• Journal of Ginseng Research
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• 제40권4호
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• pp.453-461
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• 2016

Background: This study aims to describe the characterization of Paenibacillus polymyxa GBR-1 (GBR-1) with respect to its positive and negative effects on plants. Methods: The morphological characteristics of GBR-1 were identified with microscopy, and subjected to Biolog analysis for identification. Bacterial population and media optimization were determined by a growth curve. The potential for GBR-1 as a growth promoting agent, to have antagonistic activity, and to have hydrolytic activity at different temperatures was assessed. The coinoculation of GBR-1 with other microorganisms and its pathogenicity on various stored plants, including ginseng, were assessed. Results: Colony morphology, endospore-bearing cells, and cell division of GBR-1 were identified by microscopy; identification was performed by utilizing the Biolog system, gas chromatography of fatty acid methyl esters (GC-FAME). GBR-1 showed the strongest antagonistic activity against fungal and bacterial pathogens. GBR-1 cell numbers were relatively higher when the cells were cultured in brain heart infusion (BHI) medium when compared with other media. Furthermore, the starch-hydrolytic activity was influenced by GBR-1 at higher temperature compared to low temperatures. GBR-1 was pathogenic to some of the storage plants. Coinoculation of GBR-1 with other pathogens causes differences in rotting on ginseng roots. A significant growth promotion was observed in tobacco seedlings treated with GBR-1 suspensions under in vitro conditions, suggesting that its volatile organic compounds (VOCs) might play a role in growth promotion. Conclusion: The results of this study indicate that GBR-1 has both positive and negative effects on ginseng root and other stored plants as a potential biocontrol agent and eliciting in vitro growth promotion.

• Journal of Korean Dental Science
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• 제17권2호
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• pp.53-63
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• 2024

Purpose: To investigate the effects of simultaneous soft and hard tissue augmentation and the addition of polydeoxyribonucleotide (PDRN) on regenerative outcomes. Materials and Methods: In five mongrel dogs, chronic ridge defects were established in both mandibles. Six implants were placed in the mandible, producing buccal dehiscence defects. The implants were randomly allocated to one of the following groups: 1) control: no treatment; 2) GBR: guided bone regeneration (GBR) only; 3) GBR/PDRN: GBR+PDRN application to bone substitute particles; 4) GBR/CTG: GBR+connective tissue grafting (CTG); 5) GBR/VCMX: GBR+soft tissue augmentation using volume stable collagen matrix (VCMX); and 6) group GBR/VCMX/PDRN: GBR+VCMX soaked with PDRN. The healing abutments were connected to the implants to provide additional room for tissue regeneration. Submerged healing was achieved. The animals were euthanized after four months. Histological and histomorphometric analyses were then performed. Results: Healing abutments were gradually exposed during the healing period. Histologically, minimal new bone formation was observed in the dehiscence defects. No specific differences were found between the groups regarding collagen fiber orientation and density in the augmented area. No traces of CTG or VCMX were detected. Histomorphometrically, the mean tissue thickness was greater in the control group than in the other groups above the implant shoulder (IS). Below the IS level, the CTG and PDRN groups exhibited more favorable tissue thickness than the other groups. Conclusion: Failure of submerged healing after tissue augmentation deteriorated the tissue contour. PDRN appears to have a positive effect on soft tissues.

• 한국식생활문화학회지
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• 제33권4호
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• pp.337-344
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• 2018

