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Genetic Variation of the Three Pampus spp. (Pisces: Stromateidae) using Amplified Fragment Length Polymorphism (AFLP)

AFLP 분석에 의한 병어속 (Pampus) 3종의 유전 변이

  • Yoon, Young-Eun (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Park, Sang-Yong (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Bae, Joo-Seung (Incheon Fisheries Office, Incheon Metropolitan City) ;
  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
  • 윤영은 (순천향대학교 해양생명공학과) ;
  • 박상용 (순천향대학교 해양생명공학과) ;
  • 배주승 (인천광역시청 수산사무소) ;
  • 방인철 (순천향대학교 해양생명공학과)
  • Received : 2009.01.29
  • Accepted : 2009.04.01
  • Published : 2009.04.30

Abstract

Genetic variation and relationship of two wild (Pampus argenteus and P. echinogaster) and one cultured (P. chinesis) pomfret fish belonging to the genus Pampus were assessed. Specimens were collected from Korea and China and subjected to amplified fragment length polymorphism (AFLP) DNA fingerprinting. Four primer combinations generated a total of 304 DNA fragments ranging from 153 to 251 bands. Polymorphism and genetic diversity of cultured P. chinensis (22.9% and 0.038) were significantly lower than the two wild species of P. argenteus (93.6% and 0.311) and P. echinogaster (94.0% and 0.290). Genetic distance ranged from 0.335 (P. argenteus and P. echinogaster) to 0.646 (P. argenteus and P. chinensis) and showed a congeneric relationship within this genus. Twenty one of specific AFLP markers from four primer combinations bands were produced. These results suggest that AFLP polymorphism may be a useful marker for genetic identification among the three species studies here.

Keywords

References

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