• Journal of the Korean Applied Science and Technology
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• v.37 no.1
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• pp.91-101
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• 2020

The purpose of this study was to investigate the influence of obesity on the expression of autophagy-related proteins in cardiac muscle. To this end, obesity was induced in rats through 20 weeks of high-fat diet, and the animals were then subjected to 8 weeks of treadmill exercise. Subsequently, the expression of proteins that regulate the induction of autophagy, formation of autophagosome, and fusion of autophagosome and lysosome was confirmed. Obesity was induced in the experimental animals (SD rats) through 20 weeks of high-fat diet (carbohydrate: 20%, fat: 60%, and protein: 20%), and they were subsequently subjected to 8 weeks of treadmill exercise (5 days/week, 30 min/day, 5 minutes; 8m/min, 5 minutes; 11m/min, 20 minutes; 14m/min). The experimental groups comprised the normal diet control group (ND-CON, n=10), high-fat diet comparison group (HFD-CON, n=10), and high-fat exercise group (HFD-TE, n=10). Oral glucose tolerance test was conducted before and after 8 weeks of treadmill exercise, and the area under the curve (AUC) was calculated. Through fasting insulin and fasting glucose levels, HOMA-IR, which is an index of insulin resistance, and abdominal visceral fat/body weight (AVF/BW) were calculated for comparison. Moreover, autophagy-related proteins were analyzed from cardiac tissue to investigate the effects of exercise training. Obesity was successfully induced in the HFD-CON group through long-term high-fat diet, and the HFD-CON group had higher body weight, AUC, HOMA-IR, and AVF/BW compared to the ND-CON group. The HFD-TE group, which underwent 8 weeks of treadmill exercise, showed improvements in AUC, HOMA-IR, and AVF/BW. Although the body weight tended to decrease as well, there was no statistically significant difference. mTOR and AMPK, which are involved in the induction of autophagy, both decreased in obesity but increased upon exercise. Beclin-1, BNIP3, ATG-7, p62, and LC3, which are related to the formation of autophagosomes, all increased in obesity and decreased after exercise. Cathepsin L and LAMP2, which regulate the fusion of autophagosome and lysosome, both decreased in obesity and increased upon exercise. Physical activity, including treadmill exercise, was found to induce normal autophagy and improve pathological phenomena observed in metabolic diseases. Therefore, the findings suggest the need to consider treadmill exercise as a primary means to achieve effective prevention and treatment of cardiac diseases.

• Agricultural and Biosystems Engineering
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• v.3 no.2
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• pp.79-84
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• 2002

The seawater involving diverse chemical elements was mixed with wasted coals to improve the quality. The thermal and chemical characteristics of these seawater-mixed coals were investigated and compared with those of original coals. The contents of MgO, $Na_2O$, and $K_2O$ were increased by mixing seawater in wasted coals. The content of $Na_2O$ in these coal samples was greatly increased due to the sodium, which was the main component of seawater. Thus, it was expected that fusion temperatures of these coal samples were decreased. Coal samples mixed with seawater showed that the rapid weight loss was started at the lower temperature than those of original coal samples. In these coal samples, the temperatures of maximum heat emission were lowered by average $61^{\circ}C.$. Thus, it is suggested that some chemical constituents of the seawater act an important role on lowering the ignition temperature of wasted coal. By mixing seawater into wasted coals, the calorific values were increased. Especially, calorific values were greatly increased in the coal samples of lower quality as Baksan A and B with the improvement of 15~20%.

• Transactions of Materials Processing
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• v.24 no.4
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• pp.256-263
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• 2015

Electric resistance spot welding has been used to join overlapped steel sheets in automotive bodies. Recently to reduce weight in automotive vehicles, non-ferrous metals are being used or considered in car bodies for hoods, trunk lids, doors parts, etc. Various welding processes such as laser welding, self-piercing rivet, friction stir welding are being used. In the current study, a new electric resistance heated friction stir spot welding is suggested for the spot welding of non-ferrous metals. The welding method can be characterized by three uses of heat -- electric resistance heating, friction stir heating and conduction heating of steel electrodes -- for the fusion joining at the interfacial zone between the two sheets. The welding process has variables such as welding current, diameter of the steel electrodes, revolutions per minute (rpm) of the friction stir pin, and the insert depth of the stir pin. In order to obtain the optimal welding variables, which provide the best welding strength, many experiments were conducted. From the experiments, it was found that the welding strength could be reached to the required production value by using an electrode diameter of 10mm, a current of 7.6kA, a stirring speed of 400rpm, and an insert depth of 0.8mm for the electric resistance heated friction stir spot welding of 5052 aluminum 1.5mm sheets.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2010.07a
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• pp.408-410
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• 2010

