• Journal of Plant Biotechnology
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• v.31 no.2
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• pp.163-167
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• 2004

The effects of sucrose concentration on the secretion of hGM-CSF, total protein and protease into the medium were investigated in transgenic tobacco cells. The dry cell weight (11.22 g/L), hGM-CSF (181.53 $\mu\textrm{g}$/L) and total protein (66.8 mg/L) were detected as highest at 30 g/L sucrose and protease activity (2660 U/L) was highest at 120 g/L sucrose after 5-day culture. However after 10-day culture, the maximum dry cell weight (28.36 g/L) was found at 60 g/L sucrose while the maximum hGM-CSF (95 $\mu\textrm{g}$/L) was appeared at 150 g/L sucrose. The total protein and protease activity was secreted as 52.28mg/L and 3430 U/L, respectively in the same culture.

• Korean Journal of Food Science and Technology
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• v.21 no.5
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• pp.619-623
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• 1989

The cultural condition of Pseudomonas mendocina for polysaccharide production was examined. The optimal medium contains following composition per liter of distilled water: Sucrose 23.75g, $(NH_4)_2SO_4$ 1.57g, Yeast extract 0.5g, $KH_2PO_4\;2.9g,\;MgSO_4.\;7H_2O\;1.0g,\;CaCO_3$ 2.5g. The optimum temperature and pH were $30^{\circ}C$ and 6.5. At the condition. Ps mendocina produced 5.98g/l of polysaccharide. The culture viscosity after 3 days was 191mPa.s at $70sec^{-1}$. The product yield $(Y_{p/s})$ and specific productivity $(Q_p)$ were 25.18% and 32.83mg/g-cell/h.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.25 no.10A
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• pp.1560-1565
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• 2000

Over an additive abelian group G of order g and for a given positive integer λ, a generalized Hadamard matrix GF(g,λ) is defined as a gλ$\times$gλ matrix [h(i,j)] where 1$\leq$i$\leq$gλ,1$\leq$j$\leq$gλ, such that every element of G appears exactly λ times in the list h(i$_1$,1)-h(i$_2$,1), h(i$_1$,2)-h(i$_2$,2),...,h(i$_1$,gλ)-h(i$_2$, gλ) for any i$\neq$j. In this paper, we propose a new method of expanding a GH(\ulcorner,λ$_1$) = B = \ulcorner over G by replacing each of its m-tuple \ulcorner with \ulcorner GH(g,λ$_2$) where m=gλ$_2$. We may use \ulcornerλ$_1$(not necessarily all distinct) GH(g,λ$_2$)'s for the substitution and the resulting matrix is defined over the group of order g.

• Journal of applied mathematics & informatics
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• v.32 no.3_4
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• pp.503-509
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• 2014

For a connected graph G, there are orientations of G have different hull numbers, geodetic numbers, and convexity numbers. The lower orientable hull number $h^-(G)$ is defined as the minimum hull number among all the orientations of G and the upper orientable hull number $h^+(G)$ as the maximum hull number among all the orientations of G. The lower and upper orientable geodetic numbers $g^-(G)$ and $g^+(G)$ are defined similarily. In this paper, We investigate characterizations of the orientable numbers and the conditions that the relation $h^-(G){\leq}g^-(G)$ < $h^+(G){\leq}g^+(G)$ holds.

• Journal of the Korean Wood Science and Technology
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• v.38 no.6
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• pp.561-567
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• 2010

In this study, we evaluated optimal conditions for ethanol production of the spruce hydrolysate (SH) obtained from diethyl oxalate pretreatment. Fermentable sugar concentration in SH was 29.04 g/${\ell}$ except arabinose. Monosaccharides obtained from the oligomer degradation were mainly mannose (39.26 g/${\ell}$) and galactose (12.83 g/${\ell}$). Concentration of 5-HMF and furfural which are inhibitors on ethanol fermentation were 0.09 g/${\ell}$ and 0.04 g/${\ell}$ respectively. Concentration of acetic acid and total phenolic compounds in SH were 1.4 g/${\ell}$ and 2.83 g/${\ell}$. Ethanol production using hydrolysate was 11.7 g/${\ell}$ at optimal pH 6.0 after 48 h. Specific ethanol production was 0.15 (g/(${\ell}^*h$)) at pH 5.0 and 5.5. while that was 0.24 (g/(${\ell}^*h$)) at pH 6.0. Specific ethanol production has difference depend on initial pH for fermentation. Ethanol production was 14.3 g/${\ell}$ after 48 h when xylanase 20 IU was added in SH for degradation of oligomer during fermentation. It implied that ethanol production increased by 22.2% compare with control (without xylanase).

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.9 no.3
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• pp.266-272
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• 2016

In this study, we analysed the technology convergence of Internet of Things(IoT) by International Patent Classification(IPC) code analysis. 163 patent applications in Korea are the subjects of this study. We confirmed that the most representative IPC code combinations between Main and Sub categories are G06Q 50/24-G06Q 50/22(6 cases), H04L 29/02-H04L 12/28(4 cases), G06F 15/16-G06F 3/048(3 cases), G06F 15/16-G06F 9/44(3 cases), and G06Q 50/22-G06Q 50/24(3 cases). The field of Health Care business have been prepared 9 patent applications by technology convergence between 'Health Care(G06Q 50/22)' and 'Patient Record Management(G06Q 50/24)'. Finally we also concluded that the core IPC code are G06F 15/16, G06Q 50/22, G06Q 50/24, and H04L 12/28 by the technology convergence interconnections analysis of IoT patent applications.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.15 no.5
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• pp.308-314
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• 2022

