• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2002년도 추계학술대회
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• pp.135-139
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• 2002

Yeast cells respond to condition of amino acid starvation by synthesizing GCN4, a typical eukaryotic transcriptional activator, which regulates the expression of many amino acids biosynthetic genes. By introducing point mutations in the DNA binding domain of GCN4, mutants with normal DNA binding activity but defective in transcriptional activity were isolated to identify unknown proteins that could suppress the mutant phenotype under an amino acid depletion condition. As a result, SSB(Stress-Seventy B) subfamily proteins were identified as suppressors of mutant GCN4. SSB proteins were known as a member of yeast hsp70 family that probably aids passage of nascent chain through ribosomes. Among them, the mechanism of suppression by SSB2 on the defective GCN4 mutant strains is under investigation. Gcn4p directly interacts with Ssb2p through the basic DNA binding domain of GCN4. It suggests the possibility that physical interaction might induce the transcriptional activation of Gcn4p.

• 한국미생물·생명공학회지
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• 제19권1호
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• pp.37-44
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• 1991

진핵세포 유전자의 기초대사발현의 조절계를 밝히기 위한 일환으로, 효모의 histidine생합성계 효소의 구조유전자 HIS5를 이용하였다. HIS5 유전자는 충분한 아미노산 조건하에서는 발현이 억제되어 비교적 높은 기초발현만을 하나, 어떤 아미노산이 결핍되면 탈억제되어 높은 발현량을 보이며 탈억제는 cis의 작용인자인 promoter상의 5'-TGACTC-3' 및 trans 작용인자 GCN4와 GCD17 GCN2등이 관여한다. trans 작용인자들에 의한 HIS5 유전자의 발현량의 변화를 간단하게 측정하기 위하여, HIS5 promoter와 repressible acid phoshates(APase)의 구조유전자중 promoter를 제거한 DNA단편을 연결시켜 HIS5-PHO5 융합유전자를 이용하였다. gcn2 및 gcn4 변이주의 APase 활성은 야생주와 비교하여 3내지 4배 낮았으며, gcn2변이주와 gcn2 gcn4 이중변이주의 APase 활성은 유사하였다.

• 대한의생명과학회지
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• 제19권3호
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• pp.266-269
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• 2013

Hox genes encode transcription factors important for anterior-posterior body patterning at early stages of embryonic development. However, the precise mechanisms by which signal pathways are stimulated to regulate Hox gene expression are not clear. In the previous study, protein kinase B alpha (Akt1) has been identified as a putative upstream regulator of Hox genes, and Akt1 has shown to regulate Gcn5, a prototypical histone acetyltransferase (HAT), in a negative way in mouse embryonic fibroblast (MEF) cells. Since the activity of HAT such as the CBP/p300, and PCAF (a Gcn5 homolog), was down-regulated by Akt through a phosphorylation at the Akt consensus substrate motif (RXRXXS/T), the amino acid sequence of Gcn5 protein was analyzed. Mouse Gcn5 contains an Akt consensus substrate motif as RQRSQS sequence while human Gcn5 does not have it. In order to see whether Akt1 directly binds to Gcn5, immunoprecipitation with anti-Akt1 antibody was carried out in wild-type (WT) mouse embryonic fibroblast (MEF) cells, and then western blot analysis was performed with anti-Akt1 and anti-Gcn5 antibodies. Gcn5 protein was detected in the Akt1 immunoprecipitated samples of MEFs. This result demonstrates that Akt1 directly binds to Gcn5, which might have contributed the down regulation of the 5' Hoxc gene expressions in wild type MEF cells.

• Molecules and Cells
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• 제24권2호
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• pp.194-199
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• 2007

GCN4 is a typical eukaryotic transcriptional activator that is implicated in the expression of many genes involved in amino acids and purine biosyntheses under stress conditions. It is degraded by 26S proteasomes following ubiquitination. However, the immediate receptor for ubiquitinated Gcn4p has not yet been identified. We investigated whether ubiquitinated Gcn4p binds directly to Rpn10p as the ubiquitinated substrate receptor of the 26S proteasome. We found that the level of Gcn4p increased in cells deleted for Rpn10p but not in cells deleted for RAD23 and DSK2, the other ubiquitinated substrate receptors and, unlike Rpn10p, neither of these proteins recognized ubiquitinated Gcn4p. These results suggest that Rpn10p is the receptor that binds the polyubiquitin chain during ubiquitin-dependent proteolysis of Gcn4p.

• 한국전자거래학회지
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• 제23권1호
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• pp.37-45
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• 2018

현재 프로그램이 운용되는 시스템은 기존의 싱글코어 및 멀티코어 환경을 넘어서 매니코어, 부가 프로세스 및 이기종 환경까지 그 영역이 확장되고 있는 중이다. 하지만, 기존 연구의 경우 NVIDIA 벤더에서 나온 아키텍쳐 및 CUDA로의 병렬화가 주로 이루어졌고 AMD에서 나온 범용 GPU 아키텍쳐인 GCN 아키텍쳐에 대한 성능향상에 관한 연구는 제한적으로 이루어졌다. 이런 점을 고려해 본 논문에서는 GCN 아키텍쳐의 GPGPU 환경인 OpenCL 내에서의 성능향상 기법에 대해 연구하고 실질적인 성능향상을 보였다. 구체적으로, 행렬 곱셈과 컨볼루션을 적용한 GPGPU 프로그램을 본 논문에서 제시한 성능향상 기법을 통해 최대 30% 이상의 실행시간을 감소시켰으며, 커널 이용률 또한 40% 이상 높였다.

