• Title/Summary/Keyword: GC-1

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Identification of Volatile Flavor Components of Chinese chive and Baek-Seok Chie (중국 부추와 백석 부추의 휘발성 풍미 성분의 동정)

  • 이혜정;박희옥
    • The Korean Journal of Food And Nutrition
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    • v.10 no.4
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    • pp.539-543
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    • 1997
  • This study was carried out to investigate the Chinese and Baek-Seok chive. We collect the volatile components of Chinese and Baek-Seok chive by dynamic head space method. Chinese chive was analyzed by Gas chromatography-Mass Spectrometry(GC-MS). 28 components including 20 sulfides, 5 alcohols, 1 benzene and 2 aldehydes compounds were identified in samples, Also Baek-Seok chive was analyzed by GC-MS. 32 components including 19 sulfides, 10 alcohols, 2 benzonoides and 1 aldehyde compounds were confirmed.

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Electrochemical Determination of Dopamine Based on Carbon Nanotube-Sol-Gel Titania-Nafion Composite Film Modified Electrode

  • Park, Ji-Ae;Kim, Byung-Kun;Choi, Han-Nim;Lee, Won-Yong
    • Bulletin of the Korean Chemical Society
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    • v.31 no.11
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    • pp.3123-3127
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    • 2010
  • A highly sensitive electrochemical detection method for dopamine (DA) has been developed by relying on a multiwalled carbon nanotube (CNT)-sol-gel titania-Nafion composite film modified glassy carbon (GC) electrode. The CNT-titania-Nafion/GC electrode exhibited excellent electrocatalytic activity towards DA. Therefore, the CNT-titania-Nafion/GC electrode showed improved voltammetric and amperometric responses for DA compared to those obtained with both titania-Nafion/GC and Nafion/GC electrodes. The CNT-titania-Nafion/GC electrode gave a linear response ($R^2$ = 0.999) for DA from $0.5\;{\mu}M$ to 0.5 mM with a detection limit (S/N = 3) of $0.1\;{\mu}M$ and a good sensitivity of 150 mA/M while other electrodes such as CNT-Nafion/GC, titania-Nafion/GC, and a bare GC gave a sensitivity of 89, 39, and 36 mA/M, respectively. Besides, the CNT-titania-Nafion/GC electrode displayed very fast response time within 2 s. The modified electrode showed good selectivity against ascorbic acid. The modified electrode showed good stability and reproducibility. The CNT-titania-Nafion/GC electrode was applied to the determination of DA in urine and serum samples.

A Study of Globular Cluster Systems in the Coma, Fornax, and Virgo Clusters of Galaxies from HST ACS and WFC3/IR Imaging

  • Cho, Hyejeon
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.1
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    • pp.29.1-29.1
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    • 2017
  • I present new near-infrared (NIR) photometry of globular cluster (GC) systems associated to a cD galaxy NGC 4874 in the core of the Coma cluster and 16 early-type galaxies in the Fornax and Virgo clusters of galaxies using the Infrared Channel of the Wide Field Camera 3 (WFC3/IR) on board the Hubble Space Telescope (HST). Combining these high-resolution NIR data with new HST Advanced Camera for Surveys (ACS) optical photometry for NGC 4874 and existing ACS GC catalogs from the ACS Fornax and Virgo Cluster Surveys, I have examined for the first time the GC systems in a statistically significant optical/NIR sample of galaxies spanning a wide range of luminosities and colors. A primary goal of this study is to explore empirically whether the distributions of purely optical and hybrid optical - NIR color indices for extragalactic GCs have different forms and whether the relations between these color indices are nonlinear, indicating that they behave differently with underlying metallicity. I find that some GC systems of large galaxies in our sample show color bimodalities that differ between the optical and optical - NIR colors, in the sense that they have disparate ratios of "blue" and "red" peak GCs, as well as differing ratios in their color dispersions. Consistent with these results, I find empirically that the dependence of hybrid optical-NIR color on purely optical color is nonlinear, with an inflection at intermediate metallicities. These findings underscore the importance of understanding the nature of galaxy-to-galaxy variations in the GC color distributions and color-color relations, as well as the exact forms of the color-metallicity transformations, in interpreting the observational data on GC color bimodality. Our ACS data for NGC 4874 shows that its GC system exhibits a very strong blue tilt, implying a very steep mass-metallicity scaling, and the centroid of this GC system is offset by $4{\pm}1kpc$ from the luminosity center of NGC 4874, in the direction of NGC 4872. Finally, I discuss the asymmetrical GC distribution around a dwarf elliptical galaxy in Coma that has a very high relative velocity with respect to the cluster mean at small clustercentric radius.

