• Asian-Australasian Journal of Animal Sciences
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• 제19권6호
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• pp.784-788
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• 2006

Microsatellite polymorphism and the genetic relationship were estimated using genotype information of 305 horses from 11 microsatellite loci. The breeds include the indigenous Korean breeds, Korean native horse (102) and Jeju racing horse (56) together with Japan Hokkaido horse (5), Mongolian horse (19), Thoroughbred horse (108), Quarter horse (11) and Przewalskii horse (4). Allelic frequencies, the number of alleles per locus were estimated by direct counting from observed genotype, and genetic variability was computed using the CERVUX software and DISPAN. The number of alleles per locus varied from 6 (HMS6) to 18 (ASB17) with an average value of 10.45 in horse breeds. The expected total heterozygosity ($H_T$) and coefficient of gene differentiation ($G_{ST}$) ranged 0.764-0.921 (the average value was 0.830) and 0.102-0.266 (the average value was 0.180) in horse breeds, respectively. Four populations (Przewalskii horse, Japan Hokkaido horse, Quarter horse, Thoroughbred horse) showed lower heterozygosity than the average value (the average value was 0.710). The expected heterozygosity within breed ($H_S$) and mean no. of observed alleles ranged from $0.636{\pm}0.064$ (Japan Hokkaido horse) to $0.809{\pm}0.019$ (Mongolian horse), and from 2.73 (Przewalskii horse) to 8.27 (Korean native horse), respectively. The polymorphic information content (PIC) ranged from 0.490 (Przewalskii horse) to 0.761 (Mongolian horse) with an average value of 0.637 in horse breeds. The results showed three distinct clusters with high bootstrap support: the Korean native horse cluster (Korean native horse, Mongolian horse), the European cluster (Przewalskii horse, Thoroughbred horse), and other horse cluster (Jeju racing horse, Japan Hokkaido horse, and Quarter horse). A relatively high bootstrap value was observed for the Korean native horse cluster and European cluster (87%), and the Korean native horse and Mongolian horse (82%). Microsatellite polymorphism data were shown to be useful for estimating the genetic relationship between Korean native horse and other horse breeds, and also be applied for parentage testing in those horse breeds.

• Weed & Turfgrass Science
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• 제4권2호
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• pp.135-143
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• 2015

Typhula ishikariensis Imai is a causal agent of Typhula snow mold, one of the most important turfgrass diseases in northern regions of the United States. Within Wisconsin isolates, there are three district groups clustered with known isolates of T. ishikariensis var. ishikariensis, var. canadensis and var. idahoensis as identified by RAPD markers. To further investigate the genetic relationship among these groups (varieties), monokaryon-monokaryon and dikaryon-monokaryon mating experiments were conducted. Mating types from var. ishikariensis, var. canadensis and var. idahoensis isolates were paired in all possible combinations. Pairings between var. canadensis and var. idahoensis were highly compatible, while no compatibility was detected between var. ishikariensis and either var. canadensis or var. idahoensis. These results indicate that var. ishikariensis is genetically separated from var. canadensis and var. idahoensis, whereas var. canadensis and var. idahoensis appeared to be genetically related to each other as a taxonomic unit. In the genetic relationship with the known biological species, var. ishikariensis and var. canadensis were genetically related to biological species I and II, respectively. However, var. idahoensis was not compatible with any of the biological species, suggesting that the pathogen may be in the process of biological speciation from var. canadensis.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.32-32
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• 2019

