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Regulation of Nrf2 Mediated Phase II Enzymes by Luteolin in human Hepatocyte  

Park, Chung Mu (Department of Clinical Laboratory Science, Dong-Eui University)
Publication Information
Biomedical Science Letters / v.20, no.2, 2014 , pp. 56-61 More about this Journal
Abstract
This study attempted to confirm the antioxidative potential of luteolin against tert-butyl hydroperoxide (t-BHP) induced oxidative damage and to investigate its molecular mechanism related to glutathione (GSH)-dependent enzymes in HepG2 cells. Treatment with luteolin resulted in attenuation of t-BHP induced generation of reactive oxygen species (ROS) and oxidative stress-mediated cell death. In addition, accelerated expression of GSH-dependent antioxidative enzymes, glutathione peroxidase (GPx) and glutathione reductase (GR), and heme oxygenase (HO)-1, as well as strengthened GSH content was induced by treatment with luteolin, which was in accordance with increased nuclear translocation of nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), a transcription factor for phase 2 enzymes, in a dose-dependent manner. These results suggest that the cytoprotective potential of luteolin against oxidative damage can be attributed to fortified GSH-mediated antioxidative pathway and HO-1 expression through regulation of Nrf2 in HepG2 cells.
Keywords
Luteolin; Oxidative stress; Glutathione; Heme oxygenase-1; Nuclear factor-erythroid 2 p45-related factor 2;
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