• Asian Pacific Journal of Cancer Prevention
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• v.15 no.12
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• pp.4897-4902
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• 2014

Purpose: To investigate the anticancer effects and underlying mechanisms of parthenolide on HepG2 human hepatocellular carcinoma cells. Materials and Methods: Cell viability was assessed by MTT assay and cell apoptosis through DAPI, TUNEL staining and Western blotting. Monodansylcadaverin(MDC) and AO staining were used to detect cell autophagy. Cell proliferation was assessed by Ki67 immunofluorescence staining. Results: Parthenolide induced growth inhibition in HepG2 cells. DAPI and TUNEL staining showed that parthenolide could increase the number of apoptotic nuclei, while reducing the expression of the anti-apoptotic protein Bcl-2 and elevating the expression of related proteins, like p53, Bax, cleaved caspase9 and cleaved caspase3. Parthenolide could induce autophagy in HepG2 cells and inhibited the expression of proliferation-related gene, Ki-67. Conclusions: Parthenolide can exert anti-cancer effects by inducing cell apoptosis, activating autophagy and inhibiting cell proliferation.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.14 no.2
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• pp.104-109
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• 2003

The purpose of this study is to analyze the EGG measures from Lx Speech Studio program (Laryngograph Ltd, UK) in patient with vocal nodule. Thirty female adults (15 patient with vocal nodule, 15 normal speaker) produced sustained vowel and read the passage. They were grouped into three groups based on Grade (GRBAS) : normal-G0, nodule-Gl, nodule-G2. Estimates of Fx (Hz), Qx(%), Jitter, Shimmer, and HNR were made from a 500msec midportion of vowel. In addition, DFx(Hz), DQx(%), CFx(%) and CAx(%) were obtained from reading the passage. These data were compared among groups. The results were as follow Jitter, Shimmer, HNR were significantly higher in nodule-G2 group than in normal-G0 & patient-Gl group. In nodule-G2 group, CFx and CAx from reading passage were significantly higher. For patients with nodule, asymmetry or irregularity were observed in graphs of QxFx ＆ CFx provided by Quantitative Analysis.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.5
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• pp.772-775
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• 1999

A six week growth performance trial involving 450 birds was conducted to determine the dietary requirement of broiler chicks for available methionine (AM). Body weight gain was significantly (p<0.01) lower on high AM diet (1411 g/bird) than low AM (1470 g/bird) and normal AM (1466 g/bird) diets. The feed intake by birds ranged from $3241{\pm}25.69$ in high AM diet to $3321{\pm}25.69g/bird$ in low AM diet. The feed efficiency for the three diets having low, normal and high level of AM were $2.26{\pm}0.02$, $2.24{\pm}0.02$ and $2.30{\pm}0.02$, respectively. The results indicated that the birds fed high AM diet consumed significantly (p<0.01) more feed per unit body weight gain as compared to birds on low and normal AM diets.

• Nutrition Research and Practice
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• v.1 no.1
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• pp.52-56
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• 2007

This research analyzed the iron status, clinical symptoms and physical characteristics between normal and anemic group of middle school girls in the Ulsan metropolitan area. It was carried out with 237 subjects (normal 190, anemic subject 47). They were evaluated with a questionnaire and measurement of hematological indices. BMI $(kg/m^2)$ of the two groups were $19.54{\pm}2.44$ (normal girls) and $19.22{\pm}2.27$ (anemic girls). The hemoglobin concentration of the anemic girls were $10.84{\pm}1.17g/dl$ and the serum iron of the anemic girls represent $35.15{\pm}27.47{\mu}g/100ml$. The TIBC (Total Iron Binding Capacity) of the anemic girls showed significantly high to $449.30{\pm}64.87{\mu}g/100ml$. The serum ferritin of the anemic girls was $20.53{\pm}42.29{\mu}g\ell$, it represented significantly low. The symptom of 'pale face' of the anemic girls were higher than the normal girls. Hemoglobin and serum iron were negatively correlated with 'pale face'. The TIBC was negatively correlated with 'Get a cold easily'. The duration and amount of menstruation were correlated with iron status. This research is to be utilized as basic data for dietary support and nutritional education to improve their iron status.

• Journal of the Chungcheong Mathematical Society
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• v.4 no.1
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• pp.103-113
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• 1991

Some properties of a path space and the fundamental group ${\sigma}(X,x_0,G)$ of a topological transformation group (X, G, ${\pi}$) are described. It is shown that ${\sigma}(X,x_0,H)$ is a normal subgroup of ${\sigma}(X,x_0,G)$ if H is a normal subgroup of G ; Let (X, G, ${\pi}$) be a transformation group with the open action property. If every identification map $p:{\Sigma}(X,x,G)\;{\longrightarrow}\;{\sigma}(X,x,G)$ is open for each $x{\in}X$, then ${\lambda}$ induces a homeomorphism between the fundamental groups ${\sigma}(X,x_0,G)$ and ${\sigma}(X,y_0,G)$ where ${\lambda}$ is a path from $x_0$ to $y_0$ in X ; The space ${\sigma}(X,x_0,G)$ is an H-space if the identification map $p:{\Sigma}(X,x_0,G)\;{\longrightarrow}\;{\sigma}(X,x_0,G)$ is open in a topological transformation group (X, G, ${\pi}$).