• 대한의생명과학회지
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• 제18권2호
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• pp.165-168
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• 2012

Hox proteins containing DNA-binding homedomain act as transcription factors important for anteroposterior body patterning during vertebrate embryogenesis. However, the precise mechanisms by which signal pathways are transduced to regulate the Hox gene expression are not clear. In the course of an attempt to isolate an upstream regulatory factor(s) controlling Hox genes, protein kinase B alpha (Akt1) has been identified as a putative regulator of Hox genes through in silico analysis (GEO profile). In the Gene Expression Omnibus (GEO) dataset GDS1784 at the NCBI (National Center for Biotechnology Information) site, Hox genes were differentially expressed depending on the presence or absence of Akt1. Since it was not well known how Akt1 regulates the specific Hox genes, whose transcription was reported to be regulated by epigenetic modifications such as histone acetylation, methylation etc., the expression of Gcn5, a histone acetyltransferase (HAT), was analyzed in wild type (WT) as well as in $Akt1^{-/-}$ mouse embryonic fibroblast (MEF) cells. RT-PCR analysis revealed that the amount of Gcn5 mRNA was similar in both WT and $Akt1^{-/-}$ MEFs. However, the protein level of Gcn5 was significantly increased in $Akt1^{-/-}$ MEF cells. The half life of Gcn5 was 1 hour in wild type whereas 8 hours in $Akt1^{-/-}$ MEF. These data all together, indicate that Gcn5 is post-transcriptionally down-regulated and the protein stability is negatively regulated by Akt1 in MEF cells.

• Journal of Microbiology
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• 제37권3호
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• pp.154-158
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• 1999

The arginine residue at position 243 (Arg 243) of the yeast transcription factor, Gcn4p, is invariably conserved among bZIP transcription factors. Using site-directed oligonucleotide saturation mutagenesis involving two-step polymerase chain reaction (PCR) amplification, random mutations were successfully introduced at the codon of 243 in the basic domain of Gcn4p. This mutant library was transformed ito Gcn4p defective yeast strain and selected for the transcriptionally active colonies. All colonies which were transcriptionally active had arginines in the codon 243. In this study, the strand preference by Taq polymerase during mutagenesis was also tested. Oligonucleotides were specially designed to test whether or not the polymerase was preferred using the strand as a template. A population of randomly mutated products were cloned into an appropriate vector and characterized by DNA sequencing analysis. Saturation mutagenesis which was performed efficiently by this method revealed a strong bias in terms of strand preference of Taq polymerase by an approximate ratio of 3 to 1 in this study.

• 한국융합학회논문지
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• 제11권6호
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• pp.23-28
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• 2020

인터넷상의 소통을 위해 댓글 시스템은 필수적이다. 하지만 온라인상의 익명성을 악용하여 타인에 대한 부적절한 표현 등의 악성 댓글 또한 존재한다. 악성 댓글로부터 사용자를 보호하기 위해 악성/정상 댓글의 분류가 필요하고 이는 텍스트 분류로 구현할 수 있다. 자연어 처리에서 텍스트 분류는 중요한 주제 중 하나이고 최근 BERT 등 pretrained model을 활용한 연구와 GCN, GAT 등의 그래프 구조를 활용한 연구가 활발히 진행되고 있다. 본 연구에서는 실제 공개된 댓글에 대해 BERT, GCN, GAT 을 활용하여 댓글 분류 시스템을 구현하고 성능을 비교하였다. 본 연구에서는 그래프 기반 모델을 사용한 시스템이 BERT 대비 높은 성능을 보여주었다.

• Journal of Microbiology and Biotechnology
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• 제9권1호
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• pp.122-125
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• 1999

Gcn4p, a transcriptional activator protein of the yeast, Sacchromyces cerevisiae, binds to the specific sequence in the promoters of many amino acid biosynthetic genes for general control. The serine residue (Ser 242) of Gcn4p directly contacts the DNA. Here, for inspecting the DNA binding properties and the level of transcriptional activation of Gcn4p, we introduced a polymerase chain reaction (PCR) site-directed saturation mutation library into the Ser 242 site using 2 outside primers and 2 oligonucleotides with its codons fully degenerated. The sequencing analysis of 146 samples revealed the even nucleotide distribution within the experimental error showing 23, 26, 25, and 26％ frequency of U, C, A, and G bases, respectively. This method turned out to be a simple, fast, and economical method for constructing a library of all 20 amino acids at specific codon.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2023년도 추계학술발표대회
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• pp.652-655
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• 2023

우리나라에서는 지난 10년간 매년 4만 건 내외의 화재가 발생하여 많은 인명 피해와 경제적 손실이 발생하고 있다. 화재가 발생했을 때는 화재를 신속히 진압하여 인명 피해와 경제적 손실을 최소화하여야 한다. 또한, 화재 사고를 예방하기 위해 화재의 발화 원인이 무엇인지 알아내야 한다. 기존의 화재 경보 시스템에서는 온도, 연기, 불꽃 센서 등으로 화재를 감지하였으나 오경보나 화재를 인식하지 못하는 문제, 화재 원인을 구분하지 못하는 문제 등이 있었다. 또한, 사람이 화재 발생을 인지하기까지 시간이 많이 소요될 수 있고 부재로 인해 화재 상황인식이 늦어질 수도 있는 문제가 있었다. 이러한 문제를 해결하기 위해 본 논문에서는 GCN(Graph Convolutional Network) 모델을 이용하여 화재 상황에서의 복합 센서 상황을 학습해서 실제 화재 사고가 발생했을 때 화재의 원인을 구분할 수 있는 모델을 제안한다.