Germinated brown rice (GBR, Orysa sartiva L.) has been reported to have anti-obesity and anti-inflammatory effects. However, the mechanisms underlying these effects in adipocytes are not fully understood. Therefore, this study was conducted to explore the anti-inflammatory mechanisms of GBR on lipopolysaccharide (LPS)-stimulated 3T3-L1 adipocytes. 3T3-L1 adipocytes were pretreated with GBR extracts (0-20 mg/mL) 1 h before LPS stimulation. The mRNA expression of adipokines and Toll-like receptor 4 (TLR4) were measured by RT-PCR. The protein expressions of TLR4-related molecules were detected by western blotting and nuclear factor-${\kappa}B$ ($NF-{\kappa}B$) activation was measured. Our results showed that GBR extract dose-dependently inhibited mRNA expression of LPS-induced tumor necrosis factor-${\alpha}$ ($TNF-{\alpha}$), interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1). GBR extract was found to inhibit LPS-induced mRNA expression of TLR4 and protein expression of both myeloid differentiation factor 88 (MyD88) and TNF receptor-associated factor 6 (TRAF6). Furthermore, GBR extract significantly inhibited extracellular receptor-activated kinase (ERK) phosphorylation and $NF-{\kappa}B$ activation. These results suggest that GBR extract has the anti-inflammatory effects on LPS-induced inflammation via inhibition of TLR4 signaling, includingthe ERK and $NF-{\kappa}B$ signaling pathways, in adipocytes.

• 대한본초학회지
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• 제31권5호
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• pp.85-92
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• 2016

Objectives： This study is experimental comparison of brown rice (BR) and germinated brown rice (GBR) on upper gastrointestinal diseases animal models.Methods： The ICR mice were divided randomly into four groups of six animals each (Normal mice, gastritis mice, gastritis mice treated with BR, gastritis mice treated with 48h GBR). Gastritis was induced by administration of 0.5 mL 150 mM HCl-60% ethanol. Six-week-old male Sprague-Dawley (SD) rats were randomly divided into 7 groups after 1 week adaptation. (Normal rat, reflux esophagitis (RE) rat, RE rat treated with BR, RE rat treated with 24,30,36,48h GBR). Reflux esophagitis was induced by ligation with a 2-0 silk thread both the pylorus and the transitional junction between the forestomach and the corpus in SD rats.Results： HCl/ethanol-induced gastric mucosal injury mice were ameliorated mucosal damage upon histological evaluation by treatment of 48h GBR than BR. Optical changes such as hyperemia and multiple erosions were observed in the rats with RE and damage to the normal rats was not apparent. The oral administration of GBR significantly diminished against gross mucosal damage in a germination time-dependent manner. Also, the administration of GBR suppressed the biomarker of oxidative stress, reactive oxygen species (ROS) and produces peroxynitrite (ONOO-) in serum. However, the administration of GBR could not affect to the pH level secreted from stomach when compared with Control group.Conclusions： These findings suggest that GBR could have improving effects on upper gastrointestinal diseases in a germination time-dependent manner.

• 한국식품조리과학회지
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• 제31권6호
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• pp.781-790
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• 2015

Germinated brown rice (GBR) flour (0%, 25%, 50%, 75% and 100%) was added to brown rice (BR) and white rice (WR) flours and the densities of pound cake batters, morphology, color value, textural properties, sensory preference test, and hardness during storage were investigated, in order to develop health functional gluten-free rice pound cakes with high nutritional value. The rice was milled with the newly developed rice milling method. The entire shape and cross section of all cakes were symmetrical and desirable. The density of pound cake batter and weight of cake increased with addition of increasing GBR. Specific volume was not significantly different (2.1-2.4 mL/g). Textural properties of cakes between WR-GBR and BR-GBR showed different trends with adding GBR. On the sensory test, overall quality of rice pound cake did not significantly differ between rice flour types. Hardness of rice pound cakes and brittleness decreased with increasing addition of GBR flour. The hardness and the peak intensity at $2{\theta}=17^{\circ}$ decreased with increasing addition of GBR. The results, that addition of GBR flour improved the nutrition value and functionality of gluten free rice pound cakes and prevented staling of rice pound cakes during storage.