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Assessment on the East Asian Summer Monsoon Simulation by Improved Global Coupled (GC) Model (Global Coupled (GC) 모델 개선에 따른 동아시아 여름 몬순 모의성능 평가)

  • Kim, Ji-Yeong;Hyun, Yu-Kyung;Lee, Johan;Shin, Beom-Cheol
    • Atmosphere
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    • v.31 no.5
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    • pp.563-576
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    • 2021
  • The performance of East Asian summer monsoon is assessed for GC2 and GC3.1, which are climate change models of the current and next climate prediction system in the Korea Meteorological Administration (KMA), GloSea5 and GloSea6. The most pronounced characteristics of GC models are strong monsoon trough and the weakening of the Western North Pacific Subtropical High (WNPSH). These are related to the weakening of the southwesterly wind and resulting weak monsoon band toward the Korean Peninsula. The GC3.1 is known to have improved the model configuration version compared to GC2, such as cloud physics and ocean parameters. We can confirm that the overall improvements of GC3.1 against GC2, especially in pressure, 850 hPa wind fields, and vertical wind shear. Also, the precipitation band stagnant in the south of 30°N in late spring is improved, therefore the biases of rainy onset and withdrawal on the Korean Peninsula are reduced by 2~4 pentad. We also investigate the impact of initialization in comparison with GloSea5 hindcast. Compared with GCs, hindcast results show better simulation within 1 month lead time, especially in pressure and 850 hPa wind fields, which can be expected to the improvement of WNPSH. Therefore, it is expected that the simulation performance of WNPSH will be improved in the result of applying the initialization of GloSea6.

Expression of VP2 of Aquatic Birnavirus GC-1 Isolated from Rockfish (Sebastes schlegeli), Rearing in Seawater in Korea (국내 해산양식어 조피볼락에서 분리된 수생버나바이러스 GC-1의 VP2 발현)

  • Joh, Seong-joon;Sung, Haan-woo;Lee, Yun-jeong;Kim, Jae-hong;Kang, Shien-young
    • Korean Journal of Veterinary Research
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    • v.43 no.3
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    • pp.449-456
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    • 2003
  • The VP2 gene of aquatic birnavirus, Korean isolate (GC-1) was cloned and expressed using the baculovirus expression system. The VP2 gene and VP2 partial gene, which contained a neutralizing epitope, were constructed for recombinant transfer vectors, for baculovirus expression. The expressed recombinant proteins were confirmed by indirect immuno fluorescence antibody (IFA), SDS-PAGE and Western blot. The level of expression was checked at regular time using IFA and Western blot. To measure the neutralizing activity of recombinant proteins against GC-1 strain, the antisera against recombinant proteins were produced by using guinea pigs. The result showed that the antisera neutralized the GC-1 strain. However, the neutralizing titer was higher in antisera against the VP2 gene expressed recombinant protein than that of VP2 partial gene recombinant protein.

miR-124 Inhibits Growth and Invasion of Gastric Cancer by Targeting ROCK1

  • Hu, Cong-Bing;Li, Qiao-Lin;Hu, Jian-Fei;Zhang, Qiang;Xie, Jian-Ping;Deng, Li
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.16
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    • pp.6543-6546
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    • 2014
  • MicroRNAs (miRNAs) act as critical regulators of genes involved in many biological processes. Aberrant alteration of miRNAs have been found in many cancers, including gastric cancer (GC), but the molecular mechanisms are not well understood. Herein, we investigated the role of miR-124 in GC. We found that its expression was significantly reduced in both GC tissue samples and cell lines. Forced expression of miR-124 suppressed GC cell proliferation, migration, and invasion. Furthermore, the Rho-associated protein kinase (ROCK1) was identified as a direct target of miR-124 in GC cells. Finally, silencing of ROCK1 showed similar effects as miR-124 overexpression, while supplementation of ROCK1 remarkably restored the cell growth and invasion inhibited by miR-124. Together, our data demonstrate that miR-124 acts as a tumor suppressor by targeting ROCK1, and posit miR-124 as a novel strategy for GC treatment.

Genogroup position of aquabirnavirus GC-1 isolated from rockfish Sebastes schiegeli in Korea

  • Joh, Seong-Joon;Lee, Youn-Jeong;Song, Chang-Sun;Kang, Shien-Young;Mo, In-Pil;Heo, Gang-Jun
    • Korean Journal of Veterinary Research
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    • v.48 no.3
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    • pp.287-293
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    • 2008
  • The cDNA of the aquabirnavirus, GC-1 isolated from rockfish Sebastes schlegeli in Korea, was synthesized using the reverse transcriptase-polymerase chain reaction. The nucleotide and deduced amino acid sequences were determined from cDNA of the VP2-NS-VP3 coding region of genome segment A. The nucleotide sequences of the segment A were 3,086 base pairs (bp) in length and contained large open reading frame (ORF) and terminal sequences. The large ORF was comprised of 2,916 bp nucleotides and composed of 972 deduced amino acid sequences. Pairwise comparisons were made with other aquabirnavirus sequences published previously. The study of genetic relationships between GC-1 and aquabirnaviruses in the large ORF and VP2 coding regions demonstrated that the GC-1 has the nearest genetic relationship with the marine birnaviruses (MABV strains), and the GC-1 and MABV strains can be clustered as the same genogroup. GC-1 can be included in MABV, which is the 7th genogroup of family Aquabirnaviridae.