본 논문에서는 새로운 척도에 기반한 다노출 영상 융합 방법을 제안한다. 제안하는 방법에서는 각 노출정도에 따른 픽셀값의 그래프가 컨벡스 형태를 갖는다는 성질과 대조 값의 차이를 고려한 MRF (Markov Random Field) 기반의 에너지 함수를 설계하고 그 에너지 함수를 그래프컷 (Graph cut) 으로 풀어 각 노출치 영상에 대한 가중치 맵 (weight map)을 형성한다. 그리고 가중치 맵을 곱한 각 영상을 더함으로써 융합된 영상을 얻는다. 제안한 컨벡스 성질을 기반으로 한 척도는 특정 컬러 성분이 다른 컬러 성분보다 먼저 과노출 상태에 도달 한 경우의 영역을 가중치 계산에서 제외할 수 있기 때문에 기존의 가중치 기반의 방법보다 정확한 가중치 맵을 형성할 수 있게 한다. 실험 결과 제안하는 방법은 기존의 다노출 영상 융합에 비해 보다 넓은 영역에서 원 영상의 정보를 더 잘 표현하는 것을 확인하였다.

• Korean Journal of Artificial Intelligence
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• v.6 no.1
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• pp.1-10
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• 2018

Compressed Air is an important energy source used in most factories nowadays. The automation trend using air compressor has been gradually increasing with the interest of the 4th industry in recent years. With the air compressor system, it is possible to construct the device at low cost and easily achieve automation and energy saving. In addition, With trend of FA, miniaturation and light weight manufacturing trend expand their use in the electronics, medical, and food sectors. Research method is to design the technology for the remote control of the following information as USN base. Development of flexible sensing module from real time observation module for fusion of IT technology in compressed air systems, design and manufacture of flexible sensing module, and realiability assessment. Design of real-time integrated management system for observation data of compressed air system - Ability to process observation data measured in real time into pre-processing and analysis data. This study expects unconventionally decreasing effect of energy cost that takes up 60~70% of air compressor layout and operation and maintenance management cost through USN(Ubiquitous Sensor Network) technology by using optimum operational condition from real time observation module. In addition, by preventing maintenance cost from malfunction of air compressor beforehand, maintenance cost is anticipated to cut back.

• Journal of Microbiology and Biotechnology
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• v.26 no.7
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• pp.1267-1277
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• 2016

Currently, enzymatic synthesis of lactulose, a synthetic prebiotic disaccharide, is commonly performed with glycosyl hydrolases. In this work, a new type of lactulose-producing biocatalyst was developed by displaying β-galactosidase from Bacillus stearothermophilus IAM11001 (Bs-β-Gal) on the surface of Bacillus subtilis 168 spores. Localization of β-Gal on the spore surface as a fusion to CotX was verified by western blot analysis, immunofluorescence microscopy, and flow cytometry. The optimum pH and temperature for the resulting spore-displayed β-Gal was 6.0 and 75℃, respectively. Under optimal conditions, it showed maximum activity of 0.42 U/mg spores (dry weight). Moreover, the spore-displayed CotX-β-Gal was employed as a whole cell biocatalyst to produce lactulose, yielding 8.8 g/l from 200 g/l lactose and 100 g/l fructose. Reusability tests showed that the spore-displayed CotX-β-Gal retained around 30.3% of its initial activity after eight successive conversion cycles. These results suggest that the CotX-mediated spore-displayed β-Gal may provide a promising strategy for lactulose production.

