• Title/Summary/Keyword: G+C mol%

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Studies on the Clostridium bovis sp. nov., the predominant species isolated from the feces of Holstein cattle (홀스타인 젖소의 분변에서 우세균종으로 분리되는 새로운 Clostridium bovis 에 관한 연구)

  • Lee, Wan-kyu
    • Korean Journal of Veterinary Research
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    • v.34 no.1
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    • pp.99-105
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    • 1994
  • Clotridium bovis sp. nov, is described on the basis of 5 strains isolated from the feces of Holstein cattle. The isolate are gram-positive, motile, strict anaerobic spore forming rods. They differ from all the validly described related species of the genus Clostridium in carbohydrate fermentation pattern, G+C mol% and DNA homologies. Acid is produced from arabinose, xylose, glucose, mannose, fructose, galactose, sucrose, maltose, cellobiose, lactose, trehalose, melibiose, raffinose, inulin and salicin. Major end products in PYFG broth are large amounts of butyric acid and lactic acid, and trace amounts of acetic and succinic acids. The G+C mol% of DNA from the type strain is 26 mol%. The type strain of Clostridium bovis is Catt $66^T$ strain.

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Chemosystematic and Phenotypic Characterization of Gram-positive Bacteria from Coastal Seawater, Korea (한국 근해에서 분리한 그람양성 세균의 화학 분류학적 및 표현형적 특성)

  • 전정훈;박진숙
    • Korean Journal of Microbiology
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    • v.36 no.3
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    • pp.167-172
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    • 2000
  • Twenty-five halotolerant gram-positlve bacteria were isolated from the coastal seawater 01 Cheju Island and Incheon J&yakdo Chemosystematic and phenotypic characteristics were used to iuvestigate the taxonomic position of these bacteria. According to their chemosystematic characteristics, the twenty-tive isolates were divided into 4 groups. Group 1 bacteria possesed 40.1 to 49.9 inol% m DNA G+C content, menaquinone-7 as a major quinone, and meso-Alpm as a diamino acid of peptidoglycan. Group 1 tam were identified as Bacilluspumilis, Bacillus lichenifbrrizis, Bacillus megaterium, Bncill~rsubtilis. Group 2 bacteria possessed 63.9 to 66.4 mol% and MK-8. They were all in the genus Arth~obaclm-. Group 3 bacteria possessed 31.0 to 37.6 mol% and MK-7. They were identified as Staphylococcus haeniol.viicvs, Siaph~~lococc~is sapropl~j~ticns, and Siaphylococcus intermediru.. Group 4 bacterium possessed 72.0mol% and MK-8 and was identified as ~Lficrococcus ltdtezm. Bacillus species accounted for 68% of h e total isolates.

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Output Enhancement of Coaxial Flashlamp Pumped Rhodamine 6G Dye Laser by Energy Transfer (에너지 전환에 의한 동축 섬광관 펌핑 Rhodamine 6G 색소 레이저의 출력 증가)

  • 장우권
    • Korean Journal of Optics and Photonics
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    • v.4 no.2
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    • pp.195-199
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    • 1993
  • The output energy of Rh-6G dye laser was enhanced by the energy transfer in the mixture of Rh-6G and C-545. The laser was pumped by coaxial flashlamp filled argon gas. The optimum concentration of Rh-6G was $10_-4mol/l$ without mixing. The output energy was enhanced about 70 % at 0.4 % C-545 mixture with respect to the concentration of Rh-6G. The peak output power and the output energy were 27 kW and 50 mJ at the pumping energy of 346 J.

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Analysis of the Seasonal Change of the Proximate Composition and Taste Components in the Conger Eels (Conger myriaster) (붕장어(Conger myriaster)의 일반성분 및 맛 성분의 계절별 변화 분석)