Pyrolysis/GC-Mass Spectrometry Analysis for Rapid Identification of Volatile Flavour Compounds of Accelerated Ripened Cheddar Cheese and Enzyme-Modified Cheese (단기숙성치즈 및 EMC 치즈의 휘발성 풍미성분 신속분석방법으로서 Pyrolysis/GC-Mass Spectrometry의 이용)

  • ;;;S.S.B. Haileselassie;V.A. Yaylayan;B.H. Lee
    • Food Science of Animal Resources
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    • v.21 no.3
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    • pp.256-264
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    • 2001
  • Pyrolysis/GC-mass spectrometry(Hewlet-Packard 5890GC/mass selective detector, 5971 BMSD), interfaced to a CDS Pyroprobe 1500 was optimized for rapid analysis of flavour compounds in Cheddar cheese. Twenty flavour compounds, including aldehydes(4), ketones(4), fatty acids(10), alcohol(1), and hydrocarbon(1), were identified from Cheddar cheeses. In total, Twenty-three flavour compounds aldehydes(2), ketones(8), alcohols(3), fatty acids(7), lactone(1), benzene derivative(1) and amide(1) were identified from two samples of accelerated-ripened Cheddar cheese treated with the proteolytic enzymes of Lactobacillus casei LGY. In total, Twenty-one flavour compounds; aldehydes(2), ketones(5), alcohols(2), fatty acids(11), and lactone(1) were identified from enzyme-modified cheese(EMC) treated with the combination of the proteolytic enzymes of Lactobacillus casei LGY and commercial endopeptidase or lipase. However, All the flavour compounds identified by pyrolysis/GC/MS in samples of ARC and EMC were not determined whether they are recognized as typical Cheddar flavour or not. More studies were requested on the development of methods for a rapid and convienent analysis of dairy fermented products using pyrolysis/GC-mass spectrometry.

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Repellency of the Constituents of Caraway Oil, Carum carvi against, Tetranychus urticae (점박이응애에 대한 Caraway Oil의 기피활성)

  • Yu Jeong-Su;Bae Jeong-Sook;Shin Dong-Ku;Kim Gil-Hah
    • Korean journal of applied entomology
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    • v.44 no.2
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    • pp.161-164
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    • 2005
  • Essential oils of three plants (caraway oil, hyssop oil and lime oil) were screened for repellency against Tetranychus urticae female adults in the lab condition. Among them, caraway oil (Carum carvi) showed $92.2\%$ repellency against female adults at 1,000 ppm concentration. Through the constituent analysis using GC and GC/MS, we confirmed that the two main constituents were carvone $(73.3\%)$ and limonene (26.7). Limonene showed 87.8 and $83.1\%$ repellency against adult females at 1,000 and 100 ppm concentrations, respectively.

Biological Control of Fusarium Stalk Rot of Maize Using Bacillus spp. (Bacillus spp.를 이용한 옥수수 밑둥썩음병의 생물학적 방제)

  • Han, Joon-Hee;Park, Gi-Chang;Kim, Joon-Oh;Kim, Kyoung Su
    • Research in Plant Disease
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    • v.21 no.4
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    • pp.280-289
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    • 2015
  • Maize (Zea mays L.) is an economically important crop in worldwide. While the consumption of the maize is steadily increasing, the yield is decreasing due to continuous mono-cultivation and infection of soil-borne fungal pathogens such as Fusarium species. Recently, stalk rot disease in maize, caused by F. subglutinans and F. temperatum has been reported in Korea. In this study, we isolated bacterial isolates in rhizosphere soil of maize and subsequently tested for antagonistic activities against F. subglutinans and F. temperatum. A total of 1,357 bacterial strains were isolated from rhizosphere. Among them three bacterial isolates (GC02, GC07, GC08) were selected, based on antagonistic effects against Fusarium species. The isolates GC02 and GC07 were most efficient in inhibiting the mycelium growth of the pathogens. The three isolates GC02, GC07 and GC08 were identified as Bacillus methylotrophicus, B. amyloliquefaciens and B. thuringiensis using 16S rRNA sequence analysis, respectively. GC02 and GC07 bacterial suspensions were able to suppress over 80% conidial germination of the pathogens. GC02, GC07 and GC08 were capable of producing large quantities of protease enzymes, whereas the isolates GC07 and GC08 produced cellulase enzymes. The isolates GC02 and GC07 were more efficient in phosphate solubilization and siderophore production than GC08. Analysis of disease suppression revealed that GC07 was most effective in suppressing the disease development of stalk rot. It was also found that B. methylotrophicus GC02 and B. amyloliquefaciens GC07 have an ability to inhibit the growth of other plant pathogenic fungi. This study indicated B. methylotrophicus GC02 and B. amyloliquefaciens GC07 has potential for being used for the development of a biological control agent.