• Asian-Australasian Journal of Animal Sciences
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• v.25 no.5
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• pp.621-628
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• 2012

The FAT-1 protein is an n-3 fatty acid desaturase, which can recognize a range of 18- and 20-carbon n-6 substrates and transform n-6 polyunsaturated fatty acids (PUFAs) into n-3 PUFAs while n-3 PUFAs have beneficial effect on human health. Fat1 gene is the coding sequence from Caenorhabditis elegans which might play an important role on lipometabolism. To reveal the function of fat1 gene in bovine fetal fibroblast cells and gain the best cell nuclear donor for transgenic bovines, the codon of fat1 sequence was optimized based on the codon usage frequency preference of bovine muscle protein, and directionally cloned into the eukaryotic expression vector pEF-GFP. After identifying by restrictive enzyme digests with AatII/XbaI and sequencing, the fusion plasmid pEF-GFP-fat1 was identified successfully. The pEF-GFP-fat1 vector was transfected into bovine fetal fibroblast cells mediated by Lipofectamine2000$^{TM}$. The positive bovine fetal fibroblast cells were selected by G418 and detected by RT-PCR. The results showed that a 1,234 bp transcription was amplified by reverse transcription PCR and the positive transgenic fat1 cell line was successfully established. Then the expression level of fat1 gene in positive cells was detected using quantitative PCR, and the catalysis efficiency was detected by gas chromatography. The results demonstrated that the catalysis efficiency of fat1 was significantly high, which can improve the total PUFAs rich in EPA, DHA and DPA. Construction and expression of pEF-GFP-fat1 vector should be helpful for further understanding the mechanism of regulation of fat1 in vitro. It could also be the first step in the production of fat1 transgenic cattle.

• Korean Journal of Food Science and Technology
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• v.38 no.5
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• pp.712-716
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• 2006

Saccharomyces cerevisiae and Zygosaccharomyces rouxii were electrofused and fermented in germaniumfortified nutrients to produce high-yield, organic germanium. The conditions for the preparation of protoplasts from both strains and for electrofusion were studied. The protoplasts of both cells formed long pearl chains and the cell membranes were lysed and fused through cellulase and high frequency voltage $(450{\sim}750V/128{\sim}512\;{\mu}sec)$. The fusants with the fastest growth were selected, and then characterized for their carbohydrate usage and tolerance to glucose and salts. The glucose tolerance of the fusants was better than that of S. cerevisiae and similar to that of Z. rouxii. The fusants appeared to have resistance to 12% NaCl. The cell size of the fusants was greater than that of the parental strains. The fusant cells contained more gemlanium than the parental cells did. The electrofusion of S. cerevisiae and Z. rouxii increased the cell capacity and accumulation of germanium in the yeasts. This method was proved to be effective to produce a high quantity of organic germanium.

• Journal of the Korea Society of Computer and Information
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• v.19 no.1
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• pp.95-110
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• 2014

The purpose of this research is to further investigate the influence of partnership between with the mediator effect of the social business on the outcome of SCM. IT technology fusion electronic tags, mobile phone, such as cloud computing is also activated in supply chain management of recently, business is faster, if social business is applied here that are smarter, customers or suppliers, there may be communication directly and to further improve the relationship partnership. 150 questionnaires were sent to companies that have introduced SCM to their systems and are operating it. Among 150 questionnaires, 127 collected data were analyzed excluding incomplete 23 data. Statistical methods used in this study were frequency analysis, factor analysis, reliability analysis, t-test, ANOVA, path analysis, Scheffe test and Sobel test with Amos 18.0. and SPSS 21.0. The analytical results are as follows. First, the more the reliability, information share, continuous transaction, effects on the social business are getting higher, the interdependence has little impact on it. Second, the impact on the outcome of SCM, partnerships between companies, showed a significant influence the reliability, the share of information, the continuous transaction, but the interdependence was analysed as an uninfluential factor. Third, the social business is analyses to have a mediator effect in relationship between the partnership and the outcome of SCM.

• Korean Journal of Remote Sensing
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• v.35 no.6_4
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• pp.1391-1401
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• 2019

The KOMPSAT-3A is a high-resolution optical satellite launched in 2015 by Korea Aerospace Research Institute (KARI). KOMPSAT-3A provides Panchromatic (PAN-0.55 m), Multispectral (MS-2.2 m), and Mid-wavelength infrared (MIR_OR-5.5 m) image. However, due to security or military problems, MIR_OR image with 5.5m spatial resolution are provided down sampled at 33 m spatial resolution (MIR_rd). In this study, we propose spatial sharpening method to improve the spatial resolution of MIR_rd image (33 m) using virtual High Frequency (HF) image and optimal fusion factor. Using MS image and MIR_rd image, we generated virtual high resolution (5.5 m) MIR_OR^fus image and then compared them to actual high-resolution MIR_OR image. The test results show that the proposed method merges the spatial resolution of MS image and the spectral information of MIR_rd image efficiently.