Mutation breeding is the useful tool to improve agronomic traits in various crop species. Soybean is most important crop and is rich in protein and oil contents. Despite of the importance as economic value and various genetic resource of soybean, there have been limited studies of genetic relationship among mutant resources through radiation breeding. In this study, the agronomical phenotype for selecting various genetic resources was evaluated in 528 soybean mutant lines. As a result, 210 soybean mutants with their original cultivars were selected with various traits. We named 210 selected lines as Mutant Diversity Pool (MDP). The genetic diversity and the relationship of the MDP were investigated using TRAP and TE-TRAP markers. In TRAP analysis, sixteen primer combination (PC)s were used and a total of 551 fragments were amplified. The highest (84.00%) and the lowest (32.35%) polymorphism levels were showed in PC MIR157B+Ga5 and B14G14B+Ga3, respectively. The mean of PIC values was 0.15 ranging from 0.07 in B14G14B+Sa12 to 0.23 in MIR157B+Sa4. Phylogenetic and population structure analysis indicated that the 210 MDP lines dispersed to four groups among the wild types and their mutants. The highest genetic diversity among populations was observed between lines Paldal and 523-7 (Fst=0.409), whereas the lowest genetic diversity was between population KAS360-22 and 94seori (Fst=0.065). AMOVA showed 11.583 (21.0%) and 43.532 (79.0%) variations in inter and intra mutant population, respectively. Overall, the genetic similarity of each intra mutant populations was closer than that of inter mutant population. A total of 408 fragments were amplified in the 210 MDP using twelve PCs of TE-TRAP markers that were obtained from a combination of three TIR sequence of transposable elements (MITE-stowaway; M-s, MITE-tourist; M-t, PONG). The highest (77.42%) and the lowest (56.00%) polymorphism levels were showed in PONG+Sa4 and PONG+Sa12, respectively. The mean of PIC values was 0.15 ranging from 0.09 in M-s+Sa4 and M-s+Ga5 to 0.21 in M-t+Ga5. AMOVA of M-s showed 2.209 (20%) and 8.957 (80%) variations in inter and intra mutant population, respectively. AMOVA of M-t showed 2.766 (18%) and 12.385 (82%) variations in inter and intra mutant population, respectively. AMOVA of PONG showed 3.151 (29%) and 7.646 (71%) variations in inter and intra mutant population, respectively. According to our study, the PONG had higher inter mutant population and lower intra mutant population. This mean was that for aspect of radiation sensitivity, M-s and M-t showed higher mobility than that of PONG. Our results suggest that the TRAP and the TE-TRAP markers may be useful for assessing the genetic diversity and relationship among soybean MDP and help to improve our knowledge of soybean mutation/radiation breeding.

• 한국수산과학회지
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• 제42권2호
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• pp.146-150
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• 2009

Genetic variation and relationship of two wild (Pampus argenteus and P. echinogaster) and one cultured (P. chinesis) pomfret fish belonging to the genus Pampus were assessed. Specimens were collected from Korea and China and subjected to amplified fragment length polymorphism (AFLP) DNA fingerprinting. Four primer combinations generated a total of 304 DNA fragments ranging from 153 to 251 bands. Polymorphism and genetic diversity of cultured P. chinensis (22.9% and 0.038) were significantly lower than the two wild species of P. argenteus (93.6% and 0.311) and P. echinogaster (94.0% and 0.290). Genetic distance ranged from 0.335 (P. argenteus and P. echinogaster) to 0.646 (P. argenteus and P. chinensis) and showed a congeneric relationship within this genus. Twenty one of specific AFLP markers from four primer combinations bands were produced. These results suggest that AFLP polymorphism may be a useful marker for genetic identification among the three species studies here.

• BMB Reports
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• 제43권12호
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• pp.836-841
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• 2010

In the era of personal genomics, predicting the individual response to drug-treatment is a challenge of biomedical research. The aim of this study was to validate whether interaction information between genetic and transcriptional signatures are promising features to predict a drug response. Because drug resistance/susceptibilities result from the complex associations of genetic and transcriptional activities, we predicted the inter-relationships between genetic and transcriptional signatures. With this concept, captured genetic polymorphisms and transcriptional profiles were prepared in cancer samples. By splitting ninety-nine samples into a trial set (n = 30) and a test set (n = 69), the outperformance of relationship-focused model (0.84 of area under the curve in trial set, P = $2.90{\times}10^{-4}$) was presented in the trial set and validated in the test set, respectively. The prediction results of modeling show that considering the relationships between genetic and transcriptional features is an effective approach to determine outcome predictions of drug-treatment.

• 한국자원식물학회지
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• 제25권3호
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• pp.329-338
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• 2012

Genus Taraxacum has been widely used as a folkloric medicine for treatment of diverse diseases. The genetic diversity and relationship among 32 accessions belonging to five Taraxacum species (T. mongolicum T. coreanum, T. coreanum var. flavescens, T. officinale and T. laevigatum) which collected from field, mountain, island and seaside of Korea were evaluated using ISSR markers. A total of 142 ISSR loci detected in the overall species were all polymorphic loci (100%) and interspecies polymorphisms obtained from Korean native and naturalized species were 98.2% and 94.5%, respectively. The genetic similarity matrix (GSM) among 32 accessions ranged from 0.025 to 0.860 with an average of 0.303. According to the clustering analysis, the Korean native species and naturalized species were divided two major clusters. In addition, the different species were divided into independent groups except for the T. coreanum and T. coreanum var. flavescens, and all the 32 accessions could be classified into 7 categories. The study findings indicate that Taraxacum accessions have a high genetic diversity and the dandelion accessions as breeding materials can be effectively utilized for the improvement of Taraxacum breeding.