• Environmental Analysis Health and Toxicology
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• v.22 no.3
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• pp.263-269
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• 2007

Chitosan is a depolymerized and partially deacetylated derivative of chitin. We investigated the cytotoxicity of chitosan in cancer cell lines, such as P388, L1210, HCT-15, SK-HepG-1 and mouse splenocytes as a normal cell by MTT assay. To clarify whether chitosan enhances cytotoxicity of anticancer drugs, we also examined the cytotoxicity of combined treatment with chitosan and anticancer drugs, such as cisplatin, mitomycin C, and 5-fluorouracil in cancer cell lines in vitro. Chitosan ($37.5\;{\mu}g/mL,\;75\;{\mu}g/mL,\;112.5\;{\mu}g/mL,\;and\;150\;{\mu}g/mL$) showed concentration-dependent cytotoxicity in the cancer cell lines. In addition, chitosan showed relatively lower cytotoxicity in normal cells than in the cancer cell lines. Particularly, this trend was significant at high doses of chitosan, i.e. $112.5\;{\mu}g/mL,\;and\;150\;{\mu}g/mL$. Thus, these results suggest that chitosan may selectively induce the growth inhibition in cancer cell lines, compared to normal cells. Furthermore. the co-treatment of chitosan and anticancer drugs exhibited an apparant synergistic cytotoxicity in murine lymphoma cell lines, i.e. P388 and L1210 at $37.5\;{\mu}g/mL$ of chitosan rather than at $75\;{\mu}g/mL$ of chitosan, but such phenomenon could not be observed in solid tumor cell lines, i.e. HCT-15 and SK-HepG-1. However, chitosan did'nt reduced the cytotoxicity against normal mouse splenocytes induced by anticancer drugs. Therefore, it is concluded that the combination of chitosan and anticancer drugs might be useful for the cancer chemotherapy.

• Biomolecules & Therapeutics
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• v.5 no.4
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• pp.380-383
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• 1997

CJ-50001 is a recombinant granulocyte-colony stimulating factor (rG-CSF) developed by Cheil Jedang R&D Center. The effects of CJ-50001 on the increase of peripheral neutrophil count following intravenous and subcutaneous single administration at a dose of 20$\mu$g/kg in normal ICR mice and SD rats, respectively, were compared with those of Grasin, a control drug. Both CJ-50001 and Grasin significantly increased the peripheral neutrophil number in four treatment groups and the maximum number of neutrophil was achieved at 12 to 18 h in rats and mice, respectively. The dose dependency test was studied for CJ-50001 only in normal mice by intravenous or subcutaneous administration. When administered i.v or s.c at the various doses in normal mice, CJ-50001 significantly increased the neutrophil number over the dose of 160 ng/kg, compared with the vehicle control group. From these results, it was concluded that CJ-50001 showed efficacy similar to Grasin in the peripheral neutrophil count increase.

• Bulletin of the Korean Mathematical Society
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• v.53 no.6
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• pp.1685-1695
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• 2016

In this article we consider Lie super-bialgebra structures on the generalized loop super-Virasoro algebra ${\mathcal{G}}$. By proving that the first cohomology group $H^1({\mathcal{G}},{\mathcal{G}}{\otimes}{\mathcal{G}})$ is trivial, we obtain that all such Lie bialgebras are triangular coboundary.

• Journal of Microbiology and Biotechnology
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• v.34 no.5
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• pp.1178-1187
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• 2024

Cordyceps militaris is a significant edible fungus that produces a variety of bioactive compounds. We have previously established a uridine/uracil auxotrophic mutant and a corresponding Agrobacterium tumefaciens-mediated transformation (ATMT) system for genetic characterization in C. militaris using pyrG as a screening marker. In this study, we constructed an ATMT system based on a dual pyrG and hisB auxotrophic mutant of C. militaris. Using the uridine/uracil auxotrophic mutant as the background and pyrG as a selection marker, the hisB gene encoding imidazole glycerophosphate dehydratase, required for histidine biosynthesis, was knocked out by homologous recombination to construct a histidine auxotrophic C. militaris mutant. Then, pyrG in the histidine auxotrophic mutant was deleted to construct a ΔpyrG ΔhisB dual auxotrophic mutant. Further, we established an ATMT transformation system based on the dual auxotrophic C. militaris by using GFP and DsRed as reporter genes. Finally, to demonstrate the application of this dual transformation system for studies of gene function, knock out and complementation of the photoreceptor gene CmWC-1 in the dual auxotrophic C. militaris were performed. The newly constructed ATMT system with histidine and uridine/uracil auxotrophic markers provides a promising tool for genetic modifications in the medicinal fungus C. militaris.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.6
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• pp.2485-2489
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• 2014

In the present study, we studied the hypermethylation of the human riboflavin transporter 2 (hRFT2) gene and regulation of protein expression in biopsies from resected tissues from Uighur cervical squamous cell carcinoma (CSCC) patients and their neighboring normal tissues. hRFT2 gene promoter region methylation sequences were mapped in cervical cancer cell line SiHa by bisulfite-sequencing PCR and quantitative detection of methylated DNA from 30 pairs of Uighur's CSCCs and adjacent normal tissues by MassARRAY (Sequenom, San Diego, CA, USA) and hRFT2 protein expression was analyzed by immunohistochemistry. In SiHa, we identified 2 CG sites methylated from all of 12CpG sites of the hRFT2 gene. Analysis of the data from quantitative analysis of single CpG site methylation by Sequenom MassARRAY platform showed that the methylation level between two CpG sites (CpG 2 and CpG 3) from CpG 1~12 showed significant differences between CSCC and neighboring normal tissues. However, the methylation level of whole target CpG fragments demonstrated no significant variation between CSCC ($0.476{\pm}0.020$) and neighboring normal tissues ($0.401{\pm}0.019$, p>0.05). There was a tendency for translocation the hRFT2 proteins from cytoplasm/membrane to nucleus in CSCC with increase in methylation of CpG 2 and CpG 3 in hRFT2gene promoter regions, which may relate to the genesis of CSCC. Our results suggested that epigenetic modifications are responsible for aberrant expression of the hRFT2 gene, and may help to understand mechanisms of cervical carcinogenesis.