• The Korean Journal of Emergency Medical Services
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• v.12 no.3
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• pp.71-86
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• 2008

Purpose : This study was to examine the influence of job stress of 119 Emergency Medical Technician on fatigue and job satisfaction. Methods : Subjects of this study were 228 Emergency Medical Technicians who worked at 119 Safety Center of total 92 fire stations in 12 cities of Gyeonggido including U, N, H, B, A, S, Y, G, G, G, P and I and the period of data collection was from July 11 to Sep. 10, 2007. Collected data were analyzed with SPSS 14.0 version. Results : Conclusions of this study were as follows. 1) Factor related to organization among job stress factors had the greatest influence on fatigue (B = 0.334, p < 0.01), followed by crisis situation factor (B = 0.2042, p < 0.01), inappropriate treatment factor (B = 0.174, p < 0.05), role conflict factor as special job (B = 0.109, p < 0.05) and professional knowledge and technique factor (B = 0.109, p < 0.05), and the influence of job stress factors on fatigue was explained as $R^2=0.340$. 2) Mental burden factor of job stress factors had the greatest influence on job satisfaction (B = -0.606, p < 0.01), followed by organization factor (B = -0.473, p < 0.01) and interpersonal relation and conflict factor (B = -0.339, p < 0.01), and the influence of job stress factor on fatigue was explained as $R^2=0.308$. Conclusions : Job stress of 119 rescuer is increasing continuously and such an accumulated stress lowers the job efficiency. In order to reduce, job stress, it is important to extend(${\rightarrow}$ have) proper self-development, maintain close relationships and mutual correlations among members of organization in personal dimension and to solve the role conflicts, define the organizational roles and simplify excessive job description into concrete work.

• Journal of Animal Science and Technology
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• v.61 no.2
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• pp.61-68
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• 2019

The hG-CSF (human Granulocyte Colony-Stimulating Factor) is a growth and stimulation factor capable of inducing the proliferation of bone marrow cells, several types of leukocytes, among other hematopoietic tissue cells. hG-CSF is used in used to treat anomalies that reder a small number of circulating white blood cells, which may compromise the immune defenses of the affected person. For these reasons, the production of hG-CSF in a bioreactor system using the mammary gland of genetic modified animals is a possibility of adding value to the bovine genetic material and reducing the costs of hG-CSF production in pharmaceutical industry. In this study, we aimed the production of transgenic hG-CSF bovine through the lipofection of bovine primary fibroblasts with an hG-CSF expression cassette and cloning these fibroblasts by the somatic cell nuclear transfer (SCNT) technique. The bovine fibroblasts transfected with the hG-CSF cassette presented a stable insertion of this construct into their genome and were efficiently synchronized to G0/G1 cell cycle stage. The transgenic fibroblasts were cloned by SCNT and produced 103 transferred embryos and 2 pregnancies, one of which reached 7 months of gestation.

• Biomolecules & Therapeutics
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• v.2 no.3
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• pp.260-269
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• 1994

This study was performed to determine the toxic effect of DA-3030(granulocyte-colony stimulating factor, G-CSF) in beagle dogs. DA-3030(G-CSF) was injected intravenously at doses of 115 $\mu\textrm{g}$/kg/day, 11.5 $\mu\textrm{g}$/kg/day and 1.15 $\mu\textrm{g}$/kg/day seven days per week for 28 days. After completion of the treatments, the dog were necropsied. The number of dead animal was zero in all groups. No specific clinical sign was found, either. In hematological results, WBC was significantly increased dose-dependently in treated groups. In histopathological findings, megakaryocyte and rubricyte were found in the liver and spleen at the dose of 115 $\mu\textrm{g}$/kg/day. Therefore, we could find the extramedullary hematopoiesis was increased. Megaka yocyte and rubricyte were increased in bone marrow, too. In conclusion, those signs were estimated the pharmacological effect of DA-3030(G-CSF). According to the results, non toxic dose of DA-3030(G-CSF) was higher than 115 $\mu\textrm{g}$/kg/day.