• 동의생리병리학회지
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• 제18권6호
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• pp.1699-1709
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• 2004

Genetic variations and relationship based on the sequences of cpDNA trnL-F gene, nrDNA ITS and ETS region among the twenty four taxa including Panax ginseng C.A. Meyer and its related species were investigated. And taxonomic status and molecular phylogenetic relationship between P. ginseng and related groups were discussed. Molecular systematic data from cpDNA and nrDNA sequences were very useful to elucidate the genetic variations and relationships among the treated taxa. It was found that P. ginseng is the independent unique species with distinct genetic limitation from the related species such as P. quinquefolius, P. japonicum, P. notoginseng and P. pseudoginseng. P. ginseng including cultivated types as well as wild ones formed monophyletic group with high genetic similarities. P. quinquefolius and P. japonicum were the most related sister groups of P. ginseng based on the molecular phylogenetic results in this study.

• 농업과학연구
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• 제42권3호
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• pp.245-251
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• 2015

Records of 490,767 cows collected from 1990 to 2012 by dairy herd milk test of National Agriculture Cooperative Federation The pedigree of dairy cattle were provided by Korea Animal Improvement Association. The data were used to analyze the longevity of dairy cows with the life traits such as days in milk, number of lactation, productive life, and life span. The data were also used to investigate genetic relationship of these longevity traits with profitability of dairy cows, including heritability and genetic correlation. The profitability was calculated with simulation of milk income and production costs for individual cows. Days in milk among the traits had -0.287, -0.572 and -0.536 of genetic correlation with number of lactations, productive life and lifespan, respectively. The heritabilities of life span, number of lactations, productive life, and days in milk were found to be 0.045, 0.047, 0.059 and 0.081, respectively. Genetic correlations of profit with productive life, number of lactations, and days in milk were identified as 0.072, 0.080, 0.098 and 0.101. These results suggested that days in milk was most desirable traits to represent longevity of Holstein dairy cattle. In general, since longevity and profitability were close genetic relationship each other, genetic improvement of longevity is necessary for better profitable cows.

• 한국균학회지
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• 제37권1호
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• pp.28-32
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• 2009

본 연구의 목적은 식용 및 약용버섯인 아위(阿魏)버섯(Pleurotus ferulae) 균주와 느타리버섯속(Pleurotus spp.) 종간의 유전적 유연관계를 이해하는 것이었다. RAPD 분석결과 이들 버섯 종들은 유사도 80% 수준에서 7개의 그룹으로 분류되었다. 노랑느타리(P. cornucopiae), 사철 2호(P. florida), 여름 2호(P. sajor-caju)는 각각의 그룹으로 분류 되었고, 큰느타리 균주인 26060과 P. fuscus var. ferulae 균주인 26065은 같은 그룹으로 분류되었다. 4개의 느타리 균주는 흑평을 제외한 수한 1호, 원형 1호, 춘추 1호가 한그룹을 형성하였고, 아위버섯 균주 중 일본설이를 제외한 박달, 아위, 천산 1호, 아위예산 균주가 하나의 그룹을 형성하였다. 아위버섯 균주인 일본설이는 느타리 균주인 흑평과 같은 그룹에 속하였다.

• 한국자원식물학회지
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• 제13권2호
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• pp.104-110
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• 2000

가시오갈피 및 오갈피의 수집종 간의 유연관계를 구명하기 위하여 RAPD 분석을 한 결과 10개의 primer를 선발하였으며 G+C의 수가 모두 60%이상이었다. 10개의 primer를 사용하여 얻을 수 있는 총 밴드 수는 106개 였으며 이중 monomorphic한 밴드는 17.9%에 해당하는 19개였으며 나머지 87개는 polymorphic한 것으로 나타났다. 10개의 primer를 사용하여 얻은 106개의 밴드를 각각 하나의 형질(character)로 보아 이를 유연관계를 분석한 결과 영월 수집종과 일본종 및 지리산 오갈피와 서울오갈피를 포함하는 군(Group I )과 국내종과 러시아종, 중국종을 포함하는 군(Group II)으로 나뉘어졌으며 genetic distance값의 평균은 0.61이었다. Group I으로 분류된 북해도 가시오갈피는 국내의 각 수집지역의 가시오갈피나 러시아 가시오갈피와 원연의 관계인 것으로 나타났으며 2군에 포함된 수집 지역종 간의 원연 관계 중 춘천 수집종은 국내의 다른 지역인 잠곡이나 태기산 오대산 등과 비교하여 러시아 산이나 중국산에 대하여 더 근연의 관계를 나타내었다.