• Journal of Periodontal and Implant Science
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• 제51권6호
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• pp.433-443
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• 2021

Purpose: This study aimed to evaluate the effectiveness of the partial split-flap technique with a K-incision on vertical guided bone regeneration (vGBR) and to retrospectively analyze the clinical and radiographic outcomes of dental implantation using this approach. Methods: In total, 78 patients who received 104 dental implants with vGBR, categorized as (1) pre-GBR and post-implantation and (2) simultaneous GBR and implantation, were enrolled. Data analysis was based on periapical radiographs, clinical photos, and dental records. The 2-sample t-test was used to compare the 2 surgical procedures. Results: The baseline vertical bone level, augmented bone height (ABH), and treatment duration were significantly higher in the pre-GBR procedure group. The survival rates of the implants were 96.1% and 94.8% in implant- and patient-based analyses, respectively. In Cox regression analysis, high rates of implant failure were found in the presence of ABH of ≥4 mm, smoking, and diabetes. Conclusions: Within the limitations of this retrospective study, the partial split-flap technique using a K-incision for vGBR showed stable clinical outcomes and favorable dental implant survival.

• 한국식품과학회지
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• 제38권5호
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• pp.674-678
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• 2006

발아현미의 섭취에 의한 흰쥐의 비만억제 및 콜레스테롤 저하 효과를 조사하기 위하여 대조군, 백미군, 현미군, 발아현미군의 4가지 처리군으로 나누어 흰쥐에 고지방, 고콜레스테롤 식이를 공급하면서 5주간 사육후 효과를 비교하였다. 체중의 경우 모든 처리군에서 유의적인 차이를 나타내지 않았으나 발아현미군에서 5주 경과후의 체중에서 $471.2{\pm}17.8\;g$으로 가장 작은 값을 나타내었다. 체중증가량과 식이효율의 경우에도 발아현미군이 각각 $310.6{\pm}14.7\;g$과 $41.8{\pm}2.0%$로 유의적인 수준은 아니지만 가장 낮은 값을 나타내었다. 혈중 중성지질 및 콜레스테롤의 경우 발아 현미군은 5주 경과시 각각 $54.0{\pm}23.4\;mg/dL$, $64.8{\pm}14.7\;mg/dL$로 백미군, 현미군에 비해 크게 낮을 뿐만 아니라 부고환지방, 신장 지방 중량에서도 낮은 값을 나타내었다. 간의 총 지질과 총 콜레스테롤에서 발아현미군은 $216.3{\pm}35.7\;mg/g$ liver 와 $16.5{\pm}0.7\;mg/g$ liver로 기타 처리군에 비해 낮은 값을 나타내었으며, 변의 총 지질, 총 콜레스테롤, 중성지질에서도 각각 $165.0{\pm}22.2\;mg/g$ feces, $26.5{\pm}2.7\;mg/g$ feces, 3.4 mg/g feces로 기타 처리군에 비해 유의적으로 높은 값을 나타내었다.

• 한국식품조리과학회지
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• 제26권6호
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• pp.853-859
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• 2010

발아현미 100%인 control에 대한 적합가능성과 control에 멥쌀의 적정 첨가 비율을 찾는 목적으로 발아현미와 멥쌀의 수분흡수율과 고형분 함량을 측정하고 발아현미 가래떡의 품질특성을 조사하여 제품으로 가장 적합한 조건을 찾고자하였다. 발아현미 가래떡은 멥쌀 첨가량을 발아현미 중량 대비 0, 20, 40, 60%로 제조하여 실온($25^{\circ}C$)에서 0, 1, 2일 저장하면서 실험을 진행하였다. 멥쌀 및 발아현미의 수분흡수율과 고형분 함량을 조사한 결과 수침전의 수분함량은 멥쌀의 경우 15.68%, 발아현미는 15.05% 이었으며 시간이 경과함에 따라 지속적으로 증가하였다. 수침시간에 따른 멥쌀과 발아현미의 고형분 함량은 2시간 후에는 각각 0.68% 및 0.71%이었고 시간이 경과함에 따라 10시간 후에는 0.76%와 0.79%로 양이 증가하였다. 발아현미 가래떡의 수분함량의 변화는 멥쌀 첨가량이 증가할수록 가래떡의 수분함량은 1~3%감소하는 수준을 보였으며 저장기간 동안에는 모든 시료에서 감소하는 경향을 나타내었다. 색도의 변화의 경우 L값은 발아현미 첨가량이 높아질수록 가래떡의 색이 어두워지는 경향이었으며 모든 시료간의 차이를 나타내었다. a값과 b값의 경우 발아현미 첨가량이 많아질수록 높은 값을 나타내었다. 조직감을 측정한 결과 경도는 40% 및 60%가 높았으며, 탄력성은 control이 좋은 점수를 받았으며, 응집성, 검성 및 씹힘성은 60%가 높은 값을 보였다. 멥쌀 첨가비율에 따른 발아현미 가래떡의 관능적 특성에서 제조 당일 발아현미 첨가량이 감소할수록 향미를 제외한 색, 단단한 정도, 탄력성, 씹힙성, 전반적기호도 등 모든 평가항목에서 높게 평가되었으며 저장기간이 증가할수록 멥쌀 40%와 60% 첨가군에서 우수하게 평가되었다. 따라서 가래떡 제조 시 발아현미 100%인 control도 제조 가능하고 발아현미에 멥쌀을 첨가할 경우 40~60% 첨가하는 것이 가장 선호하는 것으로 나타났다.