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.69-76
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• 2011

This study describes the effect of myo-inositol on sustained cell division and plant regeneration from cotyledon-derived protoplast of cabbage (Brassica oleracea var. capitata). Freshly isolated protoplasts were cultured in modified Murashige and Skoog (MS) medium removed ammonia ions and containing $0.4\;mg\;l^{-1}$ thiamine HCl, $100\;mg\;l^{-1}$ myo-inositol, $2\;mgl^{-1}$ 2,4-D, $0.5\;mgl^{-1}$ BA, $30\;gl^{-1}$ sucrose and several concentrations of myo-inositol (2, 4, 6, 8, 10% (w/v)) as an osmotic stabilizer. After 3 weeks of culture in the dark at $25^{\circ}C$, the plating efficiency of cabbage protoplasts reached to $22.5{\pm}2.9%$ when cultured in modified MS medium supplemented with $2\;mgl^{-1}$ 2,4-D, $0.5\;mgl^{-1}$ BA, $30\;gl^{-1}$ sucrose and 8% (w/v) of myo-inositol at a density of $2{\times}10^5$ protoplasts/ml. Rapidly growing cell colonies after 3 weeks of culture were transferred to the same culture medium removed osmoticum. To induce shoot regeneration from calluses, calluses with about 2 mm in diameter were transferred to the MS medium containing $2\;mgl^{-1}$ BA and $0.5\;mgl^{-1}$ NAA. After further three weeks of incubation onto the medium in the light, green shoots were formed on the surface of calluses at a frequency of 30%. Upon transfer to half-strength MS basal medium, roots were formed onto the bottom of regenerated shoots without auxin treatments. These regenerated plantlets were successfully acclimatized to soil transfer, grown to normal mature plants. The cabbage protoplast culture system established in this study could be applied for production of somatic hybrids or cybrids by asymmetric protoplast fusion and mass proliferation of elite somatic clones of cabbage.

• Nuclear Engineering and Technology
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• v.42 no.6
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• pp.662-669
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• 2010

To develop the manufacturing methods for the blanket first wall (FW) of the International Thermonuclear Experimental Reactor (ITER) and to verify the integrity of the joint, Be/Cu mockups were fabricated and tested at the KoHLT-1 (Korea Heat Load Test facility), a graphite heater facility located at the Korea Atomic Energy Research Institute (KAERI). Since Be and Cu joining is the focus of the present study, the fabricated mockups had a CuCrZr heat sink joined with three Be tiles as an armor material, unlike the original ITER blanket FW, which has a stainless steel structure and coolant tubes. Hot isostatic pressing (HIP) was carried out at $580^{\circ}C$ and 100 MPa for 2 hours as the method for Be/Cu joining. Three interlayers, namely, $1{\mu}mCr/10{\mu}mCu$, $1{\mu}mTi/0.5{\mu}mCr/10{\mu}mCu$, and $5{\mu}mTi/10{\mu}mCu$ were applied as a coating to the Be tiles by a physical vapor deposition (PVD) method. A shear test was performed with the specimens, which were fabricated by the same methods as those used to fabricate the mockups. The average values were 125 MPa to 180 MPa, and the samples with the $1{\mu}mCr/10{\mu}mCu$ interlayer showed the lowest value. No defect or delamination was found in the joints of the mockups by the developed ultrasonic test using a flat-type probe with a 10 MHz frequency and a 0.25 inch diameter. High heat flux (HHF) tests were performed at $1.0\;MW/m^2$ heat flux for each mockup using the given conditions, and the results were analyzed by ANSYS-CFX code. For the test criteria, an expected fatigue lifetime about 1,000 cycles was obtained by analysis with ANSYS-mechanical code. Mockups using the interlayers of $1{\mu}mTi/0.5{\mu}mCr/10{\mu}mCu$ and $5{\mu}mTi/10{\mu}mCu$ survived up to 1,100 cycles over the required number of cycles. However, one of the Be tiles in the other two mockups using the $1{\mu}mCr/10{\mu}mCu$ interlayer was detached during the screening test, and others were detached by discharge after 862 cycles. The integrity of the joints using the proposed interlayers was proven by the HHF test, but the other interlayer requires more study before it can be used for the joining of Be to Cu. Moreover, it was confirmed that the measured temperatures agreed well with the analysis temperatures, which were used to estimate the lifetime and that the developed facility showed its capability of the long time operation.