• Korean Journal Plant Pathology
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• v.14 no.3
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• pp.229-239
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• 1998

Antibodies were raised against fusion proteins of the N-terminus and a region containing the GDNQ (Gly-Asp-Asn-Gln) polymerase motif of the L (polymerase) protein of sonchus yellow net virus (SYNV). Immunoblot analyses using these antibodies revealed the presence of the L protein in purified SYNV preparations and in nuclear extracts from infected tobacco. The serological analyses and detection in a polyacrylamide gels suggested that the L protein is present in at least a 20 fold lower abundance than the G, N, M1 and M2 proteins, and has size corresponding to a molecular weight of over 200 kDa as predicted from nucleotide sequence data. Electron microscopy with gold-labelled antibodies was used to localize the N, M2, and G proteins of SYNV in thin sections of infected tissue. When sections of SYNV-infected tissue were treated with antisera against total SYNV proteins and N protein, gold label could be detected in both the viroplasms and in virus particles. With the anti-M2 protein antiserum, the gold label was strongly localized in the viroplasms but only limited labelling of the virus particle sonly. Limited labelling of the L protein was observed in the viroplasms and the virus particles, presumably because of the low abundance of L protein in the tissues.

• Transactions of Materials Processing
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• v.27 no.3
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• pp.145-153
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• 2018

Magnesium material could be widely used in the automotive industry because of its high strength to weight ratio, but the electric resistance spot welding process of magnesium sheets is difficult because of its low electric resistance and high thermal conduction and thermal expansion. For this reason, an electric resistance surface friction spot welding process using rotating dies is suggested for the spot welding of magnesium metal sheets. This welding method can be characterized by three heating methods: (1) electric resistance heating on contacted surface, (2) surface friction heating by rotating dies, and (3) thermal conduction heating from heated steel electrodes, for the fusion of metal at the interfacial zone between the two magnesium sheets. This welding process also has variables to explore, such as welding currents, diameters of the steel electrode, and rotating dies. It was found that the welding strength could reach industrial requirements by applying a welding current of 11.0kA, with steel electrodes of 12mm diameter, with rotating dies of 4.4 mm diameter, under the condition of a revolution speed of 1200rpm of rotating dies, for the surface friction spot welding process of AZ31 magnesium alloy sheets of 1.4mm thickness.

• Journal of Welding and Joining
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• v.29 no.1
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• pp.107-114
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• 2011

CP steel was developed to reduce the weight and increase the strength of car body. When it was welded using state-of-the-art disk laser welding, the effected of boron on the microstructure and hardness were investigated. Welding power was fixed at 3.5kW and welding speeds were 4,8 and 12m/min. Full penetration occurred in welding speed of 12m/min and weld bead was almost unchanged with boron contents. But the welding speed increased, the upper and lower bead were narrowed. In a welding speed of more than 8m/min, underfill defects were formed on the bead bottom. The hardness of weld zone was somewhat fluctuation in fusion zone and HAZ showed the highest hardness values. The hardness of each region showed little change with the boron contents, and softening phenomenon occurred in the HAZ near the base metal regardless of the boron contents.

• Korean Journal of Veterinary Research
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• v.34 no.4
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• pp.653-664
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• 1994

This study was carried out to investigate diabetic renal changes in cyclophosphamide(CY) treated nonobese diabetic(NOD) mice and to develop animal model of diabetic human nephropathy. The 8-week-old NOD and ICR mice were injected with cyclophosphamide intraperitoneally at 200mg/kg body weight and compared the chemical effects on these mice with the non-treated NOD and ICR mice respectively. The renal glomeruli in ICR, cyclophosphamide-treated ICR(ICR+CY), NOD and cyclophosphamide-treated NOD(NOD+CY) mice were observed by the light and electron microscopes. The results obtained were summarized as follows ; 1. Spontaneous incidences of diabetes mellitus in NOD mice were significantly promoted by dosing with cyclophosphamide. 2. Glomerulohypertrophy, proliferation of mesangium, partial thickening of glomerular basement membrane, and partial fusion of pedicels of podocyte were observed in NOD mice and NOD+CY mice. These changes were not observed in both ICR mice and ICR+CY mice. 3. The diabetic nephropathy observed in NOD+CY mice was more severe than that of non-treated NOD mice.