• Korea-Australia Rheology Journal
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• v.20 no.4
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• pp.221-226
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• 2008

The purpose of this study was to compare rheological properties of six dental interocclusal recording materials and to investigate the effect of temperature on the rheological properties during setting. Five polyvinylsiloxane materials and one polyether material were investigated in this study. The storage modulus (G') and the loss factor ($tan{\delta}$) were measured from 30s after mixing during setting, using the universal dynamic spectrometer. Viscoelastic properties were evaluated by means of G' and $tan{\delta}$ from 5 repeats at $21^{\circ}C$ and $33^{\circ}C$. Individual changes during setting were also evaluated. All data were statistically analyzed using one-way ANOVA and multiple comparison $Scheff{\acute{e}}$ test at the significance level of 0.05. The mean of G was checked at $t_{set}$ (the setting time provided from manufacturer) and $t_{300}$ (the end of experimental time) and the mean of $tan{\delta}$ was checked at to and $t_{set}$. Whereas the increase of the G' value showed generally exponential changes at $21^{\circ}C$, the change of the G' value at $33^{\circ}C$ displayed sigmoidal curves during setting. The change of loss factor $tan{\delta}$ during setting varied. Within the limitations of this study, dental interocclusal recording materials had different viscoelastic properties and most of the materials showed different fluidity at $21^{\circ}C$ and $33^{\circ}C$.

• Korean journal of food and cookery science
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• v.22 no.6 s.96
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• pp.855-863
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• 2006

The purpose of this study was to provide the basic information on the functional cookies with pumpkin and ginseng powder. Pumpkin cookies with ginseng powder were prepared at various additions adding (0 g, 3 g, 6 g, 9 g). With increasing As the addition of ginseng powder additionincreased, there were significant decrease in the water contents and crude protein were significantly decreased (p<0.05). The spread factor and Hunter color's L, a, and b values in th dough and cookies were significantly decreased with increasing as the addition level of ginseng powder content was increased(p<0.05). Hardness measured using rheometer and onset, peak, and closing temperatures and gelatinization enthalpy using DSC showed significantly the higher values with increasing more additions of ginseng powder content. In sensory evaluation, surface cracking, and pumpkin flavor were decreased with lower levels of ginseng powder. Ginseng flavor and hardness were increased with lower levels of ginseng powder Overall desirability of cookies with addition of 3 g added ginseng powder was the highest of the four samplesproduction. Surface crakcracking washadhighly positively correlated with spread factor, a (redness) (p<0.01)and but negatively correlated with carbohydrate content (p<0.05). Ginseng aroma showed negative correlation to the water contents, crude protein, spread factor, and Hunter's a (redness) and b (yellowness) values (p<0.05).

• Proceedings of the Safety Management and Science Conference
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• 2010.11a
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• pp.113-129
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• 2010

Proper pre-safety training is one of core mandatory in order to reduce injuries at the construction fields. In this study, we investigate the employee's opinions of safety training, safety recognition, the actual conditions of safety through survey. As a result of study, incident factors are roughly divided into technical factor, managerial factor, and educational factor. We confirmed that the educational factor led by deficiency of safety awareness and knowledge was the major factor. It needs to be resettled or revised properly to match with the incident trends and the safety training curriculum should also be improved and drastically revised with special quality. The managerial workers' safety recognition is lower than the site ones regarding the correct understanding of the importance of the training.. It suggest that the education method and system in construction fields might have fundamental problems.

• Biomedical Science Letters
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• v.20 no.2
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• pp.56-61
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• 2014

This study attempted to confirm the antioxidative potential of luteolin against tert-butyl hydroperoxide (t-BHP) induced oxidative damage and to investigate its molecular mechanism related to glutathione (GSH)-dependent enzymes in HepG2 cells. Treatment with luteolin resulted in attenuation of t-BHP induced generation of reactive oxygen species (ROS) and oxidative stress-mediated cell death. In addition, accelerated expression of GSH-dependent antioxidative enzymes, glutathione peroxidase (GPx) and glutathione reductase (GR), and heme oxygenase (HO)-1, as well as strengthened GSH content was induced by treatment with luteolin, which was in accordance with increased nuclear translocation of nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), a transcription factor for phase 2 enzymes, in a dose-dependent manner. These results suggest that the cytoprotective potential of luteolin against oxidative damage can be attributed to fortified GSH-mediated antioxidative pathway and HO-1 expression through regulation of Nrf2 in HepG2 cells.

