• Title/Summary/Keyword: G+C rich

Search Result 290, Processing Time 0.021 seconds

Food Sources of Vitamin A and Vitamin C (비타민 A와 비타민 C의 급원식품 선정)

  • 김영남
    • Journal of Korean Home Economics Education Association
    • /
    • v.13 no.2
    • /
    • pp.1-14
    • /
    • 2001
  • The purpose of this study was to find out and advocate the intake of vitamin A and C rich foods in Korean people. Forty kinds of vitamin A and C rich foods were selected by the vitamin quantity in 100g edible portion. in single serving size. and by the 1997 national food supply data. The results were summarized as follows. 1. The vitamin A rich foods 1) The food sources of vitamin A presented in the middle and high school home economics textbooks were liver. egg/egg yolk. milk/dairy products. and green and yellow vegetables. etc. 2) The vitamin A rich foods by 100g edible portion ere in order of red pepper(dried). laver(dried). carrot. meat edible viscera. eel. etc. And the vitamin A rich foods by the vitamin A content in single serving size were in order of carrot. eel. meat edible viscera. water shield. red pepper(dried). etc. 3) The vitamin A suppling foods according to the 1997 national food supply data were in order of red pepper(dried). meat edible viscera. laver. carrot. etc. The green and yellow vegetables. fish and shellfish. and seaweeds were the most important sources of vitamin A in Korean. 2. The vitamin C rich foods 1) The food sources of vitamin C presented in the textbooks of middle and high school were strawberry. citrus fruits. and vegetables such as spinach. chinese cabbage. radish. crown daisy. etc. 2) The vitamin C rich foods on the basis of the vitamin C content in 100g edible portion were in order of sweet pepper. goose berry. citron. strawberry. water shield. etc. And the vitamin C rich foods by the quantity in single serving size were in order of strawberry. goose berry. citron. sweet pepper. lemon. etc. 3) The vitamin C suppling foods according to the 1997 national food supply data were in order of chinese cabbage. radish. citrus fruits. strawberry. etc. Not only vegetables and fruits but also seaweeds like dried laver and sea mustard were the most important source of vitamin C in korean.

  • PDF

Effects of Denaturants on the Conditions of Polymerase Chain Reactions with G+C-rich Primers (G+C 함량이 높은 Primer를 사용하는 중합효소 연쇄반응에서 변성제가 미치는 영향)

  • 김종배;안준환;엄용빈;김영미
    • Biomedical Science Letters
    • /
    • v.2 no.2
    • /
    • pp.241-247
    • /
    • 1996
  • Poor yields of amplified DNAs could be resulted in polymerase chain reaction(PCR) processes with G+C-rich DNA primers because of their high $T_m$ values. To maximize the yields of amplification in PCR processes with G+C-rich primers, we compared the yields of amplified DNA fragments according to the concentrations of specific denaturants added to the reaction mixture of PCR system. With addition of the mixture of 2.5% glycerol and 1.25% formamide, or 2.5% dimethyl sulfoxide to the reaction cocktail, respectively, remarkable increases in the yields of amplified DNA fragments were not observed in the PCR systems with G+C-low primers of Lyl chromosomal gene from Borrelia burgdorferi but observed in the PCR system with G+C- ich primers of Is900 gene from Mycobacterium parahberculosis. Although we were not practically able to discriminate the yields of PCR DNAs according to the concentrations used in this study, addition of the mixture of 5% glycerol and 2.5% formamide, or 5% DMSO tended to increase the production of extra bands.

