• 폴리머
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• 제33권1호
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• pp.26-32
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• 2009

골 결손 치료를 위해 생분해성 고분자인 PLGA에 골다공증 치료제인 이프리플라본(IP)을 함유한 미립구를 O/W 유화 용매 증발법으로 제조하였으며, 생체외 방출실험에서 IP 방출량은 HPLC로 분석하였다. SEM을 이용하여 미립구에 부착된 세포의 거동을 확인하였으며, IP가 세포에 미치는 독성평가는 CCK-8 분석방법을 이용하여 측정하였다. 골 형성 지표인 ALP 활성도를 측정하였으며, 배양된 BMSCS의 골세포로의 표현형을 확인하기 위하여 RT-PCR을 수행하였다. 방출결과에서 IP 방출은 거의 40일 이상으로 지속적이었으며, IP를 함유한 미립구에서의 세포의 부착, 성장 등이 잘 이루어짐을 확인하였고, ALP 활성도 및 RT-PCR 분석결과에서도 단독의 PLGA 미립구보다 IP를 함유한 미립구에서의 값이 더 높은 것을 확인할 수 있었다. 본 실험결과를 바탕으로 향후 서방성 제제화에 있어서 IP/PLGA 미립구를 응용하게 되면 국소지향성 골분화 주사용 지지체로서 중요한 역할을 할 것으로 보인다.

• Nuclear Engineering and Technology
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• 제56권4호
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• pp.1501-1512
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• 2024

Plasma disruption in tokamak experiments is a challenging issue that causes damage to the device. Reliable prediction methods are needed, but the lack of full understanding of plasma disruption limits the effectiveness of physics-driven methods. Data-driven methods based on supervised learning are commonly used, and they rely on labelled training data. However, manual labelling of disruption precursors is a time-consuming and challenging task, as some precursors are difficult to accurately identify. The mainstream labelling methods assume that the precursor onset occurs at a fixed time before disruption, which leads to mislabeled samples and suboptimal prediction performance. In this paper, we present disruption prediction methods based on anomaly detection to address these issues, demonstrating good prediction performance on J-TEXT and EAST. By evaluating precursor onset times using different anomaly detection algorithms, it is found that labelling methods can be improved since the onset times of different shots are not necessarily the same. The study optimizes precursor labelling using the onset times inferred by the anomaly detection predictor and test the optimized labels on supervised learning disruption predictors. The results on J-TEXT and EAST show that the models trained on the optimized labels outperform those trained on fixed onset time labels.

• Journal of Microbiology and Biotechnology
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• 제14권1호
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• pp.81-89
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• 2004

Tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and lymphotoxin-$\alpha$ (LT-$\alpha$, TNF-$\beta$) can initiate and perpetuate human diseases such as multiple sclerosis (MS), rheumatoid arthritis (RA), and insulin-dependent diabetes mellitus (IDDM). TNFs can be blocked by the use of soluble TNF receptors. However, since monomeric soluble receptors generally exhibit low affinity or function as agonists, the use of monomeric soluble receptors has been limited in the case of cytokines such as TNF-$\alpha$, TNF-$\alpha$, interleukin (IL)-1, IL-4, IL-6, and IL-13, which have adapted to a multi component receptor system. For these reasons, very high-affinity inhibitors were created for the purpose of a TNFs antagonist to bind the TNFR and trigger cellular signal by using the multistep polymerase chain reaction method. First, recombinant simple TNFR-Ig fusion proteins were constructed from the cDNA sequences encoding the extracellular domain of the human p55 TNFR (CD120a) and the human p75 TNFR (CD120b), which were linked to hinge and constant regions of human $IgG_1$ heavy chain, respectively using complementary primers (CP) encoding the complementary sequences. Then, concatameric TNFR-Ig fusion proteins were constructed using recombinant PCR and a complementary primer base of recombinant simple TNFR-Ig fusion proteins. For high level expression of recombinant fusion proteins, Chinese hamster ovary (CHO) cells were used with a retroviral expression system. The transfected cells produced the simple concatameric TNFR-Ig fusion proteins capable of binding TNF and inactivating it. These soluble versions of simple concantameric TNFR-Ig fusion proteins gave rise to multiple forms such as simple dimers and concatameric homodimers. Simple TNFR-1g fusion proteins were shown to have much more reduced TNF inhibitory activity than concatameric TNFR-Ig fusion proteins. Concatameric TNFR-Ig fusion proteins showed higher affinity than simple TNFR-Ig fusion proteins in a receptor inhibitor binding assay (RIBA). Additionally, concatameric TNFR-Ig fusion proteins were shown to have a progressive effect as a TNF inhibitor compared to the simple TNFR-Ig fusion proteins and conventional TNFR-Fc in cytotoxicity assays, and showed the same results for collagen induced arthritis (CIA) in mice in vivo.

• 대한원격탐사학회지
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• 제35권6_4호
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• pp.1363-1372
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• 2019

본 연구에서는 영상융합 기법을 적용하여 고해상 다분광 영상을 제작할 때 요구되는 융합 파라미터를 분석하고, 하나의 값으로 대표될 수 있는 융합 파라미터를 제시하고자 하였다. 이를 위하여, 한반도 영역을 촬영한 115장의 KOMPSAT-3A 영상을 대상으로 전정색 영상을 모사할 수 있는 선형회귀계수와, 융합영상 제작에 요구되는 융합계수를 분석하였으며, 대표 선형회귀계수와 대표 융합계수를 제시하였다. 대표 융합 파라미터를 사용하여 융합영상을 제작하였을 때, 각 융합영상 간 DN값의 차이가 최적의 융합 파라미터를 사용하였을 때보다 정량적으로 감소함을 확인하였다. 따라서 본 연구는 융합영상 제작에 반영되는 지역적 특성을 최소화할 수 있는 것으로, 융합영상을 하나의 제품 측면에서 제공할 때 활용 가치가 높다.