• Journal of Life Science
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• v.20 no.11
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• pp.1577-1581
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• 2010

Granulocyte colony stimulating factor (G-CSF) is a hematopoietic cytokine that stimulates bone marrow cells to proliferate and differentiate into granulocytes. G-CSF is approved and used for therapeutic purposes. The endoplasmic reticulum (ER) signal peptide of hG-CSF was replaced with silkworm-specific signal peptides to express and efficiently secrete recombinant hG-CSF by silkworm cells. Plasmids that contain cDNAs for hG-CSF and hG-CSF fused with silkworm- specific signal peptides of prophenoloxidase activating enzyme (PPAE), protein disulfide isomerase (PDI), and bombyxin (BX) were constructed. The G-CSF protein was expressed in insect cell line BM5 and was detected by western blot analysis. The cells transfected with plasmids containing rhG-CSF genes with silkworm-specific signal sequences released mature rhG-CSF protein more efficiently than the cells transfected with pG-CSF, the plasmid containing human G-CSF gene, including its own signal sequence. The production of hG-CSF reached maximal level at four days post-transfection and remained at a high level until 7 days post-transfection. These data demonstrate that the modification of the human G-CSF mimic to insect proteins synthesized in ER greatly improves the production of the protein.

• Journal of Environmental Health Sciences
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• v.33 no.3
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• pp.217-226
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• 2007

The purpose of this study is to estimate the emission factor of $non-CO_2$ global warming gases such as $N_2O$ and $CH_4$ by measuring concentrations from stacks of waste incinerators and cement production plants. Based on the established monitoring methods, $N_2O$ concentration measured from stacks in incinerator were between 0.62 and $40.60\;ppm_v$ (ave. $11.50\;ppm_v$). The concentration of $N_2O$ was dependent on the incinerator types. However, the concentrations of $CH_4$ gas were between 2.65 and $5.68\;ppm_v$ (ave. $4.22\;ppm_v$), and did not show the dependency on the incinerator types. In the cement production plant, the concentration ranges of $N_2O$ from the stack were from 6.90 to $10.80\;ppm_v$ (ave. $8.60\;ppm_v$), and $CH_4$ were between 1.80 and $2.20\;ppm_v$ (ave. $2.60\;ppm_v$). Using measured concentrations, the emission amounts of $N_2O$ and $CH_4$ from stacks per year were calculated. The results were is 4.2 ton $N_2O/yr$ in the incinerators, and 53.7 ton $N_2O/yr$ in the cement facilities. The big difference is from the flow rate of flue gas in the cement facilities compared to the incinerators. By the same reason, the $CH_4$ emission amounts in cement plant and incinerator was found to be 339 ton $CO_2/yr$ and 34.1 ton $CO_2/yr$, respectively. Finally, the emission factor of $N_2O$ in the incinerators were calculated using the measured concentration and the amount of incinerated wastes, and was $42.5\sim799.1\;g/ton$ in kiln and stoker type, $11.9\sim79.9\;g/ton$ in stoker type, 90.1 ton/g in rotary kiln type, 174.9 g/ton in fluidized bed type, and 63.8 g/ton in grate type, respectively. Also, the emission factors of $CH_4$ were found to 65.2-91.3 g/ton in kiln/stoker type, 73.9-122 g/ton in stoker type, 109.5 g/ton rotary kiln, and 26.1 g/ton in fluidized bed type. This result indicates that the emission factor in incinerators is strongly dependent on the incinerator types, and matched with result of IPCC (International Panel on Climate Change) guideline.

• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.321-326
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• 2000

To examine the feasibility of the long-term production of the human granulocyte colony stimulating factor (hG-CSF) using the $_{L}-arabinose$ promoter system of Escherichia coli, flask relay culture and cyclic fed-batch culture were performed. In the flask relay culture, it was found that the pismid was maintained stably up to about 170 generations in an uninduced condition, whereby the cells could also maintain the capability of expressing hG-CSF expression were maintained stably up to at least 100 generations. In contrast, in the cyclid fed-batch culture, segregational plasmid instability was observed within about 4 generations after induction, even though the cell growth and hG-CSF production reached their maximum balues, 78.0 g/l of dry cell weight and 7.0 g/l of hG-CSF, respectively. It would appear that, when compared to the flask relay culture, the high-cell density and high-level expression of hG-CSF in the cyclic fed-batch cultrure led to the segregational plasmid instability; in other words, a severe metabolic burden existe on the cells due to the high-level expression of hG-CSF. Accordingly, based on these long-term cultures, the segregational and structural plasmid instability was observed and a strategy to overcome such problems could be designed.