  • PDF

ALLOY STRUCTURE AND ANODIC FILM GROWTH ON RAPIDLY SOLIDIFIED AL-SI-BASED ALLOYS

  • Kim, H.S.;Thompson, G.E.;Wood, G.C.;Wright, I.G.;Maringer, R.E.
    • Journal of the Korean institute of surface engineering
    • /
    • v.17 no.2
    • /
    • pp.29-40
    • /
    • 1984
  • The structure of rapidly solidified Al-Si-based alloys and its relationship to subsequent anodic film growth in near neutral and acid solutions have been investigated. Solidification of the alloys proceeds via pre-dendritic nuclei, associated with rugosity of the casting surface, from which cellular-type growth, comprised of aluminium-rich material surrounded by silicon-containing material, emanates. Observation of ultramicrotomed sections of the alloys and their anodic films reveals the local oxidation of the silicon-rich phase and its incorporation into the anodic alumina film, formed in near neutral solutions. Such incorporation occurs but resultant isolation of the silicon-rich phase is not possible for anodizing in phosphoric acid, and a three-dimensional network of the oxidized silicon-containing phase, with continuing development of porous anodic alumina, is observed.

  • PDF

AtbZIP16 and AtbZIP68, two new members of GBFs, can interact with other G group bZIPs in Arabidopsis thaliana

  • Shen, Huaishun;Cao, Kaiming;Wang, Xiping
    • BMB Reports
    • /
    • v.41 no.2
    • /
    • pp.132-138
    • /
    • 2008
  • AtbZIP16 and AtbZIP68 are two putative G group bZIP transcription factors in Arabidopsis thaliana, the other three members of G group bZIPs are GBF1-3 which can bind G-box. Members of G group have conservative protein structure: highly homological basic region and a proline-rich domain in the N-terminal region. Here, we report that AtbZIP16 and AtbZIP68 could bind cis elements with ACGT core, such as G-box, Hex, C-box and As-1, but with different binding affinities which from high to low were G-box > Hex > C-box > As-1; AtbZIP16 and AtbZIP68 could form homodimer and form heterodimer with other members of G group; N-terminal proline rich domain of AtbZIP16 had transactivation activity in yeast cells while that of AtbZIP68 did not; AtbZIP16 and AtbZIP68 GFP fusion protein localized in the nucleus of onion epidermal cells. These results indicated that AtbZIP16 and AtbZIP68 were two new members of GBFs. In Arabidopsis, AtbZIP16 and AtbZIP68 may also participate in light-responsive process in which GBF1-3 are involved.

Study on Pretreatment of Giant Miscanthus Using Ionic Liquid and Structural Change of Lignin (이온성 액체에 의한 거대억새 전처리 특성 및 리그닌의 구조적 변화 관찰)

  • Park, Shin Young;Hwang, Hyewon;Jang, SooKyeong;Choi, In Gyu;Choi, JoonWeon
    • Journal of the Korean Wood Science and Technology
    • /
    • v.43 no.3
    • /
    • pp.344-354
    • /
    • 2015
  • To investigate the effects of ionic liquid pretreatment on biomass, giant miscanthus was treated with 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]) and 1-butyl-3-methylimidazolium acetate ([Bmim][OAc]) at three temperature conditions ($90^{\circ}C$, $110^{\circ}C$, and $130^{\circ}C$). As temperature condition increased, yield of the cellulose-rich product (CP) was reduced from 87.2% to 67.6%, while yield of the ionic liquid lignin (ILL) increased from 2.2% to 9.9%. Compared to the ILL, CP had lower carbon contents and higher oxygen contents. Enzymatic hydrolysis of CPs showed that conversion ratio of CP treated with [Emim][OAc] at $110^{\circ}C$ was 56.7%, the highest digestibility. Thermogravimetric analysis indicated that the maximum degradation rate decreased as temperature condition increased. In addition, maximum degradation temperature of ILL treated with [Emim][OAc] ranged from 274 to $279^{\circ}C$ which was lower than that of ILL treated with [Bmim][OAc]. Analytical date for ${\beta}$-O-4 linkage frequency in the ILL revealed that ${\beta}$-O-4 linkage frequency in the ILL decreased as the temperature rose. Furthermore, the highest S/G ratio of the ILL was determined to ca. 1.2 obtained from [Bmim][OAc] treatment at $110^{\circ}C$.