  • Ryu, Keun-Young;Shim, Sung-Lye;Kim, Won;Jung, Min-Seok;Hwang, In-Min;Kim, Jun-Hyeong;Hong, Cheul-Hee;Jung, Chan-Hee;Kim, Kyong-Su
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.8
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    • pp.1069-1075
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    • 2009
  • Conger eel (Conger myriaster) is used as a well-being food in the foodservice industry in Korea. We analyzed not only the proximate composition but also the taste components affecting conger eel, which are fatty acids, nucleotides, amino acids, etc. Concerning the composition, the crude lipid was the lowest in summer at 3.2%, which is considered due to spawning period. The major fatty acids were $C_{16:0},\;C_{16:1},\;C_{18:0},\;C_{18:1},\;C_{20:5},\;and\;C_{22:6}$. The $C_{18:1}$ content was the highest among the fatty acids and the content varied between 36.76 and 45.11% by season. Seasonal change in the content of poly-unsaturated fatty acids was increased from spring to winter in conger eel. Among the nucleotides, the contents of IMP (3.617$\sim$5.524 $\mu$mol/g) and Hx (0.913$\sim$2.238 $\mu$mol/g), which is closely related to taste, and the concentrations of IMP and Hx were the highest (7.219 $\mu$mol/g) in winter, and HxR (0.625$\sim$1.652 $\mu$mol/g) was higher than ATP (0.058$\sim$0.083 $\mu$mol/g), ADP (0.145$\sim$0.161 $\mu$mol/g), and AMP (0.166$\sim$0.179 $\mu$mol/g). In conger eels, the major total and free amino acids were glutamic acid (14,178.7$\sim$7,802.6 mg%), aspartic acid (4,669.2$\sim$8,259.0 mg%), lysine (4,198.3$\sim$7,540.8 mg%), leucine (3,843.6$\sim$6,782.1 mg%), and histidine (199.6$\sim$644.4 mg%), glycine (94.8$\sim$152.2 mg%), alanine (35.3$\sim$71.2 mg%), glutamic acid (44.1$\sim$70.6 mg%), respectively, but the concentration of amino acids was different by season. The content of free amino acids, which is related to the taste component, was detected as high in summer and winter at 1179.2 and 1,605.2 mg%, respectively.

Prevention of Discoloration and Storage Stability in Canned Ark Shell (새고막 통조림 변색방지 및 저장중 품질변화)

  • 배태진;김귀식
    • The Korean Journal of Food And Nutrition
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    • v.11 no.2
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    • pp.243-248
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    • 1998
  • Ark shell was known as shellfish that had hemoglobin as blood pigment and the action of mecidine, was consumed the great part of it as raw material, though it was produced about 13,000 M/T per year. Ark shell was processed the infinitesimal quantity as conned product, bout canned ark shell had problem that occurrenced discoloration after heat treatment during processing and storage. This discoloration mechanism during processing and storage was not cleared. This study was carried out to understand characteristics of the hemoglobin as blood pigment and carotenoid as meat pigment in ark shell and management of proper processing conditions for prevention of oxidation and discoloration by thermal treatment. When treated by digestion of 0.1% BHA, 0.1% Tenox-II, 0.5% Na2EDTA, 0.05% NDGA and 3% salt soln., 0.1% BHA solution was most suitable for stability of carotenoid that the retention ratio of carotenoids were 63.1% after heating to 116$^{\circ}C$ for 120 minutes. In preparation of canned ark shell and storage at 37$\pm$1$^{\circ}C$ for 60 days, the chemical composition, pH and salinity ere stable. And contents of total carotenoid were decreased slightly from 0.83mg% to 0.727mg%. The viable cell count were 6.92$\times$103 cfu/ml at raw ark shell, after processed and storage were not detected. The predominant amino acids in the raw ark shell were glutamic acid(19.7%), arginine(16.0%), glycine(12.6%), alanine(12.2%) and aspartic acid(7.6%). When 60 days stored, the contents of amino acid were stable. And the predominant nuclotide and their related compounds in the raw ark shell were hypoxanthine(2.14$\mu$mol/g), IMP(1.94$\mu$mol/g) and ATP(0.87$\mu$mol/g), and storage at 37$\pm$1$^{\circ}C$ for 60 days, the quantity order were same as raw material.