• Journal of Digital Convergence
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• v.17 no.6
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• pp.401-408
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• 2019

The purpose of this study was to identify the relationship analysis between safety consciousness, self-efficacy, and life-respect according to participation in survival swimming program of elementary school students for interdisciplinary convergence research. In order to achieve this purpose, 248 elementary school students were selected from registered in Kangwon-do area by the cluster sampling method. Frequency analysis, confirmatory factor analysis, reliability analysis, correlations analysis and structural equation model analysis were executed using SPSS Statistics 23.0 Version and AMOS 23. As the results, First, it was discovered that the Safety Consciousness had static influence to the Self-Efficacy. Second, it showed that the Safety Consciousness did not gave static influence to the Life-Respect. Finally, it showed that the Self-Efficacy had static influence to the Life-Respect. In conclusion, the safety consciousness of elementary school students participating in the survival swimming program influences self-efficacy. It also has a positive effect on respect for life through self-efficacy. Therefore, various approaches to survival swimming education program are needed from the perspective of interdisciplinary fusion research.

• Journal of Korean Home Economics Education Association
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• v.33 no.2
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• pp.81-93
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• 2021

The purpose of this study was to analyze the image, perception, and preference type of high school students of Hanbok and examine the differences according to gender and participation in Hanbok classes. For this purpose, a survey was conducted with 438 high school students in Busan. Frequency analysis, chi-square test, t-test, and F-test were performed using the SPSS and the results were as follows. Firstly, for most high school students, Hanbok has an elegant image, but it was also confirmed that they perceive Hanbok to have a splendid image due to its exposure in mass media and SNS. The image of Hanbok differed by gender and participation in Hanbok classes. A higher percentage of male students than female students perceived Hanbok to have rustic image. Secondly, the type of hanbok that high school students prefer was traditional Hanbok, followed by fusion Hanbok, casual Hanbok, and fashion Hanbok. Hanbok preference type differed by gender and participation in classes. Group participating in hanbok classes showed a higher preference for fashion Hanbok than those who did not. Thirdly, high school students' perception of traditional Hanbok was above average. By subcomponents, it was shown in the order of 'esthetics', 'sustainability', and 'practicality'. It was found that there were statistically significant differences in traditional Hanbok perception according to gender and participation in Hanbok classes. Female students evaluated more positively for traditional Hanbok than male students and students who participate in Hanbok classes evaluated traditional Hanbok more positively than non-participating students.

• Korean Journal of Agricultural and Forest Meteorology
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• v.24 no.4
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• pp.234-243
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• 2022

In Korea, hail damage occurs every year, and in the case of agriculture, it causes severe field crop and cultivation facility losses. Therefore, it is necessary to develop a hail information service system customized for Korea's primary production and crop-growing areas to minimize hail damage. However, the observation of hail is relatively more difficult than that of other meteorological variables, and the available data are also spatially and temporally variable. A hail information service system was developed to understand the temporal and spatial distribution of hail occurrence. As part of this, a hail observation database was established that integrated the observation data from Korea Meteorological Administration with the information from newspaper reports. Furthermore, a hail risk map was produced based on this database. The risk map presented the nationwide distribution and characteristics of hail showers from 1970 to 2018, and the northeastern region of South Korea was found to be relatively dangerous. Overall, hail occurred nationwide, especially in the northeast and some inland areas (Gangwon, Gyeongbuk, and Chungbuk province) and in winter, mainly on the north coast and some inland areas as graupel (small and soft hail). Analyzing the time of day, frequency, and hailstone size of hail shower occurrences by region revealed that the incidence of large hail stones (e.g., 10 cm at Damyang-gun) has increased in recent years and that showers occurred mainly in the afternoon when the updraft was well formed. By integrating multidisciplinary data, the temporal and spatial gap in hail data could be supplemented. The hail risk map produced in this study will be helpful for the selection of suitable crops and growth management strategies under the changing climate conditions.

• Journal of the Korea Society of Computer and Information
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• v.28 no.1
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• pp.49-54
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• 2023

The Global Positioning System (GPS) was developed for military purposes and developed as it is today by opening civilian signals (GPS L1 frequency C/A signals). The current satellite orbits the earth about twice a day to measure the position, and receives more than 3 satellite signals (initially, 4 to calculate even the time error). The three-dimensional position of the ground receiver is determined using the data from the radio wave departure time to the radio wave Time of Arrival(TOA) of the received satellite signal through trilateration. In the case of navigation using GPS in recent years, a location error of 5 to 10 m usually occurs, and quite a lot of areas, such as apartments, indoors, tunnels, factory areas, and mountainous areas, exist as blind spots or neutralized areas outside the error range of GPS. Therefore, in order to acquire one's own location information in an area where GPS satellite signal reception is impossible, another method should be proposed. In this study, IMU(Inertial Measurement Unit) combined with an acceleration and gyro sensor and a geomagnetic sensor were used to design a system to enable location recognition even in terrain where GPS signal reception is impossible. A method to track the current position by calculating the instantaneous velocity value using a 9-DOF IMU and a geomagnetic sensor was studied, and its feasibility was verified through production and experimentation.