• 한국조리학회지
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• 제14권4호
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• pp.134-149
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• 2008

This thesis is aimed to analyze the importance, satisfaction, perception and intake frequency of fusion menu in order to develop the market segmentations and marketing strategies for useful information on the fusion menu and its improvement in the food industries. The findings of the study are as follows: First, the study revealed that diet menu(low fat, low-cal) and vegetarian menu items have more influence on females than males in regard to the importance of fusion menu when examining gender. Second, the study revealed that Bulgogi pizza, Bulgogi burger, cheese cutlet, cheese kimbab, sweet and sour pork items have great influence on customers in their 10s while green tea latte, rice burger, Bulgogi pizza, kimchi hamburger items have a high effect on customers in their 20s in regard to the perception of fusion menu when examining age. Finally, the study revealed that the taste of food, the cleanliness of vessels, food hygiene, the freshness of food, the quality of menu, the portions of food, the nutrition of food, the speed of food service, food material harmony, the temperature of food, the flavor of food, distinctions from existing food, environment-friendly organic agriculture food material items have maintaining the good performance of fusion menus. It also showed that various strategies for price of fusion menu should be made when examining the IPA analysis.

• Nuclear Engineering and Technology
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• 제38권8호
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• pp.793-802
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• 2006

The design concept of a bending magnet to be installed in the KSTAR NBI system is presented. It is the function of a bending magnet that removes unconverted ions from the main beam stream and produces an 8 MW, 120 keV deuterium neutral beam. In order to determine the proper size and shape of the bending magnet, a parametric study on the B-field pattern was carried out by changing the dimensions of the pole face model. In addition, the detailed trajectories of the dominant ion species produced in the beam line were calculated. The electrical and cooling parameters of the coil assembly were also estimated.

• Applied Science and Convergence Technology
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• 제23권5호
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• pp.265-272
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• 2014

The ion energy distributions (IEDs) arriving at a substrate strongly affect the etching rates in plasma etching processes. In order to determine the IEDs accurately, it is important to obtain the characteristics of radio frequency (rf) sheath at pulsed rf substrates. However, very few studies have been conducted to investigate pulsing effect on IEDs at multiple rf driven electrodes. Therefore, in this work, we extended previous one-dimensional dynamics model for pulsed-bias electrodes. We obtained the IEDs using the developed rf sheath model and observed that numerically solved IEDs are in a good agreement with the experimental results.

• Mass Spectrometry Letters
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• 제10권1호
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• pp.27-31
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• 2019

In this article, the properties of ${CO_2}^+(CO_2)_n$ clusters in a Paul ion trap have been investigated using mass-selective instability mode which conducted by chosen precursor ions, mainly $Ar^+$ and ${CO_2}^+$ produced by a mixture of Ar and $CO_2$. Exposure of ${CO_2}^+$ ions to $CO_2$ molecules, lead to the formation of ${CO_2}^+(CO_2)_n$ clusters. Here, Ar gas react as a buffer gas and lead to form ${CO_2}^+(CO_2)_n$ cluster by collisional effect.

• Molecules and Cells
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• 제42권3호
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• pp.262-269
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• 2019

The porcine myeloid antimicrobial peptide (PMAP), one of the cathelicidin family members, contains small cationic peptides with amphipathic properties. We used a putative lysozyme originated from the bacteriophage P22 (P22 lysozyme) as a fusion partner, which was connected to the N-terminus of the PMAP36 peptide, to markedly increase the expression levels of recombinant PMAP36. The PMAP36-P22 lysozyme fusion protein with high solubility was produced in Escherichia coli. The final purified yield was approximately 1.8 mg/L. The purified PMAP36-P22 lysozyme fusion protein exhibited antimicrobial activity against both Gram-negative and Grampositive bacteria (Staphylococcus aureus, Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa, and Bacillus subtilis). Furthermore, we estimated its hemolytic activity against pig erythrocytes as 6% at the high concentration ($128{\mu}M$) of the PMAP36-P22 lysozyme fusion protein. Compared with the PMAP36 peptide (12%), our fusion protein exhibited half of the hemolytic activity. Overall, our recombinant PMAP36-P22 lysozyme fusion protein sustained the antimicrobial activity with the lower hemolytic activity associated with the synthetic PMAP36 peptide. This study suggests that the PMAP36-P22 lysozyme fusion system could be a crucial addition to the plethora of novel antimicrobials.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2005년도 Proceedings of ISRS 2005
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• pp.22-25
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• 2005

The 10 Year Implementation Plan for a Global Earth Observation System of Systems (GEOSS), which was endorsed at the Third Earth Observation Summit in Brussels in February, 2005, emphasizes the importance of data management facilities for diverse and large-volume Earth Observation data from inhomogeneous information sources. A three year research plan for addressing this key target of GEOSS has just approved as the first step by the Japanese government. The goals of this research are, (1) to develop a data management core system consisting of data integration and information fusion functions and interoperability and information service functions; (2) to establish data and information flows between data providers and users; (3) to promote application studies of data integration and information fusion, especially in the fields of weather forecasting, flood forecasting, agricultural management, and climate variability and changes. The research group involves leading scientists on information science and technology, who have been developing giant data archive servers, storage area networks, metadata models, ontology for the earth observations. They are closely cooperating with scientists on earth sciences, water resources management, and agriculture, and establishing an effective collaborative research framework.