This study deals with the technology Convergence Analysis by IPC Code-Based Social Network Analysis of Healthcare Patents filed in Korea. The relationship between core technologies is visualized using Social Network Analysis. At the subclass level of healthcare patents, 1,155 cases (49.4%) of patents with complex IPC codes were investigated, and as a result of Social Network Analysis on them, the IPC codes with the highest Degree Centrality were A61B, G16H, and G06Q, in that order. The IPC codes with the highest Betweenness Centrality are in the order of A61B, G16H, and G06Q. In addition, it was confirmed that healthcare patents consist of two large technology clusters. Cluster-1 corresponds to related business models centered on A61B, G16H and G06Q, and Cluster-2 is consisting of H04L, H04W and H04B. The technology convergence core pairs of the healthcare patent is [G16H-A61B] and [G16H-G06Q] in Cluster-1, and [H04L-H04W] in Cluster-2. The results of this study can contribute to the development of core technologies for healthcare patents.

• Nutrition Research and Practice
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• v.11 no.1
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• pp.11-16
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• 2017

BACKGROUND/OBJECTIVES: Helicobacter pylori (H. pylori) colonization of the stomach mucosa and duodenum is the major cause of acute and chronic gastroduodenal pathology in humans. Efforts to find effective anti-bacterial strategies against H. pylori for the non-antibiotic control of H. pylori infection are urgently required. In this study, we used whey to prepare glycomacropeptide (GMP), from which sialic acid (G-SA) was enzymatically isolated. We investigated the anti-bacterial effects of G-SA against H. pylori in vitro and in an H. pylori-infected murine model. MATERIALS/METHODS: The anti-bacterial activity of G-SA was measured in vitro using the macrodilution method, and interleukin-8 (IL-8) production was measured in H. pylori and AGS cell co-cultures by ELISA. For in vivo study, G-SA 5 g/kg body weight (bw)/day and H. pylori were administered to mice three times over one week. After one week, G-SA 5 g/kg bw/day alone was administered every day for one week. Tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), IL-$1{\beta}$, IL-6, and IL-10 levels were measured by ELISA to determine the anti-inflammatory effects of G-SA. In addition, real-time PCR was performed to measure the genetic expression of cytotoxin-associated gene A (cagA). RESULTS: G-SA inhibited the growth of H. pylori and suppressed IL-8 production in H. pylori and in AGS cell co-cultures in vitro. In the in vivo assay, administration of G-SA reduced levels of IL-$1{\beta}$ and IL-6 pro-inflammatory cytokines whereas IL-10 level increased. Also, G-SA suppressed the expression of cagA in the stomach of H. pylori-infected mice. CONCLUSION: G-SA possesses anti-H. pylori activity as well as an anti-H. pylori-induced gastric inflammatory effect in an experimental H. pylori-infected murine model. G-SA has potential as an alternative to antibiotics for the prevention of H. pylori infection and H. pylori-induced gastric disease prevention.

• Journal of Environmental Science International
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• v.17 no.2
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• pp.233-237
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• 2008

The objective of this study was conducted to evaluate the effects of poultry litter amendments on pH and soluble reactive phosphorus (SRP) in poultry litter. Two laboratory studies were conducted for 42 d in Exp. 1 and for 10 d in Exp. 2, respectively. The poultry litter was treated with various amendments which included 4 g fly ash and 4 g $AlCl_3\;(AlCl_36H_2O)/100g$ litter in Exp. 1 and 4 g alum$(Al_2(SO_4){_3}\;14H_2O)$, 8 g alum, 8.66 g liquid alum, and 17.3 g liquid alum/100 g litter in Exp. 2; untreated litter served as controls. There were no differences in pH between control and T1(4 g fly ash) and SRP contents between T1(4 g fly ash) and T2(4 g $AlCl_3$) in Exp. 1. A significant difference in pH and SRP contents in Exp. 2 was observed among all treatments(P< 0.05). In experiment 1, T1(4 g fly ash) and T2(4 g $AlCl_3$) at 42 d decreased SRP in litter by 47.1% and 62.6% of that from litter alone, respectively. In experiment 2, T1(4 g alum), T2(8.66 g liquid alum), T3(8 g alum), and T4(17.3 g liquid alum) treatments at 10 days reduced SRP contents by up to 36.2%, 62.9%, 87.0%, and 83.9%, respectively, when compared with the controls. Decrease in SRP contents was chiefly associated with reduction in litter pH. These results indicate that use of various litter amendments to limit P solubility has potential and should be pursued as a means of reducing soluble reative phosphorus during short term.

• Microbiology and Biotechnology Letters
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• v.19 no.5
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• pp.487-490
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• 1991

In this study continuous alcohol fermentation of molasses by the recirculation system has investigated. After cultivation of yeast cells in the YPD medium with increasing the medium concentration from 10 to 183.5 g/l stepwisely, the fermentation medium was replaced by molasses. The maximum cell mass was 25 g/l, and the mean cell mass during the operation was 23.5g/1, which was 3.4 times higher compared with a conventional batch system. The optimum fermentation conditions with feeding molasses of 180 g/l were obtained when the fermentation was carried out at 500 rpm and at the dilution rate of 0.037 $h^{-1}$. Under these conditions we could safely operate the fermentor for 645 h without any trouble. The maximum alcohol productivity was 4.9 g$l\cdot h$ with an alcohol concentration of 53.9 g/l at the dilution rate of 0.091$h^{-1}$.