Improved PCR Amplification of Human DRD4 Polymorphic 48-bp Repeats : PCR of Digested Template for GC-rich Targets (수용체의 다형성 48-hp반복배열의 PCR을 위한 효과적 방법 : 제한효소로 절단된 GC-rich목표배열의 중합효소연쇄반응)

  • Sheen, Joon-Ho;Kim, Young-Tae;Lee, Min-Soo
    • Korean Journal of Biological Psychiatry
    • /
    • v.2 no.2
    • /
    • pp.248-251
    • /
    • 1995
  • 정신분열병의 약물치료에서 중요한 역할을 하는 도파민의 기전은 지금까지 5종류의 수용체들이 발견되고 클론되면서, 새롭고 보다 근본적인 유전학적 접근을 통해 규명될 수 있게 되었다. 특히, 도파민 수용체 D4 (DRD4)는 막단백질의 세포질쪽 세번째굴곡에 48-bp반복 다형성배열을 가지고있다. 이러한 다형성 반복배열이 신호전달에 참여할 가능성이 높은 막단백질의 세포질쪽 굴곡에 있다는것은, 각 개인의 항정신병약물에 대한 민감성의 차이를 포함하여 정신분열병에 대한 개개인의 유전적 차이를 진단할 수 있는 가능성을 제시한다. 이러한 가능성을 검증하기 위하여 주로 손쉽고 빠른 중합효소연쇄반응(PCR)을 사용하여 DRD4의 아형들을 분류하게 되는데, DRD4의 PCR은 그 반복배열과 그 주변배열의 높은 GC함량(78% G+C) 때문에 일반적인 PCR 방법을 변형시켜 사용해야한다. DRD4의 아형을 분류하기 위해 변형된 PCR은 통상적으로 7-deaza dGTP와 10% DMSO를 사용하게된다. 이러한 DRD4 PCR은 대부분의 경우 성공하지만, 항상 모든 시료에서 PCR이 성공되는것은 아니었으며 반복적으로 시도하여 증폭시킬 수 있었다. 이러한 어려움은 대부분이 template DNA에 문제가 있을것으로 의심되며 DNA정제 또는 template DNA를 제한효소로 적절하게 무작위절단하여 성공율을 높일 수 있었다.

  • PDF

Thermodynamic Analysis of Intergranular Additives in Sintered Nd-Fe-B Magnet

  • Cui, X.G.;Wang, X.H.;Cui, C.Y.;Yin, G.C.;Xia, C.D.;Cheng, X.N.;Xu, X.J.
    • Journal of Magnetics
    • /
    • v.22 no.2
    • /
    • pp.244-249
    • /
    • 2017
  • To get deeper insight into the effect of intergranular additives in sintered Nd-Fe-B magnet and consequently improve the properties better, the interaction between additives (oxide, nitride, and carbide) and Nd-rich phase in the temperature range of 298.15-1400 K was analyzed thermodynamically. It can be found that the oxide additives became less stable than nitrides and carbides. Except for calcium oxide, almost all oxides could react with Nd from Nd-rich phase. To be different from oxide additives, the mechanism of nitrides and carbides was defined with various elements, either reaction with Nd from Nd-rich phase or not. The two different mechanisms would show different effects on the microstructure and hence properties of magnet. The thermodynamic analysis had a better agreement with the experimental information.

A Study on the Structure Properties of Amorphous $As_{40}Se_{50-x}S_{x}Ge_{10}$ Thin Film (비정질 $As_{40}Se_{50-x}S_{x}Ge_{10}$ 박막의 구조특성에 관한 연구)

  • 김종빈;정홍배
    • Journal of the Korean Institute of Telematics and Electronics A
    • /
    • v.29A no.11
    • /
    • pp.106-112
    • /
    • 1992
  • In this paper, we analyze the structure characteristics of $As_{40}Se_{50-x}S_{x}Ge_{10}$ system bulk and thin films. As the results of XRD patterns, it identified amorphous state. In order to find the glass transition temperature($T_g$), crystallization($T_c$) and melting point ($T_m$)of bulk sample, it ascertained that TS1gT is 238$^{\circ}C$ in $As_{40}Se_{15}S_{35}Ge_{10}$, and 231$^{\circ}C$ in $As_{40}Se_{25}S_{25}Ge_{10}$ & $As_{40}Se_{50}Ge_{10}$ following the thernal analysis by DSC, DTA, & TGA method. Also it was confirmed the phase seperation of continuous phase and dispertion phase by the optical texture of polarizing microscope and $T_g$ near 20$0^{\circ}C$ in thin film. Therefore, it was found that it occurs the phase seperation of Ge-rich dontinuous phase and Se-rich dispersion phase following the EDS analysis of thin film and the surface SEM photograph.

