, WBC, RBC contents as well as inhibition on increase of GOT, GPT.

• 한국임상수의학회지
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• 제17권1호
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• pp.28-31
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• 2000

An animall health monitoring system in Gyeongnam area(near Chinju) was studied to analysis of biochemical risk factors in 617 herds. Clinical serum factors such as glutamate oxaloacetate transaminase(GOT), glutamate pyruvate transaminase(GPT), Ca, P, Mg, glucose, and cholesterol were measured with automatic biochemical analyzer(Ra-X7T Techmmicon, USA). In serum analysis, 613 cattle were within normal llimits(GOT: 9.5-85 IU-dl, GPT: 25-77IU/dl, total protein: 5.8-8.5g/dl, Ca: 4.2-12.4mg/dl, P: 4.6-9.7mg/dl, Mg: 1.5-3.0mg/dl, glucose: 48-120mg/dl, Cholesterol: 70-170mg/dl), the other cattle showed high glucose and high cholesterol level. It is proposed that clinical serum factors to be estimated may be valuable for developing of animal health monitoring system model.

• 대한한방소아과학회지
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• 제21권2호
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• pp.89-96
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• 2007

Objectives The purpose of this study is to examine of the effect of Sachunghwan on the liver disease. Methods It was induced liver damage rats by thioacetamide and dosed the extract orally and measured the activity rate of GOT, GPT, ALP and LDH. Results 1. GOT activity was more decreased in the experimental goup than in the control goup. Group I showed continuous significance after 72 hour, Group II was significance after 96 hours. 2. GPT activity was more decreased in the experimental group than in the control group. Group I was effective after 72 hours, but group II was effective after 96 hours. 3. The significance of ALP activity in the Group I and group II was revealed after 72 hours. 4. The significance of LDH activity in the Group I and group II was revealed after 96 hours. Conclusions It was showded that Sachunghwan extract was effective on liver disease.

• 한국동물위생학회지
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• 제15권2호
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• pp.128-133
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• 1992

To obtain the basic data for blood chemistry, the effects of the time elapse of serum separation on bovine blood chemical values were investigated. The results obtained are summerized as follows : 1. The constant fluctuation tendency and significance was not detected in GOT, GPT, BUN, Creatinine, ALP, CPX, Cholesterol, Ca, Mg and Pi. 2. Glucose showed the decrease tendency according to the time elapse of serum separation. As the values at 12 and 24 hours showed significant lower values than those at 1 and 2 hours (p＜0.01) it was thougth that separation time of serum should be focussed for the glucose determination.