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Synthesis and Curing Behaviors of Polyisoimide Oligomers with Ethynyl End Groups (Ethynyl 말단기를 갖는 Polyisoimide 올리고머의 합성 및 이들의 경화거동에 관한 연구)

  • Choi, Seok Woo;Kim, Bo Ock;Kim, Ji-Heung;Nam, Sung Woo;Jeon, Boong Soo;Kim, Young Jun
    • Polymer(Korea)
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    • v.38 no.6
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    • pp.774-781
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    • 2014
  • Acetylenic or phenylethynyl end-capped polyisoimide oligomers ($M_w$ 2500 g/mol, 5000 g/mol) based upon 4,4'-diamino diphenyl ether (4,4'-ODA)/4,4'-oxydiphthalic anhydride (ODPA) and 4,4'-ODA/3,3',4,4'-benzophenone tetracarboxylic acid dianhydride (BTDA) were synthesized by using 4-ethynylaniline (4-EA) or 4-phenylethynyl phthalic anhydride (4-PEPA) as an end capper. The incorporation of ethynyl groups were confirmed by FTIR spectroscopy. The isomerization temperature was influenced by molecular weight as well as the backbone structure of polyisoimides oligomers. Thus, polyisoimide oligomers with molecular weight of 2500 g/mol was found to be imidized at temperature $10^{\circ}C$ lower than that for the oligomers with molecular weight of 5000 g/mol. The crosslinking reaction of ethynyl groups occurred at a higher temperature than that for the isoimide/imide isomerization reaction. These two reactions were totally or partially overlapped on the DSC thermograms for the polyisoimide oligomer end-capped with 4-EA. Kinetics of thermal imidization and crosslinking reactions for the 4,4'-ODA/ODPA polyisoimide oligomers end-capped with 4-PEPA were investigated by performing dynamic DSC experiments at heating rate of $10^{\circ}C/min$. The activation energy and pre-exponential factors were 141 kJ/mol and $1.45{\times}10^{13}min^{-1}$ for the imidization reaction and 177 kJ/mol and $2.90{\times}10^{13}min^{-1}$ for the crosslinking reaction, respectively.

Kinetics Study for Wet Air Oxidation of Sewage Sludge (하수슬러지의 습식산화반응에 대한 동력학적 연구)

  • Ahn, Jae-Hwan
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.7
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    • pp.746-752
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    • 2005
  • In this study, the effect of reaction parameters including reaction temperature, time, and pressure on sludge degradation and conversion to intermediates such as organic acids were investigated at low critical wet air oxidation(LC-WAO) conditions. Degradation pathways and a modified kinetic model in LC-WAO were proposed and the kinetics model predictions were compared with experimental data under various conditions. Results in the batch experiments showed that reaction temperature directly affected the thermal hydrolysis reaction rather than oxidation reaction. The efficiencies of sludge degradation and organic acid formation increased with the increase of the reaction temperature and time. The removal of SS at $180^{\circ}C$, $200^{\circ}C$, $220^{\circ}C$ and $240^{\circ}C$ of reaction temperatures and 10 min of reaction time were 52.6%, 68.3%, 72.6%, and 74.4%, respectively, indicating that most organic suspended solids were liquified at early stage of reaction. At $180^{\circ}C$, $200^{\circ}C$, $220^{\circ}C$ and $240^{\circ}C$ of reaction temperatures and 40 min of reaction time, the amounts of organic acids formed from 1 g of sludge were 93.5 mg/g SS, 116.4 mg/g SS, 113.6 mg/g SS, and 123.8 mg/g SS, respectively, and the amounts of acetic acid from 1 g of sludge were 24.5 mg/g SS, 65.5 mg/g SS, 88.1 mg/g SS, and 121.5 mg/g SS, respectively. This suggested that the formation of sludge to organic acids as well as the conversion of organic acids to acetic acid increased with reaction temperature. Based on the experimental results, a modified kinetic model was suggested for the liquefaction reaction of sludge and the formation of organic acids. The kinetic model predicted an increase in kinetic parameters $k_1$ (liquefaction of organic compounds), $k_2$ (formation of organic acids to intermediate), $k_3$ (final degradation of intermediate), and $k_4$ (final degradation of organic acids) with reaction temperature. This indicated that the liquefaction of organic solid materials and the formation of organic acids increase according to reaction temperature. The calculated activation energy for reaction kinetic constants were 20.7 kJ/mol, 12.3 kJ/mol, 28.4 kJ/mol, and 54.4 kJ/mol, respectively, leading to a conclusion that not thermal hydrolysis but oxidation reaction is the rate-limiting step.