• 동아시아식생활학회지
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• 제21권5호
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• pp.698-705
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• 2011

In this study, brown rice and germinated brown rice which has various medical action and excellent functionality, was used together with Jeung-pyun to compare and analyze the changes according to the amount 50, 100%. This study is intended to be used for the healthy food and spread to that was executed to find out the merchandising potential of this Jeung-pyun. The Jeung-pyun was added to brown rice and germinated brown rice of moisture content was 50.23~51.70% the similar to content. For chromaticity to L(lightness) value, a(redness) value and b(yellowness) value, L value of GBR2 was low at 58.84. a value was showed the highest GBR at 0.64. For texture, Hardness was showed the highest GBR2. Springiness was showed the highest BR2. Chewiness was showed the highest Control at 4.33. As for adhesiveness, there was showed the highest GBR2 at 0.30 and showed significant difference between samples. Overall-acceptability was the order of good in the estimated: GBR1>BR2>BR1>GBR2>C. Based on this study, from the physico-chemical and sensual perspective, For the preference of GBR1 was most preferred for flavor, color and taste, sensory evalution and considered suitable the best method for adding brown rice and germinated brown rice into Jeung-pyun.

• Journal of Periodontal and Implant Science
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• 제37권3호
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• pp.543-551
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• 2007

When clinicians faced with an insufficient volume of supporting bone on ideally esthetic and bio-mechanical position for dental implantation, guided bone regeneration(GBR) was indicated. Although GBR has wide application at clinic, proper time of membrane removal remains qustionable in using non-resorbable membrane, such as non-expanded polytetrafluoroethylene(PTFE), The aim of this study was to compare the effect of maintenance period of PTFE membrane on bone regeneration in rabbit calvarial defects. Eight adult New Zealand white female rabbits were used in this study. Four defects were surgically made in their calvaria. Using a trephine bur, 4 'through and through' defects were created and classified into 3 groups, which were consisted of control group(no graft), experimental group 1(autogenous bone)and experimental group 2(deproteinized bovine bone; $OCS-B^{(R)}$). The defects were covered with PTFE membrane($Cytoplast^{(R)}$). Membranes were removed after 1, 2, 4 and 8 weeks post-GBR procedure in 2 rabbits repectively, All rabbits were sacrificed after 8 week post-GBR procedure. Specimens were harvested and observed histologically. The results were as follow; 1) The use of graft material and membrane was necessary in GBR procedure. 2) When PTFE membranes were removed early, the most favorable bone regeneration was revealed in experimental group T, followed by experimental group II and control group. 3) On GBR, it is recommended that membrane should maintain for 4 weeks with autogenous graft. As well, the use of xenograft need longer maintenance period than autogenous bone. Further evaluations will be needed, such as histomorphologic research, more species and different kinds of graft materials. And on the basis of these studies, clinical researches would be required.