  • PDF

Effects of Dietary Fatty Acid and Protein Sources on Serum Protein Profiles and Liver Functional Enzyme Activities in Rats with DMBA-Induced Mammary Tumors

  • Lee, Eun-Kyung;Kim, Hyun-Sook;Lee, Sang-Sun
    • Nutritional Sciences
    • /
    • v.5 no.2
    • /
    • pp.53-59
    • /
    • 2002
  • This study was conducted to examine the effects of dietary sources of fatty acids and protein on serum protein profiles, hepatic functional enzyme activities, mammary tumor incidence and tumor weight in 7, 12-dimethylbenz($\alpha$)anthracene (DMBA)-treated rats. The sources of dietary fatty acids were 18n6 (rich in linoleic acid), 18n3 (rich in linolenic acid) and 22n3 (rich in DHA) : sources of dietary protein were casein (C) and soy protein isolate (S). mammary tumors (MTs) were chemically induced by DMBA (9 mg/100 g body weight) which was gastrically intubated at 7 weeks of age. Each experimental diet was given for the following 25 weeks. Casein-fed rats (group C) exhibited significantly higher levels of weight gain and FER (food efficiency ratio) than did group S. Group C showed higher levels of serum protein and globulin, and higher albumin/globulin (A/G) ratios than group S. Liver functional enzyme activities (GOT, GPT, ALP, LDH, $\gamma$-GT) and LDH/GOT ratios were not influenced by dietary protein. GPT activity was lower in the group given 18n3, and ALP activity was lower in the group given 18n6. The incidence and total number of MTs appeared to be lower in the group given 22n3 than in the group given 18n3 or 18n6, even though the average weight of MTs was highest in the group given 22n3, The average weight of MTs was higher in the C group than in the S group. MT incidence had a positive correlation with LDH activity and LDH/GOT ratio. The average weight of MTs had a negative correlation with serum albumin levels and A/G ratios, and a positive correlation with ALP activity. This research suggests that the measurement of serum protein profiles and liver functional enzyme activities may be utilized to monitor the development of mammary tumors.

Evaluation of Embrittlement in Isochronal Aged Fe-Cr Alloys by Magnetic Hysteresis Loop Technique

  • Mohapatra, J.N.;Kamada, Y.;Kikuchi, H.;Kobayashi, S.;Echigoya, J.;Park, D.G.;Cheong, Y.M.
    • Journal of Magnetics
    • /
    • v.16 no.2
    • /
    • pp.173-176
    • /
    • 2011
  • Fe-Cr alloys with different Cr contents were prepared by an arc melting technique. The alloys were isochronally aged in the range from $400^{\circ}C$ to $900^{\circ}C$ with $50^{\circ}C$ steps with a holding time of 100 hours. The ageing produced embrittlement in the alloys due to either the formation of a Cr-rich ${\alpha}'$ phase or a $\sigma$ phase at high temperatures. Magnetic Hysteresis Loop (MHL) and Micro-Vickers hardness were measured at each step to correlate the magnetic and mechanical properties. Coercivity and hardness of the alloys were increased and remanence decreased up to 500-$550^{\circ}C$ due to formation of a Cr-rich ${\alpha}'$ phase. Beyond 500-$550^{\circ}C$ range, the coercivity and hardness decreased and remanence increased due to the coarsening or dissolution of the Cr-rich ${\alpha}'$ phase. In the Fe-48% Cr alloy, formation of the $\sigma$ phase at $700^{\circ}C$ reduced the maximum induction of the alloy significantly.