Synthesis of PLLA-block-PMMA Copolymer and Characteristics of Biaxially Oriented PLA Film Including the Same (PLLA-block-PMMA 공중합수지의 합성 및 이를 포함하는 PLA 이축연신 필름의 특성)

  • Kim, Moon-Sun;Lee, Sangeun
    • Applied Chemistry for Engineering
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    • v.26 no.3
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    • pp.251-258
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    • 2015
  • In the study, PLLA with 12,000 g/mol ($M_n$) and 14,000 g/mol ($M_w$) was synthesized from L-lactide, and used to synthesize PLLA-Br intermediate. PLLA-block-PMMA with 84,000 g/mol ($M_n$) and 126,000 g/mol ($M_w$) was finally synthesized from PLLA-Br intermediate. The glass transition temperature ($T_g$) and initial pyrolysis temperature of PLLA-block-PMMA are $95.5^{\circ}C$ and $289^{\circ}C$, respectively. The PLA film of $50{\pm}3{\mu}m$ thickness was prepared by blending PLA with 9 phr PLLA-block-PMMA followed by stretching biaxially at 3 times under $95^{\circ}C$, and annealing at $120^{\circ}C$ for 2 min. The light transmittance at 550 nm and tensile strength of the film are 88.5% and 44.5 MPa, respectively. To enhance the tensile strength of PLA film, it was required to keep the film more than 2 min at $120^{\circ}C$ during the annealing step after a biaxially orientation.

Studies on the general analysis and antioxidant component analysis of Chenopodium album var. centrorubrum and biochemical analysis of blood of mice administered C. album (명아주의 일반성분, 항산화활성 분석 및 흰쥐의 혈중 생화학적 분석에 관한 연구)

  • Han, Kyoung-Sik;Jung, Tae-Hwan;Shin, Kyung-Ok
    • Korean Journal of Food Science and Technology
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    • v.51 no.5
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    • pp.492-498
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    • 2019
  • The purpose of this study was to develop new food materials by analyzing nutritional components and antioxidant activities of Chenopodium album var. centrorubrum. The highest amount of mineral obtained in C. album was found to be $1.01{\pm}0.07mg$ per 100 g of iron. The total phenolic content in C. album was found to be 3.77~9.57 GAE (gallic acid equivalent) mg/g. The reducing activities of leaves and roots of C. album determined using FRAP (ferric-reducing antioxidant power) were higher in ethanol extracts than water extracts. The ABTS radical scavenging activities of leaves and roots of C. album were $204.29{\pm}4.98{\mu}mol/g$ and $106.96{\pm}2.81{\mu}mol/g$, respectively. The DPPH radical scavenging activity was high upon extraction using ethanol (roots $20.71{\pm}0.04{\mu}mol/g$, leaves $71.08{\pm}0.33{\mu}mol/g$). HDL-cholesterol was significantly higher in the high fat diet groups supplemented with C. album than the control groups (p<0.05). These results suggest that C. album can be used as a natural antioxidant and a functional dietary supplement.

Sintering phenomena and grain growth of ultra-fine spinel($MgAl_2O_4$);(II) (순수 스피넬($MgAl_2O_4$)의 입성장 및 소결현상)

  • 이형복;한영환
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.8 no.3
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    • pp.498-502
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    • 1998
  • Sintering phenomena at refractory temperature ranges, from $1400^{\circ}C$ to $1700^{\circ}C$, of the pure spinel $(MgAl_2O_4)$ are analysed and compared to the experimental data from other researchers in terms of grain size(G), density($\rho$), and activation energy(Q). The grain size and relative density relationships for the spinels present very similar trends. They exhibit two distinct regions, an intermediate sintering stage to about the 85~90% density level and what appears to be the final stage sintering region above that transition-density level. The activation energy in terms of the grain size (G) and density ($\rho$) is determined to be 670$\pm$48 (kJ/mol) in this spinel and about 590 kJ/mol for the overall temperature range in other's spinel. These values are close to other published data, 360 to 580 kJ/mol.

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